| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,208 |
| تعداد مقالات | 35,560 |
| تعداد مشاهده مقاله | 53,004,248 |
| تعداد دریافت فایل اصل مقاله | 92,288,878 |
بررسی ارتباط میان رشد Aspergillus flavus و تولید آفلاتوکسین هایB1 و B2 با ترکیبات فنولی و فلاونوئیدی پوست سبز و مغز ارقام پسته (مقاله انگلیسی) | ||
| آفات و بیماری های گیاهی | ||
| مقاله 14، دوره 87، شماره 2 - شماره پیاپی 109، اسفند 1398، صفحه 13-23 اصل مقاله (596.01 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.126742.1288 | ||
| نویسندگان | ||
| مهدی محمدی مقدم1؛ سعید رضائی* 1؛ امیر حسین محمدی2؛ حمیدرضا زمانی زاده3؛ محمد مرادی4 | ||
| 1گروه گیاه پزشکی، دانشکده علوم کشاورزی و صنایع غذایی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران | ||
| 2پژوهشکده پسته، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، رفسنجان، ایران | ||
| 3هیئت علمی دانشگاه آزاد واحد علوم وتحقیقات تهران | ||
| 4پژوهشکده پسته، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| مسأله آلودگی پسته به قارچ Aspergillus flavus و آفلاتوکسین از تهدیدات مهم در عرصه تولید، صادرات و مصرف پسته در دنیا می باشد. در این تحقیق، ضمن تعیین میزان مقاومت ارقام پسته شاهپسند، عباسعلی، کله قوچی، خنجری، اکبری و پسته گرمه به آلودگی ناشی از قارچ A. flavus و آفلاتوکسین، ارتباط میان میزان ترکیبات فنولی و فلاونوئیدی پوست سبز و مغز این ارقام، به عنوان ترکیبات آنتی اکسیدانی، با میزان رشد قارچ A. flavus و تولید آفلاتوکسین مورد مطالعه قرار گرفت. مایه زنی مغز ارقام پسته با غلظت 6 10×2 اسپور در میلی لیتر از یک جدایه توکسین زای A. flavus صورت گرفته و غلظت آفلاتوکسین، ترکیبات فنولی و فلاونوئیدی مغز و پوست سبز به ترتیب به روش HPLC، فولین سکالتیو و رنگ سنجی با کلرید آلومینیوم تعیین گردید. نتایج نشان داد که ارقام پسته گرمه و اکبری دارای کمترین و رقم شاهپسند دارای بیشترین میزان کلنیزاسیون A. flavus و غلظت آفلاتوکسین بوده و به ترتیب به عنوان ارقام مقاوم و حساس به آلودگی ناشی از A. flavus تعیین شدند. سایر ارقام حدواسط این ارقام قرار گرفتند. میزان ترکیبات فنولی و فلاونوئیدی به طور معنی داری در ارقام مقاوم بیشتر از ارقام حساس بود. همچنین یک رابطه منفی معنی داری بین میزان ترکیبات فنولی و فلاونوئیدی پوست سبز و مغز پسته ارقام مختلف، با رشد قارچ A. flavus و تولید آفلاتوکسین در مغز این ارقام مشاهده گردید. این نتایج بیانگر نقش ترکیبات فنولی و فلاونوئیدی در مقاومت ارقام پسته به آلودگی ناشی از A. flavus و تولید آفلاتوکسین می باشد. | ||
| کلیدواژهها | ||
| مقاومت؛ آنتی اکسیدان ها؛ Aspergillus flavus | ||
| عنوان مقاله [English] | ||
| Relationship between Aspergillus flavus growth and aflatoxin B1 and B2 production with phenolic and flavonoid compounds in green hull and kernels of pistachio cultivars | ||
| نویسندگان [English] | ||
| Mehdi Mohammadi moghaddam1؛ Saeed Rezaee1؛ Amir Hossein Mohammadi2؛ Hamid reza zamanizadeh3؛ Mohammad Moradi4 | ||
| 1Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran | ||
| 3Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 4Pistachio Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran | ||
| چکیده [English] | ||
| Contamination to Aspergillus flavus and aflatoxin is a significant and chronic threat of pistachio production, consumption and exportation in the world. The aims of the present study was evaluation of resistance of pistachio cultivars (Shahpasand, Abbasali,Kale-ghouchi, Khanjari, Akbari and Pesteh-garmeh) and relationship between total phenolic content (TPC) and total flavonoid content (TFC) of green hull and kernels, as antioxidant, to A. flavus growth and aflatoxin production. Inoculation of pistachio kernels was done by 2×106 spores of toxigenic A.flavus and aflatoxins (B1 and B2), TPC and TFC of the green hull and kernels were measured by HPLC, Folin-Ciocalteu and aluminum chloride method, respectively. Results showed that Pesteh-garmeh and Akbari cultivars and Shahpasand with the lowest and highest percent colonization of A. flavus and aflatoxin production in kernels were respectively resistant and susceptible and other cultivars were intermediate. TPC and TFC were higher significantly in resistant cultivars than susceptible. A negative correlation was observed between TPC, TFC and aflatoxin content in kernel and green hull of pistachio cultivars, as well as TPC, TFC and A. flavus growth. These findings show the role of TPC and TFC in resistance of pistachio cultivars with contamination to A. flavus growth and aflatoxin production. | ||
