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اثر پرایمینگ و فرسودگی بر فعالیت آنزیم‌های آنتی‌اکسیدانت و تحرک ذخایر بذر لوبیا چیتی (Phaseolus Vulgaris L.) رقم صدری | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 8، شماره 2 - شماره پیاپی 16، بهمن 1398، صفحه 19-32 اصل مقاله (881.46 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.116851.1154 | ||
| نویسندگان | ||
| طیبه سعادت1؛ محمد صدقی* 2؛ عبدالقیوم قلی پوری3؛ رئوف سیدشریفی3؛ رقیه شیخ بگلو3 | ||
| 1دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه زراعت و اصلاح | ||
| 2گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی | ||
| 3گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی | ||
| چکیده | ||
| به منظور بررسی اثر پرایمینگ و فرسودگی بر روی فعالیت آنزیم‌های آنتی‌اکسیدانت و تحرک ذخایر لوبیا، آزمایشی بصورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار اجرا گردید. تیمارها شامل فرسودگی (شاهد بدون فرسودگی، 88 و 78 درصد جوانه‌زنی) و پرایمینگ (شاهد، هیدرو پرایمینگ، پرایمینگ با جیبرلین و اسید سالیسیلیک) بود. نتایج نشان داد که فرسودگی درصد جوانه‌زنی را کاهش داد. پرایمینگ سبب کاهش تاثیر فرسودگی و بهبود درصد جوانه‌زنی گردید. با افزایش فرسودگی کارایی استفاده از ذخایر، کارایی تحرک ذخایر و کسر ذخایر مصرف شده کاهش یافت، ولی شاخص تنفس و وزن خشک باقی‌مانده بذر افزایش یافتند. میزان کاهش SRUE نسبت به تیمار شاهد فرسودگی در حدود30% بود و شاخص تنفس بذر نیز در پیش تیمار جیبرلین در حدود 24% نسبت به شاهد بدون پرایمینگ بیشتر بود. محتوای کل پروتئین بذر در پیش تیمار جیبرلین و بدون فرسودگی در حدود 32% افزایش داشت. میزان فعالیت آنزیم پراکسیداز در تیمار جیبرلین و فرسودگی 88% نسبت به شاهد در حدود 57% افزایش نشان داد. بیشترین میزان فعالیت آنزیم سوپراکسید دیسمیوتاز (2/175 واحد بر میلی گرم) در پرایمینگ با اسید سالیسیلیک و فرسودگی 88% دیده شد. حداکثر مقدار مالون دیآلدئید (46/1 میلی مول بر گرم) به تیمار بدون پرایمینگ و فرسودگی 78% ارتباط داشت. درکل، استفاده از پیش تیمار جیبرلین موجب تقویت فیزیولوژیکی بذرهای ضعیف لوبیا شد و از این تیمار می‌توان جهت افزایش بنیه بذرهای ضعیف استفاده کرد. | ||
| کلیدواژه‌ها | ||
| اسید سالیسیلیک؛ آنتی اکسیدانت؛ پرایمینگ؛ جیبرلین؛ ذخایر بذر | ||
| عنوان مقاله [English] | ||
| The effect of priming and deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri | ||
| نویسندگان [English] | ||
| Tayyebeh Saadat1؛ Mohammad Sedghi2؛ Abdolghayyoum Gholipouri3؛ Raouf Seyed Sharifi3؛ Roghayyeh Sheikhbaglou3 | ||
| 1University of Mohaghegh Ardabili, Faculty of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding | ||
| 2University of Mohaghegh Ardabili, Faculty of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding | ||
| 3Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili | ||
| چکیده [English] | ||
| To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor. | ||
| کلیدواژه‌ها [English] | ||
| Salicylic acid, antioxidant, priming, gibberellin, seed reserves | ||
| مراجع | ||
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