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بررسی تاثیر پیش تیمار بذر با باکتری‌های محرک رشد گیاه بر فعالیت آنزیم‌های آنتی اکسیدانی گیاهچه و شاخص‌های جوانه‌زنی دو رقم گندم نان در شرایط تنش شوری | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 9، شماره 1 - شماره پیاپی 17، خرداد 1399، صفحه 27-44 اصل مقاله (1.61 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.122519.1215 | ||
| نویسندگان | ||
| افشین مظفری* 1؛ سجاد فتح اللهی2 | ||
| 1استادیار دانشگاه آزاد اسلامی واحد ایلام، گروه تخصصی زراعت و اصلاح نباتات، ایلام، ایران. | ||
| 2کارشناسی ارشد، دانشگاه آزاد اسلامی واحد ایلام، گروه تخصصی زراعت و اصلاح نباتات، ایلام، ایران. | ||
| چکیده | ||
| به‌منظور بررسی تاثیر بیوپرایمینگ بذر گندم با رایزوباکتری‌های محرک رشد گیاه (PGPR) بر فعالیت آنزیم‌های آنتی اکسیدانتی گیاهچه و شاخص‌های جوانه‌زنی دو رقم گندم نان تحت شرایط تنش شوری، آزمایشی در سال 1396 در آزمایشگاه دانشگاه آزاد اسلامی واحد ایلام به‌صورت فاکتوریل بر پایه طرح کاملاً تصادفی در 3 تکرار اجرا گردید. تنش شوری شامل: صفر (شاهد)، 50، 100 و 200 میلی‌مولار نمک کلرید سدیم، باکتری‌های PGPR شامل: عدم تلقیح بذر با PGPR (شاهد) و تلقیح بذر با PGPR و ارقام گندم شامل: تجن و افق بود. جنس و گونه‌های باکتری‌های PGPR مورد استفاده در این پژوهش شامل chroococcum Azotobacter، Azospirillum berasilense وpotida Pseudomonas بود که از موسسه تحقیقات خاک و آب کشور تهیه شد. صفات آزمایشی شامل فعالیت آنزیم‌های سوپراکسید دیسموتاز (SOD)، کاتالاز (CAT) و گلوتاتیون پرواکسیداز (GPX)، وزن خشک ریشه‌چه، ساقه‌چه و گیاهچه، درصد جوانه‌زنی، سرعت جوانه‌زنی و شاخص بنیه بذر بود. اثر ساده عوامل آزمایش بر تمام صفات بسیار معنی‌دار (01/0≥P) شد. نتایج آزمایش نشان داد که رقم افق نسبت به تجن از نظر شاخص‌های جوانه‌زنی، وزن خشک ریشه‌چه، ساقه‌چه و گیاهچه و فعالیت آنزیم‌های آنتی اکسیدانتی برتر بود. با افزایش شدت تنش شوری همه صفات روند کاهشی داشتند. تیمار پرایم بذر با PGPR در مقایسه با تیمار عدم مصرف باکتری‌های PGPR باعث بهبود صفات آزمایش شد. به‌طورکلی، نتایج آزمایش نشان داد، پرایمینگ بذور گندم با باکتری‌های PGPR در مقایسه با تیمار شاهد (عدم پرایمینگ بذر) از طریق بهبود شاخص‌های جوانه‌زنی و مقدار فعالیت آنزیم‌های آنتی اکسیدانتی گیاهچه تا حدودی آثار مخرب تنش شوری را کاهش داد. | ||
| کلیدواژه‌ها | ||
| باکتری‌های PGPR؛ شوری؛ آنزیم‌های آنتی اکسیدانتی؛ گندم؛ شاخص جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on Antioxidant Enzymes Activity of Seedling and Germination Indices of Two Wheat Cultivar Under Salt Stress Conditions | ||
| نویسندگان [English] | ||
| Afshin Mozafari1؛ Sajad Fathollahy2 | ||
| 1Assistant Professor, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran. | ||
| 2Masters student, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran | ||
| چکیده [English] | ||
| In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely randomized design with three replications in a laboratory of Islamic Azad University branch of Ilam, in 2017. Experimental factors included salt stress at four levels: zero (Control), 50, 100 and 200 mM sodium chloride, PGPR bacteria in two levels: non-inoculation with PGPR (control) and seed inoculation with PGPR and wheat cultivars include: Tajan and Ofogh. The genus and species of PGPR bacteria used in this study included Azotobacter chroococcum, Azospirillum berasilense and Pseudomonas potida, which were obtained from the Iranian soil and water research institute. Experimental traits included Activity of superoxide dismutase enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPX), dry weight of radicl, gomul and seedling, germination percentage, germination rate and seed vigor index. The main effect of experimental factors on all traits was highly significant (P≤0.01). The results showed that Ofogh was superior to Tajan cultivar for all germination indices, dry weight of radicl, gomul and seedling, Activity of antioxidant enzymes. With increasing salt stress, all of traits were decreasing. Priming seeds with PGPR bacteria increased the traits studied. In general, priming of wheat seeds with PGPR in comparison with control treatment (non-inoculation) by improving germination indices and Activity of antioxidant enzymes to some extent reduced the harmful effects of salinity stress. | ||
