| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,168 |
| تعداد مقالات | 35,197 |
| تعداد مشاهده مقاله | 50,932,196 |
| تعداد دریافت فایل اصل مقاله | 90,937,591 |
Evaluation of Heating and Irradiation Methods for Production of Purified Protein Derivative (PPD) of Mycobacterium Tuberculosis | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 75، شماره 4، اسفند 2020، صفحه 439-449 اصل مقاله (587.63 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.123082.1238 | ||
| نویسندگان | ||
| N. Mosavari1؛ A. Karimi2؛ K. Tadayon1؛ Gh. Shahhosseini* 3؛ A. Zavaran Hosseini4؛ M. Babaie1 | ||
| 1Reference Laboratory of Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 2Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran | ||
| 4Animal Science Research Institute of Iran, Alborz, Iran | ||
| چکیده | ||
| Tuberculin skin test, also known as the tuberculin or purified protein derivative (PPD) test, is an extensively applied diagnostic test for the detection of primary infection with Mycobacterium tuberculosis (Mtb). The production of PPD is accompanied by some difficulties that require a series of modifications in the production and purification processes. The present study aimed to determine the facilitation level of the manufacturing process by modifying evaluation methods for the production of PPD tuberculin. Mtb strains were cultured in Lowenstein-Jensen media, and the cultured strains were inoculated into the Dorset-Henley liquid medium by the biphasic medium of potato-Dorset-Henley. After incubation, flasks containing cultured strain were selected for bacterial inactivation, and the optimal gamma radiation dose(s) was determined. Tuberculoproteins were precipitated by ammonium sulfate (AS) and Trichloroacetic acid (TCA). Protein concentration was determined using the Bradford and Kjeldahl protein assay methods. Finally, the lymphocyte transformation test and potency test were performed. Based on the results, the Dorset-Henley liquid medium is suitable for the massive growth of the bacterium. The transferal of Mtb from solid to liquid medium was directly carried out without intermediate culture. It was found that during tuberculoprotein production, heating at 100°C for 3 h would be safe for killing mycobacterium. Furthermore, the simultaneous use of heating and gamma irradiation (8 kGgy) killed all of the mycobacteria, while doses of 1, 1.5, and 7 kGy decreased a significant number of bacterial cells. The results also indicated that the concentration of tuberculoprotein extracted by TCA precipitation method was higher than that obtained by AS precipitation. The tuberculoproteins which were produced by these two methods in the lymphocyte transformation test were not significantly different in terms of potency (P>0.05). Moreover, due to the high volume of produced protein, the protein measurement was more efficiently carried out by the Kjeldahl method, compared to the Bradford method. Finally, the results of the present study demonstrated that in addition to the novel approach of gamma irradiation, optimum methods are efficient and applicable in the production of PPD tuberculin. | ||
| کلیدواژهها | ||
| Mycobacterium tuberculosis؛ PPD؛ Gamma radiation؛ Lymphocyte transformation test | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Abou-Zeid, C., Smith, I., Grange, J.M., Ratliff, T.L., Steele, J., Rook, G.A., 1988. The secreted antigens of Mycobacterium tuberculosis and their relationship to those recognized by the available antibodies. Microbiology 134, 531-538.
Babaie, M., Ghaem panah, A., Mehrabi, Z., Mollaei, A., 2020. Partial purification and characterization of antimicrobial effects from snake (Echis carinatus), scorpion (Mesosobuthus epues) and bee (Apis mellifera) venoms. Iran J Med Microbiol 14(5), 460-477.
Bendinelli, M., Friedman, H., 1988. Mycobacterium Tuberculosis: Interactions with the Immune System, Springer Science & Business Media.
Capsel, R.T., 2015. Development of an improved production method, determination of protein composition, and potency characterization of Mycobacterium avium subsp. paratuberculosis purified protein derivative. Corbett, E.L., Watt, C.J., Walker, N., Maher, D., Williams, B.G., Raviglione, M.C., et al., 2003. The growing burden of tuberculosis: global trends and interactions with the HIV epidemic. Arch Intern Med 163, 1009-1021.
