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اثر عصاره آبی گیاه آلوئهورا (Aloe vera L.) در بیان گیرنده P75NTR و نوروتروفین BDNF پس از ضایعه فشاری طناب نخاعی در موشهای بزرگ | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 13، دوره 30، شماره 6 - شماره پیاپی 68، بهمن 1393، صفحه 965-976 اصل مقاله (1.24 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.11931 | ||
| نویسندگان | ||
| مرجان حشمتی* 1؛ محمدرضا جلالی ندوشن2؛ شهرزاد فخریه3 | ||
| 1استادیار، گروه علوم تشریح و پاتولوژی، دانشکده پزشکی، دانشگاه شاهد | ||
| 2استاد، گروه علوم تشریح و پاتولوژی، دانشکده پزشکی، دانشگاه شاهد | ||
| 3دانشآموخته پزشکی، دانشکده پزشکی، دانشگاه شاهد | ||
| چکیده | ||
| بهمنظور ارزیابی اثر عصاره آبی آلوئهورا (Aloe vera L.) در حیات سلولهای عصبی حرکتی، بیان گیرنده مرگ (P75NTR و نوروتروفینمنتج از مغز) BDNF مورد بررسی قرار گرفت. 24 موش بزرگ بالغ ماده نژاد اسپراگ داولی از انستیتو رازی تهیه شد. حیوانات به 4 گروه: 1- لامینکتومی (مهره 11-9 پشتی) و تزریق عصاره آبی آلوئهورا، 2- لامینکتومی و تزریق عصاره آبی آلوئهورا به همراه فشار بر نخاع، 3- لامینکتومی و تزریق سرم فیزیولوژی و 4- لامینکتومی و تزریق سرم فیزیولوژی به همراه اعمال فشار بر نخاع تقسیم شدند. تزریقات روزانه و داخل صفاقی به مدت 4 هفته انجام گردید. پس از گذشت 4 هفته موشها کشته شدند. شمارش سلولهای عصبی حرکتی نخاع و مورفومتری انجام شد. بیان گیرنده P75NTR و BDNF به روش ایمنوهیستوشیمیایی بررسی شد. نتایج مطالعات شمارش سلولهای عصبی حرکتی نخاع و مورفومتری، حکایت از کاهش به همراه خونریزی و ایجاد حفره در لابهلای سلولهای بافت نخاع بدنبال فشار مکانیکی داشت. عصاره آبی آلوئهورا سبب کاهش مرگ سلولهای عصبی حرکتی (p ≤ 0.05) به همراه افزایش بیان BDNF گردید (p ≤ 0.05)؛ باوجود افزایش بیان گیرنده P75NTR در گروه دوم، سبب کاهش معنیدار مرگ سلولهای عصبی گردید (p ≤ 0.05) که میتواند ناشی از نقش دوگانه گیرنده و خواص متعدد و متنوع گیاه آلوئهورا باشد. البته اثر ایمنو مدولاتوری نیز بهگونهای نقش متعدد آلوئهورا و حیات یا مرگ سلولهای عصبی در زمانهای مختلف نقش دوگانه گیرنده P75NTR را همانند نتایج تحقیق حاضر مطرح میکند. | ||
| کلیدواژهها | ||
| ضایعات مکانیکی طناب نخاع؛ عصاره آبی آلوئهورا (Aloe vera L.)؛ گیرنده مرگ (P75NTR)؛ نوروتروفینمنتج از مغز (BDNF) | ||
| عنوان مقاله [English] | ||
| Effect of aqueous extract of Aloe vera L. on the expression of 75NTR and BDNF after spinal cord compression in adult rats | ||
| نویسندگان [English] | ||
| M. Heshmati1؛ M.R. Jalali Nadoushan2؛ Sh. Fakhrieh3 | ||
| 1Department of Anatomy and Pathology, Shahed University, Tehran, Iran | ||
| 2Department of Anatomy and Pathology, Shahed University, Tehran, Iran | ||
| 3Medical Graduate, Shahed University, Tehran, Iran | ||
| چکیده [English] | ||
| This research was aimed to evaluate the effects of aqueous extract of Aloe vera L., on motoneuron survival, P75NTR and BDNF expression. Twenty-Four Sprague-Dawley rats were obtained from Razi Institute. They were divided into four groups:1-laminectomy ((T9-T11 vertebra)+injection of Aloe vera extract. 2- laminectomy+injection Aloe vera extract+compression. 3- laminectomy+injection of saline normal. 4- laminectomy+injection of normal saline + compression. Intraprituneal injection continued for four weeks. After four weeks, they were sacrificed. Spinal cord motoneurons were counted and their morphometry was studied. Expression P75NTR and BDNF were analyzed by immunohistochemistry. Results showed that compression caused motoneurons reduction with hematoma among the cells and in cavitation. The aqueous extract of Aloe vera caused to decrease the motoneurons death and increase the BDNF expression (p ≤ 0.05). In the second group, despite increasing expression of P75NTR, motoneuron death decreased significantly (p ≤ 0.05) which may be due to the dual role of the receptor or various properties of Aloe vera. The immunomodulatory effect of Aloe vera and multiple action of P75 in survival or motoneuron death in different times of life were confirmed in this research. | ||
