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saghafi, K., Ahmadi, J., Asgharzadeh, A., Roknizadeh, H., Hosseini Mazinani, S. (2019). Characterization of Pseudomonas fluorescens bacteria isolated from Olea europaea rhizosphere in Saline Soils. , 7(1), 13-27. doi: 10.22092/sbj.2019.118991
K. saghafi; J. Ahmadi; A. Asgharzadeh; H. Roknizadeh; S. M. Hosseini Mazinani. "Characterization of Pseudomonas fluorescens bacteria isolated from Olea europaea rhizosphere in Saline Soils". , 7, 1, 2019, 13-27. doi: 10.22092/sbj.2019.118991
saghafi, K., Ahmadi, J., Asgharzadeh, A., Roknizadeh, H., Hosseini Mazinani, S. (2019). 'Characterization of Pseudomonas fluorescens bacteria isolated from Olea europaea rhizosphere in Saline Soils', , 7(1), pp. 13-27. doi: 10.22092/sbj.2019.118991
saghafi, K., Ahmadi, J., Asgharzadeh, A., Roknizadeh, H., Hosseini Mazinani, S. Characterization of Pseudomonas fluorescens bacteria isolated from Olea europaea rhizosphere in Saline Soils. , 2019; 7(1): 13-27. doi: 10.22092/sbj.2019.118991

Characterization of Pseudomonas fluorescens bacteria isolated from Olea europaea rhizosphere in Saline Soils

زیست شناسی خاک
Article 3, Volume 7, Issue 1, May 2019, Pages 13-27    PDF (528.38 K)
Document Type: Research Paper
DOI: 10.22092/sbj.2019.118991
Authors
K. saghafi1; J. Ahmadi* 2; A. Asgharzadeh3; H. Roknizadeh4; S. M. Hosseini Mazinani* 5
1Ph.D Student, Imam Khomeini International University, Qazvin, Iran
2Professor. Dept. of Genetic and plant breeding Imam Khomeini International University;Qazvin, Iran
3Associate Professor. Soil and Water Research Institute Agricultural Research, Education and Extension Organization, Karaj, Iran
4Assistant Professor. Dept. of medical Zanjan University of Medical Sciences; Zanjan, Iran
5Professor. Dept. of plant Molecular Genetic National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract
Pseudomonas bacteria are the most important plant growth promoting rhizobacteria due to their widespread distribution in soil, their potential to colonizemany plant roots and possessing different plant growth promoting characteristics. The present study aimed to identify isolates of Pseudomonas fluorescens strains of olive tree rhizosphere in saline gardens of Qazvin province.  Plant growth promoting characteristics of  fifteen isolates of Pseudomonas fluorescens strains such as siderophore, auxin, hydrogen cyanide, ACC deaminase enzyme production, phosphorus solubility in liquid medium, pH changes and salt tolerance (NaCl, MgCl2 and CaCl2) (100, 200, 400 mM) were evaluated . Results showed that the dominant strains in producing ACC deaminase were Q7 and Q9, respectively. The Q2 strain was the best one to enhance the production of auxin (11.96 µg.ml-1). The solubility of phosphorus in conjunction with pH showed a negative correlation and the highest amount of dissolved phosphorus was related to Q13 (251) isolate. Also, the half of strains (40%) was not able to produce HCN. Commonly, all studied strains were able to produce siderophore after two, four and six days of incubation. Expect Q1 and Q8, other strains were able to grow in the considered salt concentrations. Based on the results, we concluded that some of these strains can be targeted for genomics studies to improve salt stress resistance in plants and orchards under saline soils conditions. 
Keywords
ACC deaminase; Auxin; Olive; Siderophore; molecular detection; phylogeny tree
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