| کلیدواژهها [English] | ||
| Resistance, Antioxidants, Aspergillus flavus | ||
| مراجع | ||
|
ABBAS, H.K. 2005. Aflatoxin and food safety. CRC Press, Taylor & Francis Group AHMADI, K., H.R. EBADZADEH, F. HATAMI, R. HOSSEINPOUR and H. ABDESHAH. 2017. Agricultural statistics (1396). Volume III: Horticultural products. Ministry of Agriculture-Jahad, Deputy of Planning and Economics, Information and Communication Technology Center, 233 p. ALLAMEH, A.A. and M. RAZZAGHI, 2002."Mycotoxins". first edition, Imam Hossein University Press. Tehran. 620pp. ARCAS, M.C., J.M. BOTIA, A.M. ORTUNO and J.A. DEL RIO. 2000. UV irradiation alters the levels of flavonoids involved in the defense mechanism of Citrus aurantium fruits against Peniillium digitatum. European Journal of Plant Pathology, 106: 617–622. ATANASOVA-PENICHON, V., C. BARREAU and F. RICHARD-FORGET. 2016. Antioxidant Secondary Metabolites in Cereals: Potential Involvement in Resistance to Fusarium and Mycotoxin Accumulation. Frontiers in microbiology, 566:1-16. BACKON, B., A.C. BILY, D. MELCION, B. CAHAGNIER, C. REGNAULT-ROGER, B.J.R. PHILOGE´NE and D. RICHARD-MOLARD. 2003. Possible role of plant phenolics in the production of trichothecences by Fusarium graminearum strains on different fractions of maize kernels. Journal of Agricultural and Food Chemistry, 51: 2826–2831. BEEKRUM, S., R. GOVINDEN, T. PADAYACHEE and B. ODHAV. 2003. Naturally occurring phenols: a detoxification strategy for fumonisin B1. Food Additives and Contaminant, 20: 490–493. BENNETT, J. W. and M. KLICH. 2003. Mycotoxins. Clinic. Microbiol. Reviews. 497–516. BROWN, R. L., A. MENKIR, Z. CHEN, D. BHATNAGAR, J. YU, H. YAO and T.E. CLEVELAND. 2013. Breeding aflatoxin-resistant maize lines using recent advances in technologies –a review. Food Additives and Contaminants, 30: 1382-1391. CARY, J. W., K. RAJASEKARAN, R. BROWN, M. LUO, Z. CHEN and D. BHATNAGAR. 2011. Developing resistance to aflatoxin in maize and cottonseed. Toxins, 3: 678-696. CHEN, Z.Y., R.L. BROWN, K.E. DAMANN and T.E. CLEVELAND. 2010. PR10 expression in maize and its effect on host resistance against Aspergillus flavus infection and aflatoxin production. Molecular Plant Pathology, 11(1): 69–81. DOSTER, M.A. and T.J. MICHAILIDES. 1995. The relationship between date of hull splitting and decay of pistachio nuts by Aspergillus species. Plant Disease, 79: 766-769. ELAHINIA, S.A. 2014. Mycology and Plant Pathology. Forth Edition. University of Guilan press. Iran. 666pp. FANI, S.R., M. MORADI, C. PROBST, H.R. ZAMANIZADEH, M. MIRABOLFATHY, M. HAIDUKOWSKI and A.F. LOGRIECO. 2014. A critical evaluation of cultural methods for the identification of atoxigenic Aspergillus flavus isolates for aflatoxin mitigation in pistachio orchards of Iran. European journal of plant pathology, 140(4): 631-642. GOLI, A.H., M. BARZEGAR and M. SAHARI. 2005. Antioxidant activity and total phenolic compounds of pistachio (Pistacia vera L.) hull extracts. Food Chemistry, 92: 521–52. GRADZIEL, T., N. MAHONEY and A. ABDALLAH. 2000. Aflatoxin production among almond genotypes is not related to either kernel oil composition or Aspergillus flavus growth rate. HortScience, 35(5): 937-939. GULCIN I., M. OKTAY, I.Ö. KUFREVIOGLU and A. ASLAN. 2002. Determination of antioxidant activity of Lichen Cetriaria islandica (L) Ach, Journal of Ethnopharmacology, 79:325-329. KLIEBENSTEIN, D.J. 2004. Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant Cell and Environment, 27: 675-684. LATHA, P., P. SUDHAKAR, Y. SREENIVASULU, P.H. NAIDU and P.V. REDDY. 2007. Relationship between total phenols and aflatoxin production of peanut genotypes under end-of-season drought conditions. Acta Physiologiae Plantarum, 29: 563-566. LIANG, X. Q., M. LUO and B.Z. GUO. 2006. Resistance mechanisms to Aspergillus flavus infection and aflatoxin contamination in peanut (Arachis hypogaea). Plant Pathology Journal, 5(1): 115-124. MAHONEY, N. and R. J. MOLYNEUX. 2004. Phytochemical inhibition of aflatoxigenicity in Aspergillus flavus by constituents of walnut (Juglans regia). Journal of Agricultural Food Chemistry, 52: 1882-1889. MAHONEY, N., R. J. MOLYNEUX and B. CAMPBELL. 2002. Reduction of aflatoxin contamination in pistachio kernels by hydrolysable tannins in the hull. Proceeding of the 2nd fungal genomic, 3rd fumonisin elimination and 15th aflatoxin elimination workshop, October 23-25, 2002, San Antonio, Texas MOGHADDAM, M. M., E. M. GOLTAPEH, H. HOKMABADI, M. HAGHDEL and A.M. MORTAZAVI. 2006. Evaluation of susceptibility of pistachio cultivars to aflatoxigenic Aspergillus flavus and aflatoxin B1 production. Acta Hortticulturae, 726:655–8. NEERGHEEN, S.V., T. BAHORUN, S.L. JEN and I.O. AROUMA. 2007. Bioefficacy of Mauritian endemic medicinal plant: Assessment of their phenolic contents and antioxidant potential. Pharmaceutical Biology, 45:9-17. NIJVELDT, R.J., E.V. NOOD, D.EC. HOORN, P.G. BOELENS and P. AM. LEEUWEN. 2001. Flavonoids: a review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74: 418-425. PIZZOLITTO, R.P., C.L. BARBERIS, J.S. DAMBOLENA, J.M. HERRERA, M.P. ZUNINO, C.E. MAGNOLI, H.R. RUBINSTEIN, J.A. ZYGADLO and A.M. DALCERO. 2015. Inhibitory effect of natural phenolic compounds on Aspergillus parasiticus growth. Hindawi publishing corporation. Journal of Chemistry. http://dx.doi.org/10.1155/2015/547925. PREMLATA, S., B. SITA, S.K. AHMAD and S. BHAGAT. 1990. Aflatoxin elaboration and nutritional deterioration in some pulse cultivars during infestation with A. flavus. Journal of food Science and Technology, 27(1): 60-62. SAMAPUNDO, S., B. De MEULENAER, D. OSEI-NIMOH, Y. LAMBONI, J. DEBEVERE and F. DEVLIEGHERE. 2007. Can phenolic compounds be used for the protection of corn from fungal invasion and mycotoxin contamination during storage? Food Microbiology, 24: 465–473. SAN, R. H. C. and R. I. M. CHAN. 1987. Inhibitory effect of phenolic compounds on aflatoxin B1 metabolism and induced mutagenesis. Mutation Research Journal, 177(2):229-239. SHEIBANI, A. 1995. Pistachio production in Iran. Acta Hoticulturae, 419:192-198 SIRANIDOU, E., Z. KANG and H. BUCHENAUER. 2002. Studies on symptom development, phenolic compounds and morphological defense responses in wheat cultivars differing in resistance to Fusarium head blight. Journal of Phytopathology, 150: 200–208. TATTINI, M., D. REMORINI, P. PINELLI, G. AGATI, E. SARASINI, M.L. TRAVERSI and R. MASSAI. 2006. Morpho-anatomical, physiological and biochemical adjustment in response rot ozone salinity stress and high solar radiation in two Mediteranean evergreen shrubs, Myrtus communis and Pistacia lentiscus. New phytologist, 170: 779-794. TOMAINO, A., M. MARTORANA, T. ARCORACI, D. MOMTELEONE, C. GIVOINAZZO and A. SAIJA. 2010. Antioxidant activity and phenolic profile of pistachio (Pistacia vera L., variety Bronte) seeds and skins. Biochimie, 92:1115-1122. WOJDYLO, A., J. OSZMIANSKI and R. CZEMERYS. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105: 940–949. WORLD HEALTH ORGANIZATION (WHO). 2002. Evaluation of certain mycotoxins in food. Fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series No. 906. Geneva. | ||
|
آمار تعداد مشاهده مقاله: 1,060 تعداد دریافت فایل اصل مقاله: 733 |
||