| کلیدواژهها [English] | ||
| Mechanical spinal cord injury, aquae extract of Aloe vera L, P75NTR receptor, BDNF | ||
| مراجع | ||
|
- Akhtar, A.Z., Pippin, J.J. and Sandusky, C.B., 2009. Animal studies in spinal cord injury: a systematic review of methylprednisolone. Alternatives to Laboratory Animals, 37: 43-62. - Ameloot, P., Fiers, W., De Bleser, P., Ware, C.F., Vandenabeele, P. and Brouckaert, P., 2001. Identification of tumor necrosis factor (TNF) amino acids crucial for binding to the murine P75 TNF receptor and construction of receptor-selective mutants. The Journal of Biological Chemistry, 276(40): 37426-37430. - Bancroft, J.D. and Stevens, A., 1990. Theory and Practice of Histological Techniques. Churchill Livingstone, Londen, 740p. - Boyd, J.G. and Gordon, T., 2002. A dose-dependent facilitation and inhibition of peripheral nerve regeneration by brain-derived neurotrophic factor. European Journal of Neuroscience, 15(4): 613-626 - Chopp, M., Zhang, X.H., Li, Y., Wang. L., Chen, J., Lu, D., Lu, M. and Rosenblum, M., 2000. Spinal cord injury in rat: treatment with bone marrow stromal cell transplantation. Neuro Report, 11(13): 3001-3005. - Crowe, M.J., Bresnahan, J.C., Shuman, S.L., Masters, J.N. and Beattie, M.S., 1997. Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nature Medicine, 3: 73-76. - DeFreitas, M.F., McQuillen, P.S. and Shatz, C.J., 2001. A novel P75NTR signaling pathway promotes survival, not death, of immunopurified neocortical subplate neurons. The Journal of Neuroscience, 21(14): 5121-5129. - Dolan, E.J. and Tator, C.H., 1979. A new method for testing the force of clips for aneurysms or experimental spinal cord compression. Journal of Neurosurgery, 51(2): 229-233. - Dolan, E.J., Tator, C.H. and Endrenyi, L., 1995. The value of decompression for acute experimental spinal cord compression injury. Journal of Neurosurgery, 53(6): 749-755. - Farahnejad, Z., Ghazanfari, T. and Yaraee, R., 2011. Immunomodulatory effects of Aloe vera and its fractions of macrophages against Candida albicans. Immunopharmacology and Immunotoxicology, 33(4): 676-681. - Friedman, W.J., 2000. Neurotrophins induce death of hippocampal neurons via the P75 receptor. The Journal of Neuroscience, 20(17): 6340-6346. - Hains, B.C., Yucra, J.A. and Hulsebosch, C.E., 2001. Reduction of pathological and behavioral deficits following spinal cord contusion injury with the selective cyclooxygenase-2 inhibitor NS-398. Journal of Neurotrauma, 18(4): 409-423. - Hajebrahimi, Z., Mowla, S.J., Movahedin, M. and Tavallaei, M., 2008. Gene expression alterations of neurotrophins, their receptors and prohormone convertase in a rat model of spinal cord contusion. Neuroscience Letters, 441(3): 261-266. - Heshmati, M., Jalali Nadoushan, M.R., Entezari, E. and Khodashenas, Z., 2013. The effect of aquae extract of Aloe vera on synaptophysin expression after spinal cord compression in adult rats. Journal of Medicinal Plants, 12(46): 38-46. - Hulsebosch, C.E., 2002. Recent advances in pathophysiology and treatment of spinal cord injury. Advances in Physiology Education, 