Effect of 2, 4-Dinitrophenol on reactive oxygen species production in mitochondria and improvement of sperm viability after freeze-thawing process of Rooster semen | ||
| علوم دامی | ||
| Article 1, Volume 32, Issue 123, September 2019, Pages 3-16 PDF (1.12 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/asj.2018.121379.1674 | ||
| Authors | ||
| Nahid Mohammadi1; Hossein Daghigh Kia* 2 | ||
| 1Department of Animal Science, Faculty of Agriculture, University of Tabriz | ||
| 2University of Tabriz | ||
| Abstract | ||
| Regarding the role of mitochondria in the quality of sperm motility and fertility, protection of mitochondrial function against ROS-induced damage can be an important step in improving roster sperm fertility. The mitochondria are the most important source of ROS and the unique properties of mitochondria prevent antioxidants from entering, which results in poor antioxidant performance in protecting ROS-induced damage. Therefore, the use of antioxidants that can pass through the mitochondrial membrane and inhibit ROS in this region can be a good way to reduce the damage caused by ROS and improve the sperm quality and fertility. The aim of the present study was to use mitochondrial mild uncoupling combinations to reduce the risk of ROS-induced injuries and increase the survival of sperm after freeze-thawing. For this purpose, 14 Ross breed rooster with 28 weeks of age were used. The semen was taken by massaging the abdomen-back procedure. After initial evaluation, semen samples were pooled After dilution of semen samples and addition of 2-4-dinitrophenol (DNP) at five levels (0, 0.25, 0.5, 0.75, 1 nM), they were cooled and then frozen. After two months, semen samples were thawed and evaluated for the percentage of dead and live sperm, sperm motility parameters, membrane integrity, lipid peroxidation and sperm morphology. The results of this study showed that addition of DNP at 0.75 nM resulted in improvement in total and progressive motility, viability, plasma membrane integrity and sperm normality compared to the control group, and significantly decreased MDA concentration compared to control group (p <0.05). | ||
| Keywords | ||
| Mitochondria; Mitochondrial mild uncoupling; Reactive oxygen species | ||
| References | ||
|
Amaral, A., Lourenco, B., Marques, M. and Ramalho-Santos, J. (2013). Mitochondria functionality and sperm quality. Reproduction, 146: 163-174. Amaral, A., Paiva, C., Baptista, M., Sousa, AP. and Ramalho-Santos, J. (2011). Exogenous glucose improves long-standing human sperm motility, viability, and mitochondrial function. Fertility and Sterility, 96: 848–850. Antonenko, Y.N., Avetisyan, A.V., Bakeeva, L.E., Chernyak, B.V., Chertkov, V.A., Domnina, L.V et al. (2008). Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of an aging program. 1. Cationic plastoquinone derivatives: synthesis and in vitro studies. Biochemistry, 73: 1273–1287. Azzu, V. and Brand, M.D. 2010. The on-off switches of the mitochondrial uncoupling proteins. Trends in Biochemical Sciences, 35: 298-307. Akhlaghi A, Jafari Y, Zhandi M and Peebles ED. (2014). Reproductive performance, semen quality, and fatty acide profile of spermatozoa in senescent broiler breed rooster as enhanced by the long-term feeding of dried apple pomace. Animal Reproduction Science. 