Selection of Proper Sturgeon Breeders Based on Genetic Identification | ||
| ماهیان خاویاری | ||
| Article 3, Volume 2, Issue 98, March 2020, Pages 25-35 PDF (1.02 M) | ||
| Document Type: Tarvij | ||
| Authors | ||
| Mahtab Yarmohammadi* ; rezvan kazemi; Ali halajian; Ayoub Yousefi; Mohamad ali yazdani; mohamad hasanzade saber; Ali Hosseinpour | ||
| International Sturgeon Research Institute, Agricultural Research Education, Extension Organization | ||
| Abstract | ||
| Long maturity in sturgeon is the most important obstacle to their breeding. Due to the large decline of natural populations of these fishes in the Caspian Sea, the continuation of production and aquaculture of sturgeon requires efficient production management based on their rearing. It is important to know the genetic structure of breeding populations in order maintain the genetic diversity of breeders. In recent years, in addition to breeding management, genetic management of breeders is of particular importance. Therefore, it is necessary to prepare a genetic certificate and then to study their genetic diversity before breeding. Using microsatellite genetic marker is one of the most efficient methods for studying genetic diversity in aquaculture species. For this purpose, DNA was extracted after sampling the caudal fin in a population of sturgeon breeders and placing them in 96% alcohol. DNA samples are amplified using at least 4 microsatellite primers in species with high polymorphism and disomic inheritance patterns. The band pattern obtained using the primers used along with other morphological characteristics for each spawner is recorded in the database. Using the data obtained from amplified alleles and GenAlex software, genetic components were used to determine genetic diversity in the studied breeders including heterozygosity, allele number, Hardy-Weinberg equilibrium, and population differentiation index (Fst). If the genetic diversity in the population is appropriate and the alleles of the proper breeders are examined, breeding can be performed. The use of this method is crucial in the sustainable development of sturgeon aquaculture and the control of inbreeding, especially in commercial sturgeon aquaculture programs. | ||
| Keywords | ||
| Sturgeon; Aquaculture; Microsatellite; Genetic Identification | ||
| References | ||
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