News

a.z
Asadi, N., Lotfi, M., Harzandi, N. (2019). Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method. , 32(4), 29-37. doi: 10.22092/vj.2019.124320.1529
Nahid Asadi; Mohsen Lotfi; Naser Harzandi. "Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method". , 32, 4, 2019, 29-37. doi: 10.22092/vj.2019.124320.1529
Asadi, N., Lotfi, M., Harzandi, N. (2019). 'Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method', , 32(4), pp. 29-37. doi: 10.22092/vj.2019.124320.1529
Asadi, N., Lotfi, M., Harzandi, N. Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method. , 2019; 32(4): 29-37. doi: 10.22092/vj.2019.124320.1529

Investigation of Nanobacteria contamination in cell substrate used in biological productions in Razi Institute by molecular method

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 4, Volume 32, Issue 4, December 2019, Pages 29-37    PDF (2.51 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.124320.1529
Authors
Nahid Asadi1; Mohsen Lotfi* 2; Naser Harzandi1
1Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
Abstract
Production of viral vaccines and also drugs products which are derive from cells produced in various biological materials, like cell substrates eg: fertile eggs, primary cells, cell lines, raw materials and additives used in culture media which have animal origin and seed virus which is used for culture have a complicated process. The above processes are naturally vulnerable for contamination to adventitious agents. The Nanobacteria like other adventitious agents can cause contamination through primary materials with animal origin for such a product. The aim of this study was to design a program for PCR which is specific for detection of probable presence of Nanobacteria in cells, especially in cell lines and diploids which is used in vaccine production in Razi institute. For this purpose the cells which are used in following departments, research, production and Quality control for biological products were collected and DNA was extracted for PCR expression on 16S rRNA area from Nanobacteria. Bartonella henselae was used as a positive control. As it was expected the results of PCR products after electrophoresis were positive for above organism and at the area of 558 bp replicated which it confirms the correctness of our experiment. The cells from all departments were negative for Nanobacteria, however for not having enough information for spread and risk evaluation of Nanobacteria in cattle population there are a great need for further investigation. 
Keywords
Nanobacterium sanguineum; Cell culture; Vaccine; Adventitious agents; PCR
References

1. Aho, K and E. O. Kajander. 2003. Pitfalls in Detection of Novel Nanoorganisms. Journal of clinical microbology 41(7): 0095-1137.
2. Ansari H., A. Akhavan Sepahi and M. Akhavan Sepahi. 2017. Different Approaches to Detect “Nanobacteria” in Patients with Kidney Stones: an Infectious Cause or a Subset of Life. Endourology and stone disease journal 14 (5):5001-5007.
3. Barr S. C., R. A. Linke, D. Janssen, C. L. Guard, M.C. Smith, C. S. Daugherty and J. M. Scarlett. 2003. Detection of biofilm formation and Nanobacteria under long-term cell culture conditions in serum samples of cattle, goats, cats, and dogs. American journal of veterinary research 64(2): 176-182.
4. Ciftcioglu, N and E. O. Kajander. 1998. Interaction of Nanobacteria with cultured mammalian cells. Pathophysiology journal 4(4):259-270.
5. Drancourt, M., V. Jacomo, H. Lepidi, E. Lechevallier, V. Grisoni, C. Coulange and E. Ragni. 2003. Attempted isolation of Nanobacterium sp. Microorganisms from upper urinary tract stones. Journal of clinical microbiology 41:368–372.
6. Furtak, V. A., A. Dabrazhynetskaya, D. V. Volokhov and V. Chizhikov. 2015. Use of tangential flow filtration for improving detection of viral adventitious agents in cell substrates. Biologicals journal 43(1):23-30.
7. Habibipour, R., G. R. Zarrini and S.H. Yarizadeh. 2017. Isolation and Characterization of Hyperthermophilic Nanobacteria from a Hot Spring in Ardabil, Iran. Medical laboratory journal 11(3):20-24.
8. Izadi, A and E. Moslemi. 2014. A Survey on the Presence of Calcifying Nanoparticles in Renal Stones, Gallbladder Stones and Atherosclerosis Plaque. Journal of kerman university of medical sciences 21(4): 302-312. (In Farsi).
9. Jerman, I. 2017. What Nanobacteria and Nanovesicles May Tell Us about the Origin of Life. Open access library journal 4: e3348.
10. Kolahi, J., M. Shahmoradi and M. Sadreshkevary. 2018. Nanobacteria in clouds can spread oral pathologic calcifications around the world. Dental hypotheses journal 3(4):138-141.
11. Lu, H., Y. N. Guo, S. N. Liu, H. Zhu and D. C. Zhang. 2012. Isolation, cultivation and identification of Nanobacteria from placental calcification. The Journal of Maternal-Fetal and Neonatal Medicine 25(11): 2182–2185.
12. Lwin, K. M. 2012. Nanobacterium sanguineum In Pathological Calcifications. first printing. FAME Publishing House, Hlaing Tharyar city of Industry Yangon Myanmar.
13. Martel, J., H. H. Peng, D. Young, C. Y. Wu and J. D. Young. 2014. Of Nanobacteria, nanoparticles, biofilms and their role in health and disease: facts, fancy and future. Future Medicine Ltd 9(4), 483–499.
14. Miller, V. M., G. Rodgers and J. A. Charlesworth. 2004. Evidence of Nanobacterial-like structures in calcified human arteries and cardiac valves. American Journal of Physiology-Heart and Circulatory Physiology 287(3): 1115–1124.
15. Petricciani, J., R. Sheets, E. Griffiths and I. Knezevic. 2014. Adventitious agents in viral vaccines: Lessons learned from 4 case studies. Biologicals journal 42(5):223-36.
16. Sardarabadi, H., M. Mashreghi, K. h. Jamialahmadi and T. Dianat. 2014. Resistance of Nanobacteria isolated from urinary and kidney stones to broad-spectrum antibiotics. Iranian journal of microbiology 6(4):230-233.
17. Shojaeian, A., M. Rostamian, J. Noroozi and P. Pakzad. 2016. The Identification of Chemical and Bacterial Composition and Determination of FimH Gene Frequency of Kidney Stones of Iranian Patients. Zahedan journal of research in medical sciences 18(6):e7363.
18. WHO. 2013. Recommendations for the evaluation of animal cell cultures as substrates for the manufacture of biological medicinal products and for the characterization of cell banks. Technical Report Series, No. 978. available online at: http://www.who.int/biologicals/Cell_Substrates_clean_version_18_April.pdf
19. Yaghobee, S., M. Bayani, N. Samiei and N. Jahedmanesh. 2015. Review; What are the Nanobacteria. Biotechnology & Biotechnological Equipment 29 (5): 826–833.

Statistics
Article View: 774
PDF Download: 621


counter
Journal management system. designed by sinaweb