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اثر تنش خشکی بر محتوای فیتواسترول‌ها و ژن‌های کلیدی درگیر در مسیر سنتز بتاسیتوسترول در گیاه دارویی کدوی تخم‌کاغذی (Cucurbita pepo L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 4، دوره 36، شماره 2 - شماره پیاپی 100، خرداد و تیر 1399، صفحه 209-226 اصل مقاله (608.07 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128207.2658 | ||
| نویسندگان | ||
| محمد زینالی* 1؛ بهرام ملکی زنجانی2؛ پرویز مرادی3؛ فرید شکاری2؛ سید محسن نیازخانی4 | ||
| 1دانش‌آموخته دکترا، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه زنجان، زنجان، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه زنجان، زنجان، ایران | ||
| 3استادیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویح کشاورزی، زنجان، ایران | ||
| 4دانش‌آموخته دکترا، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه ارومیه، ارومیه، ایران | ||
| چکیده | ||
| تنش‌های زیستی ازجمله خشکی بر میزان تولید متابولیت‌های ثانویه به‌ویژه استرول‌های گیاهی (فیتواسترول‌ها) اثر می‌گذارد. در بین فیتواسترول‌های موجود در کدوی تخم‌کاغذی، بتاسیتوسترول از مهمترین مواد مؤثره در آن است که خواص دارویی زیادی نیز دارد. در این مطالعه، اثر پنج سطح تنش خشکی بر روی میزان تولید روغن بذر و فیتواسترول‌های موجود در آن در سه ژنوتیپ و رقم استریاکای کدوی تخم‌کاغذی (Cucurbita pepo L.) مطالعه شد. تجزیه فیتواسترول‌ها با استفاده از GC/MS نشان داد که افزایش میزان تنش خشکی اثر منفی بر میزان تولید روغن حاصل از بذر رسیده کدوی تخم‌کاغذی داشت ولی فیتواسترول‌های تشکیل‌دهنده روغن به‌ویژه بتاسیتوسترول افزایش یافتند و این افزایش در رقم استریاکا (Styriaca) از بقیه ژنوتیپ‌ها بیشتر بود. برای تأیید این نتایج، 30-15 روز بعد از گلدهی دانه‌های آن برداشت و میزان بیان ژن‌های SQS، PP2A، SMT2، ERG26 و Cycloartenol synthase درگیر در مسیر سنتز فیتواسترول‌ها بررسی شد. نتایج مشخص کرد که در بین سطوح مختلف تنش خشکی و ژن‌های مورد مطالعه، در سطح دیم‌کاری ژن‌ها بالاترین میزان بیان را داشته و در آن بازه زمانی، عمده فعالیت مسیر سنتز استروئیدها در کدوی تخم‌کاغذی مسیر سنتز بتاسیتوسترول و استیگماسترول است. | ||
| کلیدواژه‌ها | ||
| بتاسیتوسترول؛ کدوی تخم‌کاغذی (Cucurbita pepo L.)؛ تنش خشکی؛ بیان ژن؛ فیتواسترول؛ رقم استریاکا | ||
| عنوان مقاله [English] | ||
| Effects of drought stress on phytosterols content and expression of key genes involved in Beta sitosterol biosysthesis pathway in medicinal plant pumpkin (Cucurbita pepo L.) | ||
| نویسندگان [English] | ||
| M. Zeynali1؛ B. Maleki Zanjani2؛ P. Moradi3؛ F. Shekari2؛ S.M. Niazkhani4 | ||
| 1Department of Agronomy and Plant Breeding, Faculty of Agriculture, Zanjan University, Zanjan, Iran | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Zanjan University, Zanjan, Iran | ||
| 3Natural Resources Research Division, Agricultural and Natural Resources Research Center of Zanjan, Zanjan Iran | ||
| 4Department of Agronomy and Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| Biological stresses such as drought affect the production of secondary metabolites, especially plant sterols (phytosterols). Among the phytoestrols found in pumpkin seeds, betasitosterol is one of the most important components which also have many medicinal properties. In this study, the effect of five levels of drought stress on seed oil production and its phytosterols was studied in three genotypes and Styriaca variety of pumpkin (Cucurbita pepo L.). Analysis of phytosterols using GC/MS showed that increasing drought stress had a negative effect on oil production from pumpkin ripe seeds, but oil phytosterols, especially betasitosterol, increased and this increase was higher in Styriaca than other genotypes. To confirm these results, the seeds were harvested 15-30 days after flowering and the expression of SQS, PP2A, SMT2, ERG26 and Cycloartenol synthase genes involved in the phytosterol biosynthesis pathway was investigated. The results indicated that, among the different levels of drought stress and genes studied, highest expression level was observed at the rainfed level and at that time, betasitosterol and stigmasterol pathway had the main activity in steroid biosynthesis pathway in pumpkin. | ||
| کلیدواژه‌ها [English] | ||
| Beta sitosterol, Cucurbita pepo L, drought stress, gene expression, phytosterol, styriaca variety | ||
| مراجع | ||
|
