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استفاده از روش سطح پاسخ بهمنظور بهینهسازی استخراج عصاره بخش هوایی کرفس (Apium graveolens L.) و ترکیبهای فنولیک و فعالیت آنتیاکسیدانی آن به کمک امواج فراصوت | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 35، شماره 4 - شماره پیاپی 96، مهر و آبان 1398، صفحه 644-664 اصل مقاله (1.44 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.124004.2431 | ||
| نویسندگان | ||
| فاطمه سلیمی* 1؛ محمد فتاحی2؛ جواد حمزه ئی3 | ||
| 1دانشآموخته دکترای تخصصی، گروه باغبانی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استادیار، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3دانشیار، دانشگاه بوعلیسینا همدان، همدان، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.644.96.4.1575.1578 در این تحقیق، اثر زمان (10، 15، 20، 25 و 30 دقیقه) و دمای امواج فراصوت (35، 45، 55، 65 و 75 درجه سانتیگراد)، نسبت اتانول به آب (20، 35، 50، 65 و 80 درصد) و نسبت ماده خشک به حلال (5:1، 10:1، 15:1، 20:1 و 25:1 میلیگرم بر میلیلیتر) بر ویژگیهای فنول و فلاونوئید کل و ظرفیت آنتیاکسیدانی عصاره هیدروالکلی حاصل از گیاه کرفس (Apium graveolens L.) بررسی شد. استخراج عصاره از بخش هوایی کرفس، با روش عصارهگیری هیدروالکلی به کمک امواج فراصوت با بهرهگیری از روش سطح پاسخ انجام گردید و صفات فنول کل، فلاونوئید کل و ظرفیت آنتیاکسیدانی به دو روش DPPH و FRAP اندازهگیری و ارزیابی گردید. براساس نتایج، اعمال زمان 10 دقیقه امواج فراصوت، 55 درجه سانتیگراد دمای امواج فراصوت، نسبت اتانول به آب 80% و نسبت ماده خشک به حلال 5:1 بهترین تیمار بود. در شرایط بهینه، مقدار فنول و فلاونوئید کل بهترتیب 471.12 میلیگرم گالیک اسید در یک گرم ماده خشک و 187.70 میلیگرم کوئرستین در یک گرم ماده خشک و میزان فعالیت آنتیاکسیدانی عصاره به روش DPPH و FRAP بهترتیب 68.48% و 4210.4 میکرومولار Fe+2 در 50 میکرولیتر عصاره بدست آمد. در کل، R2-Adjusted R2 و P-value بالا در برازش مدلها، بیانگر ایدهآل بودن مدلهای سطح پاسخ (RSM) ترسیم شده است. بهعبارت دیگر، بالا بودن این پارامترها نشانگر وجود همبستگی بالای بین مقادیر مشاهده شده و مقادیر پیشبینی شده میباشد. | ||
| کلیدواژهها | ||
| امواج فراصوت؛ روش RSM؛ عصاره؛ فعالیت آنتیاکسیدانی؛ فلاونوئید؛ فنول؛ گیاه دارویی | ||
| عنوان مقاله [English] | ||
| Application of response surface methodology to optimize celery (Apium graveolens L.) aerial part extraction and its phenolic compounds and antioxidant activity using ultrasound-assisted | ||
| نویسندگان [English] | ||
| F. Salimi1؛ M. Fattahi2؛ J. Hamzei3 | ||
| 1Horticulture Department, Urmia University, Urmia, Iran | ||
| 2Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Bu-Ali Sina University, Hamedan, Iran | ||
| چکیده [English] | ||
| In this research, the effect of time (10, 15, 20, 25 and 30 min) and temperature (35, 45, 55, 65 and 75 oC) of ultrasonic waves, ethanol to water ratio (20, 35, 50, 65 and 80%) of extracting solvent and dry matter to solvent ratio (1:5, 1:10, 1:15, 1:20 and 1:25 mg/ml) was investigated on total phenol and flavonoids and antioxidant capacity (in two methods of DPPH and FRAP) of hydroalcoholic extract of celery (Apium graveolens L.) aerial parts by using the response surface method. Based on the results, ultrasound for 10 min at 55 °C, ethanol to water ratio of 80%, and dry matter to solvent ratio of 1:5 were considered as the best treatment. The highest amount of total phenol (471.1 mg GAE/g DW) and flavonoid (187.7 mg quercetin/gDW) content, and antioxidant activity of the extract was obtained under optimized conditions using DPPH (68.5%) and FRAP (4210.4 μM Fe+2/50 μl extract). In general, the high values of adjusted R2 and P-value in fitting models indicated that the drawn models of response surface were ideal. In other words, the high amounts of these parameters indicate the existence of a correlation between the observed values and the predicted ones. | ||
| کلیدواژهها [English] | ||
| Ultrasound, response surface methodology, extract, antioxidant activity, flavonoid, phenol, medicinal plants | ||
| مراجع | ||
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