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تأثیر سویه‌های مختلف باکتری سودوموناس فلورسنس بر فاکتورهای مورفولوژیک، بیوشیمیایی و آنزیم‌های آنتی‌اکسیدان گیاه شوید (Anethum graveolens L.) تحت تنش سرب | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 5، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 227-244 اصل مقاله (841.28 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128076.2656 | ||
| نویسندگان | ||
| اکرم رهبری1؛ حمیده فاطمی* 2؛ بهروز اسماعیل پور3 | ||
| 1دانش‌آموخته کارشناسی ارشد، گروه باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 2دانشجوی دکتری، گروه باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 3دانشیار، گروه علوم باغبانی، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| چکیده | ||
| اخیراً روش‌های مختلفی برای کاهش اثرهای مخرب آلاینده‌ها مورد استفاده قرار می‌گیرد که کاربرد کودهای زیستی با اثرهای مثبت آنها بر خصوصیات خاک و محیط‌زیست می‌توانند جایگزینی مناسب برای بسیاری از این روش‌ها باشند. به‌منظور بررسی اثر دو سویه باکتری سودوموناس فلورسنس بر شاخص‌های رشد، فیزیولوژیک و بیوشیمیایی گیاه شوید (Anethum graveolens L.) در شرایط تنش سرب، یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1394 انجام شد. تیمارهای آزمایش شامل تنش سرب در دو سطح (صفر و ppm600) و تیمار تلقیح با باکتری در سه سطح (تلقیح با سویه‌های 150 و 159 سودوموناس فلورسنس به‌صورت جداگانه و بدون تلقیح یا شاهد) بود. نتایج حاصل از این مطالعه نشان داد که صفات رشدی تعداد برگ، تعداد ساقه، ارتفاع بوته، وزن تر و خشک اندام هوایی و وزن تر و خشک ریشه به‌طور معنی‌داری تحت تأثیر آلودگی سرب قرار گرفت و صفت وزن تر و خشک بوته، تعداد برگ و ارتفاع گیاه به‌ترتیب 22، 31، 19 و 9 درصد کاهش یافتند. محتوی پرولین و کربوهیدرات گیاه شوید تحت تأثیر حضور سرب افزایش معنی‌داری نشان داد، همچنین تلقیح با باکتری نیز محتوی پرولین و کربوهیدرات گیاه را افزایش داد که میزان این افزایش در تلقیح با سویه 150 چشمگیرتر بود. فعالیت آنزیم‌های آنتی‌اکسیدانی به‌صورت معنی‌دار تحت تأثیر سرب و باکتری قرار گرفت و حضور سرب به‌ترتیب موجب افزایش و کاهش فعالیت آنزیم پراکسیداز و کاتالاز شد، اما تلقیح با باکتری سودوموناس به‌ویژه سویه 150 سبب افزایش معنی‌دار فعالیت هر دو آنزیم شد. علاوه‌براین باکتری‌ها توانستند میزان سرب گیاه شوید را کاهش دهند. به‌طور کلی نتایج این پژوهش نشان داد که تلقیح با باکتری سودوموناس با افزایش رشد و بهبود فعالیت‌های آنزیمی در کاهش اثرهای تنش سرب بر گیاه شوید کارآمد بودند. | ||
| کلیدواژه‌ها | ||
| پرولین؛ عناصر سنگین؛ کودهای زیستی؛ وزن خشک | ||
| عنوان مقاله [English] | ||
| Effects of different strains of bacterium Pseudomonas fluorescens on morphological and biochemical factors and antioxidant enzymes activities of Dill (Anethum graveolens L.) under Pb stress | ||
| نویسندگان [English] | ||
| A. Rahbary1؛ H. Fatemi2؛ B. Esmailpour3 | ||
| 1M.Sc. student, Department of Horticultural Science, Mohaghegh Ardabili University, Ardebil, Iran | ||
| 2Ph.D. student, Department of Horticultural Science, Mohaghegh Ardabili University, Ardebil, Iran | ||
| 3Department of Horticultural Science, Mohaghegh Ardabili University, University, Ardebil, Iran | ||
| چکیده [English] | ||
| Recently, various methods have been used to alleviate the detrimental effects of pollutants. The application of biofertilizers with respect to their positive impacts on soil characteristics and environment can be served as a suitable alternative to many of these methods. In order to investigate the two-way effect of bacterium Pseudomonas fluorescens on plant growth, physiological and biochemical characteristics of dill (Anethum graveolens L.) under Pb stress conditions, a factorial experiment based on a completely randomized design with three replications was conducted at Mohaghegh Ardabili University. Experimental treatments included Pb stress at two levels (0 and 600 ppm) and bacterial inoculation at three levels (inoculation with Pseudomonas fluorescens strains150 and 159 separately and without inoculation (control)). The results of this study showed that the growth characteristics such as leaf number, plant height, and shoot fresh and dry weight were significantly affected by Pb contamination and shoot fresh and dry weight, leaf number and plant height decreased by 22, 31, 19 and 9%, respectively. The content of proline and carbohydrates in dill showed a significant increase under the influence of lead. Bacterial inoculation also increased the content of proline and plant carbohydrates, which was more pronounced in inoculation with strain150. The activity of antioxidant enzymes was significantly influenced by Pb contamination and bacterial inoculation. Pb increased peroxidase but decreased catalase activity, but inoculation with Pseudomonas bacteria, especially strain 150, significantly increased the activity of both enzymes. In addition, the bacteria were able to reduce the amount of Pb in dill. In general, the results of this experiment indicated that Pseudomonas bacterial inoculation was effective in alleviating the effects of Pb stress on dill via growth promotion and improving enzymatic activity. | ||
| کلیدواژه‌ها [English] | ||
| Proline, heavy metal, biofertilizers, dry weight | ||
| مراجع | ||
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