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ارزیابی تأثیر تغییرات بیوشیمیایی ایجاد شده در مدت هیدروپرایمینگ بر بنیه بذر سه رقم گندم | ||
| علوم و فناوری بذر ایران | ||
| مقاله 8، دوره 7، شماره 2 - شماره پیاپی 14، بهمن 1397، صفحه 105-118 اصل مقاله (1.03 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.110331.1082 | ||
| نویسندگان | ||
| نرگس خمدی* ؛ مجید نبی پور؛ حبیب اله روشنفکر؛ افراسیاب راهنما | ||
| دانشگاه شهید چمران اهواز | ||
| چکیده | ||
| در آزمایش اول، اثر 7 مدت زمان خیساندن بذر (0 ، 6 ، 8 ، 10 ، 12 ، 14 و 16 ساعت ) بر ویژگیهای جوانه زنی و رشد گیاهچه ارقام گندم استار، چمران و فونگ مورد بررسی قرار گرفت. در آزمایش دوم، برخی صفات بیوشیمیایی و فعالیت های آنزیمی در بذرهای پرایم شده در سه مدت زمان 6 ، 8 و 10 ساعت اندازه گیری شد. نتایج آزمایش اول نشان داد که هیدروپرایمینگ با مدت 8 ساعت خیساندن بیشترین اثرات مثبت را بر بنیه بذر ارقام گندم داشت. درمیان ارقام نیز سرعت جوانه زنی در رقم های استار و چمران با یکدیگر اختلاف معنی داری نداشت و کمترین سرعت جوانه زنی در رقم فونگ مشاهده شد. بیشترین طول و وزن خشک ریشه چه در رقم استار مشاهده شد. نتایج آزمایش دوم نشان داد که با افزایش مدت هیدرو پرایمینگ، فعالیت آنزیم α آمیلاز و محتوی قندها و پروتئین های محلول در بذر افزایش یافت. همچنین افزایش محتوی مالون دی آلدئید و فعالیت ناکافی آنزیم های آنتی اکسیدان (افزوده نشدن فعالیت آنزیم با افزایش مدت زمان هیدروپرایمینگ یا کاهش فعالیت آنزیمی) در مدت زمان 10 ساعت هیدروپرایمینگ نسبت به مدت زمان 8 ساعت مشاهده گردید. درمیان ارقام نیز بیشترین و کمترین فعالیت آنزیم α آمیلاز ، محتوی قندها و پروتئین های محلول به ترتیب متعلق به رقم های استار و فونگ بود. در این آزمایش همبستگی فعالیت آنزیم های آنتی اکسیدان با ویژگیهای جوانه زنی و رشد گیاهچه مثبت و معنی دار بود. | ||
| کلیدواژهها | ||
| جوانه زنی؛ رشد گیاهچه؛ مدت هیدروپرایمینگ؛ صفات بیوشیمیایی؛ آنزیمهای آنتی اکسیدان | ||
| عنوان مقاله [English] | ||
| Evaluation seed vigor of wheat as affected by biochemical changes during hydropriming | ||
| نویسندگان [English] | ||
| narges khamadi؛ majid nabipor؛ habibolah roshanfeker؛ afrasyab rahnama | ||
| Shahid Chamran Uivercity | ||
| چکیده [English] | ||
| One of factors in successful seed hydropriming technique is seed imbibition duration. To evaluate hydroprimed seed vigor of three wheat cultivars as affected by seed imbibition durations and also evaluation biochemical changes during hydropriming, two experiments was done as factorial arrangement with completely randomized design. In first experiment, effect of 7 hydropriming durations (0 , 6 , 8 , 10 , 12 , 14 and 16 h ) was research on germination and seedling growth of Star, Chamran and Fong cultivars. In second experiment, some biochemical traits and enzyme activities was measured in primed seeds for 6 , 8 and 10 h hydropriming durations. Results of first experiment showed that hydropriming with 8 h imbibition has the most positive effect on seed vigor of wheat cultivars. Among of cultivars, Star has the higher root length and root weight. Star and Chamran cultivars have no significant difference for germination rate and the lowest of this trait was observed in fong cultivar. Results of second experiment showed that longer hydropriming duration caused greater α amylase enzyme activity and higher contents of soluble carbohydrates and proteins, also increase content of malondialdehyde and insufficient antioxidant enzyme activities in 10 h than 8 h hydropriming duration was observed. Among cultivars the highest and lowest α amylase enzyme activity and soluble carbohydrates and proteins was observed in Star and Fong cultivars respectively. In this research correlation antioxidant enzyme activities with germination and seedling growth was positive and significant. | ||
| کلیدواژهها [English] | ||
| germination, seedling growth, hydopriming duration, biochemical traits, Antioxidant enzymes | ||
| مراجع | ||
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