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معرفی چند ژنوتیپ و رقم سیبزمینی مقاوم به ویروس برگ قاشقیسیبزمینی (PLRV) | ||
| آفات و بیماری های گیاهی | ||
| مقاله 4، دوره 86، شماره 2 - شماره پیاپی 107، اسفند 1397، صفحه 163-169 اصل مقاله (134.68 K) | ||
| نوع مقاله: مدیریت آفات و بیماریهای گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2018.121165.1215 | ||
| نویسندگان | ||
| جعفر نیکان* 1؛ رضا پوررحیم2 | ||
| 1عضو هیئت علمی مرکز تحقیقات همدان | ||
| 2عضو هیات علمی بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی کشور | ||
| چکیده | ||
| بیماری برگ قاشقی سیبزمینی یکی از مهمترین و شایعترین بیماریهای ویروسی سیبزمینی است و مانند سایر ویروسهای بیماریزای گیاهی مؤثرترین روش مبارزه با آن استفاده از ارقام مقاوم است. در این تحقیق واکنش تعدادی رقم و ژرمپلاسم سیبزمینی در برابر آلودگی به ویروس برگ قاشقی سیبزمینی (Potato leafroll virus= PLRV) در یک آزمایش مزرعهای در قالب طرح بلوکهای کامل تصادفی با 12 تیمار و سه تکرار ارزیابی شد. مایهزنی بوتههای سیبزمینی با استفاده از شته سبز هلو حامل PLRV صورت گرفت. یک ماه پس از مایه زنی، بوتهها از نظر آلودگی به PLRV با استفاده از علایم ظاهر شده و آزمون الایزا بررسی شد. نتایج نشان داد که بین ژنوتیپهای سیبزمینی مورد بررسی از نظر میزان ابتلا به PLRV اختلاف معنیدار وجود دارد. ژرمپلاسم شماره 13/803970 بدون هیچگونه آلودگی بهعنوان ژنوتیپ خیلی مقاوم به PLRV ارزیابی گردید. رقم سانته مقاوم بود، رقم لیدیرزتا و ژنوتیپ شماره 31/397015 نسبتاً مقاوم و رقم دیامانت نسبتاً حساس بود. مابقی ارقام و ژرمپلاسمها حساس یا بسیار حساس ارزیابی شدند. همچنین نتایج بیانگر همبستگی معنیدار 79 درصدی بین میزان آلودگی بر مبنای ظهور علایم و میزان آلودگی بر مبنای آزمون الایزا بود. | ||
| کلیدواژهها | ||
| الایزا؛ ژرمپلاسم؛ واکنش؛ Potato leafroll virus | ||
| عنوان مقاله [English] | ||
| Introducing some Potato leafroll virus resistant potato genotypes and cultivars | ||
| نویسندگان [English] | ||
| Reza Pourrahim2؛ | ||
| 2Plant Disease Research Department, Iranian Research Institute of Plant Protection, P.O. Box: 1452, Tehran 19395, Iran Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Potato leaf roll disease is one of the most important and widely distributed viral diseases of potato. Like other plant viruses, the use of resistant cultivars is the most effective control measure of this disease. In this study, the reactions of some potato cultivars and genotypes to Potato leafroll virus (PLRV) were evaluated in a field trial experiment. The experiment conducted as a randomized complete block design with 12 treatments and three replications. Each plot of the experimental design included a planting row of five potato plants of each cultivar/genotype. The experimental plants were then inoculated with the virus by putting 10 PLRV-carrying green peach aphids on each plant. One month after inoculation, the plants were examined for PLRV infection by observing symptoms development and using enzyme-linked immunosorbent assay (ELISA) test. The results revealed significant differences between the PLRV-infection rates of the potato cultivars/genotypes tested. The potato genotype 803970/13 with having no infected plant was evaluated as highly resistant to PLRV. The cultivar “Sante” was resistant, the cultivar “Lady Rosetta” and the genotype “397015/31” were moderately resistant, cultivar “Diamant” was moderately susceptible and the rest of genotypes or cultivars were found susceptible or highly susceptible. The results also showed a significant correlation (79%) between the infection rates of the test plants based on symptom development and those of the ELISA tests. | ||
| کلیدواژهها [English] | ||
| ELISA, field trial, PLRV, reaction, resistant | ||
| مراجع | ||
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