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Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 76، شماره 2، شهریور 2021، صفحه 203-211 اصل مقاله (291.63 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2020.127122.1371 | ||
| نویسندگان | ||
| L Rahbarnia1؛ S Farajnia* 1، 2، 3؛ B Naghili1؛ N Saeedi4 | ||
| 1Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections. The present study aimed to compare the efficiency of conventional microbiological and serological approaches with nested Polymerase chain reaction (nested PCR) for rapid diagnosis of human brucellosis. A total of 120 subjects with symptoms of brucellosis were included in the study. The sensitivity and specificity of nested PCR for the detection of Brucella bacteria were compared with serological and blood culture methods. Out of 120 patients enrolled, brucellosis was detected in 73 (60.83%) cases based on serological tests with a blood culture confirmation in 8.33% of participants. Based on the obtained results, 55% of cases were positive in serum agglutination test (SAT≥1:160), and Coombs (C-SAT≥1:160) tests. Furthermore, seven negative SAT cases were positive in C-SAT as evidence of chronic brucellosis. The results of the 2-mercaptoethanol (2-ME) ≥ 1:80 test were negative in six SAT-positive cases. Based on nested PCR results, 68.18% and 56.06% SAT positive samples were also detected by blood nested PCR and serum nested PCR, respectively. The sensitivity of blood nested PCR was significantly more than serum nested PCR, SAT≥1:160, and blood culture (p <0.001). Moreover, the specificity of blood and serum nested PCR was obtained at 100%, compared to blood culture and SAT≥ 1:160. In the present study, the nested PCR was able to identify chronic brucellosis in SAT negative patients. As evidenced by the obtained results, the nested PCR showed higher efficiency for rapid diagnosis of human brucellosis, as compared to the blood culture method. Furthermore, the findings pointed to the high performance of nested PCR for rapid diagnosis of both chronic and acute brucellosis. | ||
| کلیدواژه‌ها | ||
| Brucellosis؛ C-SAT؛ 2ME؛ Nested PCR؛ SAT | ||
| عنوان مقاله [English] | ||
| ارزیابی مقایسه ای روش nested PCR برای تشخیص سریع بروسلوز انسانی | ||
| چکیده [English] | ||
| بروسلوز به عنوان یک بیماری زونوتیک با درصد مرگ و میر بالا در صورت عدم درمان می باشد. تشخیص اولیه بروسلوز جهت جلوگیری از بروز عونتهای مزمن ضروری می باشد.هذف از این مطالعه مقایسه کارایی روشهای سرولوژیکی و میکروبی مرسوم با روش مولکولی nested PCR جهت تشخیص سریع بروسلوز انسانی می باشد. مواد و روشها: در این مطالعه 120 نفر با نشانه های اولیه بروسلوز تحت نمونه گیری قرار گرفتند. حساسیت و دقت روش nested PCR با روش کشت خون و روش سرولوژیکی مورد مقایسه قرار گرفت. نتایج: از 120 نفر تحت مطالعه بیماری بروسلوز در 60.83% با روش سرولوژیکی شناسایی شد که تنها 8.33% آنها با روش کشت خون تایید گردید..در مجموع 55% موارد در دو تست وserum agglutination test (SAT≥1:160) Coombs (C-SAT≥1:160) مثبت گزارش شدند. علاوه براین 7 مورد در تست SAT منفی بودند که در تست C-SAT مثبت گزارش شدند که نشاندهنده بروز بروسلوز مزمن بودند. نتایج تست 2-mercaptoethanol (2-ME) ≥ 1:80 تنها در 6 مورد از نمونه های مثبت SAT منفی بود. براساس نتایج nested PCR ، 68.18% نمونه های مثبت SAT به وسیله blood nested PCR مثبت گزارش شدند و 56.06% توسط روش serum nested PCR. حساسیت blood nested PCR به طور قابل توجهی با P بحث:براساس یافته های ما ، nested PCR کارایی بالاتری را نسبت به کشت خون برای تشخیص سریع بروسلوز انسانی دارد.نتایج نشاندهنده کارایی بالای nested PCR جهت شناسایی سریع بروسلوز مزمن و حاد دارد. | ||
| کلیدواژه‌ها [English] | ||
| بروسلوز-SAT, C-SAT, 2ME, Nested PCR | ||
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