| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,230 |
| تعداد مقالات | 35,788 |
| تعداد مشاهده مقاله | 54,014,564 |
| تعداد دریافت فایل اصل مقاله | 93,171,953 |
Composition and Anti-Toxicity Effects of Cichorium intybus Distillate on Serum Antioxidant Status in Carbon Tetrachloride-Treated Rats | ||
| Archives of Razi Institute | ||
| مقاله 14، دوره 76، شماره 1، خرداد 2021، صفحه 107-117 اصل مقاله (545.04 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.127303.1378 | ||
| نویسندگان | ||
| A Seghatoleslam1؛ Z Khoshdel1؛ R Ghafouri1؛ Sh Fakher1؛ M Molaei1؛ M Namavari2؛ F Zal* 1 | ||
| 1Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran | ||
| چکیده | ||
| The role of oxidative stress in female fertility is a compelling area for research. According to traditional medicine, Cichorium intybus, known as Kasni, is believed to improve fertility. For this purpose, the effects of C. intybus distillate (CI) on blood antioxidant status were assessed in rats with carbon tetrachloride (CCl4)-induced toxicity. The rats were assigned to four experimental groups of Control, CI, CCl4, and CI+CCl410 (n=10 in each group). The level of antioxidant enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR), and catalase (CAT), as well as lipid peroxidation and reduced glutathione (GSH) level, were measured in serum samples. In the second part of the study, the antioxidant activity and phytochemical composition of the hydrodistillate of C. intybus aerial parts were determined by DPPH radical scavenging and gas chromatography-mass spectrometry analysis, respectively. The administration of CCl4 decreased the enzyme activities of GPx, GR, and CAT which were significantly ameliorated after CI administration. The decreased level of serum GSH following CCl4 administration was not considerably elevated in the CI+CCl4 group. Furthermore, the level of malondialdehyde in the serum of CI+CCl4 rats was decreased, compared to the CCl4 group. The main compositions of the essential oil from the C. intybus distillate were the antioxidants of Pulegone (8.10%), Piperitenone (7.68%), dihydroactinidiolide (5.0%), and carvone (4.18%). The antioxidant activity of the distillate was obtained at 75µg/l using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) test. In general, the results of the present study demonstrated that C. intybus distillate, as a safe herbal remedy, can attenuate CCl4-induced oxidative damages via boosting the endogenous antioxidant defense system. | ||
| کلیدواژهها | ||
| Chicory؛ Catalase؛ Glutathione؛ Malondialdehyde؛ Mass spectrometry | ||
| عنوان مقاله [English] | ||
| ترکیب شیمیایی و اثرات سم زدایی عرق کاسنی بر وضعیت آنتی اکسیدان سرم در موشهای صحرایی ماده تیمار شده با تتراکلرید کربن | ||
| چکیده [English] | ||
| نقش استرس اکسیداتیو در باروری جنس ماده موضوعی قابل توجه برای پژوهش است. در طب سنتی تصور بر این است که گیاه کاسنی موجب تقویت باروری می گردد. در همین راستا اثرات عرق کاسنی بر وضعیت آنتی اکسیدان سرم موشهای صحرایی سمی شده توسط تترا کلرید کربن مورد بررسی قرار گرفت. موشهای صحرایی به چهار گروه آزمایشی ده تایی تقسیم شدند: کنترل، CI، CCl4 و CI+CCl4. سطح فعالیت آنزیمهای آنتی اکسیدان از جمله گلوتاتیون پراکسیداز (GPx)، گلوتاتیون ردوکتاز و کاتالاز و همچنین سطح پراکسیداسیون چربیها و گلوتاتیون احیا (GSH) در نمونه های سرم اندازه گیری شد. در بخش دوم مطالعه، فعالیت