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بررسی تأثیر تعدادی از سویههای باکتریایی بر واکنشهای دفاعی خیار نسبت به بیماری پوسیدگی فوزاریومی ساقه و ریشه | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 3، دوره 6، شماره 1 - شماره پیاپی 11، شهریور 1397، صفحه 29-42 اصل مقاله (314.63 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2018.119373 | ||
| نویسندگان | ||
| الهام اکبری مقدم؛ روحاله صابری ریسه* ؛ پژمان خدایگان؛ حسین علایی | ||
| گروه گیاهپزشکی دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، رفسنجان، ایران | ||
| چکیده | ||
| خیار یکی از مهمترین محصولات جالیزی ایران است که آفات و عوامل بیماریزا محدودکننده کشت این محصول بوده و منجر به کاهش عملکرد میشوند. مبارزه بیولوژیک بهترین روش مبارزه با بیماری پوسیدگی فوزاریومی ساقه و ریشه است. در این تحقیق اثرات آنتاگونیستی سویههای سودوموناس فلورسنت روی قارچهای مهم پوسیدگی ریشه خیار در آزمایشگاه و گلخانه بررسی شد. از میان جدایههای موردبررسی، جدایهی VUPf760 بیشترین میزان هاله بازدارندگی را علیه قارچهایFusarium oxysporum و Fusarium solani بهترتیب با 9 و 7 میلیمتر نشان داد. همچنین در شرایط گلخانه اثرات بازدارندگی این جدایهها بر بوتهمیری ارزیابی شد. بررسیها نشان داد که تیمار بذرها با جدایههای T17-4و VUPf5 به ترتیب 58 و 60 درصد شدت بیماری حاصل از قارچهای F. oxysporum وF. solani را کاهش دادند. ارزیابی فعالیت آنزیم پراکسیداز، فنل کل و پلیفنل اکسیداز نشان داد که هر دو جدایه قارچ بهتنهایی باعث افزایش فعالیت این آنزیمها در گیاهچه خیار شدند. اما فعالیت این آنزیمها در تیمار باکتری و قارچ بهصورت توأم، از میزان بالاتری برخوردار بود. | ||
| کلیدواژهها | ||
| فوزاریوم؛ بیوکنترل؛ آنزیم؛ فاکتورهای رشدی | ||
| عنوان مقاله [English] | ||
| The effect of some bacteria strains on cucumber defense reactions for controlling of Fusarium stem and root rot disease | ||
| نویسندگان [English] | ||
| Elham Akbari Moghadam؛ Rohollah Saberi-Riseh؛ Pezhman Khodaygan؛ Hossein Alaei | ||
| Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran | ||
| چکیده [English] | ||
| Cucumber is one of the most important crop in Iran. Pests and pathogens are the most important factors limiting its cultivation and lead to reduced yields. Biological control is condiderd as the best control method for soil-borne disease including Fusarium stem and root rot. In this study, antagonistic effects of Pseudomonas fluorescens strains on the important root rot pathogens of cucumber with dual culture method was examined in vitro. Strain VUPf760 showed the highest inhibition zone against Fusarium oxysporium and Fusarium solani with 9 and 7 mm, respectively. Inhibitory effects of these isolates on the disease was evaluated in the greenhouse. The results showed that seeds treatment with T17-4 and VUPf5 isolates reduced the severity of disease caused by F. oxysporum and F. solani by58 and 60%, respectively. In addition, all three isolates alone increased peroxidase activity, total phenols and polyphenol oxidase in cucumber seedlings. However, the activity of these enzymes were higher in treatments with combination of antagonist and pathogen. | ||
| کلیدواژهها [English] | ||
| Fusarium spp, biocontrol, enzyme, growth factors | ||
| مراجع | ||