| کلیدواژه‌ها [English] | ||
| PGPR, Salinity, Antioxidant enzymes, Germination indices and Wheat | ||
| مراجع | ||
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Abdul-Baki, A.A., and J.D. Anderson. 1973. Vigor Determination in Soybean Seed by Multiple Criteria1. Crop Sci. 13: 630-633. Ananthi, M., P. Selvaraju, and K. Sundaralingam. 2017. Evaluation of Bioprimed Chilli Seeds under Salt Stress Condition. Chem. Sci. Rev. Lett. 6(23): 1735-1738. Anitha, D., T. Vijaya, and N.V. Reddy. 2013. Microbial endophytes and their potential for improved bioremediation and biotransformation: a review. Indo Am. J. Pharm. Res. 3: 6408–17. Anne, C.L., and T. Bruno. 1997. Glutathiowe-mediated regulation of ATP sulfurylase activity, SO42-, uptake and oxidative stress response in lntact canola roots. Plant Physiol. 114: 177-183. Ashraf, M., and P.J.C. Harris. 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166: 3-16. Azarnivand, H., Z. Ahmadi, and H. Nasseri. 2004. Investigation of salinity on germination of two species of Artemisia fragrans and Artemisia spicigera. J. Desert. 9 (2): 315-307. Basra, S.M.A., I.A. Pannu, and I. Afzal. 2003. Evaluation of seedling vigour of hydro and matriprimed wheat (Triticum aestivum L.) seeds. Int. J. Agric. Biol. 5: 121-123. Bennett, M.A. 1998. The use of biologicals to enhance vegetable seed quality. Seed Technol. 20: 198–208. Bewley, J.D., K.J. Bradford, H.W.M. Hilhorst, and H. Nonogaki. 2013. Seeds: physiology of development, germination and dormancy. 3. ed. New York: Springer. Bharathi, R., R. Vivekananthan, S. Harish, A. Ramanathan, and R. Samiyappan. 2004. Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Protec. 23: 835–843. 2004. Buriro, M., F.C. Oad, M.I. Keerio, S. Tunio, and A.W. Gandahi. 2011. Wheat seed germination under the influence of temperature regimes. Sarhad J. Agric. 27(4): 539-543. Celikkol Akcay, U., O. Ercan, M. Kavas, L. Yildiz, C. Yilmaz, H.A. Oktem, and M. Yucel. 2010. Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings. Plant Growth Regual. 61: 21-28. Cuartero, J., M.C. Bolarin, M.J. Asins, and V. Moreno. 2005. Increasing salt tolerance in tomato. J. Exp. Bot. 57: 1045-1058. Dantas, B.F., R. de Cássia Barbosa da Silva, R.C. Ribeiro, and C.A. Aragão. 2015. Respiration and antioxidant enzymes activity in watermelon seeds and seedlings subjected to salt and temperature stresses. Am. J. Exp. Agric. 7(2): 0-77. Das, A.B. 2013. Bio prospecting and genetic engineering of mangrove genes to enhance salinity tolerance in crop plants. In: S.M. Jain, and S.D. Gupta, Biotechnology of Neglected and Underutilized Crops. New York: Springer, p. 385-456. Dobbelaere, S., A. Croonenborghs, A. Thys, D. Ptacek, J. Vanderleyden, P. Dutto, C. Labendera- Gonzalez, J. Caballero-Mellado, J.F. Aguirre, Y. Kapulnik, S. Brener, S. Burdman, D. Kadouri, S. Sarig, and Y. Okon. 2001. Response of Agronomically important crops to inoculation with Azospirillum. Aus. J. Plant Physiol. 28: 871–879. Ejazrasll, A.W., and A. Rehman. 1997. Germination response of sensitive and tolerant sugarcane lines to sodium chloride. Seed Sci. Technol. 25: 465-471. Entesari, M., F. Sharifzadadh, M. Dashtaki, and M. Ahmadzadeh. 2013. Effects of Biopriming on the Germination Traits, Physiological Characteristics, Aantioxidant Enzymes and Control of Rhizoctonia solani of a Bean Cultivar (Phaseolus vulgaris L.). Iranian J. Field Crop Sci. 44(1): 35-45 (In Persian, with English Abstract) Fan, H.F., C.X. Du, L. Ding, and Y.L. Xu. 2013. Effects of nitric oxide on the germination of cucumber seeds and antioxidant enzymes under salinity stress. Acta Physiol. Plant. 