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سلیمانی, سحر, لکزیان, امیر, فتوت, امیر, رمضانپور, محمود. (1399). بررسی تولید بیوسورفاکتانت توسط کنسرسیوم باکتریایی جدا شده از خاک آلوده به مواد نفتی. سامانه مدیریت نشریات علمی, 8(1), 55-71. doi: 10.22092/sbj.2020.121884
سحر سلیمانی; امیر لکزیان; امیر فتوت; محمود رمضانپور. "بررسی تولید بیوسورفاکتانت توسط کنسرسیوم باکتریایی جدا شده از خاک آلوده به مواد نفتی". سامانه مدیریت نشریات علمی, 8, 1, 1399, 55-71. doi: 10.22092/sbj.2020.121884
سلیمانی, سحر, لکزیان, امیر, فتوت, امیر, رمضانپور, محمود. (1399). 'بررسی تولید بیوسورفاکتانت توسط کنسرسیوم باکتریایی جدا شده از خاک آلوده به مواد نفتی', سامانه مدیریت نشریات علمی, 8(1), pp. 55-71. doi: 10.22092/sbj.2020.121884
سلیمانی, سحر, لکزیان, امیر, فتوت, امیر, رمضانپور, محمود. بررسی تولید بیوسورفاکتانت توسط کنسرسیوم باکتریایی جدا شده از خاک آلوده به مواد نفتی. سامانه مدیریت نشریات علمی, 1399; 8(1): 55-71. doi: 10.22092/sbj.2020.121884

بررسی تولید بیوسورفاکتانت توسط کنسرسیوم باکتریایی جدا شده از خاک آلوده به مواد نفتی

زیست شناسی خاک
مقاله 6، دوره 8، شماره 1، خرداد 1399، صفحه 55-71    اصل مقاله (380.21 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2020.121884
نویسندگان
سحر سلیمانی1؛ امیر لکزیان* 2؛ امیر فتوت2؛ محمود رمضانپور3
1دانشجوی دکترای بیولوژی خاک دانشگاه فردوسی مشهد، مشهد، ایران
2استاد گروه خاکشناسی دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
3استادیار مرکز تحقیات کشاورزی و منابع طبیعی مازندران
چکیده
آسنفتن، یکی از اجزای طبیعی نفت خام و ترکیبی مقاوم در برابر تجزیه و سرطانزا است. زیست پالایی که تکنیک استفاده از ریزجانداران زنده جهت تجزیه و معدنی کردن آلاینده­ها است، برای حذف آلودگی­های مقاوم محیطی نظیر آسنفتن استفاده می­شود. تجزیه کامل هیدروکربن­ها نتیجه فعالیت ترکیبی از گونه­های مختلف ریزجانداران بوده که به عنوان کنسرسیوم شناخته می­شود. از سوی دیگر، حلالیت کم آلاینده­های نفتی عامل محدود کننده زیست­پالایی است. به همین دلیل اخیراً استفاده از کنسرسیوم­های باکتریایی تولید کننده بیوسورفاکتانت، جهت افزایش دسترسی زیستی آلاینده­های نفتی، در فرآیند زیست­پالایی بسیار مورد توجه قرار گرفته است. در مطالعه حاضر، با استفاده از غنی­سازی خاک آلوده به مشتقات نفتی، باکتری­های تجزیه کننده آسنفتن جداسازی و سپس با بررسی میزان کشش سطحی محیط حاوی جدایه­ها، انواع جدایه­های باکتریایی مولد بیوسورفاکتانت غربال شدند. پس از شناسایی جدایه­ها، تجزیه آسنفتن توسط جدایه­های منفرد انتخابی، کنسرسیوم آن­ها و بیوسورفاکتا­نت­های استخراج شده از جدایه­ها در دو محیط کشت و خاک بررسی شد. نتایج نشان داد که دو جدایه AP3 و BM1 علاوه بر توانایی تجزیه آسنفتن، مولد بیوسورفاکتانت بوده و به ترتیب 2/72 و 54% و کنسرسیوم حاصل از آن­ها نیز 100% از آلاینده را در محیط محلول تجزیه می­کنند. بیوسورفاکتانت استخراج شده از جدایه AP3 در تجزیه آسنفتن مؤثرتر از بیوسورفاکتانت حاصل از جدایه BM1 عمل کرد. میزان تجزیه آسنفتن در خاک توسط جدایه AP3 و کنسرسیوم به ترتیب 7/64 و 7/75% بوده و استفاده از بیوسورفاکتانت AP3 به همراه کنسرسیوم نیز موجب تجزیه کامل آسنفتن شد. شناسایی جدایه­ها نشان داد که AP3 به میزان 100 درصد با سویه Bacillus velezensis CR-502(T) و جدایهBM1  5/99 درصد با سویهPseudomonas putida ATCC 12633 مطابقت دارد. بنابراین، استفاده از کنسرسیوم و کاربرد بیوسورفاکتانت به همراه آن، در مقایسه با تلقیح جدایه منفرد موجب بهبود تجزیه زیستی آسنفتن شد.
