| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 3,005 |
| تعداد مقالات | 33,631 |
| تعداد مشاهده مقاله | 44,479,804 |
| تعداد دریافت فایل اصل مقاله | 30,137,077 |
ارزیابی بیمارگری Steinernema feltiae روی بید گوجهفرنگی، Tuta absoluta در شرایط آزمایشگاه و گلخانه | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 3، دوره 6، شماره 2 - شماره پیاپی 12، اسفند 1397، صفحه 31-41 اصل مقاله (151.27 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2019.119557 | ||
| نویسندگان | ||
| المیرا ابوترابی* ؛ شهرام فرخی | ||
| مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران | ||
| چکیده | ||
| بید گوجهفرنگی (Tuta absoluta)، یکی از آفات مخرّب و کلیدی گوجهفرنگی است که در صورت طغیان محصول را به‌طور کامل از بین می‌برد. در این بررسی بیمارگری نماتدSteinernema feltiae Filipjev روی سنین مختلف لاروی T. absolutaدر شرایط آزمایشگاه و همچنین غلظت مؤثر نماتد در کنترل آفت در شرایط گلخانه ارزیابی شد. در مطالعه آزمایشگاهی، نتایج تاثیر غلظتهای صفر، 125، 200، 355، 632 و 1124 عدد لارو عفونتزای سن سوم نماتد (Infective juveniles, IJs) روی لاروهای سنین دو تا چهار بید گوجهفرنگی نشان داد که 48 ساعت پس از آلوده‌سازی، نماتد قادر به بیمارکردن سنین لاروی آفت می‌باشد و با افزایش تراکم جمعیت نماتد، درصد تلفات نیز افزایش می‌یابد. درصد تلفات سنین لاروی T. absoluta، در سطح احتمال 1% اختلاف معنیداری با یکدیگر داشتند. درصد مرگ و میر لاروهای سنین دوم، سوم و چهارم در بالاترین غلظت نماتد 1124 عدد IJs به ترتیب 5/31، 6/75 و 9/85 درصد بدست آمد. این مقادیر در کمترین غلظت نماتد به ترتیب معادل 8/16 ، 7/26 و 3/30 درصد بود. برای انجام آزمایش در شرایط گلخانه، سه تیمار شامل مؤثرترین غلظت نماتد معادل 1124 عدد IJs در یک میلی‌لیتر آب، فراورده بِلتیرول باکتریBacillus thuringiensis (Bt)،با غلظت 5/2 در هزار (32% WP) به‌عنوان تیمار مقایسه و تیمار شاهد بدون نماتد در شش تکرار در نظر گرفته شد. میانگین تلفات سنین مختلف لاروی بید گوجهفرنگی در تیمار S. feltiae با 9/2±5/77 درصد در مقایسه با نتایج حاصل از تاثیر فراوردهBt با 7/13±40 درصد و تیمار شاهد، اختلاف معنیداری داشت. نتایج به دست آمده حاکی از پتانسیل نماتد S. feltiae در بیمارگری لاروهای بید گوجهفرنگی و تأثیر معنی‌دار آن در ایجاد تلفات روی این آفت می‌باشد. | ||
| کلیدواژه‌ها | ||
| بید گوجهفرنگی؛ کنترل بیولوژیک؛ نماتد بیمارگر حشرات | ||
| عنوان مقاله [English] | ||
| Efficacy of a native isolate of Steinernema feltiae on tomato leafminer (Tuta absoluta) under laboratory and greenhouse conditions | ||
| نویسندگان [English] | ||
| Shahram Farrokhi؛ Shahram Farrokhi | ||
| Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| The tomato leafminer, Tuta absoluta (Meyrick) (Lep., Gelechiidae) is a devastating key pest of tomato, and its damage may reach up to 100%. Here, the potential of the infective juveniles (IJs) of the entomopathogenic nematode Steinernema feltiae Filipjev species on different larval stadia of T. absoluta was studied under laboratory conditions and the ability of most effective nematode concentration when applied in the greenhouse conditions. In the in vitro, the six concentrations of third-infective juveniles S. feltiae (0, 125, 200, 355, 632 and 1124 IJs/ml) were tested against the three larval instars, L2, L3 and L4 of T. absoluta. Forty eight hours after infection, the entomopathogenic nematode was able to infect all instars of the pest. With increasing density of S. feltiae, the pest larva mortality also increased with a significant difference at 1% probability level. The percentage mortality of L2, L3 and L4 at the highest concentration of nematodes (1124 IJs) were 31.5, 75.6 and 85.9%, respectively. These values at the lowest concentration of nematodes (125 IJs) were 16.8, 26.7, and 30.3%. In the greenhouse test, three treatments included; S. feltiae (1124 IJs/ml), Bacillus thuringiensis (0.25%, Belthirul 32% WP, Probelte Co.) and control in a completely randomized design with six replicates were conducted. The percentage mortality of different T. absoluta larval stages by S. feltiae and B. thuringiensis treatments were 77.5%±2.9 and 40%±13.7 with a significant difference at 1% probability level. The results indicate the potential of S. feltiae as a native entomopathogenic nematode could be used in an integrated management measure in controlling of the T. absoluta. | ||
