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کارایی نشانگر مولکولی SCoT در بررسی تمایز دو گونه Lolium perenne و Lolium multiflorum | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 6، دوره 26، شماره 2 - شماره پیاپی 52، دی 1397، صفحه 207-220 اصل مقاله (310.05 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.117960 | ||
| نویسندگان | ||
| محسن فرشادفر* 1؛ هومن شیروانی2؛ مصطفی امجدیان3؛ آنیتا یاقوتی پور4 | ||
| 1مسئول مکاتبات، دانشیار، گروه کشاورزی، دانشگاه پیام نور، پست الکترونیکی: farshadfarmohsen@yahoo.com | ||
| 2مدرس گروه کشاورزی، دانشگاه پیام نور | ||
| 3مربی، گروه کشاورزی، دانشگاه پیام نور | ||
| 4دکترای اصلاح نباتات، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه رازی، کرمانشاه | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109 چچم یکی از گیاهان مهم علوفهای در مناطق سردسیری جهان میباشد و در شرایط مختلف آب و هوایی رشد میکند. تنوع ژنتیکی دو گونه بین نه ژنوتیپ از Lolium perenne و نه ژنوتیپ از Lolium multiflorum با استفاده از 15 نشانگر مولکولی SCoT بررسی شد. آغازگرهای ScoT در مجموع 86 باند تولید کردند که از این تعداد 12 باند یکشکل مشاهده شد و سایر باندها چندشکل بودند. آغازگرهای SC35، SC36 و SC26 بهترتیب با 9 و 8 باند بیشترین تعداد باند و آغازگرهای SC10 و SC44 با 2 و 3 باند کمترین تعداد باند را نشان دادند. بیشترین میزان محتوای اطلاعات چندشکلی (PIC) مربوط به آغازگرهای SC5، SC40، SC35، SC63، SC13، SC44، SC26، SC21 و SC10 بود. بهطوریکه بیشترین میزان شاخص قدرت تفکیک (RP)، شاخص نشانگری (MI) و نسبت چندگانه مؤثر (EMR) را آغازگرهای SC35 و SC26 داشتند. تشابه ژنتیکی ژنوتیپهای مورد بررسی با استفاده از ضریب تشابه جاکارد از 35/0 تا 91/0 متغیر بود و میانگین تشابه بین ژنوتیپها برابر 64/0 بود. بر اساس تجزیه خوشهای ژنوتیپها در 2 گروه قرار گرفتند، بهطوریکه گونهها بهصورت صد در صد از یکدیگر تفکیک شدند. این نتایج با نتایج حاصل از تجزیه به مختصات اصلی (PCoA) تأیید شد. تجزیه واریانس مولکولی (AMOVA) بر اساس گونههای یکساله و چند ساله نشان داد که تنوع در درون گونهها برابر 51% و در بین گونهها برابر 49% بود. بر اساس شاخصهای هتروژنتیکی مورد انتظار (He) و شاخص شانون (I) بیشترین تنوع درون گونهای و تنوع ژنی مربوط به گونه L. Multiflorum بود. | ||
| کلیدواژهها | ||
| تنوع ژنتیکی؛ چچم؛ نشانگر مولکولی | ||
| عنوان مقاله [English] | ||
| Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species | ||
| نویسندگان [English] | ||
| M. Farshadfar1؛ H. Shirvani2؛ M. Amjadian3؛ A. Yaghotipoor4 | ||
| 1Corresponding author, Assoc. Prof., Department of Agriculture, Payame Noor University, I.R. Iran Email: farshadfarmohsen@yahoo.com | ||
| 2Instructor, Department of Agriculture, Payame Noor University, I.R. Iran | ||
| 3Department of Agriculture, Payame Noor University, I.R. Iran | ||
| 4Ph.D in Plant breeding, Department of Agronomy and Plant breeding, Faculty of Science and Agriculture Engineering, Razi University, Kermanshah, I.R. Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109 Lolium is an important cool season grass species growing in different climates. To identify variation among nine Lolium multiflorum and nine Lolium perenne genotypes, this experiment was carried out based on 15 SCoT molecular markers. All of the SCoT primers showed 86 visible bands. Seventy four out of 86 bands were polymorphs. Primers SC35, SC36 and SC26 (with 9 and 8 bands respectively) showed the highest number of bands and SC10 and SC44 (with 2 and 3 bands) showed the lowest number of bands. The greatest number of band for polymorphic information