| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,530,806 |
| تعداد دریافت فایل اصل مقاله | 90,479,591 |
اثربخشی کاربرد همزمان ریزوبیوم بومی و میکوریز بر پوسیدگی ریشه و عملکرد لوبیا قرمز (.Phaseolus vulgaris L) در استان لرستان | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 4، دوره 6، شماره 1 - شماره پیاپی 11، شهریور 1397، صفحه 43-58 اصل مقاله (186.11 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2018.119375 | ||
| نویسندگان | ||
| علی دهقانی* 1؛ ناصر پنجهکه2؛ هادی اسدی رحمانی3؛ محمد سالاری2؛ مصطفی درویشنیا4 | ||
| 1مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، خرمآباد، ایران | ||
| 2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| 3مؤسسه تحقیقات خاک و آب کشور، سازمان تحقیقات،آموزش و ترویج کشاورزی، کرج، ایران | ||
| 4گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران | ||
| چکیده | ||
| پوسیدگی ریشه لوبیا ناشی از قارچ Fusarium solani f.sp. phaseoli، بیماری مهم و اقتصادی محصول لوبیا در ایران است. امروزه بهرهگیری از روشهای منطبق با کشاورزی پایدار و حفظ محیط زیست در مدیریت بیمارگرهای گیاهی و نیز تا حدّ امکان به حداقل رساندن کاربرد سموم شیمیایی، ضرورت بسیاری دارد. این آزمایش جهت بررسی اثر کاربرد تیمارهای انفرادی و ترکیبی ریزوبیوم بومی، قارچهای میکوریز و مقایسه با تیمار شیمیایی در مزرعه در قالب طرح بلوک کامل تصادفی با سه تکرار در سال زراعی 1396 در مزرعه آزمایشی ایستگاه تحقیقات سراب چنگایی با استفاده از لوبیا قرمز رقم افق اجرا شد. تیمارهای آزمایش شامل جدایه برتر ریزوبیوم بومی Rhizobium etli b.v. phaseoli با شناسه ملیCCSM_B011401 (R)، میکوریز Rhizophagus irregularis (M1)، میکوریزهای R. irregularis+ Funneliformis mosseae (M1+M2)، میکوریزهای R. irregularis+ F. mosseae بهعلاوه باکتری ریزوبیوم R. etli b.v. phaseoli(M1+M2+R)، باکتری ریزوبیوم R. etli b.v. phaseoli + میکوریز R. irregularis (R+M1)، تیمار شیمیایی کاربرد قارچکش (F) و کرتهای شاهد که دارای آلودگی به بیمارگر، ولی بدون تلقیح ریزوبیوم و قارچهای میکوریزی بود. ریزوبیوم به شکل پودری با آغشته کردن بذور و قارچهای میکوریزی با افزودن به بستر بذر در زمان کاشت مایهزنی شد. نتایج نشان داد که بهترین تیمار آزمایش، تیمار ترکیبی تلقیح همزمان ریزوبیوم بومی و قارچهای میکوریزی بود که تعداد دانه در غلاف، وزن صد دانه، عملکرد دانه و عملکرد بیولوژیک را به طور محسوس و معنی داری به ترتیب تا 34، 9/24 ، 6/51 و 38 درصد نسبت به شاهد افزایش و وقوع بیماری پوسیدگی ریشه لوبیا را به میزان 89 درصد کاهش داد. یافتههای این پژوهش نشان میدهد که همافزایی ناشی از تلقیح همزمان عوامل بیولوژیک ریزوبیوم بومی برتر و قارچهای میکوریزی میتواند به دلیل کاهش چشمگیر وقوع بیماری و بهبود عملکرد و اجزاء عملکرد لوبیا جایگزین مناسبی برای سموم و کودهای شیمیایی در این محصول باشد. | ||
| کلیدواژهها | ||
| تلقیح همزمان؛ هم افزایی؛ پوسیدگی ریشه؛ مهار زیستی؛ کنترل شیمیایی؛ کود زیستی | ||
| عنوان مقاله [English] | ||
| Effectiveness of simultaneous application of indigenous rhizobium and arbuscular mycorrhiza on root rot disease and yield of red bean (Phaseolus vulgaris L.) in Lorestan Province | ||
| نویسندگان [English] | ||
| Ali Dehghani1؛ Naser Panjehkeh2؛ Hadi Asadi Rahmani3؛ Mohammad Salari2؛ Mostafa Darvishnia4 | ||
| 1Lorestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extention Organization (AREEO), Khorramabad, Iran | ||
| 2Plant Protection Department, Faculty of Agriculture, University of Zabol | ||
| 3Soil Biology Research Department, National Iranian Soil and Water Research Institute, Research, Education and Extention Organization (AREEO), Karaj, Iran | ||
| 4Plant Protection Department, Faculty of Agriculture, Lorestan University, Khoramabad, Iran | ||
| چکیده [English] | ||
