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zarrabi ahrabi, S., Madani, R., Shemshadi, B., Ranjbar-Bahadori, S., Hashemzadeh Farhang, H., montazer bavili, M. (2019). Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province. , 32(2), 34-41. doi: 10.22092/vj.2018.123937.1519
salar zarrabi ahrabi; Rasool Madani; Bahar Shemshadi; Shahrokh Ranjbar-Bahadori; hossein Hashemzadeh Farhang; majid montazer bavili. "Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province". , 32, 2, 2019, 34-41. doi: 10.22092/vj.2018.123937.1519
zarrabi ahrabi, S., Madani, R., Shemshadi, B., Ranjbar-Bahadori, S., Hashemzadeh Farhang, H., montazer bavili, M. (2019). 'Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province', , 32(2), pp. 34-41. doi: 10.22092/vj.2018.123937.1519
zarrabi ahrabi, S., Madani, R., Shemshadi, B., Ranjbar-Bahadori, S., Hashemzadeh Farhang, H., montazer bavili, M. Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province. , 2019; 32(2): 34-41. doi: 10.22092/vj.2018.123937.1519

Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 5, Volume 32, Issue 2, July 2019, Pages 34-41    PDF (911.93 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2018.123937.1519
Authors
salar zarrabi ahrabi1; Rasool Madani* 2, 3; Bahar Shemshadi1; Shahrokh Ranjbar-Bahadori4; hossein Hashemzadeh Farhang5; majid montazer bavili6
1Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
3Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran
5Department of Parasitology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
6Department of Thoracic Surgery, Faculty of Medical Science, Tabriz Medical University, Tabriz, Iran
Abstract
Hydatidosis has been recognized as one of the most important zoonotic diseases in different parts of the world, including Iran. Serological methods that can detect hydatidosis with high sensitivity and specificity are have high value. In this study, the antigen B fluid of hydatid cysts was isolated and Dot-Blot technique performed on serum samples from human and sheep with hydatid cysts and healthy samples to evaluate their accuracy, sensitivity and specificity. After completing all stages of the dot blot test on 200 samples, it was found that the accuracy of the test in human cases is equivalent to 96% and in sheep cases it is equivalent to 92%. The sensitivity of this test is 92% in human cases and 84% in sheep. The test specificity is also 100% in both cases calculated. The evaluation of all of these cases indicates that B antigens have a high value in the diagnosis in hydatidiosis, and the dot blot technique using this antigen can be valuable as a diagnostic method and also as a method initial screening should be used.
Keywords
Hydatid cyst; Dot blot; Antigen B; Echinococcus granulosus
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