Razinataj, M., Khodakaramian, G. (2016). Relief and Pathogenicity of Xanthomonas citri subsp. malvacearum from infected cotton leaves after 9 years and investigation of biochemical characteristics of isolates. , 3(2), 65-72. doi: 10.22092/ijcr.2016.106776
Mohamad Razinataj; Gholam Khodakaramian. "Relief and Pathogenicity of Xanthomonas citri subsp. malvacearum from infected cotton leaves after 9 years and investigation of biochemical characteristics of isolates". , 3, 2, 2016, 65-72. doi: 10.22092/ijcr.2016.106776
Razinataj, M., Khodakaramian, G. (2016). 'Relief and Pathogenicity of Xanthomonas citri subsp. malvacearum from infected cotton leaves after 9 years and investigation of biochemical characteristics of isolates', , 3(2), pp. 65-72. doi: 10.22092/ijcr.2016.106776
Razinataj, M., Khodakaramian, G. Relief and Pathogenicity of Xanthomonas citri subsp. malvacearum from infected cotton leaves after 9 years and investigation of biochemical characteristics of isolates. , 2016; 3(2): 65-72. doi: 10.22092/ijcr.2016.106776

Relief and Pathogenicity of Xanthomonas citri subsp. malvacearum from infected cotton leaves after 9 years and investigation of biochemical characteristics of isolates

پژوهش های پنبه ایران
Article 5, Volume 3, Issue 2, March 2016, Pages 65-72    PDF (1.1 M)
Document Type: Research Paper
DOI: 10.22092/ijcr.2016.106776
Authors
Mohamad Razinataj* 1; Gholam Khodakaramian2
1Cotton Research Institute of Iran
2College of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Abstract
Cotton bacterial blight is a important and quarantine diseases of cotton in Iran that appeared in cotton fields of Golestan province in 2005. Samples of infected leaves collected and served in room temperature. In 2014, sections from infected leaves washed with tap water, then were placed in a few drop sterile distilled water in room temperature for five hours. The suspension streaked on NA medium. After 48-72 hours isolated bacteria colonies yellow, circle and 1 mm diameter. The bacteria were negative in gram, oxidase and fermentative growth tests. All of isolates were positive oxidative growth and starch hydrolysis. The isolates used from glucose, inulin, urate, malonate, lactate and tripton but did not used from trehalose, L-rhamnose, D-raffinose, fucose, maltose, L.tartrate, D-galacturonate, acetate, nicotinate, glycine, casein, dulcitol, L-threonine, and adonitol. Isolates were variable in utilization of arabinose, D-fructose, D-xylose, melezitose, melibiose, guanine, L-cysteine, L-tryptophan, L-histidine, betaine, β-alanine, L-valine, D-sorbitol, citrate and L-maleate. In Pathogenicity test, bacterial suspension injected with sterile syringe into epidermis leaf of Golestan cotton cultivar. The water-soaked spots observed after 3 days that extended and revealed vein blight. The importance oftherole ofinfectedplant debrisin theincidence andspread of the diseaseisofparticular importance.
Keywords
Cotton; Bacterial blight; Survival and Xanthomonas citri subsp. malvacearum
References
  1. Bradbury, J.F. 1986. Guide to Plant Pathpgenic Bateria. C.A.B. International Mycologocal Institue, Ferrylane, Kew, Surrey, England. 332 pp.
  2. Brinkerhoff, L. A. 1970. Variation in Xanthomonas malvacearum and its relation to control. Ann. Rev. Phytopathol. 8:85-110.
  3. Brinkerhoff, L. A. and Hunter, R. E. 1963. Internally infected seed as a source of inoculum for the primary cucle of bacterial blight of cotton. Phytopathlogy, 53: 1397-1401.
  4. Fahy, P.C. and Persley, G.J. 1983. Plant Bacterial Disease: A Diagnostic Guide. Academic Press. Sydney. 393 pp.
  5. Hilocks, R.J. 1992. Cotton Diseases. C.A.B. International. 415pp.
  6. Hirano, S.S. and Upper, C.D. 1983. Ecology and Epidemiology of foliar bacterial plant pathogens. Annual Review of phytopathology, 21.243-69.
  7. Holts, J.H., Krieg, N.R. Sneath, P. H.A., Stley, J.T. and Williams, S.T. 1994. Bergeys Manual of Determinative Bacteriology. 9th Edition. The Williams & Wikins Co., Baltimore, MD. USA. 1: 787 pp.
  8. Islam, Z., Khalequzzaman, K.M., Rahman, G.M.M., Tahasinul, M.I. and hossain, M. 2003. Effect of chemicals in controlling bacterial blight of cotton. Asian J. Plant Sci. 2:569-543.
  9. Jahid, I.K. and Ha, S.D. A review of microbial biofilms of produce: Future challenge to food safety. Food Science and Biotech. 21:299-316.

10. King, E.D., Ward, M.K. and Raney, D.E. 1954. Two simple media for demonstration of pyocyanin and fluorescin. J. Lab. Clinic. Medic. 44:301-307.

11. Klement, Z., Farkas, G.L. and Loverkovich, L. 1964. Hypersensitive reaction induced by phytopathogeic bacteria in the tobacco leaf. Phytopathology 54:474-477.

12. Lelliot, R.A., Billing, E. and Hayward, A.C. 1966. A determinative scheme for the fluorescent plant pathogenic pseudomonas. J. Appl. Bacteriol. 29:470-489.

13. Mehta, Y.R. 1990. Management of Xanthomonas campestrispv. undulosa through cereal seed testing. Seed Science and Technol. 18: 467-476.

14. O,Too;e, G. Kaplan, H.B. and Kolter, R. 2000. Biofilm formation as microbial development. Annu. Rev. Microbiol. 54:49-79.

15. Schaad, N.W., Jones, J.B. and Chun, W. (eds). 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3nd edition. Aps Press. St. Minnesots, USA. 373pp.

16. Srinivasan, K.V. 1994. Cotton Diseases. CIRCOT Press. 314pp.

17. Suslow, T.V., Schroth, M.N. and Isaka, M. 1982. Application of rapid method for Gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology, 72: 917-918.

Statistics
Article View: 250
PDF Download: 198


counter
Journal management system. designed by sinaweb