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Antioxidant and antibacterial activities of C-phycocyanin from common name Spirulina platensis | ||
| Iranian Journal of Fisheries Sciences | ||
| مقاله 123، دوره 19، شماره 4، مهر 2020، صفحه 1911-1927 اصل مقاله (336.12 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2019.118129 | ||
| نویسندگان | ||
| Reza Safari1؛ Zeynab Raftani Amiri* 2؛ Reza Esmaeilzadeh Kenari2 | ||
| 1PhD student | ||
| 2Associate Professor | ||
| چکیده | ||
| In this study, the antibacterial and antioxidant properties of C-phycocyanin (C-PC) from Spirulina platensis were evaluated. The extraction and purification of C-PC were carried out using lyzosyme and ammonium sulphate precipitation, respectively. DPPH radical-scavenging activity, ferric reducing antioxidant power (FRAP)and Fe2+-chelating activity were used for evaluation of antioxidant properties of C-PC. Antibacterial activity was also performed using agar well diffusion and microdilution [minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)]. The selected bacteria were Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Streptococcus iniae and Yersinia ruckeri. The results showed that the biomass production was 1120 mg L-1 and C-PC concentration in crude extracts and purified by (NH4)2SO4 were also 1.815 and 3.75 mg ml-1, respectively. The results of DPPH, FRAP and Fe2+-chelating activitiesof C-PC was 45.75%, 0.051 mg TAE g-1 and 40.23% at zero time and 41.56%, 0.046 mg TAE g-1 and 36.56% after 60 days at -18°C, respectively. The results of agar well diffusion indicated that L. monocytogenes and S. iniae were the most sensitive and resistant, respectively among examined bacteria in different concentrations of C-PC (0- 25 µg ml-1) and the mean of inhibition zones were also 7.50-22.11mm. The results of MIC and MBC of C-PC (as µg ml-1) were 50-500 and 100-500, respectively. As a conclusion, C-PC from S. platensis had high potential of antioxidant activity in vitro and it can be used as natural antioxidants in variety of foods. Antibacterial activity of C-PC was lower than other algal pigments (such as astaxanthin and C-PC in other cyanobacteria). | ||
| کلیدواژهها | ||
| Antibacterial Properties؛ Antioxidant activity؛ C-phycocyanin؛ Spirulina platensis | ||
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