| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 3,086 |
| تعداد مقالات | 34,442 |
| تعداد مشاهده مقاله | 48,908,504 |
| تعداد دریافت فایل اصل مقاله | 79,348,346 |
استفاده از روش سطح پاسخ به‌منظور بهینه‌سازی استخراج عصاره بخش هوایی کرفس (Apium graveolens L.) و ترکیب‌های فنولیک و فعالیت آنتی‌اکسیدانی آن به کمک امواج فراصوت | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 35، شماره 4 - شماره پیاپی 96، مهر و آبان 1398، صفحه 644-664 اصل مقاله (1.44 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.124004.2431 | ||
| نویسندگان | ||
| فاطمه سلیمی* 1؛ محمد فتاحی2؛ جواد حمزه ئی3 | ||
| 1دانش‌آموخته دکترای تخصصی، گروه باغبانی، دانشگاه ارومیه، ارومیه، ایران | ||
| 2استادیار، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
| 3دانشیار، دانشگاه بوعلی‌سینا همدان، همدان، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.644.96.4.1575.1578 در این تحقیق، اثر زمان (10، 15، 20، 25 و 30 دقیقه) و دمای امواج فراصوت (35، 45، 55، 65 و 75 درجه سانتی‌گراد)، نسبت اتانول به آب (20، 35، 50، 65 و 80 درصد) و نسبت ماده خشک به حلال (5:1، 10:1، 15:1، 20:1 و 25:1 میلی‌گرم بر میلی‌لیتر) بر ویژگی‌های فنول و فلاونوئید کل و ظرفیت آنتی‌اکسیدانی عصاره هیدروالکلی حاصل از گیاه کرفس (Apium graveolens L.) بررسی شد. استخراج عصاره از بخش هوایی کرفس، با روش عصاره‌گیری هیدروالکلی به کمک امواج فراصوت با بهره‌گیری از روش سطح پاسخ انجام گردید و صفات فنول کل، فلاونوئید کل و ظرفیت آنتی‌اکسیدانی به دو روش DPPH و FRAP اندازه‌گیری و ارزیابی گردید. براساس نتایج، اعمال زمان 10 دقیقه امواج فراصوت، 55 درجه سانتی‌گراد دمای امواج فراصوت، نسبت اتانول به آب 80% و نسبت ماده خشک به حلال 5:1 بهترین تیمار بود. در شرایط بهینه، مقدار فنول و فلاونوئید کل به‌ترتیب 471.12 میلی‌گرم گالیک اسید در یک گرم ماده خشک و 187.70 میلی‌گرم کوئرستین در یک گرم ماده خشک و میزان فعالیت آنتی‌اکسیدانی عصاره به روش DPPH و FRAP به‌ترتیب 68.48% و 4210.4 میکرومولار Fe+2 در 50 میکرولیتر عصاره بدست آمد. در کل، R2-Adjusted R2 و P-value بالا در برازش مدل‌ها، بیانگر ایده‌آل بودن مدل‌های سطح پاسخ (RSM) ترسیم شده است. به‌عبارت دیگر، بالا بودن این پارامترها نشانگر وجود همبستگی بالای بین مقادیر مشاهده شده و مقادیر پیش‌بینی شده می‌باشد. | ||
| کلیدواژه‌ها | ||
| امواج فراصوت؛ روش RSM؛ عصاره؛ فعالیت آنتی‌اکسیدانی؛ فلاونوئید؛ فنول؛ گیاه دارویی | ||
| عنوان مقاله [English] | ||
| Application of response surface methodology to optimize celery (Apium graveolens L.) aerial part extraction and its phenolic compounds and antioxidant activity using ultrasound-assisted | ||
| نویسندگان [English] | ||
| F. Salimi1؛ M. Fattahi2؛ J. Hamzei3 | ||
| 1Horticulture Department, Urmia University, Urmia, Iran | ||
| 2Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| 3Bu-Ali Sina University, Hamedan, Iran | ||
| چکیده [English] | ||
| In this research, the effect of time (10, 15, 20, 25 and 30 min) and temperature (35, 45, 55, 65 and 75 oC) of ultrasonic waves, ethanol to water ratio (20, 35, 50, 65 and 80%) of extracting solvent and dry matter to solvent ratio (1:5, 1:10, 1:15, 1:20 and 1:25 mg/ml) was investigated on total phenol and flavonoids and antioxidant capacity (in two methods of DPPH and FRAP) of hydroalcoholic extract of celery (Apium graveolens L.) aerial parts by using the response surface method. Based on the results, ultrasound for 10 min at 55 °C, ethanol to water ratio of 80%, and dry matter to solvent ratio of 1:5 were considered as the best treatment. The highest amount of total phenol (471.1 mg GAE/g DW) and flavonoid (187.7 mg quercetin/gDW) content, and antioxidant activity of the extract was obtained under optimized conditions using DPPH (68.5%) and FRAP (4210.4 μM Fe+2/50 μl extract). In general, the high values of adjusted R2 and P-value in fitting models indicated that the drawn models of response surface were ideal. In other words, the high amounts of these parameters indicate the existence of a correlation between the observed values and the predicted ones. | ||
