| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,081 |
| تعداد مقالات | 34,377 |
| تعداد مشاهده مقاله | 47,996,259 |
| تعداد دریافت فایل اصل مقاله | 78,920,786 |
بررسی اثر بیمارگری سویههای Bacillus thuringiensis بومی خاک‌های زراعی ایران روی پروانه برگ خوار مصری پنبه Spodoptera littoralis | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 10، دوره 7، شماره 1 - شماره پیاپی 13، شهریور 1398، صفحه 143-155 اصل مقاله (921.21 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2019.121888 | ||
| نویسندگان | ||
| زهرا معقولی فرد1؛ رسول مرزبان* 2؛ شهرام حسامی3؛ غلامرضا صالحی جوزانی4؛ رضا شرفی4 | ||
| 1بخش گیاه‌پزشکی، دانشکده علوم کشاورزی، واحد علوم و تحقیقات فارس، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| 2بخش تحقیقات کنترل بیولوژیک، ﻣﻮﺳﺴﻪ ﺗﺤﻘﻴﻘﺎت ﮔﻴﺎه ﭘﺰﺷﻜﻲ، ﺳﺎزﻣﺎن ﺗﺤﻘﻴﻘﺎت آﻣﻮزش و ﺗﺮوﻳﺞ ﻛﺸﺎورزی، ﺗﻬﺮان، اﻳﺮان | ||
| 3بخش گیاه‌پزشکی، دانشکده علوم کشاورزی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| 4بخش تحقیقات بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| پروانه برگخوار مصری پنبه (Boisduval) Spodoptera littoralis، آفتی همه چیزخوار با دامنه میزبانی وسیع است که همه ساله خسارت قابل توجهی به محصولات کشاورزی وارد میکند. مشخص شده است که همه سویههای Bacillus thuringiensis(Bt) روی این آفت مؤثر نیستند و سویههای کمی با توجه به توکسین‌های پروتئینی خود روی آن مؤثرند. هدف از اجرای تحقیق حاضر، دستیابی به سویههای Btبومی با قابلیت کنترل پروانه برگخوار مصری و بررسی تاثیر محیط کشت تکثیر باکتری بر قابلیت حشره‌کشی سویههای منتخب بود. بدین منظور، قدرت بیمارگری 118 سویه بومی Bt(رشد داده شده در محیط R2NB) روی لاروهای پنج روزه پروانه برگخوار مصری پنبه در دمای 27 درجه سلسیوس روی غذای مصنوعی بررسی و غربال شدند. درصد مرگ و میر 118 سویه باکتری با غلظت 103 اسپور در میلی‌لیتر نشان داد که چهار سویه GON–9، QM–2، GN–13 و QM–1 به ترتیب با 33/93، 70، 67/46 و 33/43 درصد، بالاترین میزان مرگ و میر و سویه هایGN–12 ،EN–2 ،GON–12 ،GON–7،CHI–2 ،AGI–7 ،AGI–3 وAGI–2 روی لاروهای S. littoralis بی‌تأثیر بودند. سپس سویههای مؤثر در دو محیط کشتNutrient Broth و R2NB تکثیر شده، روی آفت مذکور ارزیابی شدند. نتایج نشان داد چهار سویه منتخب در محیط کشت R2NB نسبت به NB روی لاروهای پروانه برگخوار مصری اثر کشندگی بیشتری داشتند و تفاوت آنها معنی‌دار بود. همچنین محتویات ژنهای cry خصوصا ژن cry2Ab در سویههای مؤثر مورد بررسی و ردیابی قرار گرفت. نتایج واکنش زنجیرهای پلیمراز نشان داد که سویههای QM–1، QM–2 و GON–9 حاوی ژن cry2Ab هستند. | ||
| کلیدواژه‌ها | ||
| قدرت بیمارگری؛ پروانه برگخوار مصری پنبه؛ محیط کشت؛ Spodoptera littoralis Bacillus thuringiensis؛ cry genes | ||
| عنوان مقاله [English] | ||
| Pathogenic effects of Bacillus thuringiensis native Iranian soil strains on Egyptian cotton leafworm Spodoptera littoralis Z. | ||
| نویسندگان [English] | ||
| Zahra Magholi Fard1؛ Rasoul Marzban2؛ Shahram Hesami3؛ Gholam Reza Salehi Jouzani4؛ Reza Sharafi4 | ||
| 1Plant Protection Department, Faculty of Agricultural Sciences, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran | ||
| 2Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Plant Protection Department, Faculty of Agricultural Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| 4Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| The Egyptian cotton leafworm is a polyphagous pest with a broad range of hosts causing annually significant damage to agricultural crops. It has been found that no all strain of Bacillus thuringiesis are effective on this pest and small numbers of the isolates are effective due to the contents of their crystalline proteins. The aim of the present study was to obtain native isolates of Bt that were capable of controlling and investigate the effect of growth medium of Bt on the insecticidal ability of selected isolates. Hence, the pathogenicity of 118 native isolates of B. thuringiesis