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مطالعه الگوی بیان MicroRNAهای دخیل در تحمل تنش خشکی در اجداد و خویشاوندان وحشی و اهلی گندم | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 2، دوره 27، شماره 1 - شماره پیاپی 53، شهریور 1398، صفحه 1-14 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.120248 | ||
| نویسندگان | ||
| بتول شهیدی1؛ جعفر احمدی2؛ صدیقه فابریکی اورنگ* 3 | ||
| 1دانشجوی کارشناسی ارشد ژنتیک و بهنژادی گیاهی، دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران | ||
| 2استاد، هیئت علمی گروه ژنتیک و بهنژادی گیاهی، دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران | ||
| 3استادیار، هیات علمی دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.1.53.1.1605.1606 miRNA ها، مولکولهای کوچک غیرکدکننده هستند که بیان ژنها را از طریق هضم mRNAیا ممانعت از ترجمه تنظیم میکنند. این مولکولها با تنظیم دقیق بیان ژنها، امکان پاسخ مناسب گیاه را به تغییرات مراحل رشدی یا شرایط محیطی فراهم میکنند. در این تحقیق با استفاده از تکنیک Real Time-PCR الگوی بیان miRNA159، miRNA160 و miRNA398 به همراه ژن هدف miRNA398 (CSD) در شش گونه متعلق به جنسهای تریتیکوم و آژیلوپس در کنار دو رقم گندم نان شاهد حساس (دریا) و متحمل به خشکی (سیروان)، در شرایط عدم تنش (FC=100%) و تنش خشکی (FC=25%) مورد بررسی قرار گرفتند. تجزیه qRT-PCR نشان داد که تحت تنش خشکی هر سه miRNA افزایش بیان معنی داری (P≤0.01) نسبت به شرایط عدم تنش داشتند. در بین گونههای مورد بررسی بیشترین میزان بیان miRNA159 در گونه Ae.tauschii، miRNA160 در گونه T.urartu، miRNA398 در گونه T.durum و ژن CSD در گونه T.durum مشاهده شد. در مقایسه میانگین اثر متقابل گونه و تنش بیشترین درصد افزایش بیان برای هر سه miRNA در شرایط تنش خشکی در سه گونه T.durum، T.urartu و Ae.tauschii مشاهده شد. همچنین بیشترین میزان افزایش بیان CSD در گونههای T.urartu، T.durum و Ae.tauschii بهترتیب با 62/6، 21/6 و 038/6 برابر نسبت به شرایط عدم تنش مشاهده گردید. در جمعبندی مشخص شد که سه گونه T.urartu، T.durum و Ae.tauschii به دلیل برتری از لحاظ miRNAهای عامل ایجاد تحمل میتوانند کاندیدهای مناسبی برای غنی سازی ژرمپلاسم گندم نان و اصلاح برای تحمل تنش خشکی باشند. | ||
| کلیدواژهها | ||
| گندم وحشی؛ تنش خشکی؛ miRNA؛ PCR در زمان واقعی | ||
| عنوان مقاله [English] | ||
| The Study of microRNAs expression pattern involved in drought stress tolerance in ancestors and wild-domestic relatives of wheat | ||
| نویسندگان [English] | ||
| B. Shahidi1؛ J. Ahmadi2؛ Sedigheh Fabriki-Ourang3 | ||
| 1M.Sc. Student of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
| 2Professor, Department of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran | ||
| 3Assistant Professor, Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1398.27.1.53.1.1605.1606 MicroRNAs are small non-coding molecules that regulate the expression of genes through mRNA digestion and/or translation inhibition. By accurately regulation the expression of the genes, these molecules allow the plant to respond appropriately to the changes in growth stages or environmental conditions. In this study, the miRNA159, miRNA160, miRNA398, and the miRNA398 targeted gene (CSD) were investigated in six species belonging to the Triticum and Aegilops species, along with the susceptible (DARYA) and tolerant (SIRVAN) check cultivars under drought (FC=25%) and non-stress (FC = 100%) conditions using real-time PCR. The qRT-PCR analysis showed that all three miRNAs had a significant (P≤0.01) increase in expression under drought stress. The highest expression of miRNA159, miRNA160, miRNA398, and CSD gene was observed in the species Ae.tauschii, T.urartu, T.durum, and T.durum, respectively. By the mean comparing of species × stress interaction, the highest expressions were observed in the three species T.durum, T.urartu and Ae.tauschii for all three miRNAs under drought stress. Also, the highest increase in CSD expression under drought stress was observed in T.urartu, T.durum and Ae.tauschii equal to 6.62, 6. 21 and 0.63 fold in compared to the non-stress condition, respectively. It was concluded that the species T.urartu, T.durum and Ae.tauschii due to their superiority in terms of containing miRNAs causer tolerance, could be suitable candidates for bread wheat germplasm enrichment and breeding to drought stress tolerance. | ||
| کلیدواژهها [English] | ||
| Drought Stress, MiRNA, Real-time PCR, Wild Wheat | ||
| مراجع | ||
|