آنتی اکسیدان و ترکیب فیتوشیمیایی عرق قسمتهای هوایی کاسنی توسط آنالیز رادیکال خواری DPPH و اسپکترومتری GC-MS اندازه گیری شد. تجویز تتراکلریدکربن فعالیت آنزیمی GPx، GR و کاتالاز را کاهش داد که با تجویز عرق کاسنی بهبود پیدا کرد. در گروه CI+CCl4 سطح کاهش یافته GSH پس از تجویز تتراکلریدکربن افزایش چشمگیری نداشت. بعلاوه سطح MDA در سرم موشهای صحرایی گروه CI+CCl4 در مقایسه با گروه تتراکلریدکربن کاهش یافت. ترکیبات اصلی روغن عصاره عرق کاسنی، آنتی اکسیدانهای Pulegone (%1/8)، Piperitenone (%68/7)، dihydroactinidiolide (%5) و carvone (%18/4) هستند. فعالیت آنتی اکسیدانی عرق بر اساس تست DPPH معادل µg /l75 اندازه گیری شد. در مجموع نتایج ما نشان داد که عرق کاسنی به عنوان یک درمان گیاهی ایمن می تواند آسیبهای اکسیداتیو القا شده با تتراکلریدکربن را احتمالا از طریق تقویت سیستم دفاعی آنتی اکسیدانی داخلی کاهش دهد. | ||
| کلیدواژهها [English] | ||
| کاسنی, کاتالاز, گلوتاتیون, مالون دی آلدئید, اسپکترومتری | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Ahangarpour, A., Oroojan, A.A., Heidari, H., Ghaedi, E., Taherkhani, R., 2014. Effects of Hydro-alcoholic Extract from Arctium lappa L.(Burdock) Root on Gonadotropins, Testosterone, and Sperm Count and Viability in Male Mice with Nicotinamide/Streptozotocin-Induced Type 2 Diabetes. Malays J Med Sci 22, 25-32.
Al-Rasheed, N.M., Fadda, L.M., Al-Rasheed, N.M., Ali, H.M., Yacoub, H.I., 2016. Down-regulation of NFKB, Bax, TGF-β, Smad-2mRNA expression in the livers of carbon tetrachloride treated rats using different natural antioxidants. Braz Arch Biol Technol 59.
Asirvatham, R., Usha, J.J., 2017. Evaluation of In vitro and In vivo Antioxidant potential of Morinda reticulata Gamble Tubers in Wistar Albino Rats Subjected to CCl4 and Paracetamol induced Hepatotoxicity. Indones J Pharm 28, 147.
Būdienė, A.J.J., 2008. Volatile constituents from aerial parts and roots of Cichorium intybus L. (chicory) grown in Lithuania. chemija 19, 25–28.
Chadwick, M., Trewin, H., Gawthrop, F., Wagstaff, C., 2013. Sesquiterpenoids lactones: benefits to plants and people. Int J Mol Sci 14, 12780-12805.
Darché, R.L., Ruder, E.H., Blumberg, J., Hartman, T.J., Goldman, M.B., 2017. Antioxidants in Reproductive Health and Fertility. Nutritional Antioxidant Therapies: Treatments and Perspectives, Springer, pp. 113-136.
Das, S., Vasudeva, N., Sharma, S., 2016. Cichorium intybus: A concise report on its ethnomedicinal, botanical, and phytopharmacological aspects. Drug Discov Ther 7, 1-12.
Gohari, A., Noorafshan, A., Akmali, M., Zamani-Garmsiri, F., Seghatoleslam, A., 2017. Urtica Dioica Distillate (Aragh Gazaneh) Regenerates Pancreatic Beta Cells in Streptozotocin-Induced Diabetic Rats. Iran J Med Sci 41, 174-183.
Gol, N.R.N., R. Z. ; Chamsaz, M., 2014. A comparative study of the chemical composition and antioxidant activities of roots, seeds and aerial parts of chicory (Cichorium intybus L.). Int J Biolsci 5, 250-257.
Gupta, S., Agarwal, A., Krajcir, N., Alvarez, J.G., 2006. Role of oxidative stress in endometriosis. Reprod Biomed Online 13, 126-134.
Gupta, V.K., Gupta, M., Sharma, S.K., 2011. Evaluation of antioxidant potential of Ficus religiosa (Linn.) roots against carbon tetrachloride-induced liver injury. J Med Plant Res 5, 1582-1588.
H Sekhon, L., Gupta, S., Kim, Y., Agarwal, A., 2010. Female infertility and antioxidants. Curr Womens Health Rev 6, 84-95.
