| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 3,005 |
| تعداد مقالات | 33,638 |
| تعداد مشاهده مقاله | 44,514,038 |
| تعداد دریافت فایل اصل مقاله | 30,307,658 |
تأثیر همزمان شیره خشک گیاه استبرق (Calotropis procera (Willd.) R. Br.) و ویتامین C بر فاکتورهای سرمی موش‌های دیابتی نوع 1 | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 34، شماره 6 - شماره پیاپی 92، بهمن و اسفند 1397، صفحه 986-996 اصل مقاله (264.25 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2019.116279.2189 | ||
| نویسندگان | ||
| فریده ملائی نژاد1؛ فرشته عزتی قادی* 2؛ عبدالله رمضانی قرا2؛ سعید رضایی زارچی3 | ||
| 1دانشجوی کارشناسی ارشد بیوشیمی، دانشگاه پیام نور واحد تفت، یزد، ایران | ||
| 2استادیار، گروه زیست‌شناسی گیاهی، دانشکده علوم پایه، دانشگاه جیرفت، کرمان، ایران | ||
| 3استادیار، گروه زیست، دانشکده علوم پایه، دانشگاه پیام نور واحد تفت، یزد، ایران | ||
| چکیده | ||
| در این مطالعه سعی شد تا به خواص ضد دیابتی شیره خشک گیاه استبرق (Calotropis procera (Willd.) R. Br.) به همراه ویتامین C در موش‌های دیابتی شده توسط STZ پرداخته شود. دیابت در موش‌های بزرگ آزمایشگاهی توسط تک دوز STZ (50 میلی‌گرم/کیلوگرم وزن بدن) به‌صورت تزریق درون صفاقی ایجاد گردید. 30 سر موش نر بالغ نژاد ویستار به‌طور تصادفی به شش گروه به‌شرح زیر تقسیم شدند. گروه اول) کنترل، گروه دوم) دیابتی، گروه سوم) موش‌های دیابتی که با شیره استبرق200 میلی‌گرم به ازای کیلوگرم وزن بدن تیمار شدند، گروه چهارم) گروهی که توسط شیره استبرق (mg/kg200) و ویتامین C (mg/kg100) تیمار شدند، گروه پنجم) تیمار روزانه توسط شیره استبرق (mg/kg200)، گروه ششم) حیوانات این گروه روزانه با ویتامین C (mg/kg200) تیمار شدند. در پایان دوره 15 روزه فاکتورهای بیوشیمیایی قند خون، کلسترول تام، تری‌گلیسیرید و هموگلوبین گلیکه (HbA1c) بررسی شد. نتایج نشان داد که میزان قند خون، کلسترول تام، تری‌گلیسیرید و همگلوبین گلیکه (HbA1c) در گروه دیابتی به‌طور معنی‌داری افزایش داشته است. تیمار گروه‌های دیابتی توسط شیره خشک استبرق و همچنین ترکیب شیره استبرق و ویتامین C باعث کاهش معنی‌دار میزان فاکتورهای بیوشیمیایی بالا گردید. نتایج نشان داد که شیره استبرق به تنهایی نسبت به گروه چهارم تأثیر بهتری بر فاکتورهای بیوشیمیایی سرم داشته است. بنابراین می‌توان نتیجه گرفت که شیره خشک گیاه استبرق به تنهایی قادر به کاهش قند خون و همچنین عوارض ناشی از دیابت در افراد دیابتی نوع 1 می‌باشد. | ||
| کلیدواژه‌ها | ||
| هیپرگلیسمی؛ هیپرلیپیدمی؛ گیاه استبرق (Calotropis procera (Willd.) R. Br.)؛ ویتامین C | ||
| عنوان مقاله [English] | ||
| Simultaneous effects of Calotropis procera (Willd.) R. Br. dried latex and vitamin C on the serum factors of type 1 diabetic rats | ||
| نویسندگان [English] | ||
| F. Molahinejad1؛ F. Ezzati Ghadi2؛ A. Ramezani Ghara2؛ S, Rezaei Zarchi3 | ||
| 1M.Sc. student of Biochemistry, Payam-e-Noor University of Taft, Yazd, Iran | ||
| 2Department of Plant Biology, Faculty of Sciences, University of Jiroft, Jiroft, Iran | ||
| 3Department of Biology, Faculty of Sciences, Payam-e-Noor University of Taft, Yazd, Iran | ||
| چکیده [English] | ||