کلیدواژه‌ها
آلودگی‌های نفتی؛ باسیلوس؛ زیست‌پالایی؛ سودوموناس
عنوان مقاله [English]
Study of Biosurfactant Production by Bacterial Consortium Isolated from Oil Contaminated Soil
نویسندگان [English]
sahar soleymani1؛ Amir Lakzian2؛ A. Fottovat2؛ Mahmoud Reza Ramezanpour3
1Ph.D. student of Soil Science Department, Agricultural College, Ferdowsi University of Mashhad
2fProfessor of Soil Science Department, Agricultural College, Ferdowsi University of Mashhad
3Associated professor of Mazandaran Agricultural and Natural Resources Research and Education Center
چکیده [English]
Acenaphthene, a natural component of crude oil, is a carcinogenic compound and persistent to degradation. Bioremediation, which involves the use of living microorganisms for degradation and mineralization of contaminants, used to remove persistent contaminants such as Acenaphthene. Complete degradation of hydrocarbons is the result of different species activity that is known as a bacterial consortium. On the other hand, the low solubility of oil pollutants is a limiting factor in bioremediation. So, recently biosurfactant-producing bacterial consortia have been considered in the bioremediation to increase the bioavailability of oil contaminants. In the present study, Asenaphthene degrading bacteria were isolated by enriching the oil-contaminated soil and then, the biosurfactant-producing bacteria were screened based on the surface tension of the medium containing the isolates. After bacterial identification, degradation of Acenaphthene by isolates, consortium, and extracted biosurfactants from isolates, were investigated in the aqueous medium and soil. The results showed that both AP3 and BM1 could decompose Acenaphthene and produce biosurfactants. The AP3, BM1, and their consortium degraded 72.2%, 54%, and 100% of pollutants in the aqueous medium, respectively. The effect of biosurfactant extracted from AP3 on the degradation of Acenaphthene was more than the BM1 biosurfactant. Percentage of Acenaphthene degradation in soil by AP3 and consortium was 64.7% and 75.7% respectively, and the use of AP3 biosurfactant with the consortium led to complete degradation. 16S rRNA gene sequences of isolates showed that AP3 100% was compatible with Bacillus velezensis CR-502 (T) and BM1 99.5% was compatible with Pseudomonas putida ATCC 12633. Therefore, the use of a consortium and biosurfactants improve the biodegradation of Acenaphthene compared to inoculation of individual isolates.
کلیدواژه‌ها [English]
Bacillus sp, Bioremediation, Hydrocarbon contamination, Pseudomonas sp
مراجع
  1. Abdel-Mawgoud, A.M. Lepine, F. and Deziel, E. 2010.Rhamnolipids: diversity of structures, microbial origins and roles. Applied Microbiology and Biotechnology 86 (5): 1323-1336.
  2. Adebusoye, S.A. Ilori, M.O. Amund, O.O. Teniola, O.D. and Olatope, S.O. 2007. Microbial degradation of petroleum hydrocarbons in a polluted tropical stream. World Journal of Microbiology and Biotechnology 23: 1149–1159.
  3. Bai, N. wang, Sh. Abuduaini, R. Zhang, M. Zhu, X. and zhao, Y. 2017. Rhamnolipid-aided biodegradation of carbendazim by Rhodococcus sp.D-1: Characteristics, products, and phytotoxicity. Science of the Total Environment 590-591: 343-351.