| کلیدواژه‌ها [English] | ||
| biological control, tomato leaf miner, Steinernema feltiae | ||
| مراجع | ||
|
Abbott, W.S.1925. A method of computing effectiveness of an insecticide. Journal of Economical Entomology, 18: 265–267. Abootorabi, E. 2011. The control feasibility of Ectomyelois ceratoniae by Entomopathogenic nematode. National Pomegranate Symposium, Ferdowsi- Iran; 28–29. Abootorabi, E. & Valizadeh, A. 2012. Preliminary study of using nematode parasites of insects (Nematodes: Entomopathogenic) to control of maize borer (Ostrinia nubilalis). The first National Congress of Monitoring and Forecasting in Plant Protection, Borujerd; Iran; 14–15. Abootorabi, E. 2014. Report of native isolate pathogenicity of Steinernema feltiae on tomato leafminer, Tuta absoluta. Biocontrol in Plant Protection, 1: 107–109. Arthurs, S., Heinz, K.M. & Prasifka, J.R. 2004. An analysis of using entomopathogenic nematodes against above ground pest. Bulletin of Entomological Research, 94: 297–306. El- Arnaouty, S. A., Pizzol, J., Galal, H. H., Kortam, M. N., Afifi, A. L., Beyssat, V., Desneux, N., Biondi, A & Heikal, I. H. 2014. Assessment of two Trichogramma species for control of Tuta absoluta in North African Tomato Greenhouses. African Entomology, 22: 801–809. Baniameri, V. & Cheraghian, A. 2011. The current status of Tuta absoluta in Iran and initial control strategies In: International symposium on management of Tuta absoluta (tomato borer) proceeding. Agadir, Morocco, November 16-18, 2011. Batalla- Carrera, L., Morton, A. & Garcia- delpino, F. 2010. Efficacy of entomopathogenic nematodes against the tomato leafminer Tuta absoluta in laboratory and greenhouse conditions. BiolControl, 55: 523–530. Berbert-Molina, M.A., Prata, A. M.R., Pessanha, L.G. & Silveira, M.M. 2008. Kinetics of Bacillus thuringiensis var. israelensis growth on high glucose concentrations. Journal of Industrial Microbiology and Biotechnology, 35(11): 1397–1404. Bloem, S. & Esther, S. 2011. New pest response guidelines tomato leafminer (Tuta absoluta). USDA. Chailleux, A., Bearez, P., Pizzol, J., Amiens-Desneux, E., Ramirez-Romero, R. & Desneux, N.2013. Potential for combined use of parasitoids and generalist predators for biological control of the key invasive tomato pest Tuta absoluta. Journal of Pest Science, 86(3): 533–541. De Backer, L., Haubruge, E., Caparros Megido, R & Verheggen, F. 2014. Macrolophus pygmaeus as an efficient predator of the tomato leafminer Tuta absoluta in Europe. Biotechnology, Agronomy, Society and Environment 18: 536–543. Desneux, N., Wajnberg, E., Wyckhuys., K.A.G., Burgio, G., Arpaia, S., Narvaez-Vasquez, C.A., Gonzalez-Cabrera, J., Ruescas, D.C., Tabone, E., Frandon, J., Pizzol, J., Poncet, C., Cabello, T. & Urbaneja, A. 2010. Biological invasion of European tomato crops by Tuta absoluta: ecology, geographic expansion and prospects for biological control. Journal of Pest Science, 83: 197–215. Desneux, N., Luna, M.G., Guillemaud, T. & Urbaneja, A. 2011. The invasive South American tomato pinworm, Tuta absoluta, continues to spread in Afro- Eurasia and beyond: the new threat to tomato world production. Journal of Pest Science, 84: 403–408. Dowds, B.C.A & Peters, A. 2002. Virulence mechanisms. Pp. 79-98 In R. Gaugler, ed. Entomopathogenic nematology. New York, NY: CABI. Eivazian Kary, N., Niknam, G., Griffin, C.T & Mohammadi, S.A. 2009. A survey of entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae (Nematoda: Rhabditida) in the north-west of Iran. Nematology, 11 (1): 107-116. Garcia-del-Pino, F., Alabern, X. & Morton, A. 2013. Efficacy of soil treatments of entomopathogenic nematodes against the larvae, pupa and adults of Tuta absoluta and their intraction with the insecticides used against this insect. BioControl, 58: 723–731. Giustolin, T.A., Venderamim, J.D., Alves, S.B., Vieira, S.A. & Pereira, R.M. 2001. Susceptibility of Tuta absoluta (Meyrick) (Lepidoptra: Gelechiidae) reared on two species of Lycopersicon to Bacillus thuringiensis var Kurstaki. Journal of Applied Entomology, 125: 551–556. Gonzalez- Cabrera, J., Molla, O., Moton, H & Urbaneja, A. 2011. Efficacy of Bacillus thuringiensis (Berliner) for controlling the tomato borer, of Tuta absoluta (Meyrick) (Lepidoptra: Gelechiidae). BioControl, 56: 71–80. Hallem, E.A., Dillman, A.R., Hong, A.V., Zhang, Y., Yano, J.M., DeMarco, S.F. & Sternberg, P. W. 2011. A sensory code for host seeking in parasitic nematodes. Current Biology, 21: 377–383. Heierson, A., Sidén, I., Kivaisi, A & Boman, H.G.