- Ahmadi Avval, P., Mojab, F. and Noujavan, S., 2006. Quantitation of 7-sterols in seeds oil of Cucurbita pepo. Journal of Medicinal Plant, 3(23): 72-79. - Awad, A.B., Barta, S.L., Fink, C.S. and Bradford, P.G., 2008. Beta-Sitosterol enhances tamoxifen effectiveness on breast cancer cells by affecting ceramide metabolism. Molecular Nutrient and Food Research, 52: 419-426. - Backhouse, N., Rosales, L., Apablaza, C., Goïty, L., Erazo, S., Negrete, R., Theodoluz, C., Rodríguez, J. and Delporte, C., 2008. Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa, Buddlejaceae. Journal of Ethnopharmacology, 116: 263-269. - Bahreininejad, B., Razmjoo, J. and Mirza, M., 2014. Effect of water stress on productivity and essential oil content and composition of Thymus carmanicus. Journal of Essential Oil Bearing Plants, 17(5): 717-725. - Barzegar, R., Peyvast, Gh., Ahadi, A.M., Rabiei, B., Ebadi, A.A. and Babagolzadeh, A., 2013. Biochemical systematic, population structure and genetic variability studies among Iranian Cucurbita (Cucurbita pepo L.) accessions, using genomic SSRs and implications for their breeding potential. Biochemical Systematics and Ecology, 50: 187-198. - Beck, F.X., Neuhofer, W. and Muller, E., 2000. Molecular chaperones in the kidney: distribution, putative roles, and regulation. American Journal of Physiology-Renal Physiology, 279: F203-F215. - Blassberg, R. and Briscoe, J., 2018. Smoothened sensor places sodium and sterols center stage. Developmental Cell, 44(1): 3-4. - Bouchereau, A., Clossais, B.N., Bensaoud, A., Beport, L. and Renard, M., 1996. Water stress effects on rapeseed quality. European Journal of Agronomy, 5: 19-30. - Bouic, P.J., 2001. The role of phytosterols and phytosterolins in immune modulation: a review of the past 10 years. Current Opinion in Clinical Nutrition and Metabolic Care, 4(6): 471-475. - Chen, T.H.H. and Murata, N., 2002. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology, 5(3): 250-257. - Chen, M.X., Zhang, B., Li, C.X., Kulaveerasingam, H., Chew, F.T. and Yu, H., 2015. Transparent testa GLABRA1 regulates the accumulation of seed storage reserves in Arabidopsis. Plant Physiology, 169: 391-402. - Divsalarmary, M., Thamasbisarvestani, Z., Modaressanavi, A.M. and Hamidi, A., 2016. Study the effect of drought stress on oil, protein percent and fatty acids composition of soybean grain. Plant Ecophysiology. 8(27): 44-55. - Flexas, J. and Medrano, H., 2002. Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitations revisited. Annals of Botany, 89: 183-189. - Fruhwirth, G.O. and Hermetter, A., 2008. Production technology and characteristics of Styrian pumpkin seed oil. European Journal of Lipid Science Technology, 110: 637-644. - Gossell-Williams, M., Davis, A. and O'Connor, N., 2006. Inhibition of testosterone-induced hyperplasia of the prostate of Sprague-Dawley rats by pumpkin seed oil. Journal of Medicinal Food, 9: 284-286. - Habenicht, A.J.R., Glomset, J.A., Krng, W.C., Nist, C., Mitchell, C.D. and Ross, R., 1981. Early changes in phosphatidylinositol and arachidonic acid metabolism in quiescent SWISS 3T3 cells strmulated to divide by platelet-derived growth factor. The Journal of Biological Chemistry, 256: 12329-12335. - Haines, T.H., 2001. Do sterols reduce proton and sodium leaks through lipid bilayers? Prog Lipid Research. 40: 299-324. - Herchi, W., Harrabi, S. and Sebei, K., 2009. Phytosterols accumulation in the seeds of Linum usitatissimum L. Plant Physiology and Biochemistry, 47: 880-885. - Hosseini, S.H., Yousefzadeh, S., Yeritesayan, S. and Hemmati, K.H., 2016. Growth analysis and qualitative traits pumpkin (Cucurbita pepo L.) affected by application of chemical and organic fertilizer. Journal of Plant Production, 23(1): 131-155. - Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K. and Shinozaki, K., 1999. Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology, 17: 287-291. - Kim, M.Y., Kim, E.J., Kim, Y.N., Choi, C. and Lee, B.H., 2012. Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Research and Practice, 6: 21-27. - Kumar, M.S., Ali, K., Dahuja, A. and Tyagi, A., 2015. Role of phytosterols in drought stress tolerance in rice. Plant Physiology and Biochemistry, 96: 83-89. - Lagarda, M.J., Garc´ıa-Llatas, G. and Farre´, R., 2006. Analysis of phytosterols in foods. Journal of Pharmaceutical and Biomedical Analysis, 41: 1486-1496. - Livak, K.J. and Schmittgen, T.D., 2001. Analysis of relative gene expression data using Real-time quantitative PCR and the 2(T) (-Delta Delta C) method. Methods, 25: 402-408. - López Pereira, M., Sadras, V.O. and Trapani, N., 1999. Genetic improvement of Sunflower