| Fusarium root rot of common beans is an important economic disease in Iran. It is broadly suggested that farmers use ecofriendly methods of plant disease management, compatible with the sustainable agriculture to minimize the application of pesticides in the fields. The current study was conducted to investigate the effectiveness of native rhizobium, mycorrhiza and chemical treatment. The experiment was carried out in a randomized complete block design in a field experiment at Sarab Changaie Research Station in 2017 with Ofogh red-bean cultivar. The trial treatments exerted as follow, superior native rhizobium isolate Rhizobium etli b.v. phaseoli (CCSM_B011401)(R), Rhizophagus irregularis(M2), Rhizophagus irregularis + Funneliformis mosseae (M1+M2), R. irregularis + F. mosseae + R. etli b.v. phaseoli (R+M1+M2), R. irregularis + R. etli b.v. phaseoli (R+M2), fungicide treatment and control.Rhizobium powder was applied as seed treatment and mycorrhiza was added directly in seedbed during the sowing of seeds. The best result was achieved from co-inoculation of arbuscular mycorrhiza fungi and indigenous rhizobium, which showed a significant increase of the number of seeds per pod, 100 seeds weight, grain yield and biomass yield as 34, 24.9, 51.6 and 38 percent compared to control. In addition, the co-inoculation treatment reduced the incidence of bean root rot disease up to 89%. Results indicated that the synergism of indigenous rhizobia and arbuscular mycorrhiza could be useful for managing of common bean root rot disease, reducing the use of pesticides and producing the crop in a sustainable and optimized manner. | ||
| کلیدواژهها [English] | ||
| co-inoculation, synergism, root rot, biological control, chemical control, biofertilizer | ||
| مراجع | ||
|
Ahmed, M. & Khan, M.S. 2010. Influence of selective herbicides on plant growth promoting traits of phosphate solubilizing Entrobacter asburiae Strain PS2. Research Journal of Microbiology, 5(9): 849–857. Ahmadi, K., Gholizadeh, H., Ebadzadeh, H., Hoseinpour, R., Addeshah, H., Kazemian, A. and Rafiee, M. 2016. Agriculture statistics. Ministry of Jihad e Agriculture of Iran. Tehran, Iran.Vol:1, 125p. Amanifar, N., Ghadirian, M. And Salehi, F. 2017. Common bean cultivars and genotypes reaction to Fusarium solani f.sp. phaseolina with and without wet stress in greenhouse condition. Plant Pests and Diseases, 85(1):56–68. Arfaoui, A, El Hadrami, A., Mabrouk, Y., Sifi, B., Boudabous, A., El Hadrami, I., Daayf, F., Che´rif, M. 2007. Treatment of chickpea with Rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by Fusarium oxysporum f.sp. ciceris. Plant Physiology and Biochemistry, 45:470–479. Ardakani, M.R., Maleki, S., Aghayri, F., Rejali, F. & Faregh, A.H. 2014. Tripartit symbiosis of lentil (Lens culinaris L.) Mycorrhiza and Azospirillium brosilense under rainfed condition. In: proceeding of the 4th ISOFAR scientific conference Building Organic Bridges, Turkey, 691–693. Avis T.J., Gravel, V., Antoun, H. & Tweddell, R.J. 2008. Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil Biology and Biochemistry, 40: 1733–1740. Ardakani, M.R., Pietsch, G., Wanek, W., Shweiger, P., Moghaddam, A. & Friedel, J.K. 2009. Nitrogen fixation and yield of lucerne (Medicago sativa L.), as affected by co-inoculation with Synorrhizobium meliloti and arbuscular mycorrhiza under dry organic farming condition. American-Eurasian Journal of Agricultural and Environmental Science, 6(2): 173–183. Babalola, O. O. & Ekindolire, A. M. 2012. Identification of native rhizobacteria peculiar to selected food crops in Mmabatho municipality of South Africa. Avalable in: http://www.tandfonline.com/loi/tbah20. Bhale, U.N., Bansode, S.A. and Simranjeet, S. 2018. Multifactorial Role of Arbuscular Mycorrhizae in Agroecosystem. Avialable in: https://www.researchgate.net/publication/327720150 Blair, M.W., Astudillo, C., Grusak, M.A., Graham, R. and Beebe, S.E. 2009. Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.). Molecular Breeding, 23: 197–207. Abd-Alla, M.H., Elsadek El-Enany, Nafadi, N.A., Khalaf, D.M. and Morsym F.M. 2014. Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkalin soil. Microbiological Research, 169: 49–58. Dehghani, A., Panjehkah, N., Darvishnia, M., Salari, M. and Asadi Rahmani, H. 2019. Importance and climatic distribution of pathogenic fungi associated with bean root and crown in Lorestan Province. Plant Pests and Diseases (In press) Dhingra, O.D. and Sinclair, J.B. 1995. Basic Plant Pathology Methods. CRC Lewis Publishers. 433p. Dutta, S., Mishra, A.K. and Dileep-Kumar, B.S. 2008. Induction of systemic resistance against fusarial wilt in Pigeon pea through interaction of plant growth promoting rhizobacteria and rhizobia. Soil Biology and Biochemistry. 40:452–461. Egamberdieva, D., Wirth, S., Li, L., Abd-Allah, E. F. and Lindstrom, K. 2017. Microbial cooperation in the rhizosphere improves liquorice growth under salt stress. Avalable in: http://www.tandfonline.com/loi/kbie20. Elkoca E., Kantar F. and fiahin F: Influence of Nitrogen Fixing and Phosphorus Solubilizing Bacteria on the Nodulation, Plant Growth, and Yield of Chickpea. Journal of Plant Nutrition. 2008: 31: 157–171. Estevez De Jensen, C., Kurle, J.E. and Percich, J.A. 2004. Integrated management of edaphic and biotic factors limiting yield of irrigated soybean and dry bean in Minnesota. Field Crop Research. 86: 211–224. Farfour, S. and Al-Saman, M.A. 2014. Root-rot and stem-canker control in faba bean plants by using some biofrtilizers agents. Plant Pathology and Microbiology, 5: 1–6. Food & Agriculture Organization (FAO). 2015. FAO statistics. Available in: http//www.fao.org Ghasemzadeh-Ganjehei, M. & Asgharzadeh, A. 2013. Effects of Rhizobiums sush inoculation and fertilizer on yield and yield components of chickpea (Cicer arietinum) in Khorasan-Razavi. Iranian Journal of Pulses Research, 4(1): 51–58. Gouveia, C.S., Freitas, G., Debrito, J.H., Slaski, J.J. and de Cavalho, M. A.P. 2014. Nutritional and mineral variability in 52 accessions of common bean varieties (Phaseolus vulgaris L.) from Madeira Island. Agricultural Science, vol 5: 317–329. Hall, R. 1996. Inoculum dynamics of Fusarium solani f.sp. phaseoli and management of Fusarium root rot. In: Hall, R.(Ed.), Principle and Practice of Managing Soilborn Plant Pathogens. The American Phytopathological Society, St. Paul, MN, pp. 279–320. Harvenson, R.M., Smith, J.A. and Stroup, W.W. 2005. Improving root health and yield of dry bean in the Nebraska panhandle with a new technique for reducing soil compaction. Plant Disease, 89: 279–284. Heydari, A., and Pasarakli, M. A. 2010. Review on biological control of fungal plant pathogens using microbial antagonists. Journal of Biological Science, 10:273–290 Hoseini, N. M. 2008. Legume Crop and Yield. Tehran University Publication. 284p. Hu, J.L., Lin, X.G., Wang, J.H., Shen, W.S., Wu, S., Peng, S. P. and Mao, T.T. 2010. Arbuscular mycorrhizal fungal inoculation enhances suppression of cucumber Fusarium wilt in Greenhouse soils. Pedosphere, 20(5): 586–593. Itelima J.U.Bang, W.J., Sila, M.D., Onyimba, I.A. & Egbere, O.J. 2018. Biofertilizer, A key player in enhancing soil fertility and crop productivity. Microbiological Biotechnology Report, Vol 2(1) Kalantari, S. 2011. Biological control of common bean root rot using microbial agent in Zanjan. Masters thesis, Zanjan University, 100p. Kanouni, L., Larous, L. and Mezaache Aichour, S. 2018. Inhibitory effect of rhizobia isolated