آمار

تعداد نشریات275
تعداد نشریات سازمان74
تعداد شماره‌ها1,689
تعداد مقالات19,572
تعداد مشاهده مقاله10,797,208
تعداد دریافت فایل اصل مقاله8,364,502
Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. (1397). Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ijfs.2019.118495
Saeide Keivanloo; Mohammad Sudagar; Mohammad Mazandarani. "Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C". سامانه مدیریت نشریات علمی, , , 1397, -. doi: 10.22092/ijfs.2019.118495
Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. (1397). 'Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ijfs.2019.118495
Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C. سامانه مدیریت نشریات علمی, 1397; (): -. doi: 10.22092/ijfs.2019.118495

Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C

Iranian Journal of Fisheries Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 04 اسفند 1397  XML اصل مقاله (238.27 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2019.118495
نویسندگان
Saeide Keivanloo1؛ Mohammad Sudagar email 2؛ Mohammad Mazandarani3
1University of Agricultural Science and Natural Res
2د.گرگان
3Gorgan University of Agricultural Sciences & Natur
چکیده
In the present study, cryopreservation of common carp (Cyprinus carpio) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- mL plastic tubes and stored at -2 °C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viability was assessed by survival rate, counting live larvae and number of failed eggs under a stereomicroscope.  The results showed that storage of common carp embryos was possible for up to 7 days at -2 °C, while the survival did not occur when storage period were 14 and 21 days. The combination of methanol with propylene glycol (S2) gave higher survival rate after 1 and 7 days stored at -2 °C. Further studies are needed in order to extend the storage time and to improve the survival rate for this species.
کلیدواژه‌ها
Cryopreservation؛ common carp؛ Cryoprotectant
آمار
تعداد مشاهده مقاله: 6
تعداد دریافت فایل اصل مقاله: 17


Contact Us | Help & Support | Site Map
counter

Journal Management System. Designed by sinaweb.