in Argentina between 1930 and 1995. I. Yield and its component. Filed Crops Research, 62: 157-166. - Philips, K.M., Ruggio, D.M. and Ashraf-Khorassani, M., 2005. Phytosterol composition of nuts and seeds commonly consumed in the United States. Journal of Agricultural and Food Chemistry, 53: 9436-9445. - Purcell, L.C., Serraj, R., Sinclair, T.R. and De, A., 2004. Soybean N2 fixation estimates, ureide concentration, and yield responses to drought. Journal of Crop Science, 44: 484-492. - Rabbi Angourani, H., Panahande Yangajeh, J., Boland Nazar, S.A., Saba, J. and Zare Nahandi, F., 2017. The effects of exogenous salicylic acid on some quantitative and qualitative attributes of medicinal pumpkin (Cucurbita pepo L. var. Styriaca) under drought stress. Advances in Bioresearch, 8(2): 242-249. - Rao, S.R. and Ravishankar, G.A., 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20: 101-153. - Razmjoo, Kh., Heydarizadeh, P. and Sabzalian, M.R., 2008. Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomile. International Journal of Agriculture & Biology, 10(4): 451-454. - Roche, J., Mouloungui, Z., Cerny, M. and Merah, O., 2016. Fatty acid and phytosterol accumulation during seed development in three oilseed species. International Journal of Food Science and Technology, 51(8): 1820-1826. - Ryan, E., Galvin, K., O’Connor, T.P., Maguire, A.R. and O’Brien, N.M., 2007. Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes. Plant Foods for Human Nutrition, 62: 85-91. - Sajed, M.A., Moghadem, H.H., Yazdani, D. and Ahmadi Avval, P., 1999. Effects of plastic mulches, spacing and phosphorus and potassium fertilizer levels on the growth and yield of common pumpkin, Cucurbita pepo convar pepo var. styriaca. Journal of Medicinal and Aromatic Plant Sciences, 21: 650-653. - Schaeffer, A., Bronner, R., Benveniste, P. and Schaller, H., 2001. The ratio of campesterol to sitosterol that modulates growth in Arabidopsis is controlled by Sterol Methyl transferase 2; 1. Plant Journal, 25: 605-615. - Schaller, H., 2004. New aspects of sterol biosynthesis in growth and development of higher plants. Plant Physiology, 42: 465-76. - Shafie Mashtany, S., Harachorloo, M. and Delkhosh, B., 2009. Physicochemical evaluation of oil extracted from different varieties of Iranian pumpkin seeds. Food Technology & Nutrition, 7(4): 57-68. - Singh, S. and Sinha, S., 2005. Accumulation of metals and its effects in Brassica juncea (L.) Czern. (cv. Rohini) grown on various amendments of tannery waste. Ecotoxicology and Environmental Safety, 62(1): 118-127. - Stefanoudaki, E., Chartzoulakis, K., Koutsaftakis, A. and Kotsifaki, F., 2001. Effect of drought stress on qualitative characteristics of olive oil of cv Koroneiki. Grasas y Aceites, 52(3-4): 202-206. - Tohidi Moghadam, H.R., Zahedi, H. and Ghooshchi, F., 2011. Oil quality of canola cultivars in response to water stress and super absorbent polymer application. Pesquisa Agropecuária Tropical, 41(4): 579-586. - Valitova, J.N., Sulkarnayeva, A.G. and Minibayeva, F.V., 2016. Plant sterols: diversity, biosynthesis, and physiological functions. Biochemistry, 81(8): 1050-1068. - Whitaker, B.D. and Gapper, N.E., 2008. Ripening-specific stigmasterol increase in tomato fruit is associated with increased sterol C-22 desaturase (CYP710A11) gene expression. Journal of Agricultural and Food Chemistry, 56: 3828-3835. - Xu, H.M., Kong, X.D., Chen, F, Huang, J.X., Lou, X.Y. and Zhao, J.Y., 2015. Transcriptome analysis of Brassica napus pod using RNA-Seq and identification of lipid-related candidate genes. BMC Genomics, 16(858): 1-10. - Yeagle, P.L., 2011. The Structure of Biological Membranes. CRC Press, 398p. - Yoshida, Y. and Niki, E., 2003. Antioxidant effects of phytosterol and its components. Journal of Nutritional Science and Vitaminology (Tokyo), 49(4): 277-280. - Zeynali, M., Maleki Zanjani, B., Moradi, P. and Shekari, F., 2019a. Effects of field capacity based-irrigation levels on physiological and agronomic characteristics of medicinal pumpkin (Cucurbita pepo L.). Applied Research in Field Crops, 31(4): 1-20. - Zeynali, M., Maleki Zanjani, B., Moradi, P. and Shekari, F., 2019b. Effects of drought stress on some physiological traits, yield and yield component in four varieties of pumpkin (Cucurbita pepo L.). Journal of Plant Research (Iranian Journal of Biology), 32(2): 439-452. | ||
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