from several leguminous against phytopathogenic fungi. Annual Research & Review in Biology, 22(6): 1–16. Kaur, C., Maini, P. & Shukla, N.P. 2007. Effect of captan and carbendazim fungicides on nodulation and biological nitrogen fixation in soybean. Asian Journal Experimental Science, vol 21(2): 385- 388. Korir, H., Mungai, N. W., Thuita, M. & Masso, C. 2017. Co-inoculation Effect of Rhizobia and Plant Growth Promoting Rhizobacteria on Common Bean Growth in a Low Phosphorus Soil. Available in: https://www.researchgate.net/publication/313422508 Lak, M.R. 2016. Determination of the main agents of bean root rot and evaluation of potential and damage in Khomein. Final report of project. Markazi Agricultural and Natural Resources Research and Education Center. 20p. Lak, M.R. & Khodashenas, M. 2008. Effect of different sources of nitrogen on bean root rot disease. Final report, Markazi Agricultural and Natural Resources Center. 30p. Lee, J.Y. and Song, S.H. 2007. Evaluation of groundwater quality in coastal areas: implications for sustainable agriculture. Environmental Geology, 52: 1231–1242. Liu, Y., Wu, L., Baddeley J.A. and Watson C.A. 2011. Models of biological nitrogen fixation of legumes. Agronomic Sustainability, 31, 155–172. Linderman, R.G.1994. Role of VAM fugi in biocontrol. In: Mycorrhizae and plant health, eds, F.L. Pfleger & R.G. Linderman. APS Press, St. Paul. MN, pp. 1–26. Macedo, R., Patricia Sales, L., Yoshida, F. Lemes Silva-Abud, L. & Junior, M. L.2017. Potential worldwide distruction of Fusarium dry root rot in common beans based on the optimal environment fir disease occurrence. Available in: http://journals.plos.org/plosone/article?id=10.137/journal.pone.0187770 Maksimov I.V., Abizgil’dina, R.R., Pusenkova, L.I. 2011. Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens. Applied Biochemistry Microbiology, 47:333–345. Maloy, O.C. 2005. Plant Disease Management. The Plant Health Instructor. Avalable in: https://www.apsnet .org/ edcenter/intropp/topics/Documents/PlantDiseaseManagement.aspx Matloob, A.H. & Juber, K.S. 2013. Biological control of bean root rot disease caused by Rhizoctonia solani under greenhouse and field conditions. Agricultural & Biology Journal of North America, 4(5): 512–519. Mazen, M.M., El-Batanony, N.H., Abd El-Monium, M.M. & Massoud, O.N. 2008. Cultural filtrate of Rhizobium spp. and arbuscular mycorrhiza are potential biological control agents against root rot fungal diseases of faba bean. Global Journal of Biotechnology & Biochemistry, 3(1):32–41. Messina, V. 2014. Nutritional and health benefits of dried beans. American Society for Nutrition. American Journal of Clinical Nutrition, 437–442. Muleta, D. 2017. Legume Response to Arbuscular Mycorrhizal Fungi Inoculation in Sustainable Agriculture. Microbes for Legume Improvement, Avalable in: https://link.springer.com/chapter/10.1007/978-3-319-59174-2_10 Naseri, B. 2008. Root rot of common bean in Zanjan, Iran: major pathogens and yield loss estimates. Australian Plant Pathology, 37: 548–551. Naseri, B. 2014. Bean production and fusarium root rot in diverse soil environments in iran. Journal of Soil Science and Plant Nutrition, 14(1): 177–188. Naseri, B. and Mousavi, S.S. 2015. Root rot pathogens in field soil, roots and seeds in relation to common bean (Phaseolus vulgaris L.), disease and seed production. International Journal of Pest Management. Published online:http://www.tandfonline.com Nicoli, A., Zambolim, l., Paula Jonior, T.J., Viera, R.F., Teixeira, H. & Carneiro, J.E.S. 2013. Clamidospore concenteration for assessment of Fusarium root rot on common bean. Tropical Plant Pathology, 38(2): 