  4. Batista, S.B. Mounteer, A.H. Amorim, F.R. and Totola, M.R. 2006. Isolation and characterization of biosurfactant/bioemulsifier producing bacteria from petroleum contaminated sites. Bioresource technology 97: 868-875.
  5. Bremner, J.M. and Mulvaney, C.S. 1982. Methods of soil analysis. American Society of Agronomy Inc. Madison, Wisconsin USA.
  6. Bezza, F.A. and Chirwa E.M.N. 2017. The role of lipopeptide biosurfactant on microbial remediation of aged polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. Chemical Engineering Journal 309: 563-576.
  7. Bisht, S. Pandey, P. Kaur, G. Aggarwal, H. Sood A. Sharma S. Kumar V. and Bisht, N.S. 2014. Utilization of endophytic strain Bacillus sp. SBER3 for biodegradation of polyaromatic hydrocarbons (PAH) in soil model system. European Journal of Soil Biology 60: 67-76.
  8. Bodour A., and Miller-Maier R. M. 1998. Application of a modified drop collapse technique for surfactant quantification and screening of biosurfactant-producing microorganism. Journal of Microbiological Methods 32: 273-280.
  9. Bouyoucos, G.J. 1962. Hydrometer Method Improved for Making Particle Size Analysis of Soils. Agronomy Journal. 54: 464-465.
  10. Bushnell L.D. and Haas H.F. 1941. TheUtilization of Certain Hydrocarbons by Microorganisms. Journal of  bacteriology 41:653-671.
  11. Chagas-Spinelli, A.C.O. Kato, M.T. de Lima, E.S. and Gavazza. 2012. Bioremediation of a tropical clay soil contaminated with diesel oil. Journal of Environmental Management 113: 510-516.
  12. Chebbi, A. Hentati, D. Zaghden, H. Baccar, N. Rezgui, F. Chalbi, M. Sayadi, S. and Chamkha, M. 2017. Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas sp. strain from used motor oil-contaminated soil. International Biodeterioration and Biodegradation 122: 128- 140.
  13. Chen, L. Li, G. and Zu-Liang, Ch. 2010. Biodegradation of naphthalene by strain Bacillus fusiformis (BFN). Journal of Hazardous Materials 182: 771–777.
  14. Chen, S.Y. Wei, Y.H. and Chod, J.S. 2007. Repeated pH-salt fed-batch fermentation for rhamnoloipid production with indigenous Pseudomonas aeruginosa S2. Applied Microbiology and Biotechnology 76: 67-74.
  15. Das, K. Mukherjee, A.K. 2005. Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples. Applied Microbiology and Biotechnology 69: 192–199
  16. Das, K. Mukherjee, A.K. 2007. Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresource Technology 98 (7): 1339-1345.
  17. Das, P. Mukherjee, S. and Sen, R. 2008. Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by biosurfactant producing bacterium of marine origin. Chemospher 72: 1229-1234.
  18. Dastgheib, S.M.M. Amoozegar, M.A. Elahi, E. Asad, S. Banat, I.M. 2008. Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnology Letters 30 263-270.
  19. Desai, J.D. and Banat, I.M. 1997. Microbial production of surfactants and their commercial potential. Microbiology and Molecular Biology Reviews 61, 47–64.
  20. Ellis, B. Harold, P. Kronberg, H. 1991. Bioremediation of a creosote contaminated site. Environmental Technology 12: 447-459.
  21. Eriksson, M. Sodersten, E. Yu, Z. Dalhammar, G. and Mohn, W.W. 2003. Degradation of polycyclic aromatic hydrocarbons at low temperature under aerobic and nitrate-reducing conditions in enrichment cultures from northern soils. Applied and Environmental Microbiology 69: 275-284.
  22. Eskandari, S. Hoodaji, M. Tahmourespour, A. Abdollahi, A. Mohammadian, Baghi T. Eslamian, S. and Ali-Askari, K.O. 2017. Bioremediation of Polycyclic Aromatic Hydrocarbons by Bacillus Licheniformis ATHE9 and Bacillus Mojavensis ATHE13 as Newly Strains Isolated from Oil-Contaminated Soil. Journal of Geography, Environment and Earth Science International 11(2): 1-11.
