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بررسی تأثیر تعدادی از سویه‌های باکتریایی بر واکنش‌های دفاعی خیار نسبت به بیماری پوسیدگی فوزاریومی ساقه و ریشه | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 3، دوره 6، شماره 1 - شماره پیاپی 11، شهریور 1397، صفحه 29-42 اصل مقاله (314.63 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2018.119373 | ||
| نویسندگان | ||
| الهام اکبری مقدم؛ روح‌اله صابری ریسه* ؛ پژمان خدایگان؛ حسین علایی | ||
| گروه گیاه‌پزشکی دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، رفسنجان، ایران | ||
| چکیده | ||
| خیار یکی از مهم‌ترین محصولات جالیزی ایران است که آفات و عوامل بیماری‌زا محدودکننده کشت این محصول بوده و منجر به کاهش عملکرد می‌شوند. مبارزه بیولوژیک بهترین روش مبارزه با بیماری پوسیدگی فوزاریومی ساقه و ریشه است. در این تحقیق اثرات آنتاگونیستی سویه‌های سودوموناس فلورسنت روی قارچ‌های مهم پوسیدگی ریشه خیار در آزمایشگاه و گلخانه بررسی شد. از میان جدایه‌های موردبررسی، جدایه‌ی VUPf760 بیشترین میزان هاله بازدارندگی را علیه قارچ‌هایFusarium oxysporum و Fusarium solani به‌ترتیب با 9 و 7 میلی‌متر نشان داد. همچنین در شرایط گلخانه اثرات بازدارندگی این جدایه‌ها بر بوته‌میری ارزیابی شد. بررسی‌ها نشان داد که تیمار بذرها با جدایه‌های T17-4و VUPf5 به ترتیب 58 و 60 درصد شدت بیماری حاصل از قارچ‌های F. oxysporum وF. solani را کاهش دادند. ارزیابی فعالیت آنزیم پراکسیداز، فنل کل و پلی‌فنل اکسیداز نشان داد که هر دو جدایه قارچ به‌تنهایی باعث افزایش فعالیت این آنزیم‌ها در گیاهچه خیار شدند. اما فعالیت این آنزیم‌ها در تیمار باکتری و قارچ به‌صورت توأم، از میزان بالاتری برخوردار بود. | ||
| کلیدواژه‌ها | ||
| فوزاریوم؛ بیوکنترل؛ آنزیم؛ فاکتورهای رشدی | ||
| عنوان مقاله [English] | ||
| The effect of some bacteria strains on cucumber defense reactions for controlling of Fusarium stem and root rot disease | ||
| نویسندگان [English] | ||
| Elham Akbari Moghadam؛ Rohollah Saberi-Riseh؛ Pezhman Khodaygan؛ Hossein Alaei | ||
| Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran | ||
| چکیده [English] | ||
| Cucumber is one of the most important crop in Iran. Pests and pathogens are the most important factors limiting its cultivation and lead to reduced yields. Biological control is condiderd as the best control method for soil-borne disease including Fusarium stem and root rot. In this study, antagonistic effects of Pseudomonas fluorescens strains on the important root rot pathogens of cucumber with dual culture method was examined in vitro. Strain VUPf760 showed the highest inhibition zone against Fusarium oxysporium and Fusarium solani with 9 and 7 mm, respectively. Inhibitory effects of these isolates on the disease was evaluated in the greenhouse. The results showed that seeds treatment with T17-4 and VUPf5 isolates reduced the severity of disease caused by F. oxysporum and F. solani by58 and 60%, respectively. In addition, all three isolates alone increased peroxidase activity, total phenols and polyphenol oxidase in cucumber seedlings. However, the activity of these enzymes were higher in treatments with combination of antagonist and pathogen. | ||
| کلیدواژه‌ها [English] | ||
| Fusarium spp, biocontrol, enzyme, growth factors | ||
| مراجع | ||
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