147: 64-73 Baffy, G. (2010). Uncoupling protein-2 and cancer. Mitochondrion, 10:243–252 Bakhshayesh Khiabani, A., Moghaddam, G. and Daghigh Kia, H. (2017). Effects of adding different levels of Glutamine to modified Beltsville extender on the survival of frozen rooster semen. Animal Reproduction Science, 184: 172–177 Balercia G, Gandini L, and Lenzi A. (2017). Antioxidants in andrology. Thends in Andrology and sexual medicin. Gewerbestrasse 11, 6330 Cham, Switzerland. Choi, S.Y., Gonzalvez, F., Jenkins, G.M., Slomianny, C., Chretien, C., Arnoult, D., et al. (2007). Cardiolipin deficiency releases cytochrome c from the inner mitochondrial membrane and accelerates stimuli-elicited apoptosis. Cell Death and Differentiation, 14: 597–606. Dong, Q., Tollner, T.L., Rodenburg, S.E., Hill, D.L. and VandeVoort, C.A. (2010). Antioxidants, oxyrase, and mitochondrial uncoupler 2, 4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival. Fertility and Sterility, 94: 2359-2361. Echtay, K.S. (2007). Mitochondrial uncoupling proteins—What is their physiological role? Free Radical Biology and Medicine, 43: 1351-1371. Echtay, K.S. and Brand, M.D. (2007). 4-hydroxy-2-nonenal and uncoupling proteins: an approach for regulation of mitochondrial ROS production. Redox Report, 12: 26-29. Fang, L., Bai, C., Chen,Y., Dai, J., Xiang, Y., Ji, X et al. (2014). Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish. Cryobiology, 69: 386-393 Ferramosca, A. (2014). Bioenergetics of mammalian sperm capacitation. Biomed Resarch International, 1-8. Ford, W.C.L. (2006). Glycolysis and sperm motility: does a spoonful of sugar help the flagellum go round? Human Reproduction Update, 12(3): 269–274 Frederick, S. (2010). Hep G2Hepatocyte Lipid Peroxidation Assay. NCL Method GTA- 4. Version1.1. Hansford, R.G., Hogue, B.A. and Mildaziene, V.J. (1997). Dependence of H2O2 formation by rat heart mitochondria on substrate availability and donor age. Journal of Bioenergetics and Biomembranes, 29: 89-95 Harper, JA., Dickinson, K. and Brand, M.D. (2001). Mitochondrial uncoupling as a target for drug development for the treatment of obesity. Obesity Reviews, 2:255-65. Herrero, A. and Barja, G. (1998). H2O2 production of heart mitochondria and aging rate are slower in canaries and parakeets than in mice: sites of free radical generation and mechanisms involved. mech. Mechanisms of Ageing and Development, 103 133-146. Hirst, J., King, MS. and Pryde, K.R. (2008). The production of reactive oxygen species by complexI. Biochemical Society Transactions, 36: 976-980. Hoffman, D.L., Salter, J.D. and Brookes, P.S. (2007). Response of mitochondrial reactive oxygen species generation to steady-state oxygen tension: implications for hypoxic cell signaling. American Journal of Physiology Heart and Circulatory, 292: 101-108. Karim, S. and Echtay, M.D. (2007). 4-Hydroxy-2-nonenal and uncoupling proteins: an approach for regulation of mitochondrial ROS production. W. S. Maney and Son Ltd, 12: 26-29. Kudin, A.P., Bimpong-Buta, N.Y., Vielhaber, S., Elger, C.E. and Kunz,W.S. (2004). Characterization of superoxide-producing sites in isolated brain mitochondria. Journal of Biological Chemistry, 279: 4127- 4135. Lanning, L.L., Creasy, D.M., Chapin, R.E., Mann, P.C., Barlow, N.J., Regan, K.S et al. (2002). Recommended approaches for the evaluation of testicular and epididymal toxicity. Toxicologic Pathology, 30, 507-520. Lardy, H.A. and Phillips, P.H. (1945). Studies of fat and carbohydrate oxidation in mammalian spermatozoa. Archives of Biochemistry and Biophysics, 6: 53-61. Lee, S.C., Robson-Doucette, C.A. and Wheeler, M.B. (2009). Uncoupling protein 2 regulates reactive oxygen species formation in islets and influences susceptibility to diabetogenic action of streptozotocin. Journal of Endocrinology, 203:33–43. Machaty, Z., Thompson, J.G., Abeydeera, L.R., Day, B.N. and Prather, R.S. (2001). Inhibitors of mitochondrial ATP production at the time of compaction improve development of in vitro produced porcine embryos. Molecular