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Composition and Anti-Toxicity Effects of Cichorium intybus Distillate on Serum Antioxidant Status in Carbon Tetrachloride-Treated Rats | ||
| Archives of Razi Institute | ||
| مقاله 14، دوره 76، شماره 1، خرداد 2021، صفحه 107-117 اصل مقاله (545.04 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.127303.1378 | ||
| نویسندگان | ||
| A Seghatoleslam1؛ Z Khoshdel1؛ R Ghafouri1؛ Sh Fakher1؛ M Molaei1؛ M Namavari2؛ F Zal* 1 | ||
| 1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran | ||
| چکیده | ||
| The role of oxidative stress in female fertility is a compelling area for research. According to traditional medicine, Cichorium intybus, known as Kasni, is believed to improve fertility. For this purpose, the effects of C. intybus distillate (CI) on blood antioxidant status were assessed in rats with carbon tetrachloride (CCl4)-induced toxicity. The rats were assigned to four experimental groups of Control, CI, CCl4, and CI+CCl410 (n=10 in each group). The level of antioxidant enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT), as well as lipid peroxidation and reduced glutathione (GSH) level, were measured in serum samples. In the second part of the study, the antioxidant activity and phytochemical composition of the hydrodistillate of C. intybus aerial parts were determined by DPPH radical scavenging and gas chromatography-mass spectrometry analysis, respectively. The administration of CCl4 decreased the enzyme activities of GPx, GR, and CAT which were significantly ameliorated after CI administration. The decreased level of serum GSH following CCl4 administration was not considerably elevated in the CI+CCl4 group. Furthermore, the level of malondialdehyde in the serum of CI+CCl4 rats was decreased, compared to the CCl4 group. The main compositions of the essential oil from the C. intybus distillate were the antioxidants of Pulegone (8.10%), Piperitenone (7.68%), dihydroactinidiolide (5.0%), and carvone (4.18%). The antioxidant activity of the distillate was obtained at 75µg/l using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) test. In general, the results of the present study demonstrated that C. intybus distillate, as a safe herbal remedy, can attenuate CCl4-induced oxidative damages via boosting the endogenous antioxidant defense system. | ||
| کلیدواژهها | ||
| Chicory؛ Catalase؛ Glutathione؛ Malondialdehyde؛ Mass spectrometry | ||
| عنوان مقاله [English] | ||
| ترکیب شیمیایی و اثرات سم زدایی عرق کاسنی بر وضعیت آنتی اکسیدان سرم در موشهای صحرایی ماده تیمار شده با تتراکلرید کربن | ||
| چکیده [English] | ||
| نقش استرس اکسیداتیو در باروری جنس ماده موضوعی قابل توجه برای پژوهش است. در طب سنتی تصور بر این است که گیاه کاسنی موجب تقویت باروری می گردد. در همین راستا اثرات عرق کاسنی بر وضعیت آنتی اکسیدان سرم موشهای صحرایی سمی شده توسط تترا کلرید کربن مورد بررسی قرار گرفت. موشهای صحرایی به چهار گروه آزمایشی ده تایی تقسیم شدند: کنترل، CI، CCl4 و CI+CCl4. سطح فعالیت آنزیمهای آنتی اکسیدان از جمله گلوتاتیون پراکسیداز (GPx)، گلوتاتیون ردوکتاز و کاتالاز و همچنین سطح پراکسیداسیون چربیها و گلوتاتیون احیا (GSH) در نمونه های سرم اندازه گیری شد. در بخش دوم مطالعه، فعالیت آنتی اکسیدان و ترکیب فیتوشیمیایی عرق قسمتهای هوایی کاسنی توسط آنالیز رادیکال خواری DPPH و اسپکترومتری GC-MS اندازه گیری شد. تجویز تتراکلریدکربن فعالیت آنزیمی GPx، GR و کاتالاز را کاهش داد که با تجویز عرق کاسنی بهبود پیدا کرد. در گروه CI+CCl4 سطح کاهش یافته GSH پس از تجویز تتراکلریدکربن افزایش چشمگیری نداشت. بعلاوه سطح MDA در سرم موشهای صحرایی گروه CI+CCl4 در مقایسه با گروه تتراکلریدکربن کاهش یافت. ترکیبات اصلی روغن عصاره عرق کاسنی، آنتی اکسیدانهای Pulegone (%1/8)، Piperitenone (%68/7)، dihydroactinidiolide (%5) و carvone (%18/4) هستند. فعالیت آنتی اکسیدانی عرق بر اساس تست DPPH معادل µg /l75 اندازه گیری شد. در مجموع نتایج ما نشان داد که عرق کاسنی به عنوان یک درمان گیاهی ایمن می تواند آسیبهای اکسیداتیو القا شده با تتراکلریدکربن را احتمالا از طریق تقویت سیستم دفاعی آنتی اکسیدانی داخلی کاهش دهد. | ||
| کلیدواژهها [English] | ||
| کاسنی, کاتالاز, گلوتاتیون, مالون دی آلدئید, اسپکترومتری | ||
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