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Agrios, G.N. 2005. Plant Pathology (5rd ed.). Academic Press, New York ,USA. Ahmad, F., Ahmad, I. & Khan, M. S. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological research, 163: 173-181. Anderws, J. H. 1992. Biological control soil born pathogen agents. Agriculture Training Publication. Banihashemi, Z. & Moradi, M. 2004. The frequency of isolation of Phytophthora spp. from crown and root of pistachio nut tree and reaction of the crown and root to the casual agent. Iranian Journal of Plant Pathology, 40: 57-75. (In Farsi) Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72: 248-254. Cerkauskas, R., Brown, J. & Ferguson, G. (2001) First report of Fusarium stem and root rot of greenhouse cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum in Ontario. Plant disease, 85: 1028-1028. Chen, C., Belanger, R. R., Benhamou, N. & Paulitz, T. C. 2000. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology, 56: 13-23. Etebarian ,H. R. 2002. Vegetable diseases and ways to counter them (2th ed.). Publication of Tehran University. Food and Agriculture Organization. 2012. Statistics Division.from http://faostat3.fao.org/faostat-gateway/. Fujita, S., Saari, N. b., Maegawa, M., Tetsuka, T., Hayashi, N. & Tono, T. 1995. Purification and properties of polyphenol oxidase from cabbage (Brassica oleracea L.). Journal of Agricultural and Food Chemistry, 43: 1138-1142. Hagedorn, C., Gould, W. D. & Bardinelli, T. R. 1989. Rhizobacteria of cotton and their repression of seedling disease pathogens. Applied and Environmental Microbiology, 55: 2793-2797. Harish, S., Kavino, M., Kumar, N., Saravana Kumar, D., Soorianathasundaram, K. and Samiyappan, R. 2008. Bio hardening with plant growth promoting rhizosphere and endophytic bacteria induces systemic resistance against banana bunchy top virus. Applied Soil Ecology, 39: 187-200. Jarvis,W.R. 1992. Cucumber diseases . Reaserch station Harrow, Ontario. Agriculture Canada Publication. Kamilova, F., Validov, S., Azarova, T., Mulders, I. & Lugtenberg, B. 2005. Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environmental microbiology, 7: 1809-1817. Keel, C., Weller, D. M., Natsch, A., Défago, G., Cook, R. J. & Thomashow, L. S. 1996. Conservation of the 2, 4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations. Applied and Environmental Microbiology, 62: 552-563. Khatamidoost Shekarsaraee, Z. 2014. Effect of Pseudomonas fluorescens in control of pistachio root knot nematode in green house conditions. A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science (MSc) in Plant Pathology, University of Guilan, Rasht, Iran. (In Farsi) Kidoglu, F., Gül, A., Ozaktan, H. & Tüzel, Y. 2007. Effect of rhizobacteria on plant growth of different vegetables. In: Proceedings of International Symposium on High Technology for Greenhouse System Management: Greensys2007, Naples, Italy. Kloepper, J. W., Lifshitz, R. & Zablotowicz, R. M. 1989. Free living bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7: 39-44. Li, L. & Steffens, J. C. 2002. Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta, 215: 239-247. MacAdam, J. W., Nelson, C. J. & Sharp, R. E. 1992. Peroxidase Activity in the Leaf Elongation Zone of Tall Fescue I. Spatial Distribution of Ionically Bound Peroxidase Activity in Genotypes Differing in Length of the Elongation Zone. Plant Physiology, 99: 872-878. Mayer, A. M. & Staples, R. C. 2002. Laccase: new functions for an old enzyme. Phytochemistry, 60: 551-565. Moreno, A., Alferez, A., Aviles, M., Dianez, F., Blanco, R., Santos, M. & Tello, J. C. 2001. First report of Fusarium oxysporum f. sp. radicis-cucumerinum on cucumber in Spain. Plant disease, 85: 1206-1206. M'piga, P., Belanger, R. R., Paulitz, T. C. & Benhamou, N. 1997. Increased resistance to Fusarium oxysporum f. sp. radicis-lycopersici in tomato plants treated with the endophytic bacterium Pseudomonas fluorescens strain 63-28. Physiological and Molecular Plant Pathology, 50: 301-320. Murthy, K. N., Uzma, F. & Srinivas, C. C. 2014. Induction of Systemic Resistance in Tomato against Ralstonia solanacearum