149–151. Odoh, C. K. 2017. Plant Growth Promoting Rhizobacteria (PGPR): A Bioprotectant bioinoculant for Sustainable Agrobiology. International Journal of Advanced Research in Biological Sciences, Vol. 4(5): 123–141. Pawar, V.A., Pawar, P.R., Bhosale, A.M. & Chavan, V. 2014. Effect of Rhizobium on seed germination and growth of plants. Journal of Academia and Industrial Research, 3(2): 84–88. Rezaee, A. M. 1995. Concepts of probability and statistics. Mashhad publishing co. 431p. Sabbaghpour, S. H. 2014. National document of iranian research strategy. Publication of Agricultureal Research, Education and Extention Organization (AREEO), 417p. Safapour, M., Ardakani, M.R., Khaghani, Sh., Rejali, F., Zargari, K., Changizi, M. and Teimuri, M.2011. Response of yield and yield components of three red bean (Phaseolus vulgaris L.) genotyps to co-inoculation with Glomus intraradices and Rhizobium phaseoli. American-Eurasian Journal of Agriculture & Environmental Sciences, 11(3): 398–405. Safarloo, Z. and Hemmati, R. 2014. Identification and pathogenesis study of Fusarium species related to commonbean root rot in Zanjan Province. Aplied Research in Plant Protection, 3(1): 77–92. Saremi, H. and Saremi, H. 2014. Isolation of the most common Fusarium species and the effect of soil solarization on main pathogenic species in different climatic zones of Iran. Europian Journal Plant Pathology, 137: 585–596. Schwartz, H.F., Steadman, J. R., Hall, R. and Forster, R.L. 2005. Compendium of Bean Diseases. APS Press, St. Paul, Minnesota, USA. 109 p. Shekari, F., Esmaeelpour, B. and Shekari, F. 2010. The physiology of vegetable crops. Vol 2. Danesh Publication, Zanjan. 336p. Shepherd M., Nguyen L., Jones ME., Nichols J.D. and Carpenter F.L. 2007. A method for assessing arbuscular mycorrhizal fungi group distribution in tree roots by intergenic transcribed sequence variation. Plant Soil, 290: 259–268. Sirinivas, T., Sridevi, M. & Mallaiah, K.V. 2008. Effect of pesticides on Rhizobium and nodulation of green gram Vigna radita (L.) Wilczek. ICFAI Journal of Life Science, 2: 36–44. Smolinska, U. & Kowalczyk, W. 2014. The impact of the Brassicaceae plant materials added to the soil on the population of Fusarium solani (Mart.) SACC. and Fusarium oxysporum SCHLEKHT. Jounal of Horticultural Research, 22(1):123–129. Soltani Tolarod, A.A., Abbaszadeh Dahaji, P., Khavazi, K., Asadi Rahmani, H. & Shahriari, M.H. 2014. Evaluation of Vitavax ®-34 fungicide biological effects on growth and survival of plant growth promoting rhizobacteria genus Pseudomonas and Azospirilium. Journal of Soil Management and Sustainable production, Vol. 4(1): 235–247. Strullu-Derrien, C., Selosse, M. A., Kenrick, p. and Martin, F. M. 2018. The origin and evolution of mycorrhizal symbioses: from palaeomycology to phylogenomics. New Phtologist Trust, Available in: https://doi.org/10.1111/nph.15076 Tavassolee, A., Aliasgharzad, N., Salehi, G.R., Mardi, M., Asgharzadeh, A. & Akbarivala, S. 2011. Effect of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on fungal occupancy in chickpea root and nodule determined byReal-Time PCR. Current Microbiology, 63:107–114. Vlassak, K. M., Vanderleyden, J. and Graham, D. P. H. 2010. Factors influencing nodule occupancy by inoculant rhizobia. Critical Reviewer in Plant Science, Available in: http://www.tandfonline.com/loi/bpts 20. Wang, Y.Y., Vestberg, M., Walker, C., Hurme, T., Zhang, X. and Lindström, K. 2008: Diversity and infectivity of arbuscular mycorrhizal fungi in agricultural soils of the Sichuan Province of mainland China. Mycorrhiza: 18: 59–68. | ||
|
آمار تعداد مشاهده مقاله: 722 تعداد دریافت فایل اصل مقاله: 1,180 |
||