| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,230 |
| تعداد مقالات | 35,784 |
| تعداد مشاهده مقاله | 54,005,818 |
| تعداد دریافت فایل اصل مقاله | 93,165,909 |
Nucleotide Sequence Analysis of S1 Gene among Iranian Avian Infectious Bronchitis Viruses Isolated during 2001-2002 | ||
| Archives of Razi Institute | ||
| مقاله 3، دوره 74، شماره 1، خرداد 2019، صفحه 21-31 اصل مقاله (706.98 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.123468.1254 | ||
| نویسندگان | ||
| A. Salarpour1؛ R. Toroghi* 1؛ R. Momayez2 | ||
| 1Department of Veterinary Research and Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Mashhad, Iran | ||
| 2Department of Poultry Disease Research and Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran | ||
| چکیده | ||
| Infectious bronchitis (IB) virus genome codes for four structural proteins, among which the S1 subunit of spike glycoprotein comprises the major epitopes to induce neutralizing antibodies. This study involved the comparison of the full S1 sequences of five IB viruses, namely two Massachusetts and three 793/B serotypes, isolated from IB outbreaks during 2001-2002, with all other Iranian and foreign 793/B isolates and 10 known serotypes. Analysis of S1 subunit showed three unique amino acid changes at positions 349 (V to L), 392 (T to N), and 393 (Q or R to T or K or S) for the Iranian 793/B isolates, compared to those of the foreign 793/B isolates reported before 2006 (onset of vaccination with 793/B vaccine in Iran). They were used as amino acid markers for the differentiation of Iranian 793/B isolates for years. Sequence alignment of the Iranian isolates with those of the foreign ones reported after 2006 demonstrated that amino acids 392 and 393 were no longer considered as amino acid markers, and only the change in amino acid 349 still remained specific to the Iranian 793/B isolates. Phylogenetic tree sequence analysis revealed that the Iranian 793/B isolates were closely related indicating that they came from a single source, more probably from France. There was a very close correlation between the first detection of 793/B serotype and the time of French chicken meat importation. Moreover, it was shown that one of the Massachusetts isolates was completely identical with the H120 vaccine strain. Furthermore, the other Massachusetts isolate with two amino acid changes at positions 64 (G to E) and 95 (S to R) was very similar to this vaccine strain. It seems that the latter isolate is a passaged chicken H120 vaccine strain. | ||
| کلیدواژهها | ||
| Infectious bronchitis virus؛ 793/B serotype؛ S1 gene؛ Amino acid marker؛ Phylogenetic analysis | ||
| مراجع | ||
|
Bijanzad, P., Momayez, R., Bozorgmehrifard, M.H., Hablolvarid, M.H., Pourbakhsh, S.A., 2013. Experimental study on histopathological changes and tissue tropism of Iranian infectious bronchitis serotype 793/B-like virus in SPF chickens. J S Afr Vet Assoc 84, E1-7.
Callison, S.A., Jackwood, M.W., Hilt, D.A., 2001. Molecular characterization of infectious bronchitis virus isolates foreign to the United States and comparison with United States isolates. Avian Dis 45, 492-499.
Cavanagh, D., 1983. Coronavirus IBV: structural characterization of the spike protein. J Gen Virol 64 (Pt 12), 2577-2583.
Cavanagh, D., 2007. Coronavirus avian infectious bronchitis virus. Vet Res 38, 281-297.
Cavanagh, D., Davis, P.J., Cook, J.K., Li, D., Kant, A., Koch, G., 1992. Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathol 21, 33-43.
Hosseini, H., Fard, M.H.B., Charkhkar, S., Morshed, R., 2015. Epidemiology of Avian Infectious Bronchitis Virus Genotypes in Iran (2010–2014), SPIE.
Ignjatovic, J., Sapats, S., 2005. Identification of previously unknown antigenic epitopes on the S and N proteins of avian infectious bronchitis virus. Archives of Virology 150, 1813-1831.
Jackwood, M.W., Hilt, D.A., Callison, S.A., Lee, C.W., Plaza, H., Wade, E., 2001. Spike glycoprotein cleavage recognition site analysis of infectious bronchitis virus. Avian Dis 45, 366-372.
Kant, A., Koch, G., van Roozelaar, D.J., Kusters, J.G., Poelwijk, F.A.J., van der Zeijst, B.A.M., 1992. Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. Journal of General Virology 73, 591-596.
Kwon, H.M., Jackwood, M.W., Gelb, J., 1993. Differentiation of Infectious Bronchitis Virus Serotypes Using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis. Avian Diseases 37, 194-202.
Lee, C.W., Hilt, D.A., Jackwood, M.W., 2003. Typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the S1 gene. J Vet Diagn Invest 15, 344-348.
Lin, K.Y., Wang, H.C., Wang, C.H., 2005. Protective effect of vaccination in chicks with local infectious bronchitis viruses against field virus challenge. J Microbiol Immunol Infect 38, 25-30.
Modiri Hamadan, A., Ghalyanchilangeroudi, A., Hashemzadeh, M., Hosseini, H., Karimi, V., Yahyaraeyat, R., et al., 2017. Genotyping of Avian infectious bronchitis viruses in Iran (2015-2017) reveals domination of IS-1494 like virus. Virus Res 240, 101-106.
Montassier, H., 2010. Molecular epidemiology and evolution of avian infectious bronchitis virus. Brazilian Journal of Poultry Science 12, 87-96.
Najafi, H., Langeroudi, A.G., Hashemzadeh, M., Karimi, V., Madadgar, O., Ghafouri, S.A., et al., 2016. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol 161, 53-62.
Seyfi Abad Shapouri, M.R., Mayahi, M., Assasi, K., Charkhkar, S., 2004. A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran. Acta Vet Hung 52, 163-166. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731-2739.
Terregino, C., Toffan, A., Beato, M.S., De Nardi, R., Vascellari, M., Meini, A., et al., 2008. Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes. Avian Pathol 37, 487-493.
Worthington, K.J., Currie, R.J., Jones, R.C., 2008. A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006. Avian Pathol 37, 247-257. | ||
|
آمار تعداد مشاهده مقاله: 2,182 تعداد دریافت فایل اصل مقاله: 3,193 |
||