  23. Fonts, G.C. Amaral, P.F.F. Nele, M. and Coelho, M.A.Z. 2010. Factorial design to optimize biosurfactant production by Yarrowia lipolytica. Journal of Biomedicine and Biotechnology 2010: 1-8.
  24. Ghazali, F.M. Adul Rahman, R.N.Z. Salleh, A.B. and Basri, M. 2004. Biodegradation of hydrocarbons in soil by microbial consortium. International Biodeterioration and Biodegradation 54: 61-67.
  25. Ghosal, D. Dutta, A. Chakraborty, J. Basu, S. and Dutta, T.K. 2013. Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W. Research in Microbiology 164: 155-163.
  26. Gomes, M.B. Gonzales-Limache, E.E. Sousa, S.T.P. Dellagnezze, B.M. Sartoratto, A. Silva, L.C.F. Gieg, L.M., Valoni, E. Torre,s A.P.R. Sousa, M.P. De Paula, S.O. Silva, C.C. and Oliveira, V.M. 2018. Exploring the potential of halophilic bacteria from oil terminal environments for biosurfactant production and hydrocarbon degradation under high-salinity conditions. International Biodeterioration and Biodegradation 126: 231-242.
  27. Grimberg, S.J. Stringfellow, W.T. and Aitken, M.D. 1996. Quantifying the biodegradation of phenanthrene by Pseudomonas stutzeri P16 in the presence of a nonionic surfactant. Applied and Environmental Microbiology 62 (7): 2387–2392.
  28. Hamzah, A. Sabturani, N. and Radiman. 2013. Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria. Sains Malaysiana 42 (5): 615-623.
  29. Holtman, M. Kobayashi, D. 1997. Identification of Rhodococcus erythropolis isolates capable of degrading the fungicide carbendazim. Applied Microbiology and Biotechnology 47: 578–582.
  30. Hu, X. Wang, C. and Wang, P. 2015. Optimization and characterization of biosurfactant production from marine Vibrio sp. Strain 3B-2. Frontiers in Microbiology 6: 1-110.
  31. Hwang, G. Park, S.R. Lee, C.H. Ahn, I.S. Yoon, Y.J. and Mhin, B.J. 2009. Influence of naphthalene biodegradation on the adhesion of Pseudomonas putida NCIB 9816-4 to a naphthalene-contaminated soil. Journal of Hazardous Materials 171: 491–493.
  32. Keith, L.H. and Telliard, W.A. 1979. Priority pollutants I-a perspective view. Environmental Science and Technology 13: 416-423.
  33. Khalifeh, A. Roozbehani, B. and Mashinchian Moradi, A. 2013. Optimization of biosurfactant in oily polluted waters clearance recovery. American Journal of Oil and Chemical Technologies 1 (3): 1-3.
  34. Lewandowska, A. and Walorczyk, S. 2010. Carbendazim residues in the soil and their bioavailability to plants in four successive harvests. Polish Journal of  EnvironmentalStudies 19: 757–761.
  35. Li, L. Li, Q. Li, F. Shi, Q. Yu, B. Liu, F. and Xu, P. 2006. Degradation of carbazole and its derivatives by a Pseudomonas sp. Applied Microbiology and Biotechnology 73: 941-948.
  36. Ling, J. Zhang, G. Sun, H. Fan, Y. Ju, J. and  Zhang, Ch. 2011. Isolation and characterization of a novel pyrene-degrading Bacillus vallismortis strain JY3A. Science of the Total Environment, 409: 1994–2000.
  37. Liu, C.W. and Liu, H.S. 2011. Rhodococcus erythropolis strain NTU-1 efficiently degrades and traps diesel and crude oil in batch and fed-batch bioreactors. Process Biochemistry 46: 202–209.
  38. Lotfabad, T.B. Sourian, M. Roostazad, R. Najafabadi, A.R. Adelzadeh, M.R. and Noghabi, K.A. 2009. An efficient biosurfactant-producing bacterium Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran. Colloids and Surfaces B: Biointerfaces 69: 183-193.
  39. Makkar, R.S. and Cameotra, S.S. 1997. Biosurfactant production by thermophilic Bacillus subtilis strain. Journal of Industrial Microbiology and Biotechnology 18 (1): 37-42.