content (PIC) belonged to SC5، SC40، SC35 ،SC63 ،SC13, C44، SC26 ،SC21and SC10. The greatest Resolving Power (RP), Marker Index (MI), Effective Multiplex Ratio (EMR) belonged to SC35 andSC26 primers. Genetic similarity based on Jaccard coefficient ranged from 0.35 to 0.91. Mean similarity between the genotypes was 0.64. Cluster analysis grouped genotypes into two groups. The results of clustering was confirmed by principal coordinate (PCo) analysis. Analysis of molecular variance of annual and perennial species revealed that variation within species was 51% and variation between species was 49%. Based on heterogeneity (He) and Shannon indices (I), the highest intraspecific variability and gene variation belonged to L. Multiflorum. | ||
| کلیدواژهها [English] | ||
| Genetic variability, Lolium multiflorum, Lolium perenne, molecular marker, SCoT | ||
| مراجع | ||
|
- Abbaszade, S., Jafari, AA. and Safari, H., 2013a. Evaluation of genetic diversity of the seven Lolium Multiflorum diplopid population using the ISSR marker, The 8th Biotechnology Conference of the Islamic Republic of Iran and the 4th National Bioethics Conference, Iran. - Abbaszade, S., Jafari, A.A. and Safari, H., 2013b. Evaluation of Genetic Diversity of the Seven Lolium Multiflorum Tetraplopid Population Using the ISSR Marker, The 8th Biotechnology Conference of the Islamic Republic of Iran and the 4th National Bioethics Conference. - Altıntas, S., Toklu, F., Kafkas, S., Kilian, B., Brandolini, A. and Zkan, H. O., 2008. Estimating genetic diversity in durum and bread wheat cultivars from Turkey using AFLP and SAMPL markers. Plant Breeding, 127: 9-14. - Bolaric, S., Barth, S., Melchinger, A.E. and Posselt, U.K., 2005. Genetic diversity in European perennial ryegrass cultivar investigated with RAPD markers. Plant Boching, 124: 161-166. - Buckler, E.S., and Thornsherry, J.M., 2002. Plant molecular diversity and application to genomics. Current Opinion in Plant Biology 5: 107-111. - Collard, B.C.Y. and Mackill, D.J., 2009. Start Codon Targeted (SCoT) polymorphism: a simple novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27: 86–93. - Davis, T.M., Haigis, Y.U.H. and McGowan, P.J., 1995. Template mixing: a method of enhancing detection and interpretation of codominant RAPD markers. Theoretical and Applied Genetics 91: 582-8 - Doyle, J.J. and Doyle J.L., 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull, 19:11–15. - Elazreg, H., Chtourou-Ghorbel, N., Ghariani, S., Chakroun, M. and Trifi-Farah, N., 2011. Studying genetic diversity of the Tunisian Lolium perenne and Festuca arundinacea with AFLP markers.Journal of Food, Agriculture & Environment, 9 (1): 409-415. - Farshadfar, M., Moradzade, N., Farshadfar, E. and Shirvani, H., 2017. Genetic diversity among fennel (Fueniculum vulgare Mill.) accessions using morphological and SCoT markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Researc. 