35(9): 2707- Foyer, C.H., H. Lopez-Delgado, J.F. Dat, and I.M. Scott. 1997. Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling. Physiol. Plant. 100: 241–254 Foyer, C.H., and G. Noctor. 2005. Oxidant and antioxidant signaling in plants: A re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell and Environ. 28: 1056-1071. Gale, J. 1970. Growth of Atriplex halimus L. in sodium chloride salinated cultured solution as effected by the relative humidity of the air. Aust. J. Biol. Sci. 23: 947-952. Gholami A., S. Shahsavani, and S. Nezarat. 2009. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Acad. Sci. Eng. Technol. 49: 19–24. Ghiasi, A., S. Parsa, A. Hamidi, and K. Khavazi. 2012. The effect of priming methods on germination and vigure of wheat seedling and the population of the bacteria on the seed. J. Seed Sci. Technol. 1: 38-25. Gill, S.S., and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930. Han, H.S., and K.D. Lee. 2005. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis mineral uptake and growth of lettuce under soil salinity. Res. J. Agric. Biol. Sci. 1(3): 210-215. Harter, L.S.H., F.S. Harter, C. Deuner, G.E. Meneghello, and F.A. Villela. 2014. Effect of salinity on physiological performance of mogango seeds and seedlings. Hort. Brasi. 32(1): 80-85. Hasanuzzaman, M., K. Nahar, and M. Fujta. 2013. Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad, P. et al. (Eds.). Ecophysiology and responses of plants under salt stress. New York: Springer. 25-87. Huang, J., and R.E. Remann. 1995. Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Can. J. Plant Sci. 75: 815-819. Kasim, W.A., M.E. Osman, M.N. Omar, I.A. Abd El-Daim, S. Bejai, and J. Meijer. 2013. Control of drought stress in wheat using plant-growth promoting bacteria. J. Plant Growth Regul. 32: 122–130. Kloepper, J.W., R.M. Zablotowicz, E.M. Tipping, and R. Lifshitz. 1991. Plant growth promoting mediated by bacterial rhizosphere colonizers. pp: 315-326. In: D.L. Keister, and P.B. Cregan (Eds.). The rhizosphere and plant growth. Kluwer Academic Publishers, Netherlands. Larkindale, J.D., J.R. Hall, M. Knight, and E. Vierling. 2005. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling sathways in the acquisition of thermo-tolerance. Plant Physiol. 138: 882-897. Lugtenberg, B., T. Chin-A-Woeng, and G. Bloemberg. 2002. Microbe–plant interactions: principles and mechanisms. Antonie van Leeuwenhoek, 81: 373–383. McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Sci. Technol. 27: 177–237. Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177. Mahmood, A., O.C. Turgay, M. Farooq, and R. Hayat. 2016. Seed biopriming with plant growth promoting rhizobacteria. FEMS Microbiol. Ecol. 92(8): 1-14. Majidi, M.M., M.R. Jazayeri, and Gh. Mohammadinejad. 2009. Salt effects on germination and seedling growth of sainfoin (Onobrychis viciifolia Scop.) genotypes. Iranian J. Range. Forest Plant Breed Genet. Res. 17(2): 256-269. Maslenkova, L.T., T.S. Miteva, and P. Popoval. 1999. Changes in the polypeptide patterns of barley seedling exposed to jasmonic acid and salinity. Plant Physiol. 98: 700-707. Minami, M., and H. Yoshikawa. 1979. A simplified assay method of superoxide dismutase activity for clinical use. Clin. Chim. Acta. 92: 337–342. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Tren. in Plant Sci. 7(9): 405-410. Mittler, R., S. Vanderauwera, M. Gollery, and F. Van Breusegem. 2004. Reactive oxygen gene network of plants. Trend. Plant Sci. 9: 490-498. Moeinzadeh A., F. Sharif-Zadeh, M. Ahmadzadeh, and F. Heidari-Tajabadi. 2010. Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed in vigoration and seedling growth. Aust. J. Crop Sci. 4: 564. Momeni, A. 2010. Geographical Distribution and Salinity Levels of Iranian Soil Resources. Iranian J. Soil Res. (Soil and Water Sci.). 