(1986). Bacteriophage-resistant mutants of Bacillus thuringiensis with decreased virulence in pupae of Hyalophora cecropia. Journal of Bacteriology, 167(1): 18–24. Hunt, D.J. 2007. Entomopathogenic Nematodes: Systematics, Phylogeny of Bacterial Symbionts, Nematology Monographs and Perspectives. Koninklijke Brill NV, Leiden. Isman, M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review Entomology, 51: 45–66. Kamali, S., Karimi, J., Hosseini, M., Campos-Herrera, R & Duncan, L. W. 2013. Biocontrol potential of the entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae on cucurbit fly, Dacus ciliates (Diptera: Tephritidae). Biocontrol Science and Technology, 23(11): 1307–1323. Karimi, J., Kharazi-Pakdel, A., Yoshiga, T., Koohi Habibi, M & Hassani- Kakhki, M. 2011. Characterization of Xenorhabdus (Y-Proteobacteria) strains associated bacteria with the Steinernema (Nematode: Steinernematidae) isolates from Iran. Journal of Entomological Society of Iran, 31 (1): 57–69. Kaya, H.K. & Stock, S.P. 1997. Techniques in insect nematology. In: Lacey, L. A. (ed.), Manual of techniques in insect pathology. Academic press, London, UK. Koppenhofer, A.M. & Grewal, P.S. 2005. Compatibility and Interactions with Agrochemicals and Other Biocontrol Agents. In: Grewal, P., Ehlers, R. & Shapiro-llan, D. (eds.) Nematodes as biocontrol agents. CABI International. Mollá, O., González-Cabrera, J. & Urbaneja, A. 2011. The combined use of Bacillus thuringiensis and Nesidiocoris tenuis against the tomato borer Tuta absoluta. BioControl, 56: 883–891. Nikdel, M., Niknam, G., Griffin, C.T. & Eivazian Kary, N. 2010. Diversity of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) from Arasbaran forests and rangelands in north-west Iran. Nematology, 12: 767–773. Petras, S.F. & Casida, L.E. 1985. Survival of Bacillus thuringiensis spores in soil. Applied and Environmental Microbiology, 50(6): 1496–1501. Reyes, M., Rocha, K., Alarco ´n, L., Siegwart, M & Sauphanor, B. 2012. Metabolic mechanisms involved in the resistance of field populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to spinosad. Pesticide Biochemistry and Physiology, 102:45–50. Sharma, M.P., Sharma, A.N. & Hussaini, S.S. 2011. Entomopathogenic nematodes, a potential microbial biopesticide: Mass production and commercialization status- a mini review. Archives of Phytopathology and Plant Production, 44: 855–870. Stock, S.P. & Goodrich- Blair, H. 2012. Nematode parasites, pathogens and associates of insects and invertebrates of economic importance. Pp. 373- 426. In: Lacey, L. A. (ed.), Manual of Techniques in Insect Pathology. 2nd ed. Academic Press, San Diego. Tanha Maafi, Z., Ebrahimi, N., Abootorabi, E & Spiridonov, S. E. 2006. Record of two Steinernematid species from Iran. 17th Iranian Plant Protection Congress, 482p. Urbaneja, A., Desneux, N., Gabarra, R., Arno, J., Gonzalez-Cabrera, J., Mafra Neto, A., Stoltman, L., Pinto, A. de S & Parra, J.R.P. 2013. Biology, ecology and management of the South American tomato pinworm, Tuta absoluta. Pp. 98-125. In: Pena, J.E. (ed), Potential Invasive Pests of Agricultural Crops. CAB International, Wallingford. Van Damme, V.M., KEG Beck, B., Berckmoes, E., Moerkens, R., Wittemans, L., De Vis, R., Nuyttens, D., Casteels, H.F., Maes, M., Tirry, L & De Clercq, P. 2015. Efficacy of entomopathogenic nematodes against larvae of Tuta absoluta in the Laboratory. Pest manag Society of Chemical Industry (SCI). 8 P. | ||
|
آمار تعداد مشاهده مقاله: 438 تعداد دریافت فایل اصل مقاله: 522 |
||