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تعیین غلظت نیمه کشنده (LC50) دو عصاره برگ درخت زیتون (Olea europaea L.) و سیر (Allium sativum L.) در بچه ماهی کپور معمولی (Cyprinus carpio) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 13، دوره 34، شماره 5 - شماره پیاپی 91، آذر و دی 1397، صفحه 849-858 اصل مقاله (186.2 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.122516.2355 | ||
| نویسندگان | ||
| عاطفه کریمی پاشاکی1؛ محدث قاسمی* 2؛ سید جلیل ذریه زهرا3؛ مصطفی شریف روحانی4؛ سید مسعود حسینی5 | ||
| 1دانشجوی دکترای پژوهشمحور بهداشت آبزیان، پژوهشکده آبزیپروری آبهای داخلی، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندر انزلی، ایران | ||
| 2استادیار، پژوهشکده آبزیپروری آبهای داخلی، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندر انزلی، ایران | ||
| 3دانشیار، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4استاد، مؤسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 5استاد، ﮔﺮﻭﻩ ﻣﻴﻜﺮﻭﺑﻴﻮﻟﻮژﻱ و زیستفناوری میکروبی، ﺩﺍﻧﺸﻜﺪﻩ ﻋﻠﻮﻡ و فناوری ﺯﻳﺴﺘﻲ، ﺩﺍﻧﺸﮕﺎﻩ ﺷﻬﻴﺪ ﺑﻬﺸﺘﻲ، ﺗﻬﺮﺍﻥ، ایران | ||
| چکیده | ||
| در این مطالعه، میزان غلظت نیمه کشنده (LC50) عصاره آبی-الکلی برگ درخت زیتون (Olea europaea L.) و عصاره آبی سیر (Allium sativum L.) بر روی بچه ماهی کپور معمولی (Cyprinus carpio) در شرایط آزمایشگاهی بررسی شد. آزمایشها به روش استاندارد O.E.C.D و بهصورت ساکن طی مدت 96 ساعت انجام گردید. میزان ماده مؤثره عصارهها به روش کروماتوگرافی مایع با کارایی بالا (HPLC) اندازهگیری شد. برای اندازهگیری غلظت نیمه کشنده برای هر یک از عصارهها بهطور جداگانه، ماهیها به 5 گروه تیمار و یک گروه شاهد (هریک با 3 تکرار شامل 10 ماهی) تقسیمبندی شدند. میانگین طولی و وزنی ماهیها بهترتیب 0.78±9.8 سانتیمتر و 3.4±15 گرم بود. 5 گروه تیمار در معرض غلظتهای 50، 100، 150، 200 و 250ppm (میلیگرم بر لیتر) از عصاره آبی-الکلی برگ درخت زیتون و 5 گروه تیمار دیگر در معرض غلظتهای 400، 800، 1200، 1600 و2000 ppm از عصاره آبی سیر قرار گرفتند. در طول مدت زمان آزمایش میانگین (SD±) دمای آب 1.7±17 درجه سانتیگراد، اکسیژن محلول 0.41±7.23 میلیگرم در لیتر و pH آب 0.81±7.5 بود. نتایج بدستآمده از کروماتوگرافی مایع با کارایی بالا (HPLC) نشان داد که میزان آلیسین اندازهگیری شده در عصاره سیر mg/g200 و میزان اولئوروپین اندازهگیری شده در برگ زیتون mg/g177.2 بود. مقدار غلظت نیمه کشنده (LC50) 96 ساعته در بچه ماهیان کپور برای عصاره آبی-الکی برگ زیتون ppm168 و برای عصاره آبی سیر ppm1050 بدست آمد. | ||
| کلیدواژهها | ||
| عصاره آبی-الکلی برگ زیتون (Olea europaea L.)؛ عصاره آبی سیر (Allium sativum L.)؛ ماهی کپور معمولی؛ غلظت نیمه کشنده (LC50)؛ کروماتوگرافی HPLC | ||
| عنوان مقاله [English] | ||
| Determination of semi-lethal concentration (LC50) of olive leaf (Olea europaea L.) and garlic (Allium sativum L.) extracts in common carp (Cyprinus carpio) | ||
| نویسندگان [English] | ||
| A. Karimi Pashaki1؛ M. Ghasemi2؛ S.J. Zorriehzahra3؛ M. Sharif Rohani4؛ S.M. Hosseini5 | ||
| 1Ph.D by-Research student in Aquatic Health, Inland Water Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran | ||
| 2Inland Water Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran | ||
| 3Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| 4Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| 5Department of Microbiology & Micorbiotechnology, Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Tehran, Tehran, Iran | ||
| چکیده [English] | ||
| In this study, the semi-lethal concentration (LC50) of aqueous-alcoholic extract of olive (Olea europaea L.) leaf and aqueous extract of garlic (Allium sativum L.) in common carp (Cyprinus carpio) fingerlings was investigated. Experiments were carried out using standard O.E.C.D within 96h. The amount of active ingredient of the extracts was measured by high performance liquid chromatography (HPLC). To measure the semi-lethal concentration for each extract, the fish were divided into five treatment groups and one control group (each with three replicates including 10 fish). Mean length and weight of fish were 9.8±0.78 cm and 15±3.4 g, respectively. Five treatments were exposed to concentrations of 50, 100, 150, 200 and 250 ppm (mg/l) of aqueous-alcoholic extract of olive leaf and five other treatments were exposed to concentrations of 400, 800, 1200, 1600 and 2000 ppm of aqueous extract of garlic. During the test period, the average (±SD) temperature was 17±1.7 °C, the dissolved oxygen was 7.23±0.41 mg/l and the pH was 7.5±0.81. The results of high performance liquid chromatography (HPLC) showed that the amount of Allicin measured in garlic extract was 200 mg/g and the amount of Oleoropein measured in olive leaf was 177.2 mg/g. The 96h LC50 value in common carp for the aqueous-alcoholic extract of olive leaf was calculated to be 168 ppm and for the aqueous extract of garlic, 1050 ppm. | ||
| کلیدواژهها [English] | ||
| aqueous-alcoholic extract of olive leaf (Olea europaea L.), aqueous extract of garlic (Allium sativum L.), common carp, semi-lethal concentration (LC50), HPLC | ||
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