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Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran | ||
| Archives of Razi Institute | ||
| مقاله 9، دوره 75، شماره 1، خرداد 2020، صفحه 47-54 اصل مقاله (699.94 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.122733.1227 | ||
| نویسندگان | ||
| S. Zarrabi Ahrabi1؛ R. Madani* 1، 2؛ B. Shemshadi1؛ Sh. Ranjbar Bahadori3؛ H. Hashemzadeh Farhang4 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Proteomics & Biochemistry section Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 3Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran | ||
| 4Department of Parasitology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran | ||
| چکیده | ||
| Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran. The genetic diversity of this parasite is very high, particularly in areas where the disease is endemic. It has been suggested in the literature from different parts of the world that diverse factors, such as parasite life cycle, transmission pathways, pathologic disease, immunization, and disease control can be affected by the genetic diversity of the parasite. Various studies indicated sheep strain G1 as the most common genotype throughout the world. This strain is commonly found in the liver and lung repeatedly causing echinococcosis in humans, sheep, and cattle. The present study was conducted to determine the genetic affinity between the protoscolex of E. granulosus in humans and sheep in East Azerbaijan province, Iran for the first time. A total of 120 hydatid cyst samples were collected, 60 of which were from people who referred to the hospitals of East Azerbaijan and 60 were from the sheep slaughtered in Tabriz slaughterhouse. Following DNA extraction, certain regions of the cox1 gene were amplified and evaluated by the polymerase chain reaction. The replicated parts in all isolates had the same size of 450 bp. Electrophoresis was followed by selecting a total of 60 suitable samples, including 30 human samples and 30 sheep samples and sending them for genome sequencing. The overlap of the samples was investigated using the BLAST software. The results of BLAST, sequencing, and overlap demonstrated a genetic linkage of approximately 91.76% between the protoscolex of E. granulosus in human and sheep. | ||
| کلیدواژهها | ||
| DNA extraction؛ Genetic affinity؛ Hydatid cyst؛ PCR؛ Sequencing | ||
| عنوان مقاله [English] | ||
| تعیین قرابت ژنتیکی پروتواسکولکس اکینوکوکوس گرانولوزوس در انسان و گوسفند دراستان آذربایجان شرقی | ||
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| مراجع | ||
|
Acha, P.N., Szyfres, B., 2003. Zoonoses and communicable diseases common to man and animals, Pan American Health Org, New York, pp. 184-199.
Alvarez Rojas, C.A., Romig, T., Lightowlers, M.W., 2014. Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasitol 44, 9-18.
Bayat, A., Shirazi, S., Zarabi, S., Nejhad-Partovi, A., 2014. The epidemiologic survey of operated patients with haydatid cyst in Emam Reza medical and research and training hospital in Tabriz city during 2011-2012. 6th International Congress of Laboratory and Clinic, Tehran, Iran, pp. 287-294.
Boufana, B., Lett, W.S., Lahmar, S., Buishi, I., Bodell, A.J., Varcasia, A., et al., 2015. Echinococcus equinus and Echinococcus granulosus sensu stricto from the United Kingdom: genetic diversity and haplotypic variation. Int J Parasitol 45, 161-166.
Bowles, J., Blair, D., McManus, D.P., 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54, 165-173.
Casulli, A., Interisano, M., Sreter, T., Chitimia, L., Kirkova, Z., La Rosa, G., et al., 2012. Genetic variability of Echinococcus granulosus sensu stricto in Europe inferred by mitochondrial DNA sequences. Infect Genet Evol 12, 377-383.
Fasihi Harandi, M., Budke, C.M., Rostami, S., 2012. The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis 6, e1915.
John, D.T., Petri, W.A., 2006. Markell and Voge's medical parasitology-e-book, Elsevier Health Sciences, Philadelphia, USA, pp. 287-296.
McManus, D., 2002. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Trans R Soc Trop Med Hyg 96, S151-S157.
Mitrea, I.L., Ionita, M., Costin, II, Predoi, G., Avram, E., Rinaldi, L., et al., 2014. Occurrence and genetic characterization of Echinococcus granulosus in naturally infected adult sheep and cattle in Romania. Vet Parasitol 206, 159-166.
Neva, F.A., Brown, H.W., 1994. Basic clinical parasitology, Appleton & Lange, New York, USA, pp, 217-224.
Nikmanesh, B., Mirhendi, H., Ghalavand, Z., Alebouyeh, M., Sharbatkhori, M., Kia, E., et al., 2014. Genotyping of Echinococcus granulosus isolates from human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran. Iran J Parasitol 9, 20-27.
Oudni, M., M'Rad, S., Mekki, M., Belguith, M., Cabaret, J., Pratlong, F., et al., 2004. Genetic relationships between sheep, cattle and human Echinococcus infection in Tunisia. Vet Parasitol 121, 95-103.
Parsa, F., Fasihi Harandi, M., Rostami, S., Sharbatkhori, M., 2012. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol 132, 308-312.
Pezeshki, A., Akhlaghi, L., Sharbatkhori, M., Razmjou, E., Oormazdi, H., Mohebali, M., et al., 2013. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol 87, 387-391.
Pissuwan, D., Valenzuela, S.M., Miller, C.M., Cortie, M.B., 2007. A golden bullet? Selective targeting of Toxoplasma gondii tachyzoites using antibody-functionalized gold nanorods. Nano Lett 7, 3808-3812.
Shahnazi, M., Hejazi, H., Salehi, M., Andalib, A.R., 2011. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop 117, 47-50.
Shamsi, M., Dalimi, A., Khosravi, A., Ghafarifar, F., 2016. The phylogenetic similarity of hydatid cyst isolated from humans and sheep in Ilam Province southwest of Iran. Comp Clin Pathol 25, 1221-1226.
Spotin, A., Mahami-Oskouei, M., Harandi, M.F., Baratchian, M., Bordbar, A., Ahmadpour, E., et al., 2017. Genetic variability of Echinococcus granulosus complex in various geographical populations of Iran inferred by mitochondrial DNA sequences. Acta Trop 165, 10-16. | ||
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