cultivated in R2NB medium were determined on five–day–old larvae of Spodptera.littoralis at 27 °C on artificial diet. The mortality rate of 118 native isolates of Bt with a concentration of 103 spore ml–1 illustrated that the highest mortality rates belong to four isolates GON–9, QM–2, GN–13 and QM–1 with 93.33, 70, 46.67 and 43.43, respectively. In addition, GN–12, EN–2, GON–7, CHI–2, AGI–7, AGI–3 and AGI–2 isolates were ineffective on S. littoralis larvae. Then, the effective isolates grown in nutrient broth and R2NB medium were evaluated on mentioned pest. The results showed that the four selected isolates were more lethal on R2NB medium than NB on larvae of Egyptian cotton leafworm and their differences were significant. Also, the content of cry genes particularly cry 2Ab in effective isolates were examined and traced. The outcomes of polymerase chain reaction demonstrated QM–1, QM–2 and GON–9 have cry 2Ab. | ||
| کلیدواژه‌ها [English] | ||
| pathogenicity, Egyptian cotton leafworm, Bacillus thuringiesis, medium, cry gene | ||
| مراجع | ||
|
Abbott, W.S. 1925. A method for computing the effectiveness of an insecticide. Journal of Economic Entomology. 18: 265–267. Alfazairy, A.A., El–Ahwany, A.M.D., Mohamed, E.A., Zaghloul, H.A.H. & El–Helow., E.R. 2013. Microbial control of the cotton leafworm Spodoptera littoralis (Boisd.) by Egyptian Bacillus thuringiensis isolates. Folia Microbial, 58: 155–162. Aly, N.A.H., Soliman. E.A.M., El–Kawokgy, T. 2008. RAPD identification of local Bacillus thuringiensis isolates toxic to Spodoptera littoralis and Culex pipiens using universal primers for cry genes. Middle Eastern and Russian Journal of Plant Science Biotechnology, 2(2): 60–66. Arrizubieta, M., William, T., Caballero, P., Simon, O. 2014. Selection of a nucleopolygedrovirus isolate from Helicoverpa armigera as the basis for a biological insecticide. Pest Management Science, 70(6): 967–976. Arthur, F.H. & Philips, T.W. 2003. Stored–product insect pest management and control, pp. 341–358. In Y.H. Hui, B.L., Bruinsma, J.R., Gorham, W.K., Nip, P.S., Tong, P Ventresca., (eds.), Food plant sanitation. Marcel Dekker, New York. Azizoglu, U., Salehi Jouzani, G., Yilmaz, N., Baz, E. & Ozkok, D. 2020. Genetically modified entomopathogenic bacteria, recent developments, benefits and impacts: A review. Science of the Total Environment. PP.139–169. Bai, C., Degheele. D., Janses., S., Lambert., B. 1993. Activity of insecticidal proteins and strans of Bacillus thuringiensis against Spodoptera exempta (Walker). Journal of Invertebrate Pathology. 62: 211–215. Bravo, A., Gill., S.S., Soberón M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 49(4): 423–435. Bruce, M.J., Gatsi, R., Crickmore, N., Sayyed, A.H. 2007. Mechanisms of resistance to Bacillus thuringiensis in the Diamond back Moth. Biopesticides International. 3(1): 1–12. Burges, H.D. & Thompson, E.M. 1971. Standardization and assay of microbial insecticides. In: Burges, H.D., Hussey, N.W. (eds.), Microbial control of insects and mites. Academic., New York, p 709. Ҫakici, F.Ӧ., Sevim. A., Demirbağ, Z., Demir., İ. 2014. Investigation interal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) Larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry. 38: 99–110. Dahi, H.F. 2012. Efficacy of Bt transgenic egyption cotton varieties expressing cry1Ac and cry2Ab genes against Spodoptera littoralis (Boisd). Journal of American Sciences. 8(3): 457–463. Dulmage, H.T. 1970. Insecticidial activity of HD–1, a new isolate of Bacillus thuringiensis var. alesti. Journal of Invertebrate Pathology. 15: 232–239. Dutton, A., Romeis, J. Bigler, F. 2003. Assessing the risks of insect resistant transgenic plants on entomophagous arthropods: Bt–maize expressing Cry1Ab as a case study. Biocontrol. 