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بررسی اثر باسیلوس آمیلولیکوفاسیانس بر کاهش اثرات مضر آفلاتوکسین B1 در جیره جوجه های گوشتی | ||
| علوم دامی | ||
| مقاله 16، دوره 32، شماره 124، آذر 1398، صفحه 213-228 اصل مقاله (1.72 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2018.123443.1776 | ||
| نویسندگان | ||
| منصوره عبدالملکی1؛ علی اصغر ساکی* 2؛ محمد یوسف علیخانی3 | ||
| 1دانشجو دکتری تغذیه طیور دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| 2استاد گروه علوم دامی دانشکده کشاورزی دانشگاه بوعلی سینا همدان. | ||
| 3استاد گروه میکروبشناسی دانشکده پزشکی، دانشگاه علومپزشکی همدان | ||
| چکیده | ||
| در این مطالعه بهمنظور بررسی اثر پروبیوتیک باسیلوس آمیلولیکوفاسیانس در کاهش اثرات مضر آفلاتوکسین B1 از 480 قطعه جوجه گوشتی یکروزه (نر و ماده) سویه راس 308 در قالب طرح کاملا تصادفی بهصورت فاکتوریل (2×2×2) با 8 تیمار، 4 تکرار و تعداد 15 قطعه در هر تکرار استفاده شد. فاکتورها شامل جنسیت (خروس و مرغ)؛ 2) آفلاتوکسین B1 در دو سطح (صفر و 500 میکروگرم در کیلوگرم)؛ 3) پروبیوتیک باسیلوس آمیلولیکوفاسیانس در دو سطح (صفر و 109 واحد تشکیل دهنده کلنی در میلیلیتر) بودند. عملکرد، فعالیت آنزیمهای کبدی، ایمنی و برخی شاخصهای خونی تحت تاثیر جنسیت قرار نگرفتند (P>0.05). جوجههایی که جیره حاوی آفلاتوکسین B1 خورده بودند، کاهش وزن و مصرف خوراک معنیداری نسبت به سایر گروهها داشتند (05/0 p <). ضریب تبدیل غذایی در کل دوره تحت تاثیر تیمارهای آزمایشی قرار نگرفت (05/0P>). فعالیت آنزیمهای آسپارتات آمینوترانسفراز، آلانین آمینوترانسفراز،آلکالین فسفاتاز و لاکتات دهیدروژناز افزایش معنیداری در تیمار آفلاتوکسین B1 نشان دادند (05/0 p <). تعداد گلبولهای سفید و قرمز و درصد هماتوکریت و هموگلوبین در گروه آفلاتوکسین B1 نسبت به سایر تیمارها بهطور معنیداری کاهش یافتند (05/0 p <). کمترین عیار پادتن علیه بیماری نیوکاسل و گلبول قرمز گوسفند و همچنین کمترین ضخامت پوست در پاسخ به فیتوهماگلوتنین در گروه آفلاتوکسین B1 مشاهده شد(05/0 p <). با توجه به نتایج، مکمل کردن باسیلوس آمیلولیکوفاسیانس به جیره آلوده به آفلاتوکسین B1 توانست عملکرد را بهبود بخشد و فعالیت آنزیمهای کبدی و پاسخ ایمنی را در مقایسه با جیره آلوده به آفلاتوکسین B1 افزایش دهد (05/0 p <). | ||
| کلیدواژهها | ||
| جوجههای گوشتی؛ آفلاتوکسین B1؛ باسیلوسآمیلولیکوفاسیانس؛ عملکرد؛ پارامترهای خونی وپاسخ ایمنی | ||
| عنوان مقاله [English] | ||
| Effects of Bacillus amyloliquefaciens on reducing adverse effects of aflatoxin B1 in broilers | ||
| نویسندگان [English] | ||
| Mansoureh Abdolmaleki1؛ Ali asghar Saki2؛ Mohammad yousef Alikhani3 | ||
| 1Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina university | ||
| 2Department of Animal Science, Bu-Ali Sina university, Faculty of Agriculture | ||
| 3Department of microbiology, medical school, university of medical sciences, Hamedan, Iran. | ||
| چکیده [English] | ||
| A study was conducted to evaluate the effects of Bacillus amyloliquefaciens on adverse effects of aflatoxin B1 in broiler chickens. A total of 480 1-d-old broiler Ross 308 (male and female) were completely randomized disgine with a 2× 2 × 2 factorial arrangements (sex and feed additives) with 4 replicates of 15 birds. Factors includes sex (male and female); probiotic (control, 108 cfu/mL B. amyloliquefaciens); aflatoxin (control, 500 AFB1 µg/kg), Performance, the activity of liver enzymes, immune and some blood parameters were not affected by sex (p> 0.05). Body weight and feed intake of aflatoxin B1 group were significantly decreased compared other (p < 0.05). There was no significantly drffenece in feed conversion ratio compared with other groups during total period (p>0.05). Activity of liver enzymes including aspartate amino transferase, alanine amino transferase, alkaline phosphatase and lactate dehydrogenase were significantly increased in aflatoxin B1 group (p < 0.05). The counts of red blood cells and withe blood cells, hematocrite and hemoglobin percentages were significantly decreased in aflatoxin B1 group compared with other (p < 0.05). The aflatoxin B1 group has shown the lowest of antibody production against newcastle disease and sheep blood red cell also, the lowest of skin thickness in responding to phytohaemagglutinin (p < 0.05). In conclusion, the supplementation of B. amyloliquefaciens to contaminated food could be improved performance and increased the activity of liver enzyme and immune response in broilers. | ||
| کلیدواژهها [English] | ||
| broiler chickens, aflatoxin B1, B. amyloliquefaciens, performance, blood parameters and immune | ||
| مراجع | ||
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