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ارزیابی الگوی بیان ژنهای NAC2، MYB و CBF14 ژنوتیپهای حساس و مقاوم به شوری در آژیلوپس(Aegilops) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 9، دوره 26، شماره 2 - شماره پیاپی 52، دی 1397، صفحه 244-253 اصل مقاله (314.8 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.117963 | ||
| نویسندگان | ||
| علی محمودی1؛ علی اعلمی* 2؛ حسن حسنی کومله3؛ مسعود اصفهانی4؛ رضا شیرزادیان3 | ||
| 1دانش آموخته کارشناسی ارشد بیوتکنولوژی، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
| 2نویسنده مسئول مکاتبات، دانشیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت پست الکترونیک:ali_aalami@guilan.ac.ir | ||
| 3استادیار، گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
| 4استاد، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1397.2.244.52.26.1575.41 تنش شوری یکی از اصلیترین عوامل محیطی است که تولید و توزیع جغرافیایی گیاهان را در سراسر جهان محدود میکند. آژیلوپس یک جنس وحشی از خانواده گندمیان است که دارای ژنهای مفید همانند تحمل به تنشهای محیطی میباشد. در این آزمایش ژنوتیپ 575 از گونه Aegilops cylindrica بهعنوان مقاوم و ژنوتیپ 675 از گونه Aegilops crassa بهعنوان حساس که بر پایه آزمایشهای پیشین در تنش شوری شناسایی شده بودند، استفاده شد. پس از جوانهزنی بذرها، گیاهچهها در شرایط کنترل شده نوری و دمایی بهمحیط ماسه منتقل و آبیاری در دو شرایط نرمال و تنش شوری 200 میلی مولار با محلول هوگلند انجام گردید. نمونهبرداری از بافت برگ در زمانهای صفر، شش، 24، 48 و 72 ساعت پس از تیمار شوری انجام شد. پس از استخراج RNA و ساخت cDNA صحت آن با 18S rRNA تأیید و بیان ژنهای سه عوامل رونویسی CBF14، NAC2 و MYB از طریق Real Time-PCR بررسی شد. نتایج نشان داد که فاکتورهای رونویسی NAC2 و CBF14 48 ساعت پس از اعمال تنش شوری بالاترین میزان بیان را داشتند، ولی ژن MYB بیشترین بیان را 24 ساعت پس از اعمال تنش داشت. در مجموع بیان ژنهای CBF14، NAC2 و MYBدر ژنوتیپ متحمل 575 در مقایسه با ژنوتیپ 675 در تمامی ساعات بیان نسبی بیشتری داشت. این نتایج نشان میدهد که سه ژن فوق نقش کلیدی و مهمی در اعطای تحمل به تنش شوری در ژنوتیپ متحمل دارند، بنابراین با توجه بهقرابت ژنتیکی بالای آژیلوپس و گندم میتوانند پس از آزمایشهای تکمیلی بهعنوان ژنهای کاندیدا برای استفاده در برنامههای بهنژادی گندم بهکار روند. | ||
| کلیدواژهها | ||
| آژیلوپس؛ تنش شوری؛ فاکتورهای رونویسی | ||
| عنوان مقاله [English] | ||
| Assessment of NAC2, MYB and CBF14 genes expression in susceptible and resistant Aegilops genotypes to salinity | ||
| نویسندگان [English] | ||
| A. Mahmoudi1؛ A. Aalami2؛ H. Hasani Komleh3؛ M. Esfehani4؛ R. Shirzadian3 | ||
| 1Former M.Sc. student of Biotechnology, Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran | ||
| 2Corresponding author, Assoc. Prof., Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran. Email: ali_aalami@guilan.ac.ir | ||
| 3Assist. Prof., Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran. | ||
| 4Prof., Dep. of Agronomy and plant breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1397.2.244.52.26.1575.41 Salinity is one of the main environmental factors limiting production and distribution of plants worldwide. Aegilops is a wild species from grasses family with useful genes such as environmental stress tolerance. Two genotypes, 575 from Aegilop cylindrica as resistant and 675 from Aegilop crassa as sensitive to salinity were used base on previous experiments in salinity stress. After seed germination, the seedlings were transferred to a sandy environment under controlled light and temperature conditions and irrigation was performed under two normal conditions and 200 mM salinity with Hoagland solution. Leaf tissue samples were taken at 0, 6, 24, 48 and 72 hours after salinity treatments. After extracting the RNA and the cDNA synthesis, its accuracy was verified by 18S rRNA. The expression of the three transcription factors of CBF14, NAC2 and MYB was investigated by using Real Time-PCR. Results showed that NAC2 and CBF14 transcription factors had the highest expression at 48 hours after salinity stress, but the MYB gene had the highest expression at 24 hours after stress. Overall, the expression of CBF14, NAC2, and MYB genes in the 575 genotype compared with genotype 675 was in higher level in all hours. The results showed that the three mentioned genes play important role in salt tolerance in the tolerant genotype, therefore, considering the high genetic similarity between Aegilops and wheat, the genes can be as candidate genes for using in wheat breeding programs. | ||
| کلیدواژهها [English] | ||
| Aegilops, Real-time PCR, Salinity stress, Transcription factors | ||
| مراجع | ||
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