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The synergistic influence of the sea cucumber (Holothuria arenicola) extract and dacarbazine on murine melanoma cancer cells B16F10 | ||
| Iranian Journal of Fisheries Sciences | ||
| مقاله 13، دوره 18، شماره 1، فروردین 2019، صفحه 173-187 اصل مقاله (570.67 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2018.118806. | ||
| نویسندگان | ||
| Javad Baharara* 1؛ Najme Nikdel2؛ Khadije Nezhad Shahrokhabadi3؛ Elaheh Amini4 | ||
| 1Department of Development Biology, Research Center for Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. | ||
| 2Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. | ||
| 3Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran | ||
| 4Young Researchers and Elicit club, Mashhad Branch, Islamic Azad University, Mashhad, Iran. | ||
| چکیده | ||
| Melanoma has been reported a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) have been examined on melanoma cells and compared with dacarbazine. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of methanolic SCE Also, several methods were exerted to detect cell dying by SCE and dacarbazine. The MTT assay showed that B16F10 cells proliferation were blocked by SCE (IC50=31µg/ml) and dacarbazine (IC50=1600 µg/ml) in a dose and time depended manner. Apoptosis induction yield treatment occurred in IC50 concentration of SCE and dacarbazine using DAPI staining, acridine orange/ propodium iodide, PI flow cytometry and annexin/PI assay. The caspase colorimetric kit indicated that SCE and dacarbazine could induce apoptosis through intrinsic pathway. The results suggested that the methanolic SCE has higher efficient cytotoxicity efficiency compared to dacarbazine. Therefore, SCE may be considered as a futuristic natural product regarding prevention or treatment of melanoma malignancy. | ||
| کلیدواژهها | ||
| Apoptosis؛ natural product؛ melanoma؛ Cancer؛ dacarbazine؛ marine؛ caspase | ||
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