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واکنش‌های فیزیولوژیکی و فعالیت آنزیم‌های آنتی‌اکسیدان در برخی از ارقام انگور در شرایط تنش گرما | ||
| به زراعی نهال و بذر | ||
| مقاله 4، دوره 34، شماره 2، آذر 1397، صفحه 171-189 اصل مقاله (261.98 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/sppj.2018.118943 | ||
| نویسندگان | ||
| محمد جواد کرمی* 1؛ سعید عشقی2 | ||
| 1بخش تحقیقات اصلاح و تهیه نهال و بذر استان فارس. سازمان تحقبقات، آموزش و ترویج کشاورزی ایران. | ||
| 2بخش گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران | ||
| چکیده | ||
| به دلیل توسعه کاشت انگور در مناطق گرم در سال‌های اخیر، بررسی واکنش ارقام انگور به تنش گرما به منظور غربالگری و گزینش ارقام متحمل به گرما ضروری است.این پژوهش با هدف تعیین نقش آنزیم‌های آنتی‌اکسیدان شامل: کاتالاز، پراکسیداز، آسکوربات پراکسیداز و سوپراکسید دیسموتاز در فرآیند تحمل گرما در شش رقم انگور تجاری در شرایط تنش گرما (1 ± 45درجه سلسیوس) در موستان در سال‌های 93-1390 در منطقه نیمه گرمسیر قیر در استان فارس انجام شد. شاخص‌های فیزیولوژیکی شامل: محتوای کل کلروفیل، کلروفیل a، کلروفیل b و فلورسانس کلروفیل (Fv/Fm) برگ نیز بررسی شدند. نتایج نشان داد که آنزیم‌های کاتالاز و پراکسیداز فعالیت بالایی در تاک‌ها در مقابل تنش گرما داشتند. در شرایط یکسان تنش گرما بیشترین فعالیت آنزیم‌های کاتالاز و پراکسیداز در تاک‌های فلیم‌سیدلس و رطبی در مقایسه با سایر ارقام مشاهده شد. مقدار بیشتر محتوای کل کلروفیل، کلروفیل a و کلروفیل b با نسبت بالای Fv/Fm برگ همراه بود و مقدار آنها در رقم فلیم‌سیدلس و بعد از آن در رقم رطبی مشاهده شد. نتایج نشان داد رقم فلیم‌سیدلس و پس از آن رقم رطبی از سایرارقام واجد شاخص‌های فیزیولوژیک و بیوشیمیایی مرتبط با سازگاری به شرایط تنش گرما بودند. همخوانی این نتایج با آزمایش مزرعه‌ای قبلی که درآن بالاترین عمکرد را در شرایط تنش گرما برای ارقام رطبی و فلیم سیدلس گزارش شده بود، قابلیت بررسی صفات فیزیولوژیک و فعالیت آنزیم‌های آنتی اکسیدان برای تعیین و غربالگری ارقام متحمل به تنش گرما را نشان داد. | ||
| کلیدواژه‌ها | ||
| انگور رطبی؛ انگور فلیم‌سیدلس؛ پراکسیداز؛ تحمل گرما؛ کاتالاز | ||
| عنوان مقاله [English] | ||
| Physiological Responses and Antioxidant Enzymes Activity in Some Grapevine Cultivars under Heat Stress Conditions | ||
| نویسندگان [English] | ||
| Mohammad Javad Karami1؛ S. Eshghi2 | ||
| 1Deparment of Seed and Plant Improvement of Fars province. | ||
| 2Faculty of Agriculture, University of Shiraz, Shiraz, Iran. | ||
| چکیده [English] | ||
| In recent years, grape growing areas has extended to warm region, therefore, study on grapevine response to heat stress for screening and selection of heat tolerant cultivars is important. Antioxidant systems activity in plants is one of the most important plant adaptation mechanisms to abiotic stresses. This research aimed to determine the response of antioxidant enzymes such as catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and superoxide dismutase (SOD) activities to heat stress in six grapevine cultivars under vineyard conditions. Some physiological traits including; total leaf chlorophyll, chlorophyll a and b contents and chlorophyll fluorescence (Fv/Fm) response were also measured. Results showed that CAT and POD enzymes played criticals roles in the grapevine defense system under high temperature stress. Flame Seedless and Rotabi cultivars had high CAT and POD enzymes activities. Total chlorophyll, chlorophyll a and b concentration were associated to higher Fv/Fm, and were the highest in Flame Seedless followed by Rotabi cultivar. It was concluded that the highest levels of physiological parameters and antioxidant enzymes activities involved in heat stress tolerance in Flame Seedless and Rotabi cultivars, respectively. Similarity between results of this research and previous study under heat stress conditions in which Flame Seedless and Rotabi cultivars had the highest yield showed that physiological traits and antioxidant enzymes activity have the potential for screening grapevine cultivars for heat stress tolerance. | ||
| کلیدواژه‌ها [English] | ||
| Rotabi grape, Flame Seedless grape, peroxidase, heat tolerance, catalase | ||
| مراجع | ||
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