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اثر عصاره آبی گیاه آلوئهورا (Aloe vera L.) در بیان گیرنده P75NTR و نوروتروفین BDNF پس از ضایعه فشاری طناب نخاعی در موشهای بزرگ | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 13، دوره 30، شماره 6 - شماره پیاپی 68، بهمن 1393، صفحه 965-976 اصل مقاله (1.24 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.11931 | ||
| نویسندگان | ||
| مرجان حشمتی* 1؛ محمدرضا جلالی ندوشن2؛ شهرزاد فخریه3 | ||
| 1استادیار، گروه علوم تشریح و پاتولوژی، دانشکده پزشکی، دانشگاه شاهد | ||
| 2استاد، گروه علوم تشریح و پاتولوژی، دانشکده پزشکی، دانشگاه شاهد | ||
| 3دانشآموخته پزشکی، دانشکده پزشکی، دانشگاه شاهد | ||
| چکیده | ||
| بهمنظور ارزیابی اثر عصاره آبی آلوئهورا (Aloe vera L.) در حیات سلولهای عصبی حرکتی، بیان گیرنده مرگ (P75NTR و نوروتروفینمنتج از مغز) BDNF مورد بررسی قرار گرفت. 24 موش بزرگ بالغ ماده نژاد اسپراگ داولی از انستیتو رازی تهیه شد. حیوانات به 4 گروه: 1- لامینکتومی (مهره 11-9 پشتی) و تزریق عصاره آبی آلوئهورا، 2- لامینکتومی و تزریق عصاره آبی آلوئهورا به همراه فشار بر نخاع، 3- لامینکتومی و تزریق سرم فیزیولوژی و 4- لامینکتومی و تزریق سرم فیزیولوژی به همراه اعمال فشار بر نخاع تقسیم شدند. تزریقات روزانه و داخل صفاقی به مدت 4 هفته انجام گردید. پس از گذشت 4 هفته موشها کشته شدند. شمارش سلولهای عصبی حرکتی نخاع و مورفومتری انجام شد. بیان گیرنده P75NTR و BDNF به روش ایمنوهیستوشیمیایی بررسی شد. نتایج مطالعات شمارش سلولهای عصبی حرکتی نخاع و مورفومتری، حکایت از کاهش به همراه خونریزی و ایجاد حفره در لابهلای سلولهای بافت نخاع بدنبال فشار مکانیکی داشت. عصاره آبی آلوئهورا سبب کاهش مرگ سلولهای عصبی حرکتی (p ≤ 0.05) به همراه افزایش بیان BDNF گردید (p ≤ 0.05)؛ باوجود افزایش بیان گیرنده P75NTR در گروه دوم، سبب کاهش معنیدار مرگ سلولهای عصبی گردید (p ≤ 0.05) که میتواند ناشی از نقش دوگانه گیرنده و خواص متعدد و متنوع گیاه آلوئهورا باشد. البته اثر ایمنو مدولاتوری نیز بهگونهای نقش متعدد آلوئهورا و حیات یا مرگ سلولهای عصبی در زمانهای مختلف نقش دوگانه گیرنده P75NTR را همانند نتایج تحقیق حاضر مطرح میکند. | ||
| کلیدواژهها | ||
| ضایعات مکانیکی طناب نخاع؛ عصاره آبی آلوئهورا (Aloe vera L.)؛ گیرنده مرگ (P75NTR)؛ نوروتروفینمنتج از مغز (BDNF) | ||
| عنوان مقاله [English] | ||
| Effect of aqueous extract of Aloe vera L. on the expression of 75NTR and BDNF after spinal cord compression in adult rats | ||
| نویسندگان [English] | ||
| M. Heshmati1؛ M.R. Jalali Nadoushan2؛ Sh. Fakhrieh3 | ||
| 1Department of Anatomy and Pathology, Shahed University, Tehran, Iran | ||
| 2Department of Anatomy and Pathology, Shahed University, Tehran, Iran | ||
| 3Medical Graduate, Shahed University, Tehran, Iran | ||
| چکیده [English] | ||
| This research was aimed to evaluate the effects of aqueous extract of Aloe vera L., on motoneuron survival, P75NTR and BDNF expression. Twenty-Four Sprague-Dawley rats were obtained from Razi Institute. They were divided into four groups:1-laminectomy ((T9-T11 vertebra)+injection of Aloe vera extract. 2- laminectomy+injection Aloe vera extract+compression. 3- laminectomy+injection of saline normal. 4- laminectomy+injection of normal saline + compression. Intraprituneal injection continued for four weeks. After four weeks, they were sacrificed. Spinal cord motoneurons were counted and their morphometry was studied. Expression P75NTR and BDNF were analyzed by immunohistochemistry. Results showed that compression caused motoneurons reduction with hematoma among the cells and in cavitation. The aqueous extract of Aloe vera caused to decrease the motoneurons death and increase the BDNF expression (p ≤ 0.05). In the second group, despite increasing expression of P75NTR, motoneuron death decreased significantly (p ≤ 0.05) which may be due to the dual role of the receptor or various properties of Aloe vera. The immunomodulatory effect of Aloe vera and multiple action of P75 in survival or motoneuron death in different times of life were confirmed in this research. | ||
| کلیدواژهها [English] | ||
| Mechanical spinal cord injury, aquae extract of Aloe vera L, P75NTR receptor, BDNF | ||
| مراجع | ||
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