| The present study investigates the anti-diabetic properties of dried latex of Calotropis procera (Willd.) R. Br.with vitamin C in diabetic rats. Thirty adult male Wistar rats were randomly and equally divided into six groups as follow: first group) control, second group) diabetes was induced by a single dose of streptozotocin (50 mg/kg b.w by IP) , third group) diabetic rats were treated by 200 mg dried latex per kilogram of body weight, fourth group) the diabetic rats were treated by combined treatments of dried latex (200mg/kg) and vitamin C (100mg/kg) by gavage, fifth group) animals in this group were treated by (200mg/kg) dried latex daily and the sixth group) rats were treated by vitamin C (100mg/kg). After 15 days, the biochemical factors of blood sugar, triglyceride, total cholesterol, and glycated hemoglobin (HbAlc) were evaluated. Results showed that the amount of biochemical factors was increased significantly in the diabetic group.Dried latex and also the combined treatment of dried latex and vitamin C reduced the elevated levels of abovementioned biochemical factors. Moreover, the aqueous extract of dried latex compared to the group four, was more effective. In conclusion, the dried latex of Calotropis procera has anti-diabetic activity. | ||
| کلیدواژه‌ها [English] | ||
| Hyperglycemy, hyperlipidemy, Calotropis procera (Willd.) R. Br, vitamin C | ||
| مراجع | ||
|
- Ahmed, M.K., Saleh, M.E., Sayed, M.E. and Shalaby, K.A.F., 2012. Anti-inflammatory effect of different propolis extracts in thioacetamide-induced hepatotoxicity in male rat. Journal of Basic Applied Sciences, 6: 29-40. - Alrheam, A.I.A.A. and Saad-Al Shehri, Z., 2015. Ethanopharmacological study of the aqueous, chloroform, ethanol leaves extracts and latex of Calotropis procera in diabetic rats. Biomedical Research and Therapy, 2(11): 396-401. - Ames, B.N., Shigenaga, M.K. and Hagen, T.M., 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proceeding of the National Academy Sciences, 90(17): 7915-7922. - Amić, D., Davidović-Amić, D., Bešlo, D. and Trinajstić, N., 2003. Structure-radical scavenging activity relationships of flavonoids. Croatica chemica acta, Hrvatsko Kemijsko Društvo, 76(1): 55-61. - Aragno, M., Meineri, G., Vercellinatto, I., Bardini, P., Raimondo, S., Peiretti, P.G., Vercelli, A., Alloatti, G., Tomasinelli, C.E. and Danni, O., 2009. Cardiac impairment in rabbits fed a high-fat diet is counteracted by dehydroepiandrosterone supplementation. Life Science, 85(1): 77-84. - Asmat, U., Abad, K. and Ismail, K., 2016. Diabetes mellitus and oxidative stress-a concise review. Saudi Pharmaceutical Journal, 24(5): 547-553. - Ceriello, A., 2003. New insights on oxidative stress and diabetic complications may lead to a “causal” antioxidant therapy. Diabetes Care, 26(5): - Chen, J., Chen, X., Lei, Y., Wei, H., Xiong, C., Liu, Y., Fu, W. and Ruan, J., 2011. Vascular protective potential of the total flavanol glycosides from Abacopteris penangiana via modulating nuclear transcription factor-κB signaling pathway and oxidative stress. Journal of Ethnopharmacology, 136(1): 217-223. - Dakhale, G.N., Chaudhari, H.V. and Shrivastava, M., 2011. Supplementation of vitamin C reduces blood glucose and improves glycosylated hemoglobin in type 2 diabetes mellitus: a randomized, double-blind study. Advvances in Pharmacological Sciences, 2011: 1-5. - Dimova, R., Tankova, T., Guergueltcheva, V., Tournev, I., Chakarova, N., Grozeva, G. and Dakovska, L., 2017. Risk factors for autonomic and somatic nerve dysfunction in