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اثر تنظیم‌کننده‌های رشد بر کالوس‌زایی و باززایی درون شیشه‌ای گیاه شابیزک (Atropa belladonna) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 6، دوره 27، شماره 2 - شماره پیاپی 54، اسفند 1398، صفحه 231-239 اصل مقاله (793.93 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.121460.1300 | ||
| نویسندگان | ||
| بتول زارعی1؛ زهرا تقی پور* 2؛ دانیال کهریزی3 | ||
| 1دکترای گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام | ||
| 2دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام پست الکترونیک: zahrataghipour928@gamil.com | ||
| 3دانشیار، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه | ||
| چکیده | ||
| شابیزک (Atropa belladonna) گیاهیاز خانواده سولاناسه، یکساله و دارای یکی از آلکالوئیدهای تروپانی مهم به نام هیوسیامین می‌باشد. با توجه به اهمیت دارویی و اقتصادی کشت شابیزک، پیشرفت در روش‌های کشت بافت و باززایی این گیاه در شرایطin vitroبه‌منظور آسان نمودن روش‌های انتقال ژن به این گیاه دارویی مهم می‌باشد. ازاین‌رو در این تحقیق بهینه‌سازی شرایط کشت به‌منظور تولید کالوس و باززایی گیاهAtropa belladonnaبررسی گردید. برای تولید کالوس ابتدا بذرهای استریل شابیزک در محیط کشت MS کشت شدند. پس از جوانه‌زنی بذر و رشد گیاهچه‌ها به مدت دو ماه، ریزنمونه‌های ساقه و برگ در محیط کشت MS حاوی ترکیبی از غلظت‌های هورمونی مختلف 2,4-D (0، 5/0 و 1 میلی‌گرم در لیتر) و Kin (0، 1 و 2 میلی‌گرم در لیتر) قرار گرفتند. برای بررسی درصد باززایی کالوس‌های حاصل از ریزنمونه ساقه از هورمون Kin در چهار سطح (0، 3، 5 و 8 میلی‌گرم در لیتر) در قالب طرح کاملا تصادفی با سه تکرار استفاده شد. نتایج نشان داد که از نظر تیپ ریزنمونه، ریزنمونه‌های ساقه بیشترین کالوس‌زایی را داشتند. از نظر غلظت‌های هورمونی، ترکیب دو هورمون در غلظت‌های MS+2mg/l Kin +1mg/l 2,4,D و همچنین MS+1mg/l Kin +1mg/l 2,4,D بیشترین میزان تولید کالوس را نشان دادند. افزایش غلظت هورمون Kin در محیط MS با افزایش درصد باززایی ارتباط مستقیم داشت و بیشترین میزان باززایی در غلظت 8 میلی‌گرم در لیتر با 66/36 درصد دیده شد. | ||
| کلیدواژه‌ها | ||
| باززایی؛ کالوس زایی؛ کاینتین؛ شابیزک | ||
| عنوان مقاله [English] | ||
| Effect of Growth Regulators on In Vitro Callogenesis and Regeneration of Atropa Belladonna | ||
| نویسندگان [English] | ||
| B. Zarei1؛ Z. Taghipour2؛ D. Kahrizi3 | ||
| 1Ph.D. Agronomy and Plant Breeding Department, College of Agriculture, Ilam University. Ilam. I.R. Iran | ||
| 2Ph.D. student, Agronomy and Plant Breeding Department, College of Agriculture, Ilam University. Ilam. I.R. Iran Email: Zahrataghipour928@gmail.com | ||
| 3Associ. Prof., College of Agriculture, Razi University, Kermanshah, I.R. Iran. | ||
| چکیده [English] | ||
| Belladonna (Atropa belladonna), from Solanaceae, is an annual plant and has an important tropane alkaloids called hyoscyamine. Considering the pharmacological and economic importance of A. belladonna and priority in its domestication and cultivation, advances in its tissue culture and regeneration methods of this plant under in vitro conditions is important to facilitate methods of gene transfer to this medicinal herb. So, in this research, optimization of cultivation conditions for callogenesis and regeneration of Atropa belladonna was investigated. For callogenesis, belladonna sterilized seeds were initially sown in MS medium. After seed germination and seedlings growth for two months, stem and leaf explants were cultured in MS medium including a combination of different hormonal concentrations of 2.4-D (0, 0.5 and 1 mg/l) and Kin (0, 1 and 2 mg/l).To evaluate the regeneration percentage of callus from shoot explants, Kin hormone was used in four levels (0, 3, 5 and 8 mg/l) using completely randomized design with three replications. These experiments indicated that, shoot explants had the highest callogenesis in terms of explant type. In terms of hormonal concentrations, combination of two hormones at concentrations of 2.4-D 1 mg/l + Kin 2 mg/l+MS and also, 2.4-D 1mg/l +Kin 1mg/l +MS showed the highest rate of callogenesis. Increase in the concentration of Kin hormone in MS medium was directly correlated with increase in regeneration percentage and the highest regeneration rate of 36.66% was observed at 8 mg/l. | ||
| کلیدواژه‌ها [English] | ||
| Atropa bellandona, callugenesis, kin, regeneration | ||
| مراجع | ||
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