  40. Mao, J. Lou, Y. Teng, Y. and Li, Zh. 2016. Bioremediation of polycyclic aromatic hydrocarbon-contaminated soil by a bacterial consortium and associated microbial community changes. International Biodeterioration and biodegradation 70: 141-147.
  41. Mao, J. Luo, Y. Teng, Y. and Li, Zh. 2012. Bioremediation of polycyclic aromatic hydrocarbon contaminated microbial community changes. International Biodeterioration and Biodegradation 70: 141-147.
  42. Marmur, J. 1961. A procedure for the isolation of detoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3: 208-218.
  43. McFarland, J. 1907. Nephelometer: An instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. Journal of the American Medical Association 14: 1176-1178.
  44. Mnif, I. Ellouze-chaabouni, S. Ayedi, Y. and Ghribi, Dh. 2015. Treatment of diesel- and kerosene-contaminated water by B. subtilis SPB1 biosurfactant-producing strain. Water EnvironmentResearch 86: 707-716.
  45. Mnif, I. Rihab, S. and Dhouha, Gh. 2017. Application of bacterial biosurfactant for enhanced removal and biodegradation of disel oil in soil using a newly isolated consortium. Process Safety and Environmental Protection 109: 72-81.
  46. Mnif, I. Sahnoun, R. Ellouze-Chaabouni, S and Ghribi, D. 2014. Evaluation of B. subtilis SPB1 biosurfactants' potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design. Environmental Science and Pollution Research 21 (2): 851-861.
  47. Mohanty, S. and Mukherji, S. 2013. Surfactant aided biodegradation of NAPLs by Burkholderia multivorans: comparison between Triton X-100 and rhamnolipid JBR-515. Colloids and Surfaces B: Biointerfaces 102: 644–652.
  48. Montagnolli, R.N. Matos Lopes, P.R. and Bidoia, E.D. 2015. Assessing Bacillus subtilis biosurfactant effects on the biodegradation of petroleum products. Environmental Monitoring and Assessment 187(1): (4116) 1-17.
  49. Mukherjee, A.K. and Bordoloi, N.K. 2012. Biodegradation of Benzene, Toloene and Xylene (BTX) in liquid culture and in soil by Bacillus subtilis and Pseudomonas aeruginosa strains and a formulated bacterial consortium. Environmental Scince and Pollution Research 19 (8): 3380-3388.
  50. Nelson, D.W. and Sommers, L.E. 1982. Methods of soil analysis. American Society of Agronomy Inc. Madison, Wisconsin USA.
  51. Ohadi, M., Dehghan-Nudeh GH.R., Shakibaie M., Banat I.M., Pournamdari M. and Forootanfar H. 2017. Isolation, characterization and optimization of biosurfactant production by an oil-degrading Acinetobacter junii B6 isolated from an Iranian oil excavation site. Biocatalysis and Agricultural Biotechnology, 12:1-9.
  52. Olsen, S.R. and Sommers, L.E. 1982. Methods of soil analysis. American Society of Agronomy Inc. Madison, Wisconsin USA.
  53. Pal, M.P. Vaidya, B.K. Desai, K.M. and Joshi, R.M. 2009. Media optimization for biosurfactant production by Rhodococcus erythropolis MTCC 2794: Artificial intelligence versus a statistical approach. Journal of Industrial Microbiology 36(5): 747-756.
  54. Parvaresh, B.V. Soniyamby, A.R. Mariappan C. Kavithakumari, P. Palaniswamy, M. and Lalitha, S. 2011. Biosurfactant production by Psedomonas sp from soil using whey as carbon source. New York Science Journal 4(4): 100-103.
  55. Patowary, K. Patowary, R. Kalita, M.C. and Deka, S. 2016. Development of an Efficient Bacterial Consortium for the Potential Remediation Consortium for potential remediation of hydrocarbons from contaminated sites. Frontiers in Microbiology 7: 1092.
  56. Patowary, R. patowary K. Kalita, M.C. and Deka, S. 2018. Application of biosurfactant for enhancement of bioremediation process of crude oil contaminated soil. International Biodeterioration and Biodegradation 129: 50-60.
