| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,188 |
| تعداد مقالات | 35,336 |
| تعداد مشاهده مقاله | 51,458,209 |
| تعداد دریافت فایل اصل مقاله | 91,331,908 |
Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats | ||
| Archives of Razi Institute | ||
| مقاله 13، دوره 75، شماره 1، خرداد 2020، صفحه 83-91 اصل مقاله (715.81 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.123458.1251 | ||
| نویسندگان | ||
| S. Moshkelani1؛ A. Asghari* 1؛ A. Abedi1؛ A. Jahandideh1؛ P. Mortazavi2 | ||
| 1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO4 was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO4, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (p <0.05), whereas MgSO4 decreased I/R-induced MDA (p <0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (p <0.05). Moreover, MgSO4 (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (p <0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO4 (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO4 on testicular IR injury in rats. | ||
| کلیدواژهها | ||
| Ischemia/reperfusion (I/R)؛ Magnesium sulfate؛ Rat؛ Testis | ||
| عنوان مقاله [English] | ||
| اثرات سولفات منیزیم برعملکرد بیضه متعاقب ایسکمی/ رپرفیوژن در موش صحرایی | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Agarwal, A., Durairajanayagam, D., Halabi, J., Peng, J., Vazquez-Levin, M., 2014. Proteomics, oxidative stress and male infertility. Reprod Biomed Online 29, 32-58.
Agarwal, A., Sharma, R.K., Desai, N.R., Prabakaran, S., Tavares, A., Sabanegh, E., 2009. Role of oxidative stress in pathogenesis of varicocele and infertility. Urology 73, 461-469.
Agarwal, R., Iezhitsa, I., Awaludin, N.A., Ahmad Fisol, N.F., Bakar, N.S., Agarwal, P., et al., 2013. Effects of magnesium taurate on the onset and progression of galactose-induced experimental cataract: in vivo and in vitro evaluation. Exp Eye Res 110, 35-43.
Akhondi, M.M., Mohazzab, A., Jeddi-Tehrani, M., Sadeghi, M.R., Eidi, A., Khodadadi, A., et al., 2013. Propagation of human germ stem cells in long-term culture. Iran J Reprod Med 11, 551-558.
Arena, S., Iacona, R., Antonuccio, P., Russo, T., Salvo, V., Gitto, E., et al., 2017. Medical perspective in testicular ischemia-reperfusion injury. Exp Ther Med 13, 2115-2122.
Asghari, A., Akbari, G., Beigi, A., Mortazavi, P., 2018a. Tramadol reduces testicular damage of ischemia-reperfusion rats. Anim Reprod 13, 811-819.
Asghari, A., Akbari, G., Galustanian, G., 2018b. Magnesium sulfate protects testis against unilateral varicocele in rat. Anim Reprod 14, 442-451.
Aydıner, C., Pul, M., İnan, M., Bilgi, S., Çakır, E., 2012. Can N-acetylcysteine play a role on preventing tissue damage on experimental testicular torsion. Cum Med J 34, 462-471.
Barbagallo, M., Dominguez, L.J., Galioto, A., Pineo, A., Belvedere, M., 2010. Oral magnesium supplementation improves vascular function in elderly diabetic patients. Magnes Res 23, 131-137.
Celik, E., Oguzturk, H., Sahin, N., Turtay, M.G., Oguz, F., Ciftci, O., 2016. Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats. Arch Med Sci 12, 928-934.
Chandra, A.K., Sengupta, P., Goswami, H., Sarkar, M., 2013. Effects of dietary magnesium on testicular histology, steroidogenesis, spermatogenesis and oxidative stress markers in adult rats. Indian J Exp Biol 51, 37-47.
Eghbali, M., Alavi, S.S., Asri, R.S., Khadem, A.M., 2010. Calcium, magnesium and Total Antioxidant Capacity (TAC) in seminal plasma of Water Buffalo (Bubalus bubalis) bulls and their relationships with semen characteristics. Vet Res Forum 1, 12-20.
Eshraghi, T., Eidi, A., Mortazavi, P., Asghari, A., Tavangar, S.M., 2015. Magnesium protects against bile duct ligation-induced liver injury in male Wistar rats. Magnes Res 28, 32-45.
Fakouri, A., Asghari, A., Akbari, G., Mortazavi, P., 2017. Effects of folic acid administration on testicular ischemia/reperfusion injury in rats. Acta Cir Bras 32, 755-766.
Ghalehkandi, J.G., 2018. Garlic (Allium sativum) juice protects from semen oxidative stress in male rats exposed to chromium chloride. Anim Reprod 11, 526-532.
Hadwan, M.H., Almashhedy, L.A., Alsalman, A.R., 2014. Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients. Reprod Biol Endocrinol 12, 1.
Hasturk, A.E., Harman, F., Arca, T., Sargon, M., Kilinc, K., Kaptanoglu, E., 2013. Neuroprotective effect of magnesium sulfate and dexamethasone on intrauterine ischemia in the fetal rat brain: ultrastructural evaluation. Turk Neurosurg 23, 666-671.
Hsieh, Y.Y., Chang, C.C., Lin, C.S., 2006. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int J Biol Sci 2, 23-29.
Huang, X., Qin, J., Lu, S., 2014. Magnesium isoglycyrrhizinate protects hepatic L02 cells from ischemia/reperfusion induced injury. Int J Clin Exp Pathol 7, 4755-4764.
Hwang, K., Lamb, D.J., 2012. Molecular mechanisms of antioxidants in male infertility. Male infertility, Springer, pp. 245-260.
Johnsen, S.G., 1970. Testicular biopsy score count–a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr 1, 2-25.
Kanter, M., 2010. Protective effects of melatonin on testicular torsion/detorsion-induced ischemia-reperfusion injury in rats. Exp Mol Pathol 89, 314-320.
Koksal, M., Oguz, E., Baba, F., Eren, M.A., Ciftci, H., Demir, M.E., et al., 2012. Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats. Eur Rev Med Pharmacol Sci 16, 582-588.
Korkmaz, Ş., Ekici, F., Ali Tufan, H., Aydın, B., 2013. Magnesium: Effect on ocular health as a calcium channel antagonist. J Clin Exper Invest 4, 244-251.
Masson, P., Brannigan, R.E., 2014. The varicocele. Urol Clin North Am 41, 129-144.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V., Milner, A., 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci (Lond) 84, 407-412.
Paglia, D.E., Valentine, W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70, 158-169.
Paoletti, F., Mocali, A., 1990. Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186, 209-220.
Parlaktas, B.S., Atilgan, D., Ozyurt, H., Gencten, Y., Akbas, A., Erdemir, F., et al., 2014. The biochemical effects of ischemia-reperfusion injury in the ipsilateral and contralateral testes of rats and the protective role of melatonin. Asian J Androl 16, 314-318.
Pesch, S., Bergmann, M., Bostedt, H., 2006. Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen quality. Theriogenology 66, 307-313.
Placer, Z.A., Cushman, L.L., Johnson, B.C., 1966. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analyt Biochem 16, 359-364.
Pogorelic, Z., Mustapic, K., Jukic, M., Todoric, J., Mrklic, I., Messtrovic, J., et al., 2016. Management of acute scrotum in children: a 25-year single center experience on 558 pediatric patients. Can J Urol 23, 8594-8601.
Raju, A.B., Challa, S.R., Akula, A., Kiran, K., Harinadh, G.B., 2011. Evaluation of oxidant and anti-oxidant balance in experimentally induced testicular injury by ischemia reperfusion in rats. Eur J Gen Med 8, 117-121.
Ravn, H.B., Moeldrup, U., Brookes, C.I., Ilkjaer, L.B., White, P., Chew, M., et al., 1999. Intravenous magnesium reduces infarct size after ischemia/reperfusion injury combined with a thrombogenic lesion in the left anterior descending artery. Arterioscler Thromb Vasc Biol 19, 569-574.
Romani, A., 2007. Regulation of magnesium homeostasis and transport in mammalian cells. Arch Biochem Biophys 458, 90-102.
Sahin, Z., Bayram, Z., Celik-Ozenci, C., Akkoyunlu, G., Seval, Y., Erdogru, T., et al., 2005a. Effect of experimental varicocele on the expressions of Notch 1, 2, and 3 in rat testes: an immunohistochemical study. Fertil Steril 83, 86-94.
Sahin, Z., Bayram, Z., Celik-Ozenci, C., Akkoyunlu, G., Seval, Y., Erdogru, T., et al., 2005b. Effect of experimental varicocele on the expressions of Notch 1, 2, and 3 in rat testes: an immunohistochemical study. Fertil Steril 83, 86-94.
Valsa, J., Skandhan, K.P., Khan, P.S., Sumangala, B., Gondalia, M., 2012. Split ejaculation study: semen parameters and calcium and magnesium in seminal plasma. Cent European J Urol 65, 216-218.
Wasowicz, W., Neve, J., Peretz, A., 1993. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 39, 2522-2526.
Wolf, F.I., Trapani, V., Simonacci, M., Boninsegna, A., Mazur, A., Maier, J.A., 2009. Magnesium deficiency affects mammary epithelial cell proliferation: involvement of oxidative stress. Nutr Cancer 61, 131-136.
Wong, W.Y., Flik, G., Groenen, P.M., Swinkels, D.W., Thomas, C.M., Copius-Peereboom, J.H., et al., 2001. The impact of calcium, magnesium, zinc, and copper in blood and seminal plasma on semen parameters in men. Reprod Toxicol 15, 131-136.
Yavuz, Y., Mollaoglu, H., Yurumez, Y., Ucok, K., Duran, L., Tunay, K., et al., 2013. Therapeutic effect of magnesium sulphate on carbon monoxide toxicity-mediated brain lipid peroxidation. Eur Rev Med Pharmacol Sci 17, 28-33.
Yulug, E., Turedi, S., Alver, A., Turedi, S., Kahraman, C., 2013. Effects of resveratrol on methotrexate-induced testicular damage in rats. Sci World J 2013, 489659. | ||
|
آمار تعداد مشاهده مقاله: 2,393 تعداد دریافت فایل اصل مقاله: 3,235 |
||
