| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,188 |
| تعداد مقالات | 35,336 |
| تعداد مشاهده مقاله | 51,459,641 |
| تعداد دریافت فایل اصل مقاله | 91,332,256 |
شناسایی مولکولی ویروس پیسک توتفرنگی (Strawberry mottle virus) از مزارع توتفرنگی استان کردستان بر اساس منطقه 3΄NCR | ||
| آفات و بیماری های گیاهی | ||
| مقاله 9، دوره 87، شماره 1 - شماره پیاپی 108، شهریور 1398، صفحه 107-116 اصل مقاله (697.3 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.125077.1266 | ||
| نویسندگان | ||
| نسرین قادری زندان1؛ محمد حاجی زاده* 2 | ||
| 1گروه گیاهپزشکی دانشگاه کردستان، سنندج، ایران | ||
| 2هیات علمی دانشگاه کردستان | ||
| چکیده | ||
| در تابستان 1397، علائم ویروسی شامل پیسک، بدشکلی و سوختگی حاشیه برگها از مزارع توتفرنگی استان کردستان مشاهده و تعداد 48 نمونه برگی جمعآوری شد. ابتدا برخی نمونهها به گیاه محک Fragaria vesca با استفاده از شته توتفرنگی (Chaetosiphon fragaefolii) تلقیح و علایم مطابق با Strawberry mottle virus (SMoV) ظاهر شد. سپس، آر ان ا کل از نمونهها با روش سیلیکا استخراج و دی ان ا مکمل آنها با آغازگرهای تصادفی ششتایی ساخته شد. PCR با آغازگرهای اختصاصی SMoV- Sm2ncr1a و SMoV-Smdetncr4a انجام و قطعهای به طول 461 جفت باز در 28 نمونه از 48 نمونه فوق تکثیر گردید. بهمنظور اطمینان از نتیجه RT-PCR و بررسی مشخصات مولکولی، پنج جدایه براساس منطقه جغرافیایی، رقم میزبان و نوع علایم انتخاب و پس از الحاق به پلاسمید pTG-19 در باکتری Escherichia coliانتقال و پلاسمیدهای نوترکیب همسانه شده تعیین توالی شدند. میانگین فاصله ژنتیکی در بین جدایههای در این تحقیق در سطح نوکلئوتیدی 002/0 ± 005/0 و با سایر جدایههای موجود در بانک ژن 008/0 ± 084/0 بود. مقایسه دوبه دوی توالیهای نوکلئوتیدی نشان داد که بیشترین و کمترین فاصله ژنتیکی بهترتیب میان جدایه K3 با جدایه NSper51 از کانادا (015/0 ± 093/0) و جدایه SQA یا AG با جدایه NB926 از کانادا (007/0 ± 019/0) وجود داشت.این اولین گزارش از شناسایی این ویروس در مزارع توتفرنگی استان کردستان میباشد. | ||
| کلیدواژهها | ||
| آلودگی ویروسی؛ تبارزایی؛ توت فرنگی؛ فاصله ژنتیکی | ||
| عنوان مقاله [English] | ||
| Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄NCR | ||
| نویسندگان [English] | ||
| Nasrin Ghaderi Zandan1؛ Mohammad Hajizadeh2 | ||
| 1Plant Protection Department, University of Kurdistan, Sanandaj, Iran | ||
| چکیده [English] | ||
| In the summer of 2018, viral symptoms corresponded to Strawberry mottle virus (SMoV) in some strawberry fields were observed and 48 leaf samples/plants were collected from different fields from Kurdistan Province. In the preliminary test, some samples were inoculated to Fragaria vesca by strawberry aphid (Chaetosiphon fragaefolii) and SMoV-symptoms were observed after about two weeks. Then, total RNA of samples was extracted by silica-capture method and subjected to cDNA synthesize with random hexamer primers. RT-PCR was done by specific SMoV pair primer and amplified fragments were observed on 1.2% agarose gels. RT-PCR results were showed that 28 out of 48 samples were infected by SMoV. Based on the geographical origin, cultivar and symptoms, five isolates were selected, ligated to pTG-19 and transformed to Escherichia coli. Recombinant plasmids were sequenced and analyzed for molecular characterization and phylogenetic studies on SMoV 3΄NCR. Mean genetic distance between the isolates from Iran was 0.005 ± 0.002 but between these isolates and the other isolates available in GenBank was 0.084 ± 0.008. The highest and lowest genetic distance was found between K3 with NSper51 (0.093 ± 0.008) and SQA/AG with NB926 (0.019 ± 0.017) from Canada, respectively. This is the first report on occurrence of SMoV on strawberry in Kurdistan Province. | ||
| کلیدواژهها [English] | ||
| Genetic distance, phylogeny, strawberry, viral infectio | ||
| مراجع | ||
|
CHANG L., Z. ZHANG, H. YANG, H. LI and H. DAI, 2007. Detection of strawberry RNA and DNA viruses by RT-PCR using total nucleic acid as a template. Journal of Phytopathology, 155: 431-436. CONVERSE, R. H. 1981. Infection of cultivated strawberries by Tomato ringspot virus. Phytopathology, 71:1149-1152. DAVIS, T. M., B. DENOYES-ROTHAN and E. LERCETEAU-KOHLER, 2007. Strawberry. In: Genome mapping and molecular breeding in plants, Kole, C. (ed), Vol IV, Fruits and nuts. FAO, 2016. FAOSTAT Database results from FAO website. Food and Agriculture Organization of the United Nations. FATAHI, F, 2005. Molecular identification of strawberry viruses from Iran and their elimination. MSc. thesis in plant pathology. Faculty of Agriculture, Tarbiat Modarres University. (in Persian with English summary). FOISSAC, X., L. SAVALLE-DUMAS, P. GENTIT, M. J. DULUCQ and