  57. Pereira, J.F.B. Gudina, E.J. Costa, R. Vitorino, R. Teixeira, J.A. and Coutinho, J.A.P. 2013. Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. Fuel 111: 259-68.
  58. Pinyakong, O. Habe, H. Kouzuma, A. Nojiri, H. Yamane, H. and Omori, T. 2004. Isolation and characterization of genes encoding polycyclicaromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4. FEMS Microbiology Letters 238: 297-305.
  59. Poblete-Castro, I. Becker, J. Dohnt, K. dos Santos, V.M. and Wittmann, Ch. 2012. Industrial biotechnology of Pseudomonas putida and related species. Applied Microbiology and Biotechnology 93 (6): 2279-2290.
  60. Potin, O. Rafin, C. and Veignie, E. 2004. Bioremediation of an aged polycyclic aromatic hydrocarbons (PAHs)-contaminated soil by filamentous fungi isolated from the soil. International biodeterioration and biodegradation 54: 45-52.
  61. Rahaman, K.S.M. Thahira-Rahman, J. lakshmanaperumalsamy, P. and Banat, I.M. 2002. Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresource Technology 85 (3): 257-261.
  62. Robert, M. Mercade, M. E. Bosch, M. P. Parra, J. L. Espuny, M. J. Manresa, M. A. and Guinea, J.(1989). Effect of the carbon source on Biosurfactant Production by Psuedomonas aeruginosa 44T1. Biotechnology Letters 11: 871-874.
  63. Rufino, R.D. de Luna, J.M. de Campos Takaki, G.M. and Sarubbo, L.A. 2013. Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988. Electronic journal of biotechnology 17:34-38.
  64. Sanger, F.  and Coulson, A.R. 1975. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. Journal of Molecular Biology 94(3): 441-8.
  65. Sathishkumar, M. Binupriya, A.R. Baik, S-H. and Yun, S-E. 2008. Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas. Clean 36: 92–96.
  66. Schocken, M.J. and Gibson, D.T. 1984. Bacterial oxidation of polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene. Applied and Environmental Microbiology 48: 10-16.
  67. Shi, T. Fredrickson, J.K. and Balkwill, D.L. 2001. Biodegradation of polycyclic aromatic hydrocarbons by Sphingomonas strains isolated from the terrestrial subsurface. Journal of  Industrial Microbiology and Biotechnology 26: 283-289.
  68. Silva, J.E. Rocha, A. Silva, N.M.P. Rufino, R.D. and Luna, J.M. 2014. Characterization of a biosurfactant produced by Pseudomonas cepacia CCT6659 in the presence of industrial wasted and its application in the biodegradation of hydrophobic compounds in soil. Colloids and Surfaces B: Biointerfaces 117: 36-41.
  69. Singh, G.B. Gupta, S. and Gupta, N. 2013. Carbazole degradation and biosurfactant production by newly isolated Pseudomonas sp. Strain GBS.5. International Biodeterioration and Biodegradation 84: 35-43.
  70. Souza Vera, E.C. Vessoni-Penna T.C. and Souza Oliveira, R.P. 2014. Biosurfactant-enhanced hydrocarbon bioremediation: An overview. International Biodeterioration and Biodegradation 89: 88-94.
  71. Tzintzun-Camacho, O. Loera, O. Ramirez-saad, H.C. and Gutierrez-Rjas, M. 2012. Comparison of mechanisms of hexadecane uptake among pure and mixed cultures derived from a bacterial consortium. International Biodeterioration and Biodegradation 70: 1-7.
  72. Viisimaa, M. Karpenko, O. Novikov, V. Trapido, M. and Goi, A. 2013. Influence of biosurfactant on combined chemical-biological treatment of PCB-contaminated soil. Chemical Engineering Journal 220: 352-359.
  73. Wu, J.Y. Yeh, K.L. Lu, W.B. Lin, C.L. and Chang, J.S. 2008. Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil contaminated site. Bioresource Technology. 99: 1157-1163.
  74. Yin, H. Qjang, J. Jia, Y. Ye, J. Peng, H. Qin, H. Zhang, N. and  He, B. 2009. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochemistry 44: 302-308
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سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب