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Evaluation of Pertussis Toxin Expression in B2 and THIJS Media | ||
| Archives of Razi Institute | ||
| مقاله 7، دوره 74، شماره 1، خرداد 2019، صفحه 59-67 اصل مقاله (366.42 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.110249.1124 | ||
| نویسندگان | ||
| H. Moradtalab1؛ M. Noofeli* 2؛ H. Zeighami1؛ F. Haghi1 | ||
| 1Department of Microbiology and Virology, Faculty of Medical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran | ||
| 2Department of Human Bacterial Vaccines Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran | ||
| چکیده | ||
| Whole-cell pertussis vaccine (wP) has been imperative and highly effective in preventing childhood deaths due to pertussis. Pertussis toxin is one of the virulence factors of Bordetella pertussis in all available pertussis vaccines. wP production in Razi Vaccine and Serum Research Institute is according to bioreactor culture of B. pertussis strains in B2 medium. The aim of this study was to evaluate B. pertussis strain 509 PT production in B2 and Thalen-IJssel (THIJS) media by Chinese Hamster Ovary (CHO) cell and enzyme-linked immunosorbent assay methods (ELISA). In the current study, B. pertussis strain 509 was cultured in B2 and THIJS media. Six samples were taken during the log growth phase within 2-3 h intervals (triplicate). The growth rate was calculated using opacity and the quantification of cell-associated and released PT measured by ELISA and CHO cell assays. THIJS medium was significantly showed an increase in the bacterial growth rate. During the first 29 h, bacterial concentrations in B2 and THIJS culture medium were 19 and 29 IOU, respectively. In THIJS medium, greater amount of pertussistoxin production was cell-associated. In B2 medium, maximum cell-associated toxin by ELISA and CHO cell assays were in the ODs of 1.1 and 0.9 and for THIJS medium in the ODs of 1.6 and 1.1, respectively. B. pertussis strain 509 in THIJS medium produced higher cell mass and cell-associated pertussis toxin than that of B2. It can be used for the production of whole-cell vaccine with higher pertussis toxin and accordingly using lower biomass per dose leading to the reduction of vaccine toxicity. | ||
| کلیدواژهها | ||
| Bordetella pertussis؛ Pertussis Vaccine؛ ELISA؛ CHO cells؛ Pertussis Toxin | ||
| مراجع | ||
|
2014. Meeting of the Strategic Advisory Group of Experts on immunization, April 2014 -- conclusions and recommendations. Wkly Epidemiol Rec 89, 221-236.
Black, R., Cousens, S., Johnson, H., Lawn, J., Rudan, I., Bassani, D., et al., 2010. Presentation for the Child Health Epidemiology Reference Group of WHO and UNICEF. Global, regional, and national causes of child mortality in 2008: A systematic analysis. Lancet 375, 1969-1987.
Bogdan, J.A., Nazario-Larrieu, J., Sarwar, J., Alexander, P., Blake, M., 2001. Bordetella pertussis autoregulates pertussis toxin production through the metabolism of cysteine. Infect Immun 69, 6823-6830.
Coutte, L., Locht, C., 2015. Investigating pertussis toxin and its impact on vaccination. Future Microbiol. 10, 241-254.
Craig-Mylius, K.A., Weiss, A.A., 2000. Antibacterial agents and release of periplasmic pertussis toxin from Bordetella pertussis. Am Soc Microbiol, pp. 1383-1386.
Crowcroft, N., Stein, C., Duclos, P., Birmingham, M., 2003. How best to estimate the global burden of pertussis? Lancet Infect Dis 3, 413-418.
Edwards, K.M., 2005. Overview of pertussis: focus on epidemiology, sources of infection, and long term protection after infant vaccination. Pediatr Infect Dis J 24, S104-S108.
Fine, P.E., Clarkson, J.A., 1987. Reflections on the efficacy of pertussis vaccines. Rev Infect Dis 9, 866-883.