Reproduction and Development, 58: 39-44. Madeddu, M., Mosca, F., Abdel Sayed, A., Zaniboni, L., Mangiagalli, MG., Colombo, E et al. (2016). Effect of cooling rate on the survival of cryopreserved rooster sperm: Comparison of different distances in the vapor above the surface of the liquid nitrogen. Animal Reproduction Science, 171: 58-64. Mailloux, R.J. and Harper, M.E. (2011). Uncoupling proteins and the control of mitochondrial reactive oxygen species production. Free Radical Biology and Medicine, 51:1106–1115. Mirzaei Rad, H., Eslami, M. and Ghanie, A. (2016). Palmitoleate enhances quality of rooster semen during chilled storage. Animal Reproduction Science, 165: 38–45. Mphaphathi, M.L., Seshoka, M.M., Luseba, D., Sutherland, B. and Nedambale, T.L. (2016). The characterisation and cryopreservation of Venda chicken semen. Asian Pacific Journal of Reproduction, 5: 132–139 Mukai, C. and Okuno, M. (2004). Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biology of Reproduction, 71:540–547. Mukhopadhyay, A. and Weiner, H. (2007). Delivery of drugs and macromolecules to mitochondria. Advanced Drug Delivery Reviews, 59: 729–738. Murphy, M.P. and Smith, R.A.J. (2000). Drug delivery to mitochondria: the key to mitochondrial medicine. Advanced Drug Delivery Reviews, 41: 235–250. Rousset, R., Alves-Guerra, M.C., Mozo, J., Miroux, B., Cassard-Doulcier, A.M., Bouillaud, F.R et al. (2004). The Biology of Mitochondrial Uncoupling ProteinS. Diabetes, 53(1): 130-135. Rui, B.R., Angrimani, D.S.R., Losano, J.D.A., Bicudo, L.D.C., Nichi, M., and Pereira, R.J.G. (2017). Validation of simple and cost-effective stains to assess acrosomal status, DNA damage and mitochondrial activity in rooster spermatozoa. Animal Reproduction Science, 187: 133–140. Safa, S., Moghaddam, G., Jozani, R.J., Daghigh Kia, H. and Janmohammadi, H. (2016). Effect of vitamin E and selenium nanoparticles on post-thaw variables and oxidative status of rooster semen. Animal Reproduction Science, 174, 100–106. Shabalina, O.N., Shekarova, M.V., Skulachev, T.V., Titova, V.A., Vygodin, M.Y., Vyssokikh, M.N et al. (2011). Effects of the mitochondria-targeted antioxidant SkQ1 on lifespan of rodents. Aging (Albany), 3: 1110–1119 Silva, E.F., Junior, A.S.V., Cardoso, T.F., Stefanello, F.M., Kalb, A.C., Martínez, P.E et al. (2016). Reproductive toxicology of 2,4 dinitrophenol in boar sperm. Toxicology in Vitro, 35: 31–35. Skulachev, V.P., Anisimov, V.N., Antonenko, Y.N., Bakeeva, L.E., Chernyak, B.V., Erichev, V.P et al. (2009). An attempt to prevent senescence: A mitochondrial approach. Biochimica et Biophysica Acta, 1787: 437–461. Smith, R.A. and Murphy, M.P. (2011). Mitochondria-targeted antioxidants as therapies. Discovery medicine, 11: 106–114. Thompson, J., McNaughton, C., Gasparrini, B. and McGowan, L. (2000). Effect of inhibitors and uncouplers of oxidative phosphorylation during compaction and blastulation of bovine embryos cultured in vitro. Reproduction Fertility, 118: 47-55. Venditti, P., Di Stefano, L. and Di Meo, S. (2013). Mitochondrial metabolism of reactive oxygen species. Mitochondrion, 13: 71-82. Wang, G., Kang, N., Gong, H., Luo, Y., Bai, C., Chen, Y.J.X et al. (2015). Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish. Cryobiology, 71: 464-471 Wojtczak, L., Lebiedzi_nska, J.M., Suski, M.R. and Wie ckowski, P. (2011). Sch€onfeld, Inhibition by purine nucleotides of the release of reactive oxygen species from muscle mitochondria: indication for a function of uncoupling proteins as superoxide anion transporters. Biochemical and Biophysical Research Communications, 407: 772-776. | ||
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