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Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats | ||
| Archives of Razi Institute | ||
| مقاله 13، دوره 75، شماره 1، خرداد 2020، صفحه 83-91 اصل مقاله (715.81 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.123458.1251 | ||
| نویسندگان | ||
| S. Moshkelani1؛ A. Asghari* 1؛ A. Abedi1؛ A. Jahandideh1؛ P. Mortazavi2 | ||
| 1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO4 was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO4, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (p <0.05), whereas MgSO4 decreased I/R-induced MDA (p <0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (p <0.05). Moreover, MgSO4 (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (p <0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO4 (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO4 on testicular IR injury in rats. | ||
| کلیدواژهها | ||
| Ischemia/reperfusion (I/R)؛ Magnesium sulfate؛ Rat؛ Testis | ||
| عنوان مقاله [English] | ||
| اثرات سولفات منیزیم برعملکرد بیضه متعاقب ایسکمی/ رپرفیوژن در موش صحرایی | ||
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