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Molecular Detection of Theileria annulata among Dairy Cattle and Vector Ticks in the Herat Area, Afghanistan | ||
| Archives of Razi Institute | ||
| مقاله 11، دوره 76، شماره 1، خرداد 2021، صفحه 79-85 اصل مقاله (197.66 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.128212.1407 | ||
| نویسندگان | ||
| M Samiurahman Amiri؛ S Yaghfoori؛ Gh Razmi* | ||
| Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Theileriosis is one of the most important diseases in tropical and subtropical regions and leads to annual economic losses, such as the reduction of dairy products and casualties. Although the clinical form of bovine theileriosis has been observed in Afghanistan, to the best of our knowledge, no comprehensive study has been conducted on this issue. This molecular survey was performed to identify Theileria annulata and tick vectors in dairy cattle in the Herat area, Afghanistan, from June 2015-September 2016. A total of 100 dairy cattle were clinically examined and their blood smears, EDTA blood samples, and ixodid ticks were collected. The blood samples were transported to the laboratory, followed by the preparation of the blood smears and staining with the Giemsa method. The collected ticks were identified at the species (spp) level using the identification key and were then separated into 70 tick pools according to their species. Subsequently, the salivary glands were dissected out in 0.85% saline under a stereomicroscope. The DNA of blood and salivary glands was extracted using a commercial kit and analyzed by polymerase chain reaction (PCR). The ring form of Theileria spp infection was observed in 22 (22%) of blood smears, while 74% of blood samples were T. annulata positive using PCR. Among the collected ticks, the numbers of male and female ticks were obtained at 219 and 130 ticks, respectively. The frequency of tick spp was rated in descending order as Hyalomma annatolicum (73.9%), Hyalomma excavatum (22.3%), Hyalomma nymph spp (12%), Hyalomma marginatum (1.7%), Hyalomma asiaticum (1.1%), and Hyalomma rufipes (0.75%). The PCR results showed that seven pools belonging to salivary glands of H. anatolicum were infected with T. annulata. Based on the obtained results, it can be concluded that T. annulata had a high frequency in dairy cattle and H. anatoloicum was also identified, such as the vectors of T. annulata in the Herat area, Afghanistan. | ||
| کلیدواژهها | ||
| Theileria annulata؛ PCR؛ Cattle؛ Ixodid tick؛ Afghanistan | ||
| عنوان مقاله [English] | ||
| شناسایی ملکولی تیلریا آنولاتا در گاوهای شیری و کنه های ناقل شهر هرات افغانستان | ||
| چکیده [English] | ||
| تیلریوزیس یکی از بیماری های مهم مناطق گرمسیری و نیمه گرمسیری است و سبب خسارات اقتصادی بالا ناشی از کاهش مواد لبنی و مرگ ومیر حیوانات می شود. با وجود مشاهده علائم بالینی تیلریوزیس گاوی در کشور افغانستان تاکنون هیچگونه مطالعه جامعی در این باره انجام نشده است. بهمین دلیل از خرداد 1394 تا شهریور 1395، بررسی مولکولی برای شناسایی تیلریا انولاتا و کنه های ناقل درشهر هرات ، افغانستان انجام شد. تعداد 100 گاو شیری در این مدت مورد معاینه بالینی قرار گرفتند و سپس گسترش نازک خون، نمونه های خون حاوی EDTA به همراه کنه سخت جمع آوری شد. گسترش های تهیه شده با گیمسا رنگ آمیزی شدند. گونه های کنه های جمع آوری شده با استفاده از کلید تشخیص شناسایی و به 70 مجموعه پنج تایی برحسب گونه تقسیم شدند. بااستفاده از لوپ و وسایل حشره شناسی غدد بزاقی کنه ها جدا سازی شدند. DNA غدد بزاقی کنه ها به همراه نمونه های خون با استفاده ازکیت تجاری استخراج گردید و با روش PCR مورد آزمایش قرار گرفتند. در بررسی میکروسکوپی فرم حلقوی تیلریا در 22% از گسترش های خونی مشاهده شد. در حالیکه در آزمایش PCR، 74% خون های جمع آوری شده از نظر آلودگی به تیلریا آنولاتا مثبت بودند. کنه ها جمع آوری شده شامل 219 کنه نر و 130 کنه ماده بودند. فراوانی کنه های جمع اوری شده به ترتیب هیالوما آناتولیکم 9/73% ، هیالوما اکسکاواتوم 3/22% ، هیالوما آسیاتیکوم 1/1% هیالو ما مارژیناتوم 7/1% و هیالو ما روفیپس 57/0 %و نوچه کنه هیالوما 12 % بودند. نتایج آزمایش PCR ، آلودگی به تیلریا آنولاتا را در هفت مجموعه غدد بزاقی کنه هیالوما آناتولیکم نشان داد. براساس نتایج بدست آمده، فراوانی بالای آلودگی تیلریا آنولاتا در گاو های شیری شهر هرات افغانستان بدست آمد و هیالوما آناتولیکم به عنوان ناقل این بیماری شناسایی شد. | ||
| کلیدواژهها [English] | ||
| تیلریا آنولاتا, PCR, گاو, کنه های سخت, افغانستان | ||
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