different stages of glucose tolerance. Journal of Diabetes and its Complications, 31(3): 537-543. - Fraga, C.G., Galleano, M., Verstraeten, S.V. and Oteiza, P.I., 2010. Basic biochemical mechanisms behind the health benefits of polyphenols. Molecular Aspects of Medicine, 31(6): 435-445. - Fujiwara, Y., Kondo, T., Murakami, K. and Kawakami, Y., 1989. Decrease of the inhibition of lipid peroxidation by glutathione-dependent system in erythrocytes of non-insulin dependent diabetics. Journal of Molecular Medicine, 67(6): 336-341. - Gajdosik, A., Gajdosikova, A., Stefek, M., Navarova, J. and Hozova, R., 1999. Streptozotocin-induced experimental diabetes in male Wistar rats. General Physiology and Biophysics, 18: 54-62. - Ha, H. and Kim, K.H., 1995. Role of oxidative stress in the development of diabetic nephropathy. Kidney International, 51: S18-21. - Ha, H., Hwang, I.A., Park, J.H. and Lee, H.B., 2008. Role of reactive oxygen species in the pathogenesis of diabetic nephropathy. Diabetes Research and Clinical Practice, 82: S42-S45. - Hallfrisch, J., Singh, V.N., Muller, D.C., Baldwin, H., Bannon, M.E. and Andres, R., 1994. High plasma vitamin C associated with high plasma HDL-and HDL2 cholesterol. The American Journal of Clincal Nutrtrition, 60(1): 100-105. - Heli, H., Amani, M., Moosavi-Movahedi, A.A., Jabbari, A., Floris, G. and Mura, A., 2008. Electroactive centers in Euphorbia latex and lentil seedling amine oxidases. Bioscience Biotechnology and Biochemistry, 72(1): 29-36. - Hillstrom, R.J., Yacapin-Ammons, A.K. and Lynch, S.M., 2003. Vitamin C inhibits lipid oxidation in human HDL. Journal of Nutrition, 133(10): 3047-3051. - Iwata, N., Okazaki, M., Xuan, M., Kamiuchi, S., Matsuzaki, H. and Hibino, Y., 2014. Orally administrated ascorbic acid suppresses neuronal damage and modifies expression of SVCT2 and GLUT1 in the brain of diabetic rats with cerebral ischemia-reperfusion. Nutrition, 6(4): 1554-1577. - Kawakami, Y., Tsurugasaki, W., Nakamura, S. and Osada, K., 2005. Comparison of regulative functions between dietary soy isoflavones aglycone and glucoside on lipid metabolism in rats fed cholesterol. The Journal of Nutritional Biochemistry, 16(4): 205-212. - Kenganora, M., Bhaskaran, M., Santhepete, M.N. and Hukkeri, V.I., 2017. Antioxidant potential of a toxic plant Calotropis procera. Free Radicals and Antioxidants, 7(2): 143-151. - Kumar, G., Karthik, L. and Rao, K.V.B., 2010. Antibacterial activity of aqueous extract of Calotropis gigantea leaves-an in vitro study. International Journal of Pharmaceutical Sciences Review Research, 4(2): 141-144. - Kumar, S., Gupta, A. and Pandey, A.K., 2013. Calotropis procera root extract has the capability to combat free radical mediated damage. Hindawi Publishing Corporation, 2013: 1-8. - Li, C., Miao, X., Li, F., Wang, S., Liu, Q., Wang, Y. and Sun, J., 2017. Oxidative stress-related mechanisms and antioxidant therapy in diabetic retinopathy. Oxidative Medicine and Cellular Longevity, 2017: 1-15. - Lobo, V., Patil, A., Phatak, A. and Chandra, N., 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8): 118-126. - Lorke, D., 1983. A new approach to practical acute toxicity testing. Archives of Toxicology, 