Gillenius, P., Jäätmaa, E., Askelöf, P., Granström, M., Tiru, M., 1985. The standardization of an assay for pertussis toxin and antitoxin in microplate culture of Chinese hamster ovary cells. J Biol Stand 13, 61-66.
Halperin, S.A., Bortolussi, R., Kasina, A., Wort, A.J., 1990. Use of a Chinese hamster ovary cell cytotoxicity assay for the rapid diagnosis of pertussis. J Clin Microbiol 28, 32-38.
Liu, L., Oza, S., Hogan, D., Perin, J., Rudan, I., Lawn, J.E., et al., 2015. Global, regional, and national causes of child mortality in 2000–13, with projections to inform post-2015 priorities: an updated systematic analysis. Lancet 385, 430-440.
Steinhoff, M.C., Reed, G.F., Decker, M.D., Edwards, K.M., Englund, J.A., Pichichero, M.E., et al., 1995. A randomized comparison of reactogenicity and immunogenicity of two whole-cell pertussis vaccines. Pediatrics 96, 567-570.
Streefland, M., van de Waterbeemd, B., Happe, H., van der Pol, L.A., Beuvery, E.C., Tramper, J., et al., 2007. PAT for vaccines: the first stage of PAT implementation for development of a well-defined whole-cell vaccine against whooping cough disease. Vaccine 25, 2994-3000.
Streefland, M., van de Waterbeemd, B., Kint, J., van der Pol, L.A., Beuvery, E.C., Tramper, J., et al., 2009a. Evaluation of a critical process parameter: oxygen limitation during cultivation has a fully reversible effect on gene expression of Bordetella pertussis. Biotechnol Bioengin 102, 161-167.
Streefland, M., Van Herpen, P., Van de Waterbeemd, B., Van der Pol, L., Beuvery, E., Tramper, J., et al., 2009b. A practical approach for exploration and modeling of the design space of a bacterial vaccine cultivation process. Biotechnol Bioengin 104, 492-504.
Thalen, M., van den IJssel, J., Jiskoot, W., Zomer, B., Roholl, P., de Gooijer, C., et al., 1999. Rational medium design for Bordetella pertussis: basic metabolism. J Biotechnol. 75, 147-159.
Thalen, M., Venema, M., Dekker, A., Berwald, L., van den IJssel, J., Zomer, B., et al., 2006a. Fed-batch cultivation of Bordetella pertussis: Metabolism and Pertussis Toxin production. Biologicals 34, 289-297.
Thalen, M., Venema, M., van den IJssel, J., Berwald, L., Beuvery, C., Martens, D., et al., 2006b. Effect of relevant culture parameters on Pertussis Toxin expression by Bordetella pertussis. Biologicals 34, 213-220.
Van de Waterbeemd, B., Streefland, M., Pennings, J., van der Pol, L., Beuvery, C., Tramper, J., et al., 2009. Gene expression based quality scores indicate optimal harvest point in Bordetella pertussis cultivation for vaccine production. Biotechnol Bioengin 103, 900-908.
Verwey, W., Thiele, E.H., Sage, D.N., Schuchardt, L.F., 1949. A simplified liquid culture medium for the growth of Hemophilus pertussis. J Bacteriol 58, 127.
Westdijk, J., van den Ijssel, J., Thalen, M., Beuvery, C., Jiskoot, W., 1997. Quantification of cell-associated and free antigens in Bordetella pertussis suspensions by antigen binding ELISA. J Immunoassay Immunochem. 18, 267-284.
Witt, M.A., Arias, L., Katz, P.H., Truong, E.T., Witt, D.J., 2013. Reduced risk of pertussis among persons ever vaccinated with whole cell pertussis vaccine compared to recipients of acellular pertussis vaccines in a large US cohort. Clin Infect Dis 56, 1248-1254. World Health, O., 2013. Manual for quality control of diphtheria, tetanus and pertussis vaccines. In: Organization, W.H. (Ed.), Immunization, Vaccines and Biologicals. World Health Organization, Switzerland, pp. 192-197. | ||
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