48: 611–636. Dutton, A., Romeis, J., Bigler, F. 2005. Effects of Bt maize expressing Cry1Ab and Bt spray on Spodoptera littoralis. Entomologica Experimentalis Et Applicata. 114: 161–169. Federici, B.A. 1997. Baculovirus Pathogenesis. University of California at Riverside, Department of Entomology and Interdepartmental Graduate Program in Genetics, Riverside California. Greenplate, J.T. 1999. Quantification of Bacillus thuringiensis insect control protein Cry1Ac over time in Bollgard cotton fruit and terminals. Journal of Economic Entomology. 92: 1377–1383. Hatem, A.E., Aldebis, H.K., Osuna, E.V. 2011. Effects of Spodoptera littoralis granulovirus on the development and reproduction of cotton leafworm, S.littoralis. Biological Control. 59: 192–199. Hatem, A.E., Reda, A.M. Amer., Osuna. E.V. 2012. Combination effects of Bacillus thuringiensis Cry1Ac toxin and nucleopolyhedrovirus or granulovirus of Spodoptera littoralis on cotton leafworm. Eyptian Journal of Biological Pest control. 22(2): 115–120. Jafari, M., Noruzi, P., Malbobi, M.A., Valizadeh, M., Mohammadi, SA. 2009. Transformation of cry1Ab gene to sugar beet (Beta vulgaris L.) by Agrobacterium and development of resistance plant against Spodoptera littoralis. Sugar Beet Journal. 2 (24): 37–55. Jua´rez–Pe´rez, V.M., Ferrandis, M.D., Frutos, R. 1997. PCR–based approach for detection of novel Bacillus thuringiensis cry genes. Applied and Environmental Microbiology. 63: 2997–3002. Keller, M., Sneh, B., Strizhov, N., Prudovski, E., Regev, M., Koncz, C., Schell, J. Zilberstein. 1996. Digestion δ–endotoxin by midgut proteases may explain reduced sensitivity of advanced instars of Sodoptera littoralis to Cry1C. Insect Biochemistry and Molecular Biology. 26: 365–373. Khodaverdi, H., Sahragard, A., Amirmoafi, M., Mohaghegh, J. 2010. Study of demographic parameters of Egyptian cotton leafworm on artificial diet in laboratory conditions. Iranian Journal of Plant Protection Science. 41(1): 61–69. Lacey, L.A., Frutos, R., Kaya, H.K., Vail, P. 2001. Insect pathogens as biological control agents: do they have a future? Biological Control. 21: 230–248. Lacey, L.A., Grzywacz, D., Shapiro–Ilan, D.I., Frutos, R., Brownbridge, M. and Goettel, M.S. 2015. Insect pathogens as biological control agents: Back to the future, Journal of Invertebrate pathology. 132: 1–41. Liao, C., Heckel, D.G., Akhurst, R. 2009. Toxicity of Bacillus thuringiensis insecticidial proteins for Helicoverpa armigera and Helicoverpa punctigera (Lepidoptera: Noctuidae), major pests of cotton. Journal of Invertebrate Pathology. 80: 55–66. Madani, S. 2016. Get to know the Egyptian cotton leafworm better. http://fspmarket.com/tutoralis/Spodoptera–littoralis–bois/ [Accessed 5 July 2016]. Magholifard, Z., Heami, Sh., Marzban, R., Salehi Jouzani, G. 2018. Pathogenic effects of three isolates of Nucleopolyhedrovirus, Spodoptera littoralis NPV, Helicoverpa armigera NPV, Spodoptera litura NPV on life stages of Egyptian cotton leafwormSpodoptera littoralis. Entomology and Phytopathology. 85(2):203–218. Magholifard, Z., Heami, Sh., Marzban, R., Salehi Jouzani, G. 2020. Individual and combined biological effects of Bacillus thuringiensis and Multicapsid Nucleopolyhedrovirus on the biological stages of Egyptian cotton leafworm, Spodoptera littoralis (B.) (Lep.:Noctuidae). Journal of Agricultural Science and Technology. 22(2):465–476. Mansour, S.A., Foda, M.S., Aly, A.R. 2012. Mosquitocidal activity of two Bacillus bacterial endotoxins combined with plant oila and conventional insecticides. Industrial Crops and Products. 