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بررسی اثربخشی ترکیب ژل صبرزرد (Aloe vera L.) با عصاره هیدرواتانولی پوست میوه پسته کوهی (Pistacia atlantica Desf.) بر روند التیام زخم برشی- برداشتی در موش آزمایشگاهی دیابتی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 9، دوره 34، شماره 5 - شماره پیاپی 91، آذر و دی 1397، صفحه 794-804 اصل مقاله (405.56 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.121246.2286 | ||
| نویسندگان | ||
| سیاوش ابراهیمی1؛ محمدرضا فرهپور* 2؛ محمد صائب نجار1 | ||
| 1دانشآموخته دامپزشکی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران | ||
| 2دانشیار، گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران | ||
| چکیده | ||
| تأخیر در روند التیام زخم، یکی از عوارض دیابت است. بنابراین، از دیدگاه درمانی، تلاش برای پیدا کردن ترکیبهای مؤثر در افزایش سرعت روند التیام زخم، بسیار مهم است. این مطالعه بهمنظور بررسی اثرهای ترکیب ژل گیاه صبرزرد (Aloe vera L.) و عصاره هیدرواتانولی پوست میوه پسته کوهی (Pistacia atlantica Desf.) بر روند التیام زخم در 54 سر موش مبتلا به دیابت نوع 2 تجربی انجام گردید. یک زخم تمام ضخامت برشی توسط پانچ بیوپسی 6 میلیمتری بر پوست ناحیه پشت هر موش ایجاد شد. همه موشها به سه گروه دارونما، ژل صبرزرد 5% (w/w) و ترکیب ژل صبرزرد 5% با عصاره هیدرواتانولی پوست میوه پسته کوهی 5% (w/w) تقسیم شدند. سرعت بسته شدن زخم در روزهای 4، 8، 12 و 16 اندازهگیری شد. بهلحاظ آسیب شناختی، روند ترمیم زخم از نظر میزان ادم، نفوذ سلولی، نوزایش عروقی و رسوب کلاژن بین گروهها مقایسه گردید. میزان انقباض زخم در گروههای درمانی نسبت به گروه کنترل افزایش معنیداری (0.05>P) را نشان داد. میزان ادم در هر دو گروه درمانی نسبت به گروه کنترل کاهش یافت، در حالی که نوزایش عروقی، نفوذ فیبروبلاستها و تولید کلاژن افزایش نشان داد؛ بهطوری که این افزایش در گروه درمانی با پماد ترکیبی از میزان بالاتری برخوردار بود. نتایج این مطالعه نشان داد که تجویز موضعی ترکیب ژل صبرزرد 5% با عصاره هیدرواتانولی پوست میوه پسته کوهی 5% التیام زخم تمام ضخامت برشی پوست را در موشهای دیابتی نوع 2 با کاهش میزان التهاب بافتی و افزایش تولید کلاژن و نوسازی بافت پوششی بهبود میبخشد و میتواند بهعنوان یک داروی جدید مورد استفاده قرار گیرد. | ||
| کلیدواژهها | ||
| ژل صبر زرد (Aloe vera L.)؛ پسته کوهی (Pistacia atlantica Desf.)؛ عصاره هیدرواتانولی؛ زخم پوست؛ دیابت؛ موش | ||
| عنوان مقاله [English] | ||
| Efficiency of co-administration of Aloe vera L. gel and hydroethanolic extract of Pistacia atlantica Desf. on the wound healing process of excisional wound in diabetic mice | ||
| نویسندگان [English] | ||
| S. Ebrahimi1؛ M.R. Farahpour2؛ M. Saebnajjar1 | ||
| 1Graduated Veterinary Medicine, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran | ||
| چکیده [English] | ||
| One of the side effects of diabetes is delay in wound healing process. Thus, the use of effective compounds for fasten the wound healing process is important. The present study was conducted to investigate the combined effects of Aloe vera L. gel and hydroethanolic extract of Pistacia atlantica Desf. hulls on wound healing process in 54 adult mice with type-2 diabetes. A circle full-thickness excisional wound model was created on the back of each mouse by a 6-mm punch biopsy. The mice were divided into three groups including control group, 5% Aloe vera gel (w/w) and a combined of 5% Aloe vera gel + 5% Pistacia atlantica extract (w/w). Wound closure rate was evaluated at 4, 8, 12 and 16 days. Edema, cellular infiltration, revascularization, and collagen deposition were assessed among groups. Wound contraction ratio was significantly higher in the treated groups in comparison to control group (P<0.05). The edema was significantly decreased in the treated groups compared with control group, while fibroblast infiltration, revascularization and collagen deposition were significantly increased in same groups in comparison to control group (P<0.05). The best response was observed in the combined group. Results showed that topical administration of 5% Aloe vera gel + 5% Pistacia atlantica extract, in combined form, accelerated full-thickness excisional wound healing in type 2 diabetic mice by decreasing tissue inflammation and increasing collagen synthesis and re-epithelization and it can be considered as a new drug. | ||
| کلیدواژهها [English] | ||
| Aloe vera L. gel, Pistacia atlantica Desf. hull, hydroethanolic extract, cutaneous wound, diabetes, rat | ||
| مراجع | ||
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