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ارزیابی تنوع ژنتیکی برخی از ژنوتیپهای جنس کنار (Ziziphus) با استفاده از ویژگیهای ریختشناسی و نشانگرهای مولکولی | ||
| نهال و بذر | ||
| مقاله 6، دوره 34، شماره 2، شهریور 1397، صفحه 223-243 اصل مقاله (342.7 K) | ||
| شناسه دیجیتال (DOI): 10.22092/spij.2018.118836 | ||
| نویسندگان | ||
| الهام نوروزی1؛ جواد عرفانی مقدم* 2؛ آرش فاضلی3 | ||
| 1دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران. | ||
| 2استادیار، گروه علوم باغبانی-دانشکده کشاورزی- دانشگاه ایلام- ایران | ||
| 3استادیار، دانشکده کشاورزی، دانشگاه ایلام، ایلام ایران. | ||
| چکیده | ||
| کنار و عناب (Ziziphus spp.) از میوههای گرمسیری و نیمهگرمسیری میباشند. در پژوهش حاضر 72 نمونه از پنج گونهی متعلق به جنس Ziziphus که از بخشهای مختلف استان خوزستان و ایلام جمعآوری شده بودند بر اساس صفات برگ و میوه ارزیابی شدند. نتایج ارزیابی خصوصیات ریختی نشان داد تنوع بالایی در برخی از صفات مانند وزن میوه، نسبت گوشت به بذر، اندازه برگ، نسبت قند به اسید میوه وجود داشت. در میان نمونهها بیشترین و کمترین وزن میوه به ترتیب متعلق به دو گونه Z. mauritiana و Z. nummularia بود. دامنه تغییرات وزن میوه در این پژوهش بین 96/0 تا 46/21 گرم بدست آمد. همچنین، در بخش دوم این پژوهش 30 نمونه از میان ژنوتیپهای بررسی شده که دارای وزن میوه بیشتر بودند انتخاب و تنوع ژنتیکی میان آنها با 15 آغازگر ISSR بررسی شد. در مجموع 166 آلل از 15 مکان ISSR شناسایی و اندازه آللها در محدوده 170 تا 1500 جفتباز متغیر بود. تعداد آللهای مشاهده شده برای هر مکان در دامنه 6 (LBMB-B) تا 15 (HB12 و HB14) آلل با میانگین 06/11 آلل برای هر مکان بود. شاخص محتوای اطلاعات چندشکلی برای مکانهای HB14 و UBC-826 بالا (81/0) و برای مکان LBMB-B دارای کمترین مقدار (65/0) و میانگین آن 76/0 در بین همه مکانهای ISSR بود. | ||
| کلیدواژهها | ||
| کنار؛ عناب؛ منابع ژنتیکی؛ وزن میوه؛ آغازگر ISSR | ||
| عنوان مقاله [English] | ||
| Evaluation of Genetic Diversity in Some Ziziphus Genotypes Using Morphological Characteristics and Molecular Markers | ||
| نویسندگان [English] | ||
| E. Norozi1؛ Javad Erfani-Moghadam2؛ A. Fazeli3 | ||
| 1Former M. Sc. Student, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
| 2Assistant Professor, Department of Horticulture,Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
| 3Assistant Professor, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
| چکیده [English] | ||
| Ber and jujube (Ziziphus spp.) are tropical and subtropical fruits. Seventy two accessions from five Ziziphus species including: Z. jujuba, Z. nummularia, Z. spina-christi, Z. mauritiana and Z. oxyphylla collected from different parts of Khoozestan and Ilam proviences were evaluated using leaf and fruit characteristics. The primary results of morphological characteristics showed that there were high variability for some of the evaluated traits such as fruit weight, pulp:seed ratio, leaf size and TSS/TA ratio. The highest and lowest fruit weight were recorded in Z. mauritiana and Z. nummularia species. In the second experiment, 30 accessions from evaluated germplasm which had higher fruit weight were selected and their genetic diversity was investigated using 15 ISSR primers. A total of 166 alleles were identified by the 15 ISSR primers with their sizes ranging from 170 to 1500 bp. The number of observed alleles for each locus ranged from 6 (LBMB-B) to 15 (HB14, HB12) with an average of 11.06 alleles per locus. Polymorphic information content (PIC) was highest (0.81) in the HB14 and UBC-826 loci, while the LBMB-B locus had the lowest value (0.65), with an average of 0.76 among all ISSR loci. | ||
| کلیدواژهها [English] | ||
| Ber, jujube, genetic resources, fruit weight, ISSR, primer | ||
| مراجع | ||
|
Abdollahi, F., Jafari, L., and Gordi Takhti, S. 2013. Effect of GA3 on growth and chemical composition of jujube leaf (Ziziphus spina-christi) under salinity condition. Journal of Plant Process and Function 2: 53-67 (in Persian).