24(3): 203-215. Mozaffari, A. 2013. Physiological evaluation of drought tolerance in two cultivars of bread wheat inoculated with plant growth promoting rhizobacteria (PGPR). Ph.D. thesis. Islamic Azad University, Takestan Branch, Iran. Mozaffari, A. 2014. Evaluation the effects of plant growth promoting rhizobacteria (PGPR) on SOD, MDA and Proline content in two wheat cultivars. 20th World Congress of Soil Science. June 8-13, Jeju, Korea. Naidoo, G., and Y. Naidoo. 2001. Effects of salinity and nitrogen on growth, ion relations and proline accumulation in Triglochin bulbosa. Wetl. Ecol. Manag.9: 491-497 Navrot, N., V. Collin, J. Gualberto, E. Gelhaye, M. Hirasawa, P. Rey, D.B. Knaff, E. Issakidis, J.P. Jacquot, and N. Rouhier. 2006. Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. Plant Physiol. 142: 1364-1379. Niranjan, S.R., S.A. Deepak, P. Basavaraju, H.S. Shetty, M.S. Reddy, and J.W. Kloepper. 2003. Comparative performance of formulations of plant growth promoting rhizobacteria in growth promotion and suppression of downy mildew in pearl millet. Crop Prot. 22: 579–588. Niranjan, S.R., N.P. Shett, and H.S. Shetty. 2004. Seed bio-priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew. Int. J. Pest Manag. 50(1): 41-48. Nourmohammadi, Gh., S.A. Siadat, and A. Kashani. 2007. Cereal Crops. Shahid Chamran University of Ahvaz Press. pp. 45-163. Paglia, D.E., and W.N. Valentine. 1967. Studies on the qualitative and quantitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70: 158-169. Pinheiro, D.T., da A.L. Silva, L.J. da Silva, M.C. Sekita, and D.C.F. dos Santos Dias. 2016. Germination and antioxidant action in melon seeds exposed to salt stress. Agropec. Trop., Goiânia. 46: 336-342. Pitzschke, A., C. Forzani, and H. Hirt. 2006. Reactive oxygen species signaling in plants. Antioxid. Redox. Signal. 8: 1757-1764. Raju, N.S., S.R., Niranjana, G.R. Janardhana, H.S. Prakash, H.S. Shetty, and S.B. Mathur. 1999. Improvement of seed quality and field emergence of Fusarium moniliforme infected sorghum seeds using biological agents. J. Sci. Food Agri. 79: 206-212. Rhee, S.G. 2006. H2O2, a necessary evil for cell signaling. Sci. 312: 1882-1883. Seckin, B., I. Turkan, A.H. Sekmen, and C. Ozfidan. 2010. The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barley grass) and Hordeum vulgare L. (cultivated barley). Environ. Exp. Bot. 69(1): 76-85. Sharma, P., A.B. Jha, R.S. Dubey, and M. Pessarakli. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J. Bot. 1(1): 1-26. Shaukat, K., S. Affrasayab, and S. Hasnain. 2006a. Growth responses of Helianthus annus to plant growth promoting rhizobacteria used as a biofertilizer. J. Agri. Res. 1(6): 573-581. Shaukat, K., S. Affrasayab, and S. Hasnain. 2006b. Growth responses of Triticumn aestivum to plant growth promoting rhizobacteria used as a biofertilizer. Res. J. Microb. 1(4): 330-338. Sivritepe, N., H.Ö. Sivritepe, I. Türkan, M. Bor, and F. Özdemir. 2008. NaCl pre-treatments mediate salt adaptation in melon plants through antioxidative system. Seed Sci. Technol. 36 (2): 360-370. Yacoubi, R., C. Job, M. Belghazi, W. Chaibi, and D. Job. 2013. Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress. Seed Sci. Res. 23(2): 99-110. Yao, Z., L. Liu, F. Gao, C. Rampitsch, D.M. Reinecke, J.A. Ozga, and B.T. Ayele. 2012. Developmental and seed aging mediated regulation of antioxidative genes and differential expression of proteins during pre- and post-germinative phases in pea. J. Plant Physiol. 169(5): 1477-1488. Zakeri, AK, O.R. Nikzad, M. Yasayi, SH. Sarikhani Khorrami, And M.J. Mino. 2012. Ofogh of wheat cultivar suitable for planting in temperate and relatively warm regions of the country with water or soil salinity. Fars Jihad-Agricultural Agricultural Research Center. Zapata, P.J., and M. Serrano. 2004. Polyamines and ethylene changes during germination of different plant species under salinity. Plant Sci. 167: 781-788. | ||
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