- Akdogan, G., Tufekci, E.D., Uranbey, S. and Unver, T., 2015. miRNA-based drought regulation in wheat. Functional & integrative Genomics,16: 221-233. - Aydın, S., Buyuk, I. and Aras, E.S., 2014. Expression of SOD gene and evaluating its role in stress tolerance in NaCl and PEG stressed Lycopersicum esculentum. Turkish Journal of Botany, 38: 89–98. - Barrera-Figueroa, B.E. and Gao, L., 2012. High throughput sequencing reveals novel and abiotic stress regulated microRNAs in the inflorescences of rice. BMC Plant Biology, 12: 132-137. - Barrera-Figueroa, B.E., Gao, L., Diop, N.N., Wu, Z.G., Ehlers, J.D., Roberts, P.A., Close, T.J., Zhu, J.K. and Liu, R., 2011. Identification and comparative analysis of drought-associated microRNAs in two cowpea genotypes. BMC Plant Biology. 11: 127-135. - Bartel, D.P. and Chen, C.Z., 2004. Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs. Nature Reviews Genetics. 5: 396-400. - Chen, C., Ridzon, D.A., Broomer, A.J., Zhou, Z., Lee, D.H., Nguyen, J.T., Barbisin, M., Xu, N.L., Mahuvakar, V.R., Andersen, M.R., Lao, K.Q., Livak, K.J. and Guegler, K.J., 2005. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Research, 33: 179-186. - Chen, R., Ni, Z., Nie, X., Qin, Y., Dong, G. and Sun, Q., 2005. Isolation and characterization of genes encoding MYB transcription factor in wheat (Triticum aestivem L.). Plant Science, 169: 1146-1154. - Covarrubias, A.A. and Reyes, J.L., 2010. Post‐transcriptional gene regulation of salinity and drought responses by plant microRNAs. Plant, Cell & Environment, 33(4): 481-489. - Dugas, D.V. and Bartel, B. 2008. Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases. Plant Molecular Biology, 67:403-417. - Fabriki-Ourang, S. and Mehrabad Purbenab S., 2019. Evaluation of variations in physiological and biochemical traits in ancestral and evolutional species of wheat under water deficit stress. Environmental Stresses in Crop Sciences, 11(4): 791-802. - Fabriki-Ourang, S. and Shahidi B., 2019. Evaluation of genetic diversity effects on morpho-physiological and antioxidant responses in different species of Aegilops under drought stress. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 26(2): 254-267. - Filiz, E. and Tombuloglu, H., 2015. Genome-wide distribution of superoxide dismutase (SOD) gene families in Sorghum bicolor. Turkish Journal of Biology, 39: 49- 59. - Frazier, T., Sun, G., Burklew, C. and Zhang, B., 2011. Salt and drought stresses induce the aberrant expression of microRNA genes in tobacco. Molecular Biotechnology, 49:159-165. - Gubler, F., Chandler, P.M., White, R.G., Llewellyn, D.J. and Jacobsen, J.V., 2002. Gibberellin signaling in barley aleurone cells Control of SLN1 and GAMYB expression. Plant Physiology, 129: 191-200. - Hagen, G. and Guilfoyle, T., 2002. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Molecular Biology, 49: 373-385. - Ismaili, F., Shiran, B., Mirakhorli, N., Fallahi, H., 2015. Investigation of miR159 and miR171 expression pattern under drought stress in peaches, almonds and GN. Modern Genetics, 10: 416-407. - Jagadeeswaran, G., Saini, A. and Sunkar, R., 2009. Biotic and abiotic stress down-regulate miR398 expression in Arabidopsis. Planta, 229: 1009-1014. - Jones-Rhoades, M.W. And Bartel, D.P., 2004. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Molecular Cell, 14: 787-799. - Kantar, M., Lucas, S.J. and Budak, H., 2011. MiRNA expression patterns of Triticum dicoccoides in response to shock drought stress. Planta, 233: 471-484. - Kebeish, R., Hanan, E. and El-Bialy, N., 2015. Effects of gamma radiation on growth, oxidative stress, antioxidant system, and alliin producing gene transcripts in Allium sativum. International Journal of Research Studies in Bioscience, 3:161-174. - Kohli, A., Sreenivasulu, N., Lakshmanan, P. and Kumar, PP., 2013. The phytohormone crosstalk paradigm takes center stage in understanding how plants respond to abiotic stresses. Plant cell reports, 1-13. - Lee, H., Yoo, S.J., Lee, J., Kim, W., Yoo, S.K., Fitzgerald, H., Carrington, J.C. and Ahn, J.H., 2010. Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis. Nucleic Acids Research, 38: 3081-3093. - Li, J.S., Fu, F.L., An, M., Zhou, S.F., She, Y.H. and Li, W.C., 2013. Differential expression of microRNAs in response to drought stress in maize. Journal of Integrative Agriculture, 12:1414-1422. - Li, L. and Huilan. Y., 2012. Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsis plants. Plant Physiology and Biochemistry, 58:46-53. - Liu, Q. and Chen, Y.Q., 2009. Insights into the mechanism of plant development: interactions of miRNAs pathway with phytohormone response. Biochemical and Biophysical Research Communications, 384: 1-5. - Lu, S.F., Sun, Y.H. and Chiang, V.L., 2008. Stress-responsive microRNAs in Populus. Plant Journal,55:131-151. - Lu, W., Li, J., Liu, F., Gu, J., Guo, C., Xu, L., Zhang, H. and Xiao, K., 2011. Expression pattern of wheat miRNAs under salinity stress and prediction of salt inducible miRNAs targets. Frontiers of Agriculture in China, 1-10. - Mallory, A.C., Bartel, D.P. and Bartel, B., 2005. MicroRNA-directed regulation of Arabidopsis auxin response factor17 is essential for proper development and modulates expression of early auxin response genes. The Plant Cell Online, 17: 1360-1375. - Mittler, R. and Blumwald, E., 2010. Genetic engineering for modern agriculture: Challenges and perspectives. Annu. Rev. Plant Biology, 61: 443-462. - Phillips, J.R., Dalmay, T. and Bartels, D., 2007. The role of small RNAs in abiotic stress.FEBS Letters,581(19): 3592-3597. - Qu, C.P., Xu, Z.R., Liu, G.J., Liu, C., Li, Y., Wei, Z.G. and Liu, G.F., 2010. Differential expression of copper-zinc superoxide dismutase gene of Polygonum sibiricum leaves, stems, and underground stems, subjected to high salt stress. International Journal of Molecular Science, 11: 5234–5245. - Reyes, J.L. and Chua, N.H., 2007. ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. The Plant Journal, 49:592-606. - Rhoades, M.W., Reinhart, B.J., Lim, L.P., Burge, C.B., Bartel, B. and Bartel, D.P., 2002. Prediction of plant microRNA targets. Cell, 110: 513-520. - Safarzadeh, M., Fototo, R., Azimi, M.R., Mohseni Fard, A. and Bakhshi, B., 2014. Investigation of expression of miRNAs controlling transcription factors associated with signaling of auxin, gibberellin and abscisic acid under drought stress conditions in wheat (Triticum aestivum L.). Biotechnology of Crop Plants, 6: 21-33. - Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli M., 2012. Reactive oxygen species, oxidative damage and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 21: 70-76. - Shinozaki, K. and Yamaguchi-Shinozaki K., 2007. Gene networks involved in drought stress response and tolerance. Journal of experimental botany, 58(2): 221-227. - Shukla, LI., Chinnusamy, V. and Sunkar, R., 2008. The role of microRNAs and other endogenous small RNAs in plant stress responses. Biochimica and Biophysica Acta (BBA)-Gene Regulatory Mechanisms. 1779(11): 743-748. - Sunkar, R. Li, Y.F. and Jagadeeswaran, G., 2012. Functions of microRNAs in plant stress responses. Trends in Plant Science, 17:196-203. - Sunkar, R., 2010. MicroRNAs with macro effects on plant stress responses. Seminars in Cell and Developmental Biology, 21: 805-811. - Sunkar, R., Kapoor, A. and Zhu, J.K., 2006. Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by down-regulation of miR398 and important for oxidative stress tolerance. Plant Cell, 18: 2051-2065. - Tang, Z., Zhang, L., Xu, C., Yuan, S., Zhang,F., Zheng, Y. and Zhao, C., 2012. Uncovering small RNA-mediated responses to cold stress in a wheat thermo-sensitive genic male-sterile line by deep sequencing. Plant Physiology, 159(2): 721-738. - Trindade, I., Capitao, C., Dalmay, T., Fevereiro, M. and Santos, D., 2010. MiR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula. Planta, 231:705–716. - Tuteja, N., 2007. Abscisic acid and abiotic stress signaling. Plant Signaling & Behavior, 2: 135-138. - Umezawa, T., Fujita, M., Fujita, Y., Yamaguchi, S.K. and Shinozaki, K., 2006. Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. Science Direct, 17: 113-122. - Wang, B., Sun, Y., Song, N., Wei, J.P., Wang, X.J., Feng, H. and Yin, Z.Y., 2014. MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum. Plant Physiology and Biochemistry, 80: 90-96. - Xin, M., Wang, Y., Yao, Y., Xie, C., Peng, H., Ni, Z. and Sun, Q., 2010. Diverse set of microRNAs are responsive to powdery mildew infection and heat stress in wheat (Triticum aestivum L.). BMC Plant Biology, 10: 123-129. - Yu, X., Wang, H., Lu, Y., De-Ruiter, M., Cariaso, M., Prins, M.V., Tunen, A. and He, Y., 2012. Identification of conserved and novel microRNAs those are responsive to heat stress in Brassica rapa. Journal of Experimental Botany, 63: 1025-1038. - Zhou, L.G., Liu, Y.H., Liu, Z.C., Kong, D.Y., Duan, M. and Luo, L.J., 2010. Genome-wide identification and analysis of drought-responsive microRNAs in Oryza sativa. Journal of Experimental Botany, 61: 4157-4168. | ||
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آمار تعداد مشاهده مقاله: 468 تعداد دریافت فایل اصل مقاله: 485 |
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