Asare, M. H. 2008. Biological characteristic of ber trees in Iran. Research Institute of Forest and Rangelands Publication. 594 pp (in Persian).
Azam-Ali, S., Bonkoungou, E., Bowe, C., DeKock, C., Godara, A., and Williams, J. T. 2001. Fruits for the future 2, ber and other jujubes (Revised Edition). International Centre for Underutilised Crops, University of Southampton, Southampton, SO171 BJ, UK. 289 pp.
Bina, F., Zamani, Z., and Nazeri, V. 2012. Morpho-based genetic variation in Christ's thorn (Ziziphus spina-christi (L.) Wild.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 19: 274-288 (in Persian).
Chen, Y. Q., Zhong, X. H., and Gan, L. F. 2006. The performance of Liuxiangzao jujube cultivar and its cultural techniques. South China Fruits 1: 49-50.
Doyle, J. J., and Doyle, J. L. 1990. Isolation of plant DNA form fresh tissue. Focus 12: 13-15.
Fenton, G. A., and Kennedy, M. J. 1998. Rapid dry weight determination of kiwifruit pomace and apple pomace using an infrared drying technique. New Zealand Journal of Crop and Horticultural Science 26: 35-38.
Gao, Q. H., Wu, C. S., Wang, M., Xu, B. N., and Du, L. J. 2012. Effect of drying of jujubes (Ziziphus jujuba Mill.) on the contents of sugars, organic acids, α-tocopherol, β-carotene, and phenolic compounds. Journal of Agricultural and Food Chemistry 60: 9642-9648.
Gao, W. H., Li, X. G., and Wang, C. Z. 2009. Variation in morphology of jujube ‘Muzao’ (Ziziphus jujuba Mill.) in the Losses Plateau of China. Acta Horticulturae 840: 197-202.
Ghazaeian, M., and Zeraatgar, H. 2014. Reproductive and vegetative characteristics of Ziziphus jujube ecotypes of Golestan province. Seed and Plant Improvment Journal 30: 849-855 (in Persian).
Ghosh, S. N., and Mathew, B. 2002. Performance of nine ber (Ziziphus mauritiana Lamk) cultivars on topworking in the semi-arid region of West Bengal. Journal of Applied Horticulture 4: 49-51.
Ghouth, K., Malekzadeh Shafaroodi, S., Rashed Mohassel, M. H., Akbari, M. R., and Razavi, S. H. 2014. Grouping jujubes of Iran based on quantitative characteristics and ISSR and RAPD markers. Seed and Plant Improvment Journal 30: 173-190 (in Persian).
Godt, M. J. W., Race, T., and Hamrick, J. L. 1997. A population genetic analysis of Ziziphus celata, an endangered Florida shrub. Journal of Heredity 88: 531-533.
Grygorieva, O., Abrahamova, V., Karnatovska, M., Bleha, R., and Brindza, J. 2014. Morphological characteristic of fruit, drupes and seeds genotypes of Ziziphus jujuba Mill. Potravinarstvo 8: 306-314.