25(2): 212-231. (In Farsi with English abstract). - Ghariani, S., Trifi-Farah, N., Chakroun, M., Marghali, S. and Marrakchi, M., 2003. Genetic diversity in Tunisian Perennial ryegrass revealed by ISSR markers. Genetic Resources and Crop Evolution, 50(8): 809-815. - Heydari Sharif Abad, H. and Dari, M.A., 2003. Fodder plants (grass) Volume II. Forestry and Rangeland Research Institute. Tehran. - Hou, Y., Yan, Z. and Wei, Y., 2005. Genetic diversity in barely from west China based on RAPD and ISSR analysis Barely. Genetics Newsletter, 35:9-22. - Humphreys, M.O., 1991. A genetic approach to the multivariate differentiation of perennial ryegrass (Lolium perenne L.) populations. Heredity, 66: 437-443. - Kalendar, R., 2007. FastPCR: a PCR primer design and repeat sequence searching software with additional tools for the manipulation and analysis of DNA and protein. Available at www.biocenter.helsinki.fi/programs/fastpcr.htm; 2007. - Kumar, M., Mishra, G.P., Singh, R., Kumar, J., Naik, P.K. and Singh S.B., 2009. Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of Trans-Himalayas. Physiology and Molecular Biology of Plants, 15(3): 225-236. - Majidi, M.M. and Mirlohi, A., 2010. Genetic similarities among Iranian populations of Festuca, Lolium, Bromus and Agropyron using amplified fragments length polymorphism (AFLP) markers. Iranian Journal of Biotechnology, 8(1): 16-23. - Moradi, P. and Jafari, A.A., 2006. Comparative study of forage quality in 26 Dactylis glomerata genotypes in order to produce synthetic varieties in Zanjan provienc. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 14: 175-180 (In Farsi with English abstract). - Peakall, R. and Smouse, P.E., 2006. GENALEX 6: Genetic Analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6:288-295. - Perrier, X. and Jacquemoud-Collet, J.P., (2006). DARwin soft ware, http://darwin.cirad.fr/darwin - Peters, J.P. and Martinelli, J.A., 1989. Hierarchical cluster analysis as a tool manages variation in germplasm collections. Theoretical and Applied Genetics, 78: 42-48. - Pivoriene, O. and Pasakinskiene, I., 2007. Genetic differences between annual and perennialryegrass revealed by ISSR markers and their Sequence characteristics. Zemdirbyste / Agriculture, 94: 197–204 - Pivoriene, O., Pašakinskiene, I., Brazauskas, G., Lideikyte. L., Jensen, L.B., and Lübberstedt, T., 2008. Inter-simple sequence repeats (ISSR) loci mapping in the genome of perennial ryegrass. Biologija. vol. 54. Nr. 1.P. 17–21 - Polok, K., Ciaglo, S. and Carbrita, L., 2006. AFLP analysis of genetic similarity between the species of the genus Lolium. Biodiv. Res. Conserv. 3-4: 232-234 - Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A., 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238. - Rohlf, F. and Taxonomy, N.P.N., 1998. Multivariate Analysis System, Version 2.02. New York: Exeter Software, Applied Biostatistics Inc. - Sicard, D., Nanni, L., Porfiri, O., Bulfon, D. and Papa, R., 2005. Genetic diversity of Phaseolus vulgaris L. and P. coccineus L. landraces in central Italy. Plant Breeding, 124 (5): 464–472. - Singh, S.K., 2003. Cluster analysis for heterosis in wheat (Triticum aestivum L.). Indian Journal of Genetics. 63(3):249-250. - Tsvelev, N.N., 1989. The system of grasses and their evolution. Botanical Review ,55: 141-204. - Vieira, E.A., Castro, C.M., Oliveira, A.Cd., Irajá, F., Carvalho, Fd., Zimmer, P.D. and Martins, L.F., 2004. Genetic structure of annual ryegrass (Lolium multiflorum) populations estimated by RAPD. Sci. Agric., 61(4): 407-413. - Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalskiand, J.A. and Tingey, S.V., 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, 18: 6531-6535. - Wright, S., 1949. The genetical structure of populations. Annals of Eugenics, 15: 323-354. | ||
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