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کارایی نشانگر مولکولی SCoT در بررسی تمایز دو گونه Lolium perenne و Lolium multiflorum | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 6، دوره 26، شماره 2 - شماره پیاپی 52، دی 1397، صفحه 207-220 اصل مقاله (310.05 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.117960 | ||
| نویسندگان | ||
| محسن فرشادفر* 1؛ هومن شیروانی2؛ مصطفی امجدیان3؛ آنیتا یاقوتی پور4 | ||
| 1مسئول مکاتبات، دانشیار، گروه کشاورزی، دانشگاه پیام نور، پست الکترونیکی: farshadfarmohsen@yahoo.com | ||
| 2مدرس گروه کشاورزی، دانشگاه پیام نور | ||
| 3مربی، گروه کشاورزی، دانشگاه پیام نور | ||
| 4دکترای اصلاح نباتات، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه رازی، کرمانشاه | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109 چچم یکی از گیاهان مهم علوفهای در مناطق سردسیری جهان میباشد و در شرایط مختلف آب و هوایی رشد میکند. تنوع ژنتیکی دو گونه بین نه ژنوتیپ از Lolium perenne و نه ژنوتیپ از Lolium multiflorum با استفاده از 15 نشانگر مولکولی SCoT بررسی شد. آغازگرهای ScoT در مجموع 86 باند تولید کردند که از این تعداد 12 باند یکشکل مشاهده شد و سایر باندها چندشکل بودند. آغازگرهای SC35، SC36 و SC26 بهترتیب با 9 و 8 باند بیشترین تعداد باند و آغازگرهای SC10 و SC44 با 2 و 3 باند کمترین تعداد باند را نشان دادند. بیشترین میزان محتوای اطلاعات چندشکلی (PIC) مربوط به آغازگرهای SC5، SC40، SC35، SC63، SC13، SC44، SC26، SC21 و SC10 بود. بهطوریکه بیشترین میزان شاخص قدرت تفکیک (RP)، شاخص نشانگری (MI) و نسبت چندگانه مؤثر (EMR) را آغازگرهای SC35 و SC26 داشتند. تشابه ژنتیکی ژنوتیپهای مورد بررسی با استفاده از ضریب تشابه جاکارد از 35/0 تا 91/0 متغیر بود و میانگین تشابه بین ژنوتیپها برابر 64/0 بود. بر اساس تجزیه خوشهای ژنوتیپها در 2 گروه قرار گرفتند، بهطوریکه گونهها بهصورت صد در صد از یکدیگر تفکیک شدند. این نتایج با نتایج حاصل از تجزیه به مختصات اصلی (PCoA) تأیید شد. تجزیه واریانس مولکولی (AMOVA) بر اساس گونههای یکساله و چند ساله نشان داد که تنوع در درون گونهها برابر 51% و در بین گونهها برابر 49% بود. بر اساس شاخصهای هتروژنتیکی مورد انتظار (He) و شاخص شانون (I) بیشترین تنوع درون گونهای و تنوع ژنی مربوط به گونه L. Multiflorum بود. | ||
| کلیدواژهها | ||
| تنوع ژنتیکی؛ چچم؛ نشانگر مولکولی | ||
| عنوان مقاله [English] | ||
| Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species | ||
| نویسندگان [English] | ||
| M. Farshadfar1؛ H. Shirvani2؛ M. Amjadian3؛ A. Yaghotipoor4 | ||
| 1Corresponding author, Assoc. Prof., Department of Agriculture, Payame Noor University, I.R. Iran Email: farshadfarmohsen@yahoo.com | ||
| 2Instructor, Department of Agriculture, Payame Noor University, I.R. Iran | ||
| 3Department of Agriculture, Payame Noor University, I.R. Iran | ||
| 4Ph.D in Plant breeding, Department of Agronomy and Plant breeding, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, I.R. Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109 Lolium is an important cool season grass species growing in different climates. To identify variation among nine Lolium multiflorum and nine Lolium perenne genotypes, this experiment was carried out based on 15 SCoT molecular markers. All of the SCoT primers showed 86 visible bands. Seventy four out of 86 bands were polymorphs. Primers SC35, SC36 and SC26 (with 9 and 8 bands respectively) showed the highest number of bands and SC10 and SC44 (with 2 and 3 bands) showed the lowest number of bands. The greatest number of band for polymorphic information content (PIC) belonged to SC5، SC40، SC35 ،SC63 ،SC13, C44، SC26 ،SC21and SC10. The greatest Resolving Power (RP), Marker Index (MI), Effective Multiplex Ratio (EMR) belonged to SC35 andSC26 primers. Genetic similarity based on Jaccard coefficient ranged from 0.35 to 0.91. Mean similarity between the genotypes was 0.64. Cluster analysis grouped genotypes into two groups. The results of clustering was confirmed by principal coordinate (PCo) analysis. Analysis of molecular variance of annual and perennial species revealed that variation within species was 51% and variation between species was 49%. Based on heterogeneity (He) and Shannon indices (I), the highest intraspecific variability and gene variation belonged to L. Multiflorum. | ||
| کلیدواژهها [English] | ||
| Genetic variability, Lolium multiflorum, Lolium perenne, molecular marker, SCoT | ||
| مراجع | ||
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