by Pseudomonas fluorescens. American Journal of Plant Sciences, 5: 1799 - 1811. Nandakumar, R., Babu, S., Viswanathan, R., Raguchander, T. and Samiyappan, R. 2001. Induction of systemic resistance in rice against sheath blight disease by pseudomonas fluorescens. Soil Biology & Biochemistry, 33: 603-612. Ownley, B. H., Duffy, B. K. & Weller, D. M. 2003. Identification and manipulation of soil properties to improve the biological control performance of phenazine-producing Pseudomonas fluorescens. Applied and Environmental Microbiology, 69: 3333-3343. Raj, S.N., Sarosh, B.R. and Shetty, H. S. 2006. Induction and accumulation of polyphenol oxidase activities as implicated in development of resistance against pearl millet downy mildew disease. Functional Plant Biology, 33: 563-571. Raupach, G. S. & Kloepper, J. W. 1998. Mixtures of plant growth- promoting rhizobacteria enhance biological control of multiple excumber pathogens. Journal of Phytopatholoy, 88: 1158- 1164. Reuveni, R. 1995. Biochemical marker for disease resistance. In: R. P., Singh and U. S., Singh (Eds), MolecularMethods in Plant Pathology. (pp. 99-144). CRC Press, Boca Raton. Reverchon, S., Monnet, Y., Beliard, E. & Alabouvette, C. 2000. Du nouveau sur les fusarioses du concombre: Fusarium oxysporum f. sp. radicis-cucumerinum (FORC) isolé pour la première fois en France. Phytoma-La Défense des végétaux, 36-38. Salman, M., Abuamsha, R. & Barghouthi, S. 2013. Interaction of Fluorescent Pseudomonads with Pythium ultimum and Rhizoctonia solani in Cucumber Roots. American Journal of Experimental Agriculture, 3 (1):240-251. Shaharoona, B., Arshad, M., Zahir, Z. A. & Khalid, A. 2006. Performance of Pseudomonas spp. Containing ACC-Deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistry, 38(9): 2971-2975. Shahriyari, D., Zare, R., Hafez, Kh. & Kherkhani, H. 2006. Fusarium root and stem rot of cucumber. In: Proceedings of the 17th Iranian Plant Protection Congress, 2-5 Sep, University of Tehran, Karaj, Iran. (In Farsi). Soland, S. F. & Laima, S. K. 1999. Phenolics and cold tolerance of Brassica napus. Plant Agric, 1:1-5. Spadaro, D. & Gullino, M. L. 2005. Improving the efficacy of biocontrol agents against soilborne pathogens. Crop Protection, 24: 601-613. Steiner, U. & Schönbeck, F. 1995. Induced disease resistance in monocots. In: R., Hammerschmidt & J., Kuc (Eds.), Induced resistance to disease in plants. Springer. Thomashow, L. S. & Weller, D. M. 1988. Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. Journal of bacteriology, 170: 3499-3508. Vakalounakis, D. J. 1996. Root and Stem Rot of Cucumber Caused by Fusarium oxysporum. Plant disease: 313 - 316. Van Loon, L. C. & Van Strien, E. A. 1999. The families of pathogenesis-related proteins, their activities and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology, 55: 85-97. Weller, D. M. (1988) Biological Control of Soilborne Plant Pathogens in the Rhizosphere with Bacteria. Annual review of phytopathology, 26: 379-407. Wildermuth, G. & McNamara, R. 1987. Susceptibility of winter and summer crops to root and crown infection by Bipolaris sorokiniana. Plant pathology, 36: 481-491. Wood, K. R., & Barbara, D. J. 1971. Virus multiplication and peroxidase activity in leaves of cucumber (Cucumis sativus L.) cultivar systemically infected with W strain of cucumber mosaic virus. Physiological Plant Pathology, 1: 73-81. Zahir, Z. A., Arshad, M. & Frankenberger, W. T. 2004. Plant growth promoting rhizobacteria: Application and perspectives in agriculture. Advances in Agronomy, 81: 97-168. | ||
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