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بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی با استفاده از پیش‌تیمار نیترو پروساید سدیم تحت تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 6، دوره 8، شماره 1 - شماره پیاپی 15، تیر 1398، صفحه 67-81 اصل مقاله (1.1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.114505.1108 | ||
| نویسندگان | ||
| حسین رضا روحی* 1؛ علی مرادی2؛ مریم ثمن3؛ یاسین محمدی4؛ علی رضا شاهبداغلو5 | ||
| 1دانشگاه بوعلی سینا | ||
| 2استادیار گروه زراعت و اصلاح نباتات دانشگاه یاسوج | ||
| 3دانشگاه پیام نور | ||
| 4دانشجوی کارشناسی ارشد سابق علوم و تکنولوژی بذر | ||
| 5کارشناس پژوهشی | ||
| چکیده | ||
| به‌منظور بررسی اثر نیترو پروساید سدیم به عنوان ترکیب آزاد کتتده اکسید نیتروژن در بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر دانشکده کشاورزی دانشگاه بوعلی سینا همدان انجام شد. پیش‌تیمار غلظت‌های مختلف نیتروپروساید سدیم شامل صفر (پیش‌تیمار شده با آب)، 25، 50 و 75 میکرومولار در سطوح خشکی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال مورد ارزیابی قرار گرفت. شاخص‌های درصد جوانه‌زنی، سرعت جوانه‌زنی، بنیه بذر، طول گیاهچه، هیدرات‌های کربن محلول، پروتئین‌های محلول، هدایت الکترولیتی غشاء، مالون دی‌آلدهید، فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز بررسی شد. نتایج نشان داد پیش‌تیمار بذرها با غلظت‌های مختلف نیترو پروساید سدیم، از کاهش معنی‌دار شاخص‌های جوانه‌زنی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی جلوگیری نمود. به‌طوری‌که در پتانسیل 6/0- مگاپاسکال، پیش‌تیمار بذرها با 75 میکرومولار نیترو پروساید سدیم سبب افزایش سرعت جوانه‌زنی، شاخص بنیه بذر، قندها و پروتئین‌های محلول به-ترتیب به‌میزان 25، 1/214، 8/90، 1/117 درصد و برای فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز به‌ترتیب حدود 34، 5/14و 4/41 درصد نسبت به شاهد گردید. بنابراین پیش‌تیمار نیتروپروساید سدیم به‌ویژه با غلظت 75 میکرومولار به دلیل کاهش تنش اکسیداتیو ناشی از زوال و خشکی برای کدوی پوست کاغذی قابل توصیه است. | ||
| کلیدواژه‌ها | ||
| پیش‌تیمار؛ جوانه‌زنی؛ زوال بذر؛ کدوی پوست کاغذی؛ نیترو پروساید سدیم | ||
| عنوان مقاله [English] | ||
| Seed priming with SNP improves the performance of aged pumpkin (Cucurbita pepo L.) seeds under drought stress | ||
| نویسندگان [English] | ||
| Hossein Reza Rouhi1؛ َAli Moradi2؛ Maryam Saman3؛ Yasin Mohammadi4؛ Alireza Shahbodaghlo5 | ||
| 1گروه زراعت و اصلاح نباتات | ||
| 2Associate Professor, Department of Agronomy and Plant breeding, Faculty of Agriculture, University of Yasouj, Iran | ||
| 3گروه علوم کشاورزی، تهران | ||
| 4دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، | ||
| 5گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| چکیده [English] | ||
| In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations of sodium nitroprusside including 0 (hydropriming), 25, 50 and 75 µM in drought stress levels (0, -0.2, -0.46 and -0.6 MPa) was performed. Germination percentage, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of sodium nitroprusside mitigated significant decrease of germination parameters of aged seeds under drought stress. At -0.6 MPa, seed priming with 75 µM of sodium nitroprusside increased germination rate, vigor index , soluble carbohydrates and proteins 25, 214.1, 90.8, 117.1%, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 34, 14.5, 41.4% in comparison to control, respectively. Therefore, seed priming with sodium nitroprusside specialy with 75 µM is suggested for aged pumpkin seeds by decreasing oxidative stress due to drought stress. | ||
| کلیدواژه‌ها [English] | ||
| germination, pumpkin, seed deterioration, seed priming, sodium nitroprusside | ||
| مراجع | ||
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