T. CANDRESSE, 2000. Polyvalent detection of fruit tree Tricho, Capillo and Faveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Acta Horticulture, 357: 52-59. FRAZIER, N. W. and E. S. SYLVESTER, 1960. Half-lives of transmissibility of two aphid-borne viruses. Virology, 12: 233–244. FROGER, A. and J. Hall, 2007. Transformation of plasmid DNA into E. coli using the heat shock method. Journal of Visualized Experiments, 6:253. GAO, R., Y. Xu, T. CANDRESSE, Z. HE, S. LI, Y. MA and M. LU, 2017. Further insight into genetic variation and haplotype diversity of Cherry virus A from China. PLOS ONE, 12(10):e0186273 HAJIZADEH, M. 2018. Molecular detection and phylogenetic analyses of Strawberry crinkle virus in strawberry fields of Kurdistan province based on the partial polymerase- gene sequences. Applied Research in Plant Pathology, 7(2): 95- 105. (in Persian with English summary) HALL, T. A. 1999. “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT”. Nucleic Acids Symposium Series, 41: 95-98. KRCZAL, H. 1988. Strawberry crinkle virus. Pp. 78-79, In: Smith IM, Dunez J, Lelliott RA, Phillips DH, Archer SA (eds.) European handbook of plant diseases Blackwell Scientific Publications. Oxford, UK. MARTIN, R. R. and I. E. TZANETAKIS, 2006. Characterization and recent advances in detection of strawberry viruses. Plant Disease, 90: 384–396. MARTIN, R.R. and S. SPIEGEL, 1998. “Strawberry Mottle Virus.” In Compendium of Strawberry Diseases, edited by J. L. Maas, 66-67. 2nd ed. St. Paul: APS Press. MELLOR, F. C., H. KRCZAL, N. W. FRAZIER, T. J. MORRIS, E. S. SYLVESTER, J. RICHARDSON, R. H. CONVERSE, R. R. MARTIN, S. SPIEGEL and J. R. MCGREW, 1987. “Aphid-Borne Diseases.” In Virus Diseases of Small Fruits, edited by R. H. Converse, 10-31. USDA Agricultural Handbook No. 631. U.S. Department of Agriculture-Agricultural Research Service. Washington, D.C. USDA. MICHAEL, G. and J. SAMBROOK, 2012. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 4th edition, 1885 P. NASIRINIA, G. H., R. POURRAHIM, S. A. ELAHINIA, A. ROUHIBAKHSH and SH. FARZADFAR, 2016. Incidence of Strawberry important virus diseases in Guilan and Mazandaran Provinces. Applied Entomology and Phytopathology, 84(1): 31-42. POREBSKI, S., L. G. BAILEY and B. R. BAUM, 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter, 15: 8-15. QIN, Y., J. A. TEIXEIRA DA SILVA, L. ZHANG and S. ZHANG, 2007. Transgenic strawberry: State of the art for improved traits. Biotechnology Advances, 26: 219–232. RICHARDSON J., N. W. FRAZIER and E. S. SYLVESTER, 1972. Rhabdovirus particles associated with Srawberry crinkle virus. Phytopathology, 62: 491–492. ROZAS, J. 2009. DNA Sequence Polymorphism Analysis using DnaSP. In Bioinformatics for DNA Sequence Analysis; Methods in Molecular Biology Series Vol. 537, ed. D. Posada, 337-350. USA: NJ, Humana Press. SIPAHIOGLU, H. M., M. OCAK and M. USTA, 2007. Comparison of Three Conventional Extraction Methods for the Detection of Plant Virus/Viroid RNAs from Heat Dried High-phenolic Host Leaves. Asian Journal of Plant Sciences, 6: 102-107. THOMPSON J. R., S. WETZEL, M. M. KLERKS, D. VASˇKOVA, C. D. SCHOEN, J. S. PAK and W. JELKMANN, 2003. Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. Journal of Virological Methods,111(2): 85-93. THOMPSON, J. R. and W. JELKMANN, 2003. The detection and variation of strawberry mottle virus. Plant Disease, 87: 385-390. THOMPSON, J. R., 1 G. LEONE, J. L. LINDNER, W. JELKMANN and C. D. SCHOEN, 2002. Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses. Journal of General Virology, 83: 229- 239. THOMPSON, J. R., I. DASGUPTA, M. FUCHS, T. IWANAMI, A. V. KARASEV, K. PETRZIK, H. SANFAÇON, I. TZANETAKIS, R. VAN DER VLUGT, T. WETZEL and N. YOSHIKAWA, 2017. ICTV Report Consortium. ICTV Virus Taxonomy Profile: Secoviridae. Journal of General Virology, 98(4):529-531. TZANETAKIS, I. E. and R. MARTIN, 2006. Characterization and recent advances in detection of strawberry viruses. Plant Disease, 90(4):384-396. TZANETAKIS, I. E. and R. MARTIN, 2013. Expanding field of strawberry viruses which are important in North America. International Journal of Fruit Science, 13: 184-195. WARD, L. I., B. D. QUINN, J. TANG, T. WEI and G. R. G. CLOVER. 2008. Strawberry mottle virus detected on strawberry in New Zealand. 17: 3. ZACHARZEWSKA, B., A. PRZEWODOWSKA and K. TREDER, 2014. The adaptation of silica capture RT-PCR for the detection of Potato virus Y. American Journal of Potato Research. 91:525-531. | ||
|
آمار تعداد مشاهده مقاله: 912 تعداد دریافت فایل اصل مقاله: 606 |
||