Haghi, Arshi, G., Ghazian, R., Hosseini, F., 2012. Chemical Composition of Essential Oil of Aerial Parts of Cichorium intybus L. J Essent Oil Bear Pl 15, 213-216.
Kuriakose, J., Raisa, H.L., Vysakh, A., Eldhose, B., Latha, M., 2017. Terminalia bellirica (Gaertn.) Roxb. fruit mitigates CCl4 induced oxidative stress and hepatotoxicity in rats. Biomed Pharmacother 93, 327-333.
Mashhoody, T., Rastegar, K., Zal, F., 2014. Perindopril may improve the hippocampal reduced glutathione content in rats. Adv Pharm Bull 4, 155-159.
Moon, J.K., Shibamoto, T., 2009. Antioxidant assays for plant and food components. J Agric Food Chem 57, 1655-1666.
Muhammad, A., Muhammad, S., Zahid, M., Shazia, A.B., Mir Munsif, A.T., 2014. Antimicrobial Activity of Extract and Fractions of Different Parts and GC-MS Profiling of Essential Oil of Cichorium intybus Extracted by Super Critical Fluid Extraction. Asian J Chem. 26, 531-536.
Mulla, A.A., Fazari, A.B., Elkhouly, M., Moghaddam, N., 2018. Role of Antioxidants in Female Fertility. Open J Obstet Gynecol 8, 85-91.
Rashidi, M., Seghatoleslam, A., Namavari, M., Amiri, A., Fahmidehkar, M.A., Ramezani, A., et al., 2017. Selective Cytotoxicity and apoptosis-induction of Cyrtopodion scabrum extract against digestive cancer cell lines. Int J Cancer Manag 10.
Saric-Kundalic, B., Dobes, C., Klatte-Asselmeyer, V., Saukel, J., 2011. Ethnobotanical survey of traditionally used plants in human therapy of east, north and north-east Bosnia and Herzegovina. J Ethnopharmacol 133, 1051-1076.
Seghatoleslam, A., Mashkour, N., Namavari, M., Azarmehr, B., Nejabat, M., 2014. The potential effects of herbal distillates with hot and cold temperament on cell metabolic activity and growth: a preliminary in vitro study. J Pharmaceutical Biomedical Sci 4, 532-535.
Sharma, V., Agrawal, R., 2017. In vivo antioxidant and hepatoprotective potential of Glycyrrhiza glabra extract on carbon tetra chloride (CCl4) induced oxidative-stress mediated hepatotoxicity. Int J Res Med Sci 2, 314-320.
Showell, M.G., Mackenzie‐Proctor, R., Jordan, V., Hart, R.J., 2017. Antioxidants for female subfertility. The Cochrane Library.
Sobeh, M., Youssef, F.S., Esmat, A., Petruk, G., El-Khatib, A.H., Monti, D.M., et al., 2018. High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage. Food Chem Toxicol 113, 145-153.
Street, R.A., Sidana, J., Prinsloo, G., 2013. Cichorium intybus: Traditional uses, phytochemistry, pharmacology, and toxicology. Evid Based Complement Alternat Med 15, 579319.
Yarahmadi, A., Zal, F., Bolouki, A., 2017. Protective effects of quercetin on nicotine induced oxidative stress in 'HepG2 cells'. Toxicol Mech Methods 27, 609-614.
Zahid Khorshid Abbas a, *, Shalini Saggu a,1, Mohamed I. Sakeran b,c,, Nahla Zidan d, e., Hasibur Rehman a, Abid A. Ansari a, 2015. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves. Saudi J Biol Sci 22, 322-326.
Zal, F., Khademi, F., Taheri, R., Mostafavi-Pour, Z., 2018. Antioxidant ameliorating effects against H2O2-induced cytotoxicity in primary endometrial cells. Toxicol Mech Methods 28, 122-129.
Zal, F., Mahdian, Z., Zare, R., Soghra, B., Mostafavi-Pour, Z., 2014. Combination of vitamin E and folic acid ameliorate oxidative stress and apoptosis in diabetic rat uterus. Int J Vitam Nutr Res 84, 55-64. | ||
|
آمار تعداد مشاهده مقاله: 2,696 تعداد دریافت فایل اصل مقاله: 3,251 |
||
