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اثر محرک‌های اسید سالیسیلیک، متیل‌جاسمونات و NaCl بر ترکیب‌های بیوشیمیایی کالوس استویا (Stevia rebaudiana Bertoni) در محیط‌کشت‌های جامد و مایع | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 4، دوره 35، شماره 5 - شماره پیاپی 97، آذر و دی 1398، صفحه 731-744 اصل مقاله (653.6 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.123589.2407 | ||
| نویسندگان | ||
| مارال سلملیان1؛ عظیم قاسم نژاد* 2؛ کامبیز مشایخی2 | ||
| 1دانشجوی کارشناسی ارشد، رشته گیاهان دارویی ادویه‌ای و نوشابه‌ای، گروه علوم باغبانی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 2دانشیار، گروه باغبانی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1398.35.731.97.5.1575.1593 استفاده از محرک‌ها روشی کارآمد برای افزایش تولید متابولیت‌های ثانویه گیاهان دارویی در شرایط برون و درون شیشه‌ای به‌شمار می‌رود. این تحقیق به‌منظور بررسی اثر شوری صفر و 50 میلی‌مولار، سالیسیلیک اسید و متیل‌جاسمونات با غلظت‌های صفر و 100 میکرومولار در دو محیط کشت جامد و مایع بر صفات بیوشیمیایی و فعالیت آنزیم فنیل‌آلانین آمونیالیاز (PAL) کالوس گیاه استویا (Stevia rebaudiana Bertoni) در شرایط درون شیشه‌ای انجام شد. این تحقیق با آرایش فاکتوریل دو عاملی بر پایه کاملاً تصادفی در 4 تکرار انجام گردید. محرک‌های مورد استفاده، محیط کشت و اثر متقابل آنها تأثیر معنی‌داری بر صفات اندازه‌گیری شده داشتند. بیشترین میزان تولید فنل و فلاونوئید، در تیمار اسید سالیسیلیک 100 + شوری 50 و تیمار شوری 50 میلی‌مولار در محیط کشت جامد دیده شد. تیمارهای شوری 50 میلی‌مولار، اسید سالیسیلیک 100+ شوری 50 و متیل‌جاسمونات 100+ شوری 50 در محیط کشت جامد سبب افزایش فعالیت آنزیم PAL نسبت به سایر تیمارها شدند. بین آنزیم فنیل‌آلانین آمونیالیاز، فنل و فلاونوئید همبستگی مثبت دیده شد. تیمار شوری 50 میلی‌مولار همچنین سبب افزایش اسید آمینه پرولین شد. بنابراین برای بهبود تولید ترکیب‌های ثانویه‌ای مانند فنل، فلاونوئید و همچنین فعالیت آنزیم فنیل‌آلانین آمونیالیاز در استویا، استفاده از تیمار ترکیبی سالیسیلیک‌اسید (100 میکرومولار) و شوری (50 میکرومولار) در شرایط کشت جامد توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| آنزیم فنیل‌آلانین آمونیالیاز؛ پرولین؛ کشت بافت؛ فنل کل؛ فلاونوئید کل؛ محیط کشت مایع | ||
| عنوان مقاله [English] | ||
| The effect of salicylic acid, methyl jasmonate and NaCl stimulants on biochemical compounds of stevia’s callus in solid and liquid media | ||
| نویسندگان [English] | ||
| M. Salmalian1؛ A. Ghasemnejad2؛ K. Mashayekhi2 | ||
| 1M.Sc. student in Medicinal and Aromatic Plants, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| Using stimulants is an efficient way to increase the production of secondary metabolites of medicinal plants under in vivo and in vitro conditions. The present study aimed to investigate the effect of NaCl (0 and 50 mM), salicylic acid, and methyl jasmonate (each in 0 and 100 μM) in solid and liquid culture media on the biochemical properties and phenylalanine ammonia-lyase (PAL) activity of stevia callus under in vitro conditions. This study was conducted in a completely randomized design with a two-factor factorial arrangement in four replications. The stimuli used, culture medium and their interactions had a significant effect on the measured traits. The highest amount of phenol and flavonoid production was observed in salicylic acid 100 + salinity 50 and salinity 50 treatments in solid culture medium. Treatments including salinity 50, salicylic acid 100 + salinity 50 and methyl jasmonate 100 + salinity 50 in the solid medium increased PAL enzyme activity compared to other treatments. PAL enzyme, phenol and flavonoid were positively correlated each other. The 50 mM salinity treatment also increased proline amino acid content. Therefore, in order to improve the production of secondary compounds such as phenol, flavonoids and also PAL enzyme activity in stevia, a combination of salicylic acid (100 μM) and salinity (50 mM) is recommended under solid culture medium conditions. | ||
| کلیدواژه‌ها [English] | ||
| Phenylalanine ammonialyase, proline, tissue culture, phenol, flavonoid, liquid media | ||
| مراجع | ||
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