35: 44–52. Marzban, R. 2002. Comparison bioassay of several native strains of Btand kurstaki serotype on Plodia interpunctella. Journal of Plant Diseases and protection. 70: 29–36. Marzban, R., Salehi Jouzani, G. 2006. Isolation of native Bacillus thuringiensis Berliner isolates from the agricultural soils of Iran. Journal of Agricultural Science. 1(2): 47–54. Marzban R., Saberi F., Shirazi M.M., 2016. Microfiltration and Ultrafiltration of Bacillus thuringiensis Fermentation broth: Membrane performance and spore–crystal recovery approaches. Brazilian Journal Chemical Engineering. 33 (4): 783–791. Nazerian, A., Jahangiri, R., Salehi Jouzani, G., Seifinejad, A., Soheilivand, S. Bagheri, O., Keshavarzi, M., Alamisaeid, K. 2009. Coleoptera–specific and putative novel cry genes in Iranian native Bacillus thuringiensis collection. Journal of Invertebrate Pathology. 102: 101–109. Novan A. 1985. Spodoptera littoralis. PP. 469–475. In Singh, P. and Moore, R. F. (eds.) Handbook of Insect Rearing. Elsivire. Amesterdam. Qayyum, M.A., Wakil, W., Arif, M.J., Sahi, Sh.T. 2015. Bacillus thuringiensis and Nuclear Polyhedrosis Virus for the Enhanced Bio–cotrol of Helicoverpa armigera. International Journal Agricultural and Biology. 17(5): 1043–1048. Robinson, G.S., Ackery., P.R. Kitching, I.J., Beccaloni, G.W., Hernández, L.M. 2010. Hosts a database of the world Lepidopteran hostplants. Natural History Museum, London. Available from http://www. nhm.ac.uk/hosts [Accessed 14 January 2012]. Salama, H.S., Foda, M.S., Sharaby, A. 1989. A proposed new biological standard for bioassay of bacterial insecticides versus Spodoptera spp. Tropical Pest Management. 35: 326–330. Salehi Jouzani, G., Abad, A.P., Seifinejad, A., Marzban, R., Kariman, K., Maleki, B. 2008. Distribution and diversity of Dipteran–specific cry and cyt genes in native Bacillus thuringiensis strains oBtained from different ecosystems of Iran. Journal of Industrial Microbiology & Biotechnology. 35(2): 83–94. Salehi Jouzani, G., Valijanian, E. and Sharafi, R. 2017. Bacillus thuringiensis: a successful insecticide with new environmental features and tidings. Applied Microbiology Biotechnology. 101(7): 2691–2711. SAS Institute. 1999. SAS Online Doc®. Version 8. Cary, NC. Seifinejad, A., Salehi Jouzani, G., Hosseinzadeh, A., Abdmishani, C. 2008. Characterization of Lepidoptera–active cry and vip genes in Iranian Bacillus thuringiensis strain collection. Biological Control. 44(2): 216–226. Sorour, M.A., Khamiss, O., Abd El–Wahab, A.S., El–Sheikh., M.A.K., Abul–Ela, S. 2011. An Economically modified semi–synthetic diet for mass rearing the Egyptian Cotton LeafWorm Spodoptera littoralis. Academic Journal of Entomology. 4(3): 118–123. SPSS. 1998: SPSS User’s Guide. SPSS, Inc., Chicago. Svobodová, Z., Romeis, J., Habuštová, O.S., Meissle, M. 2016. Susceptibility of Spodoptera littoralis (Boisd.) to lepidopteran active Cry proteins in stacked Bt maize. Available from https: //www.researchgate.net/publication/304019535 [accessed 12 January 2018]. Ullah, L., Asif, M., Arslan, M., Ashfaq, M. 2014. Temporal expression of Cry1Ab/c protein in Bt–cotton varieties, their efficacy against Helicoverpa armigera (Lepidoptera: Noctuidae) and population dynamics of suckingarthropods on them. International Journal of Agricultural and Biology. 16: 879–885. Wei, J.Z., Hale, K., Carta, L., Platzer, E., Wong, C., Fang, S., Aroian, R.V. 2003. Bacillus thuringiensis crystal proteins that target nematodes. Proceeding of the National Academy of Sciences. 100(5): 2760–2765. Xu, J., Shelton, A.M., Cheng, X. 2001. Variation in susceptibility of Diadegma insulare to permenthrin. Journal of Economic Entomology. 99: 541–546. | ||
|
آمار تعداد مشاهده مقاله: 501 تعداد دریافت فایل اصل مقاله: 323 |
||