| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,530,566 |
| تعداد دریافت فایل اصل مقاله | 90,479,236 |
Hemagglutinin-neuraminidase Sequence and Phylogenetic Analysis of Two Newcastle Disease Virus Isolated from Chickens in Iran | ||
| Archives of Razi Institute | ||
| مقاله 6، دوره 76، شماره 1، خرداد 2021، صفحه 31-39 اصل مقاله (225.21 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.124844.1289 | ||
| نویسندگان | ||
| M. H Kiani1؛ M. H Bozorgmehrifard* 1؛ H Hosseini2؛ S Charkhkar1؛ A Ghalyanchilangeroudi3 | ||
| 1Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran | ||
| 3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Newcastle disease is a highly contagious viral infection affecting many species of birds that can spread fast between poultry houses and cause a heavy economic burden on the poultry industry all around the world. Fusion and hemagglutinin-neuraminidase (HN) protein are important in the pathogenesis of the Newcastle disease virus (NDV). The HN protein is a critical viral protein with multiple functions and plays a key role in the formation of the virulence of NDV. Head of HN protein is responsible for receptor binding, neuraminidase activity. This study aimed to investigate the sequence homology of hemagglutinin-neuraminidase of two NDV isolates sampled from infected farms in Iran. The samples were collected from flocks that had been vaccinated by both types of live and killed vaccines for NDV. After isolation of NDV, the viruses were subjected to the polymerase chain reaction (PCR) amplification using two pairs of specific primers designed for the HN gene to amplify the complete HN gene (1730bp). Afterward, the PCR products were sequenced and analyzed by phylogenetic tree construction software. Based on the analysis, substantial sequence homology among Iranian isolates is within the range of 97.1-100%. Moreover, the sequence homology searching revealed a level of similarity between HN sequences of Iranian isolates and the HN sequences from other countries, particularly Asian ones. For instance, a high homology ratio (95.34%) was found between Iranian isolates and the sequences registered on online molecular databases from China. Based on phylogenetic analysis, the NDV isolates belong to the VIId genotype. Finally, it can be concluded that monitoring the circulation of NDVs among poultry and other birds can help to obtain an insight into the evolution of NDVs and control of panzootic viruses in the future. | ||
| کلیدواژهها | ||
| Hemagglutinin-neuraminidase؛ Iran؛ Newcastle Disease Virus؛ Phylogenetic tree | ||
| عنوان مقاله [English] | ||
| تعیین توالی و آنالیز فیلوژنی ژن هماگلوتینین و نورآمینیداز دو ویروس نیوکاسل جدا شده از ماکیان در ایران | ||
| مراجع | ||
|
Ahmadi, E., Pourbakhsh, S.A., Ahmadi, M., Talebi, A., 2014. Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran. Turk J Vet Anim Sci 38, 383-387.
Cao, Y., Gu, M., Zhang, X., Liu, W., Liu, X., 2013. Complete Genome Sequences of Two Newcastle Disease Virus Strains of Genotype VIII. Genome Announc 1.
Diel, D.G., da Silva, L.H., Liu, H., Wang, Z., Miller, P.J., Afonso, C.L., 2012. Genetic diversity of avian paramyxovirus type 1: proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. MEEGID 12, 1770-1779.
Ebrahimi, M.M., Shahsavandi, S., Moazenijula, G., Shamsara, M., 2012. Phylogeny and evolution of Newcastle disease virus genotypes isolated in Asia during 2008–2011. Virus Genes 45, 63-68. Esmaelizad, M., Ashtiani, M.P., NIARAKI, S.J., Hashemnejad, K., 2012. Identification of 23 specific nucleotide patterns in the HN gene of Newcastle disease viruses isolated from Iran. Turk J Biol 36, 135-142.
Firouzamandi, M., Moeini, H., Hosseini, D., Bejo, M.H., Omar, A.R., Mehrbod, P., et al., 2016. Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes. J Vet Sci 17, 21-26.
Ghalyanchilangeroudi, A., Hosseini, H., Jabbarifakhr, M., Fallah Mehrabadi, M.H., Najafi, H., Ghafouri, S.A., et al., 2018. Emergence of a virulent genotype VIIi of Newcastle disease virus in Iran. Avian Pathol 47, 509-519.
Hosseini, H., Ghalyanchi-Langeroudi, A., Torabi, R., 2014. Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2010-2012. Avian Dis 3, 373-376.
Hu, S., Wang, T., Liu, Y., Meng, C., Wang, X., Wu, Y., et al., 2010. Identification of a variable epitope on the Newcastle disease virus hemagglutinin-neuraminidase protein. Vet Microbiol 140, 92-97.
Kiani, M.H., Bozorgmehrifard, M.H., Hosseini, H., Charkhkar, S., Ghalyanchi, A., 2016. Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2014–2015. Iran J Virol 10, 53-57.
Kumar, S., Stecher, G., Tamura, K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33, 1870-1874.
Manual, O.T., 2018. Newcaslte Disease.
Mehrabanpour, M.J., Khoobyar, S., Rahimian, A., Nazari, M.B., Keshtkar, M.R., 2014. Phylogenetic characterization of the fusion genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011. Veterinary research forum: an international quarterly journal. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, p. 187.
Munir, M., Cortey, M., Abbas, M., Afzal, F., Shabbir, M.Z., Khan, M.T., et al., 2012. Biological characterization and phylogenetic analysis of a novel genetic group of Newcastle disease virus isolated from outbreaks in commercial poultry and from backyard poultry flocks in Pakistan. Infect Genet Evol 12, 1010-1019.
Offeddu, V., Cowling, B.J., Peiris, J.M., 2016. Interventions in live poultry markets for the control of avian influenza: a systematic review. One Health 2, 55-64.
OIE, M., 2018. Manual of diagnostic tests and vaccines for terrestrial animals. Paris: Office International Des Epizooties.
Otim, M.O., Christensen, H., Jørgensen, P.H., Handberg, K.J., Bisgaard, M., 2004. Molecular characterization and phylogenetic study of Newcastle disease virus isolates from recent outbreaks in eastern Uganda. J Clin Microbiol 42, 2802-2805.
Sabouri, F., Vasfi Marandi, M., Bashashati, M., 2018. Characterization of a novel VIIl sub-genotype of Newcastle disease virus circulating in Iran. Avian Pathol 47, 90-99.
Samadi, S., Kianizadeh, M., Najafi, M.F., Nasab, S.D.M., Davatgar, A.M.H., Royaee, A., et al., 2014. Molecular characterization and phylogenetic study of velogenic Newcastle disease virus isolates in Iran. Virus Genes 48, 290-295.
Shahriari, A.G., Bagheri, A., Bassami, M.R., Malekzadeh Shafaroudi, S., Afsharifar, A.R., 2015. Cloning and expression of fusion (f) and haemagglutinin-neuraminidase (hn) epitopes in hairy roots of tobacco (nicotiana tabaccum) as a step toward developing a candidate recombinant vaccine against newcastle disease. J Cell Mol Res 7, 11-18. | ||
|
آمار تعداد مشاهده مقاله: 2,628 تعداد دریافت فایل اصل مقاله: 3,601 |
||