| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,262 |
| تعداد مقالات | 36,057 |
| تعداد مشاهده مقاله | 55,176,190 |
| تعداد دریافت فایل اصل مقاله | 94,175,564 |
شناسایی miRNA های دخیل در مسیر گل دهی گیاه چغندرقند (.Beta vulgaris L) | ||
| چغندرقند | ||
| مقاله 3، دوره 34، شماره 2، بهمن 1397، صفحه 147-163 اصل مقاله (4.3 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/jsb.2019.122102.1199 | ||
| نویسندگان | ||
| میشانه عسگری1؛ اصغر میرزائی اصل* 2؛ محمدرضا عبدالهی3؛ لیلا خدایی4 | ||
| 1دکتری بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه بوعلی سینا. همدان، ایران. | ||
| 2دانشیار گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه بوعلی سینا. همدان، ایران. | ||
| 3دانشیار گروه اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا. همدان، ایران. | ||
| 4استادیار گروه کشاورزی، دانشگاه پیام نور همدان. همدان، ایران. | ||
| چکیده | ||
| یکی از فرآیندهای کنترلی در بسیاری از گیاهان از جمله چغندرقند انتقال از مرحله رشد رویشی به زایشی میباشد که توسط شبکه پیچیدهای از پروتئینها تنظیم میشود. تاکنون چندین مسیر تنظیمی دخیل در مسیر گلدهی و بولتینگ و ژنهای موثر در آنها در گیاه مدل آرابیدوپسیس شناسایی شده است. یک مسیر موثر در انتقال از مرحله رویشی به زایشی مسیر وابسته به سن است که تحتتاثیر دو miRNA172 و miRNA156 میباشد. ژنهای کدکننده miRNA ها در بین گیاهان بسیار گسترده و حفاظتشده هستند. در این تحقیق، برای شناسایی توالی این دو ژن در ژنوم چغندرقند، توالی آنها از چندین گیاه جمعآوری و با توالیهای Refseq-genomic چغندرقند مورد همردیفی قرار گرفتند. نواحی با توالی مشترک شناسایی و اطلاعات آنها به طور دقیق مورد بررسی قرارگرفت. نتایج نشان داد که در ژنوم چغندرقند برای ژن کدکننده miR156 فقط 127 جفت باز از کروموزوم شماره 2 و ژن کدکننده miR172 فقط 110 جفت باز از کروموزوم شماره 9 دارای توالیهای کامل پیشساز miRNA بودند. جداسازی ژن کدکننده miR156 از ژنوم گیاه چغندرقند، با طراحی آغازگرهای اختصاصی از نواحی ابتدا و انتهای ژن انجام گرفت. ژن کدکننده miR156 توسط تکنیک واکنش زنجیرهای پلیمراز و با استفاده از آغازگرهای اختصاصی MIRF و MIRR از روی DNA ژنومی چغندرقند تکثیر شد. همانگونه که انتظار میرفت قطعه DNA 127 جفت باز تکثیر شد و در نهایت از طریق توالییابی مشابهت 99 درصدی توالی تکثیر شده با توالی شناسایی شده ژن کدکننده miR156 به دست آمد و صحت توالی شناسایی شده به عنوان ژن کدکننده miR156 در چغندرقند تأیید گشت. شناسایی و مطالعه این ژنهای مؤثر در فرآیند گلدهی راه را برای قابلیت بررسی عملکرد دقیق آنها در فرآیندهای گلدهی و بولتینگ چغندرقند خواهد گشود. | ||
| کلیدواژه‌ها | ||
| چغندرقند؛ ژن کدکننده؛ مسیر گل دهی؛ miR156؛ miR172 | ||
| عنوان مقاله [English] | ||
| Identification of miRNAs involved in flowering pathway in sugar beet (Beta vulgaris L.) | ||
| نویسندگان [English] | ||
| M. Asgari1؛ A. Mirzaie-asl2؛ M.R. Abdollahi3؛ L. Khodaie4 | ||
| 1Ph.D. in Biotechnology, Agriculture Faculty, Bu-Ali Sina University, Hamedan, Iran. | ||
| 2Associate Professor of Agriculture Faculty, Bu-Ali Sina University, Hamedan, Iran. | ||
| 3Associate Professor of Agriculture Faculty, Bu-Ali Sina University, Hamedan, Iran. | ||
| 4Assistant Professor of Payamenoor University, Hamadan, Iran. | ||
| چکیده [English] | ||
| Transition from vegetative to reproductive stage is one of the important procedures in most crops such as sugar beet which is regulated by the complex network of proteins. So far, several regulatory pathways involved in flowering and bolting and related genes have been identified in the Arabidopsis as a model plant. Age-related pathway is one of the important pathways involved in transition from vegetative to reproductive stage that is influenced by miR156 and miR172. In order to identify the sequence of the above mentioned genes in sugar beet genome, their sequences were collected from several plants and were aligned with sugar beet Refseq-genomic sequence. Regions with a common sequence were identified and evaluated in detail. For miR156 coding gene, only 127 bp in chromosome 2, and for miR172 coding gene only 110 bp in chromosome 9 had the full-length of miR precursor sequence in the sugar beet genome. In order to isolate the miR156 coding gene from the sugar beet genome, specific primers were designed. The miR156 coding gene was amplified from genomic DNA of sugar beet by polymerase chain reaction technique using specific primers of MIRF and MIRR. As expected, the 127 bp gene was amplified and showed 99% similarity with the sequence of the miR156. Identifying and studying these effective genes in the flowering process will open the way for the ability to verify their exact performance in sugar beet flowering and bolting processes. | ||
| کلیدواژه‌ها [English] | ||
| sugar beet, Coding genes, flowering pathway, miR156, miR172 | ||
| مراجع | ||
|
Ambros V. The functions of animal microRNAs. Nature 2004; 431(7006): 350–355. Retrieved from http://dx.doi.org/10.1038/nature02871
Aukerman MJ, Sakai H. Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell 2003; 15(11): 2730–2741.
Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116(2): 281–297.
Bäurle I, Dean C. The timing of developmental transitions in plants. Cell 2006; 125(4): 655–664.
Biancardi E, Campbell LG, Skaracis GN, De Biaggi M. Genetics and breeding of sugar beet. Science Publishers.2005; Retrieved from https://www.crcpress.com/Genetics-and-Breeding-of-Sugar-Beet/Biancardi-Biaggi-Campbell/p/book/9781578083664
Blázquez M, Koornneef M, Putterill J. Flowering on time: Genes that regulate the floral transition: Workshop on the molecular basis of flowering time control. EMBO Reports 2001; 2(12): 1078–1082.
Chia TYP, Müller A, Jung C, Mutasa-Göttgens ES. Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locus. Journal of Experimental Botany 2008; 59(10): 2735–2748.
Deng W, Ying H, Helliwell CA, Taylor JM, Peacock WJ, Dennis ES. Flowering Locus C (FLC) regulates development pathways throughout the life cycle of Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2011; 108(16): 6680–6685.
Dohm JC, Lange C, Holtgräwe D, Sörensen TR, Borchardt D, Schulz B, Lehrach H, Weisshaar B, Himmelbauer H. Palaeohexaploid ancestry for Caryophyllales inferred from extensive gene-based physical and genetic mapping of the sugar beet genome (Beta vulgaris). Plant Journal 2012; 70(3): 528–540.
Han J, Kong ML, Xie H, Sun QP, Nan ZJ, Zhang QZ, Pan JB. Identification of miRs and their targets in wheat (Triticum aestivum L.) by EST analysis. Genetics and Molecular Research 2013; 12(3): 3793–3805.
Hébrard C, Peterson DG, Willems G, Delaunay A, Jesson B, Lefèbvre M, Barnes S, Maury S. Epigenomics and bolting tolerance in sugar beet genotypes. Journal of Experimental Botany 2016; 67(1): 207–225.
Jahanbakhsh-Pour MH, Paknejad F, Habibi D, Aghaeezadeh M, Shahsavan-Baghdadi M. Effect of cycocel on decreasing of bolting in autumn planting of sugar beet in Karaj region. Open Science Journal 2012; 8(2): 137–146.
Jung J-H, Seo Y-H, Seo PJ, Reyes JL, Yun J, Chua N-H, Park C-M. The GIGANTEA-Regulated MicroRNA172 mediates photoperiodic flowering independent of CONSTANS in Arabidopsis. Plant Cell Online 2007; 19(9): 2736–2748.
Kim JJ, Lee JH, Kim W, Jung HS, Huijser P, Ahn JH. The microRNA156-SQUAMOSA promoter binding protein-like3 module regulates ambient temperature-responsive flowering via flowering Locus T in Arabidopsis. Plant Physiology 2012; 159(1): 461–478.
Kim SY, Park BS, Kwon SJ, Kim J, Lim MH, Park YD, Kim DY, Suh SC, Jin YM, Ahn JH, Lee YH. Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant Cell Reports 2007; 26(3): 327–336.
Kobayashi Y, Weigel D. Move on up, it’s time for change - Mobile signals controlling photoperiod-dependent flowering. Genes & Development 2007; 21(19): 2371–2384.
Krol J, Sobczak K, Wilczynska U, Drath M, Jasinska A, Kaczynska D, Krzyzosiak WJ. Structural features of microRNA (miR) precursors and their relevance to miR biogenesis and small interfering RNA/short hairpin RNA design. Journal of Biological Chemistry 2004; 279(40): 42230–42239.
Lau NC, Lim LP, Weinstein EG, Bartel DP. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis Elegans. Science 2001; 294(5543): 858–862.
