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اثر عصاره هیدروالکلی سرخهولیک (Crataegus monogyna Jacq.) بر هیستوپاتولوژی و نشانگرهای آسیب کبدی در موشهای صحرایی دیابتی نر بالغ | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 35، شماره 4 - شماره پیاپی 96، مهر و آبان 1398، صفحه 539-550 اصل مقاله (609.96 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.123484.2404 | ||
| نویسندگان | ||
| میثم احمدی نسب1؛ سعید ولیپور چهارده چریک* 2؛ محبوبه سترکی3 | ||
| 1کارشناس ارشد، گروه زیستشناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران | ||
| 2استادیار، گروه زیستشناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران | ||
| 3دانشیار، گروه زیستشناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.539.96.4.1575.1594 بیماری دیابت ملیتوس با تغییرات فیزیکی، فیزیولوژیکی و پاتولوژیکی در بافت کبد همراه است. هدف از این مطالعه بررسی اثرهای حفاظتی عصاره هیدروالکلی سرخهولیک (Crataegus monogyna Jacq.) بر هیستوپاتولوژی و عملکرد کبد در موشهای صحرایی دیابتی شده توسط استرپتوزوتوسین بود. در این مطالعه تجربی موشهای صحرایی نر نژاد wistar بهطور تصادفی در 6 گروه 8 تایی (8n=) شامل کنترل، دیابتی درمان نشده، دیابتی درمان شده با دوزهای 100، 200 و 400 (mg/kg) عصاره سرخهولیک و کنترل مثبت (دیابتی درمان شده با کاپتوپریل) تقسیم شدند. پس از 3 هفته تجویز عصاره و دارو بهصورت درون صفاقی، سطح سرمی آنزیمهای کبدی و آلبومین تعیین گردید و نمونه بافت کبد برای بررسیهای هیستوپاتولوژی تهیه شد. برای القاء دیابت mg/kg60 استرپتوزوتوسین (STZ) بهصورت داخل صفاقی تزریق شد و پس از مشاهده علائم دیابت (قند خون بالای mg/dL25۰ و پرنوشی) تیمار توسط عصاره و دارو انجام گردید. در موشهای دیابتی در مقایسه با موشهای کنترل، آلبومین سرم کاهش معنیدار و میزان گلوتامیک پیروویک ترانس آمیناز (GPT) و گاما گلوتامیل ترانسفراز (GGT) سرم افزایش معنیدار نشان دادند (0.05>P). تیمار موشهای دیابتی با دوزهای مختلف عصاره سرخهولیک سبب افزایش معنیدار آلبومین سرم و کاهش معنیدار GGT سرم شد (0.05>P). در موشهای دیابتی نکروز هپاتوسیتها، تخریب کامل لبولها و طنابهای رماک و پرخونی نسبتاً شدید مشاهده شد و تیمار با عصاره سرخهولیک سبب پیشگیری از تغییرات پاتولوژیک گردید. نتایج حاصل از این مطالعه نشان داد عصاره سرخهولیک میتواند سبب پیشگیری از اختلال عملکرد و آسیب بافتی کبد در موشهای صحرایی دیابتی گردد. | ||
| کلیدواژهها | ||
| سرخهولیک (Crataegus monogyna Jacq.)؛ دیابت؛ کبد؛ موش صحرایی | ||
| عنوان مقاله [English] | ||
| Effects of hydroalcoholic extract of Crataegus monogyna Jacq. on histopathology and biomarkers of liver injury in diabetic male rats | ||
| نویسندگان [English] | ||
| M. Ahmadinasab1؛ S. Valipour Chahardah Cheric2؛ M. Setorki3 | ||
| 1M.Sc., Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran | ||
| 2Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran | ||
| 3Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran | ||
| چکیده [English] | ||
| Diabetes mellitus is associated with physical, physiological, and pathological changes in the liver tissue. The aim of this study was to investigate the protective effects of hydroalcoholic extract of Crataegus monogyna Jacq. on histopathology and liver function in streptozotocin (STZ)-induced diabetic rats. In this experimental study, Wistar male rats were randomly divided into six groups of eight including control, diabetic, diabetic treated with 100, 200, and 400 (mg/kg) doses of C. monogyna extract and positive control (diabetic treated with captopril). Diabetes was induced by intra-peritoneal injection of a single-dose of STZ (60 mg/kg). After observation of the diabetes symptoms (blood glucose level above 250 mg/dL and polydipsia), treatments were done using extract and drug. After three weeks of treatment with extract and drug intra-peritoneally, the serum level of liver enzymes and albumin was determined and liver tissue samples were prepared for histopathologic examination. In diabetic rats, there was a significant decrease in serum albumin and a significant increase in serum glutamic-pyruvic transaminase (GPT) and gamma glutamyltransferase (GGT) in comparison with healthy rats (P<0.05). Treatment of diabetic rats with different doses of C. monogyna extract increased albumin and decreased GGT in serum significantly (P<0.05). In diabetic rats, hepatocytes necrosis, complete destruction of the liver lobules and Remak bundles and hyperemia were observed, and the treatment with C. monogyna extract prevented the pathologic changes. The results of this study showed that C. monogyna extract can prevent liver tissue degradation and its dysfunction in diabetic rats. | ||
| کلیدواژهها [English] | ||
| Crataegus monogyna Jacq, diabetes, liver, rat | ||
| مراجع | ||
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