54(4): - Lupattelli, G., Marchesi, S., Lombardini, R., Roscini, A.R., Trinca, F., Gemelli, F., Vaudo, G. and Mannarino, E., 2004. Artichoke juice improves endothelial function in hyperlipemia. Life Sciences, 76(7): 775-782. -Nagarajan, S., 2010. Mechanisms of anti-atherosclerotic functions of soy-based diets. The Journal of Nutritional Biochemistry, 21(4): 255-260. - Neto, L., Mário, C., de Vasconcelos, C.F., Thijan, V.N., Caldas, G.F., Araújo, A.V. and Wanderley, A.G., 2013. Evaluation of antihyperglycaemic activity of Calotropis procera leaves extract on streptozotocin-induced diabetes in Wistar rats. Revista Brasileira de Farmacognosia, 23(6): - Omodanisi, E.I., Aboua, Y.G. and Oguntibeju, O.O., 2017. Assessment of the anti-hyperglycaemic, anti-inflammatory and antioxidant activities of the methanol extract of Moringa oleifera in diabetes-induced nephrotoxic male wistar rats. Molecules, 22(439): 1-16. - Padhy, B.M., Srivastava, A. and Kumar, V.L., 2007. Calotropis procera latex affords protection against carbon tetrachloride induced hepatotoxicity in rats. Journal of Ethnopharmacology, 113(3): 498-502. - Rafieian-Kopaei, M., Hosseini, M. and Shirzad, H., 2014. Comment on: effect of pomegranate flower extract on cisplatin-induced nephrotoxicity in rats. Journal of Nephropathology, 3(4): 121-123. - Rahmatullah, M., Sultan, S., Toma, T., Lucky, S., Chowdhury, M., Haque, W., Annay, E. and Jahan, R., 2010. Effect of Cuscuta reflexa stem and Calotropis procera leaf extracts on glucose tolerance in glucose-induced hyperglycemic rats and mice. African Journal of Traditional Complementary and Alternative Medicines, 7(2): 109-112. - Roy, S., Sehgal, R., Padhy, B.M. and Kumar, V.L., 2005. Antioxidant and protective effect of latex of Calotropis procera against alloxan-induced diabetes in rats. Journal of Ethnopharmacology, 102(3): 470-473. - Santos, A. and Van Ree, R., 2011. Profilins: mimickers of allergy or relevant allergens?. International Archives of Allergy and Immunology, 155(3): 191-204. - Sharma, B., Viswanath, G., Salunke, R. and Roy, P., 2008. Effects of flavonoid-rich extract from seeds of Eugenia jambolana (L.) on carbohydrate and lipid metabolism in diabetic mice. Food Chemistry, 110(3): 697-705. - Shen, J., White, M., Husband, A.J., Hambly, B.D. and Bao, S., 2006. Phytoestrogen derivatives differentially inhibit arterial neointimal proliferation in a mouse model. Eurppean Journal of Pharmacology, 548(1): 123-128. - Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T., 1992. Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. Journal of Agricultural and Food Chemistry, 40: 945-948. - Wang, P., Su, Z., Yuan, W., Deng, G. and Li, S., 2012. Phytochemical constituents and pharmacological activities of Eryngium L. (Apiaceae). Pharmaceutical Crops, 3: 99-120. - Wu, J. and Yan, L.J., 2015. Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Diabetes Metabolic Syndromes and Obesity, 8: 181-188. - Zhang, X. and Tan, B.K., 2000. Antihyperglycaemic and anti‐oxidant properties of andrographis paniculata in normal and diabetic rats. Clinical and Experimental Pharmacology and Physiology, 27(5‐6): 358-363. | ||
|
آمار تعداد مشاهده مقاله: 1,230 تعداد دریافت فایل اصل مقاله: 391 |
||