Demicheli, M.C., Goes, A.M., de Andrade, A.S., 2007. Ultrastructural changes in Paracoccidioides brasiliensis yeast cells attenuated by gamma irradiation. Mycoses 50, 397-402.
Dye, C., Scheele, S., Dolin, P., Pathania, V., Raviglione, M.C., 1999. Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project. Jama 282, 677-686.
Fogel, N., 2015. Tuberculosis: A disease without boundaries. Tuberculosis 95, 527-531.
Garcia, M.M., Brooks, B.W., Stewart, R.B., Dion, W., Trudel, J.R., Ouwerkerk, T., 1987. Evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents. Can J Vet Res 51, 285-289.
Hassan Zadeh, J., Nasehi, M., Rezaianzadeh, A., Tabatabaee, H., Rajaeifard, A., Ghaderi, E., 2013. Pattern of reported tuberculosis cases in iran 2009-2010. Iran J Public Health 42, 72-78.
Horwitz, W., Latimer, G.W., 2005. Official methods of analysis of AOAC International, AOAC International, Gaithersburg, Md.
ICMSF, 1980. 3-Ionizing Irradiation Microbial Ecology of Foods, Academic Press, pp. 46-69.
Khazaei, H.A., Rezaei, N., Bagheri, G.R., Dankoub, M.A., Shahryari, K., Tahai, A., et al., 2005. Epidemiology of tuberculosis in the Southeastern Iran. Eur J Epidemiol 20, 879-883.
Kiran, U., Kumar, R., Sharma, A., 1985. Efficacy of three mycobacterial antigens in the serodiagnosis of tuberculosis. European journal of respiratory diseases 66, 187-195. Eur J Respir Dis.
Marcdante, K., Kliegman, R.M., 2014. Nelson Essentials of Pediatrics E-Book, Elsevier Health Sciences.
Prevention, C.f.D.C.a., 2016. TB Elimination Tuberculin Skin Testing. https://www.cdc.gov/tb.
Rezwan, M., Lanéelle, M.-A., Sander, P., Daffé, M., 2007. Breaking down the wall: Fractionation of mycobacteria. J Microbiol Methods 68, 32-39.
Santema, W., Overdijk, M., Barends, J., Krijgsveld, J., Rutten, V., Koets, A., 2009. Searching for proteins of
Mycobacterium avium subspecies paratuberculosis with diagnostic potential by comparative qualitative proteomic analysis of mycobacterial tuberculins. Vet Microbiol 138, 191-196.
Shahhosseini, G., Karimi, A., Amanpour, S., Mansouri, M., 2018. Effect of gamma irradiation on microbial decontamination and crude nutrient content and mineral nutrient composition of laboratory animal diets. Arch Razi Inst 74, 175-182.
Shaltout, F., Em, R., Ahmed, T., Asmaa, A., 2017. Studying the Effect of Gamma Irradiation on Bovine Offal's Infected with Mycobacterium tuberculosis Bovine Type. J Food Biotechnol Res 1, 1-5.
Sterling, T.R., Lehmann, H.P., Frieden, T.R., 2003. Impact of DOTS compared with DOTS-plus on multidrug resistant tuberculosis and tuberculosis deaths: decision analysis. BMJ (Clinical research ed.) 326, 574.
Wales, A., Kusel, J., 1992. Biochemistry of irradiated parasite vaccines: suggested models for their mode of action. Parasitol Today 8, 358-363.
WHO, 2018. Tuberculosis fact sheet. http://www.who.int/en/news-room/fact-sheets/detail/tuberculosis.
Wynne, J.W., Shiell, B.J., Colgrave, M.L., Vaughan, J.A., Beddome, G., Michalski, W.P., 2012. Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis. Proteome Sci 10, 22.
Zack, M.B., Stottmeier, K., Berg, G., Kazemi, H., 1974. The effect of radiation on microbiologic characteristics of M tuberculosis. Chest 66, 240-243. | ||
|
آمار تعداد مشاهده مقاله: 16,321 تعداد دریافت فایل اصل مقاله: 66,301 |
||