26(1-3): 238-255. - Ibanez, C.F. and Simi, A., 2012. Review-P75 neurotrophin receptor signaling in nervous system injury and degeneration: paradox and opportunity. Trends in Neurosciences, 35(7): 431-440. - Ichim, G., Tauszig-Delamasure, S. and Mehlen, P., 2012. Neurotrophins and cell death. Experimental Cell Research, 318(11): 1221-1228. - Jacobson, M., Weil, M. and Raff, M.C., 1997. Programmed cell death in animal development. Cell, 88(3): 347-354. - Le, T.H. and Gean, A.D., 2009. Neuroimaging of traumatic brain injury. The Journal of Spinal Cord Medicine, 76(2): 145-162. - Liu, X.Z., Xu, X.M., Hu, R., Du, C., Zhang, S.X., McDonald, J.W., Dong, H.X., Wu, Y.J., Fan, G.S., Jacquin, M.F., Hsu, C.Y. and Choi, D.W., 1997. Neuronal and glial apoptosis after traumatic spinal cord injury. The Journal of Neuroscience, 17(14): 5395-5406. - Longo, F.M. and Massa, S.M., 2008. Small molecule modulation of p75 neurotrophin receptor functions. CNS & Neurological Disorders-Drug Targets, 7: 63-70. - Loots du, T., van der Westhuizen, F.H. and Botes, L., 2008. Aloe ferox leaf gel phytochemical content, antioxidant capacity, and possible health benefits. Journal of Agricultural and Food Chemistry, 55(17): 6891-6896. - Sadel, F., Bechade, C. and Triller, A., 1999. Nerve growth factor induces motoneuron apoptosis in rat embryonic spinal cord in vitro. European Journal of Neuroscience, 11(11): 3904-3912. - Scott, A.L.M. and Ramer, M.S., 2010. Differential regulation of dendritic plasticity by neurotrophins following deafferentation of the adult spinal cord is independent of p75(NTR). Brain Research, 1323: 48-58. - Singh, K.K., Park, K.J., Hong, E.J., Kramer, B.M., Greenberg, M.E., Kaplan, D.R. and Miller, F.D., 2008. Developmental axon pruning mediated by BDNF-p75 NTR-dependent axon degeneration. Nature Neuroscience, 11(6): 649-658. - Song, X.Y., Zhong, J.H., Wang, X. and Zhou, X.F., 2004. Suppression of p75ntr does not promote regeneration of injured spinal cord in mice. The Journal of Neuroscience, 24(2): 542-546. - Song, W., Volosin, M., Cragnolini, A.B., Hempstead, B.L. and Friedman, W.J., 2010. ProNGF induces PTEN via p75NTR to suppress Trk-mediated survival signaling in brain neurons. The Journal of Neuroscience, 30(46): 15608-15615. - Taylor, A.R., Gifondorwa, D.J., Robinson, M.B., Strupe, J.L., Prevette, D., Johnson, J.E., Hempstead, B., Oppenheim, R.W. and Milligan, C.E., 2012. Motoneuron programmed cell death in response to proBDNF. Developmental Neurobiology, 72(5): 699-712. - Widenfalk, J., Lundströmer, K., Jubran, M., Brene, S. and Olson, L., 2001. Neurotrophic factors and receptors in the immature and adult spinal cord after mechanical injury or kainic acid. Journal of Neuroscience, 21(10): 3457-3475. - Xie, Y., Yao, Z., Chai, H., Wong, W.M. and Wu, W., 2003. Expression and role of low-affinity nerve growth factor receptor (p75) in spinal motor neurons of aged rats following axonal injury. Developmental Neurobiology, 25: 65-71. - Yu, M., Bergman, E., Edstrom, E. and Ulfhake, B., 1999. Reciprocal changes in the expression of neurotrophin mRNA target tissues and peripheral nerves of aged rats. Neuroscience Letters, 273(3): 187-90. - Zagrebelsky, M., Holz, A., Dechant, G., Barde, Y.A., Bonhoeffer, T. and Korte, M., 2005. The p75 neurotrophin receptor negatively modulates dendrite complexity and spine density in hippocampal neurons. The Journal of Neuroscience, 25(43): | ||
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