Li Y, Li W, Jin Y-X. Computational identification of novel family members of microRNA genes in Arabidopsis thaliana and Oryza sativa. Acta Biochimica et Biophysica Sinica 2005; 37(2): 75–87.
Matthias PO, Ulrike B, Sarah D, Rene HP, Marcus L, Claudia M, Detlef B. Overwintering of genetically modfied sugar vulgaris L. subsp. vulgaris, as a source for dispersal of transgenic pollen. Euphytica. 1999; 108(3): 181–186.
Michaels SD. Flowering time regulation produces much fruit. Current Opinion in Plant Biology 2009; 12(1): 75–80.
Mirzaie-Asl A, Asgari M, Alimirzaie M. Evaluation of Flowering Pathways in Arabidopsis thaliana (in Persian). Journal of Crop Biotechnology 2017; 7(18): 57–72. Retrieved from http://ocam.journals.pnu.ac.ir/article_4207_652.html
Mlotshwa S, Yang Z, Kim Y, Chen X. Floral patterning defects induced by Arabidopsis APETALA2 and microRNA172 expression in Nicotiana benthamiana. Plant Molecular Biology 2006; 61(4–5): 781–793.
Murray HG, Thompson WF. Rapid isolation of high molecular weight DNA. Nucleic Acids Research 1980; 8, 4321-4325.
Navarro C, Cruz-Oró E, Prat S. Conserved function of FLOWERING LOCUS T (FT) homologues as signals for storage organ differentiation. Current Opinion in Plant Biology 2015; 23 45–53.
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D. Control of leaf morphogenesis by microRNAs. Nature 2003; 425(6955): 257–263.
Parcy F. Flowering: A time for integration. The International Journal of Developmental Biology 2005; 49(5–6): 585–593.
Pfeiffer N, Tränkner C, Lemnian I, Grosse I, Müller AE, Jung C, Kopisch-Obuch FJ. Genetic analysis of bolting after winter in sugar beet (Beta vulgaris L.). Theoretical and Applied Genetics 2014; 127(11): 2479–2489.
Pin PA, Zhang W, Vogt SH, Dally N, Büttner B, Schulze-Buxloh G, Jelly NS, Chia TY, Mutasa-Göttgens ES, Dohm JC, Himmelbauer H, Weisshaar B, Kraus J, Gielen JJ, Lommel M, Weyens G, Wahl B, Schechert A, Nilsson O, Jung C, Kraft T, Müller AE. The role of a pseudo-response regulator gene in life cycle adaptation and domestication of beet. Current Biology 2012; 22(12): 1095–1101.
Pin P, Life cycle and flowering time control in beet. Dissertation, Department of Forest Genetics and Plant Physiology, Umeå. 2012.
Rana TM. Illuminating the silence: Understanding the structure and function of small RNAs. Nature Reviews Molecular Cell Biology 2007; 8(1): 23–36.
Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP. MicroRNAs in plants. Genes & Development 2002; 16(13): 1616–1626.
Sadeghian SY, Johansson E. Genetic study of bolting and stem length in sugar beet (Beta vulgaris L.) using a factorial cross design. Euphytica 1992; 65(3): 177–185.
Schwarz DS, Zamore PD. Why do miRs live in the miRNP? Genes & Development 2002; 16(9): 1025–1031.
Simpson GG. The autonomous pathway: Epigenetic and post-transcriptional gene regulation in the control of Arabidopsis flowering time. Current Opinion in Plant Biology 2004; 7(5): 570-574.
Sung S, Amasino RM. Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3. Nature 2004; 427(6970): 159–164.
Wang CM, Liu P, Sun F, Li L, Liu P, Ye J, Yue GH. Isolation and identification of miRs in Jatropha curcas. International Journal of Biological Sciences 2012; 8(3): 418–429.
Wang JW, Schwab R, Czech B, Mica E, Weigel D. Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana. Plant Cell Online 2008; 20(5): 1231–1243.
Wang JW, Czech B, Weigel D. MiR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. Cell 2009; 138(4): 738–749.
Xu P, Vernooy SY, Guo M, Hay BA. The drosophila microRNA mir-14 suppresses cell death and is required for normal fat metabolism. Current Biology 2003; 13(9): 790–795.
Yant L, Mathieu J, Dinh TT, Ott F, Lanz C, Wollmann H, Chen X, Schmid M. Orchestration of the floral transition and floral development in Arabidopsis by the bifunctional transcription factor Apetala2. Plant Cell Online 2010; 22(7): 2156–2170.
Zhang B, Pan X, Cobb GP, Anderson TA. Plant microRNA: A small regulatory molecule with big impact. Developmental Biology 2006; 289(1): 3–16.
Zhang BH, Pan XP, Wang QL, Cobb GP, Anderson TA. Identification and characterization of new plant microRNAs using EST analysis. Cell Research 2005; 15(5): 336–360. | ||
|
آمار تعداد مشاهده مقاله: 1,650 تعداد دریافت فایل اصل مقاله: 874 |
||