| کلیدواژه‌ها [English] | ||
| Ultrasound, response surface methodology, extract, antioxidant activity, flavonoid, phenol, medicinal plants | ||
| مراجع | ||
|
- Amyrgialaki, E., Makris, D.P., Mauromoustakos, A. and Kefalas, P., 2014. Optimisation of the extraction of pomegranate (Punica granatum) husk phenolics using water/ethanol solvent systems and response surface methodology. Industrial Crops and Products, 59: 216-222. - Araujo, P.W. and Brereton, R.G., 1996. Experimental design I. Screening. TrAC Trends in Analytical Chemistry, 15(1): 26-31. - Benzie, I.F. and Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 15: 239(1): 70-76. - Bezerra, M.A., Santelli, R.E., Oliveira, E.P., Villar, L.S. and Escaleira, L.A., 2008. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, 76(5): 965-977. - Carrera, C., Ruiz-Rodríguez, A., Palma, M. and Barroso, C.G., 2012. Ultrasound assisted extraction of phenolic compounds from grapes. Analytica Mhimica Acta, 732: 100-104. - Chavan, Y. and Singhal, R.S., 2013. Ultrasound-assisted extraction (UAE) of bioactives from arecanut (Areca catechu L.) and optimization study using response surface methodology. Innovative Food Science and Emerging Technologies, 17: - Chen, W., Wang, W.P., Zhang, H.S. and Huang, Q., 2012. Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletus edulis mycelia using response surface methodology. Carbohydrate Polymers, 87(1): 614-619. - Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S. and Ju, Y.H., 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3): 296-302. - Farizadeh, K., Montazer, M., Yazdanshenas, M.E., Rashidi, A. and Malek, R.M.A., 2009. Extraction, identification and sorption studies of dyes from madder on wool. Journal of Applied Polymer Science, 113(6): 3799-3808. - Ghasemzadeh, A., Jaafar, H.Z. and Karimi, E., 2012. Involvement of salicylic acid on antioxidant and anticancer properties, anthocyanin production and chalcone synthase activity in ginger (Zingiber officinale Roscoe) varieties. International Journal of Molecular Sciences, 13(11): 14828-14844. - Ghasemzadeh, A., Jaafar, H.Z., Karimi, E. and Rahmat, A., 2014. Optimization of ultrasound- - Gupta, A., Naraniwal, M. and Kothari, V., 2012. Modern extraction methods for preparation of bioactive plant extracts. International Journal of Applied and Natural Science, 1: 8-26. - Hossain, M.B., Brunton, N.P., Patras, A., Tiwari, B., O’Donnell, C.P., Martin-Diana, A.B. and Barry-Ryan, C., 2012. Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology. Ultrasonics Sonochemistry, 19: 582-590. - Huang, W., Xue, A., Niu, H., Jia, Z. and Wang, J., 2009. Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro. Food Chemistry, 114(3): 1147-1154. - Ilghami, A., Ghanbarzadeh, S. and Hamishehkar, H., 2015. Optimization of the ultrasonic-assisted extraction of phenolic compounds, ferric reducing activity and antioxidant activity of the Beta vulgaris using response surface methodology. Pharmaceutical Sciences, 21(1): 46-50. - Kirby, A.J. and Schmidt, R.J., 1997. The antioxidant activity of Chinese herbs for eczema and of placebo herbs. Journal of Ethnopharmacology, 56(2): 103-108. - Kooti, W. and Daraei, N., 2017. A review of the antioxidant activity of celery (Apium graveolens L). Journal of Evidence-Based Complementary & Alternative Medicine, 22(4): 1029-1034. - Kooti, W., Ali-Akbari, S., Asadi-Samani, M., Ghadery, H. and Ashtary-Larky, D., 2014. A review on medicinal plant of Apium graveolens. Advanced Herbal Medicine, 1(1): 48-59. - Maran, J.P. Manikandan, S. and Mekala, V., 2013. Modeling and optimization of betalain extraction from Opuntia ficus-indica using Box-Behnken design with desirability function. Industrial Crops and Products, 49: 304-311. - Oancea, S., Stoia, M. and Coman, D., 2012. Effect of extraction conditions on bioactive anthocyanin content of Vaccinium corymbosum in the perspective of food applications. Procedia Engineering, 42: 489-495. - Şahin, S. and Şaml, R., 2013. Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology. Ultrasonics Sonochemistry, 20(1): 595-602. - Shabbir, M., Khan, M.R. and Saeed, N., 2013. Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leave. BMC Complementary and Alternative Medicine, 13(143): 13p. - Shin, Y., Liu, R.H., Nock, J.F., Holliday, D. and Watkins, C.B., 2007. Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and flavonoid concentrations, and antioxidant activity of strawberry. Postharvest Biology and Technology, 45(3): 349-357. - Shotipruk, A., Kaufman, B. and Wang, Y., 2001. Feasibility study of repeated harvesting of menthol from biologically viable menthe xpiperata using ultrasonic extraction. Biotechnology Progress, 17(5): 924-928. - Slinkard, K. and Singleton, V.L., 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1): 49-55. - Sowbhagya, H.B., 2014. Chemistry, technology, and nutraceutical functions of Celery (Apium graveolens L.): An overview. Critical Reviews in Food Science and Nutrition, 54: 389-398. - Spigno, G. and De Faveri, D.M., 2009. Microwave-assisted extraction of tea phenols: A phenomenological study. Journal of Food Engineering, 93(2): 210-217. - Swami, H.S., Singh, K.S.P., Gennaro, L. and Dev Dutt, R., 2008. Extraction Technologies for Medicinal and Aromatic Plants. International Centre for Science and High Technology Press, 260p. - Tavakoli Saberi, M.R. and Sedaghat, M.R., 2005. Medicinal Plants. (Translation). Golshan Publishing House, Tehran, 264p. - Tian, Y., Xu, Z., Zheng, B. and Lo, Y.M., 2013. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrasonics Sonochemistry, 20(1): 202-208. - Upadhyay, R., Nachiappan, G. and Mishra, H.N., 2015. Ultrasound-assisted extraction of flavonoids and phenolic compounds from Ocimum tenuiflorum leaves. Food Science and Biotechnology, 24(6): 1951-1958. - Wang, J., Sun, B., Cao, Y., Tian, Y. and Li, X., 2008. Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chemistry, 106: 804-810. - Wang, L. and Weller, C.L., 2006. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology, 17(6): 300-312. - Yang, Y.C., Wei, M.C., Huang, T.C., Lee, S.Z. and Lin, S.S., 2013. Comparison of modified ultrasound-assisted and traditional extraction methods for the extraction of baicalin and baicalein from Radix Scutellariae. Industrial Crops and Products, 45: 182-190. - Yaniv, Z., 2005. Handbook of Medicinal Plant. New York, London & Oxford: Food Product, Haworth Medical & Imprints of the Haworth Press, 365p. - Zeng, Z.L., Xi, G.X. and Ge, X.H., 2013. Study on comparison of two extraction methods of polysaccharide from Momordica charantia L. vines. Science and Technology of Food Industry, 34: 285-308. - Zhao, Y., Hou, Y., Tang, G., Cai, E., Liu, S., Yang, H. and Wang, S., 2014. Optimization of ultrasonic extraction of phenolic compounds from Epimedium brevicornum maxim using response surface methodology and evaluation of its antioxidant activities in vitro. Journal of Analytical Methods in Chemistry, 2014: 7p. - Zhou, J., Zheng, X., Yang, Q., Liang, Z., Li, D., Yang, X. and Xu, J., 2013. Optimization of ultrasonic-assisted extraction and radical-scavenging capacity of phenols and flavonoids from Clerodendrum cyrtophyllum Turcz leaves. PLOS One, 8(7): 1-8. - Zou, T.B., Wang, M., Gan, R.Y. and Ling, W.H., 2011. Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology. International Journal of Molecular Sciences, 12: 3006-3017. | ||
|
آمار تعداد مشاهده مقاله: 1,064 تعداد دریافت فایل اصل مقاله: 768 |
||