Jiang, X. W., Cao, J. Q., Zeng, J. X., and Huang, F. P. 2006. Jujube cultivars trials and study on their adaptability. South China Fruits 1: 51-52.
Khakdaman, H., Pourmeidani, A., and Adnani, S. M. 2007. Study of genetic variation in Iranian jujube (Zizyphus jujuba Mill.) ecotypes. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 14: 202-214.
Li, H. T., Li, Z. S., Feng, J. C., Li, J. D., and Bi, H. T. 2008. Genetic analysis of Ziziphus jujuba 'Huizao' using ISSR markers. Acta Horticulturae 840: 135-142.
Ma, Q. H., Wang, G. X., and Liang, L. S. 2011. Development and characterization of SSR markers in Chinese jujube (Ziziphus jujuba Mill.) and its related species. Scientia Horticulturae 129: 597-602.
Mashmoul, A., Hassani, A., Pirzad, A., and Azimi, J. 2013. Effect of chemical and biological phosphorus on the some morphological characters of summer savory (Satureja hortensis). International Journal of Plant Production 4: 184-188.
Mozaffarian, V. 2004. Trees and Shrubs of Iran. Farhang Moaser Publications. 1120 pp. (in Persian).
Obeed, R. S., Harhash, M. M., and Abdel-Mawgood, A. L. 2008. Fruit properties and genetic diversity of five ber (Ziziphus mauritiana Lamk) cultivars. Pakistan Journal of Biological Sciences 11: 888-893.
Paganova, V. 2003. Taxonomical reliability of leaf and fruit morphological characteristic of the Pyrus L. taxa in Slovakia. HortScience 3: 98-107.
Peng, J. Y., Shu, H. R., and Peng, S. Q. 2002. To address the problem of infraspecific classification of Ziziphus jujba Mill. using RAPD data. Acta Phytotaxonomica Sinica 40: 89-94.
Razi, M. F. U. D., Anwar, R., Basra, S. M. A., Khan, M. M., and Khan, I. A. 2013. Morphological characterization of leaves and fruit of jujube (Ziziphus mauritiana Lamk.) germplasm in Faisalabad, Pakistan. Pakistan Journal of Biological Sciences 50: 211-216.
Saeidi, K., Shahhoseini, R., Tavakoli Neko, H., and Saadatjou, B. 2016. Evaluation of some phytochemical, morphological characteristics and minerals content in different populations of jujube (Ziziphus jujaba Mill.). Iranian Journal of Medicinal and Aromatic Plants 32: 245-255.
Saha, D., Srivastava, S. C., and Ramani, R. 2013. Genetic relationships among fruit cultivars and host plants of Indian lac insect in ber (Ziziphus mauritiana Lam.) revealed by RAPD and ISSR markers. Indian Journal of Biotechnology 12: 170-177.
Shahhoseini, R., Babaei, A., Kazemi, M., and Omidbaigi, R. 2012. A study on genetic variation in Iranian jujube (Zizyphus jujuba Mill.) genotypes using molecular AFLP marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 20: 55-68. (in Persian).
Singh, A. K., Sharma, P., Singh, R., Singh, B., Koundal, K. R., and Singh, N. K. 2007. Assessment of genetic diversity in Ziziphus mauritiana using inter-simple sequence repeat markers. Journal of Plant Biochemistry and Biotechnology 16: 35-40.
Wei, X., Sykes, S. R., and Clingeleffer, P. R. 2002. An investigation to estimate genetic parameters in CSIRO’s table grape breeding program. 2. Quality characteristics. Euphytica 128: 343–351.
Zhang, Z., Gao, J., Kong, D., Wang, A., Tang, S., Li, Y., and Pang, X. 2015. Assessing genetic diversity in Ziziphus jujuba ‘Jinsixiaozao’using morphological and microsatellite (SSR) markers. Biochemical Systematics and Ecology 61: 196-202.
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