Genetic diversity of several Iranian sumac (Rhus coriaria) populations using genomic inter-microsatellites markers | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 5, Volume 26, Issue 2 - Serial Number 52, December 2018, Pages 196-206 PDF (313.31 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijrfpbgr.2018.117959 | ||
| Authors | ||
| R. Mohammadi Alaghoz1; R. Darvish zadeh* 2; A. Alijanpour3; H. Hatami Maleki4; R. Heidari5 | ||
| 1M.Sc., Plant Breeding, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I. R. Iran. | ||
| 2Corresponding author, Prof., Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I.R. Iran. Email: r.darvishzadeh@urmia.ac.ir | ||
| 3Assoc. Prof., Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I. R. Iran. | ||
| 4Assist. Prof., Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, I. R. Iran. | ||
| 5M.Sc., Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, I. R. Iran. | ||
| Abstract | ||
| DOR: 98.1000/1735-0891.1397.2.196.52.26.1576.82 Sumac (Rhus coriaria L.) as a forest shrub has been dispersed in Mediterranean and Eastern Asia specially Iran and has several medicinal and industrial applications. In this research, intermicrosatellites markers were used to study genetic diversity of sumac genotypes collected from five natural habitats located in North West of Iran (West and East Azerbaijan provinces). Fifteen samples were select randomly from each population and genomic DNA was fingerprinted by 18 ISSR markers. Results revealed existence of suitable genetic variation among the studied sumac genotypes and portion of inter population (79.8%) variation were higher than intra population (20.2%) variation. Minimum value of genetic distance (14.18) was observed between Aghberaz-Horand (East Azerbaijan province) and Nir-Arasbaran (East Azerbaijan province) populations and the maximum one (31.08) was observed between Kachelleh-Urmia (West Azerbaijan) and Nir-Arasbaran populations. According to classification of genotypes based on molecular data, Aghberaz-Horand and Nir-Arasbaran populations located in the same group and populations from other habits located in distinguished groups. Classification of genotypes via ISSR markers was in agreement with their geographical distributions. ISSR markers could be effectively applicable in genetic evaluation of sumac germplasm and also in identification of genetic relatedness among known and unknown sumac samples. | ||
| Keywords | ||
| Genetic diversity; ISSR molecular markers; molecular analysis of variance; Sumac | ||
| References | ||
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− Abdi, N. and Maddah-Arefi, H., 2002. Study of variation and seed deterioration of Bromus tomentollus germplasm, in natural resources gene bank. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 7: 1-25. − Ahmadian Atari, M.M., Amin, G.R., Fazeli, M.R. and Jamalifar, H., 2008. A review on antimicrobial activities of sumac fruit (Rhus coriaria L.). Journal of Medicinal Plants, 25: 1-9. − Avise, J.C., 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall. New York. 528p. − Belletti, P., Monteleone, I. and Ferrazzini, D., 2008. A population genetic study in a scattered forest species, Wild service tree [Sorbus torminalis (L.) crantz], using RAPD markers. European Journal of Forest Research, 127: 103-114. − Bernath, J., 2002. Strategies and recent achievements in selection of medicinal and aromatic plants. Proc. Int. Conf. MAP. Acta Horticulturae, 576, 65– 68. − Ellstrand, N.C. and Elam, D.R., 1993. Population genetic consequences of small population size: implications for plant conservation. Annual Reviews of Ecological Systems, 24: 217–242. − Fabriki Ourang, S., Shamsbakhsh, M., Jalali Javaran, M. and Ahmadi, J., 2009. Assessment of genetic diversity of native populations of Iranian melon (Cucumis melo L.) using inter simple sequence repeats (ISSR) markers. Journal of Iranian Biology, 22: 271-278. − Fritsch, P. and Rieseberg, L.H., 1996. The use of random amplified polymorphic DNA (RAPD) in conservation genetics. In: T.B. Smith and R.K. Wayne, Editors, Molecular Genetic Approaches in Conservation, Oxford University Press. New York. USA. pp. 54-73. − Ghouth, K., Malekzadeh Shafaroodi, S., Rashed Mohassel, M.H., Akbari, M.R. and Razavi, S.H., 2014. Grouping jujubes of Iran based on quantitative characteristics and ISSR and RAPD Markers. Seed and Plant Improvement Journal, 30: 173-190. − Gonzalez-Astorga, J. and Castillo-Campos, G., 2004. Genetic variability of the narrow endemic tree Antirhea aromatica Castilo-Campos and Lorence, (Rubiaceae, Guettardeae) in a tropical forest of Mexico. Annals of Botany, 93: 521–528. − Hamrick, J.L. and Godt, M.J.W., 1996. Conservation genetics of endemic plant species. In: Avise, J.C., Hamrick, J. L. (Eds.), Conservation Genetics: Case Histories from Nature. Chapman and Hall, New York. − Hamrick, J.L., Linhart, Y.B. and Mitton, J.B., 1979. Relationships between life history characteristics and electrophoretically detectable genetic variance in plants. Annual Reviews of Ecological Systems, 10:173–200. − Hao, B., Li, W., Linchun, M., Li, Y., Rui, Z., Mingxia, T. and Weikai, B., 2006. A study of conservation genetics in Cupressus chengiana an endangered endemic of China, using ISSR markers. Biochemical Genetics, 44: 29-43. − Hartl, D.L. and Clark, G.C., 1994. Principles of Population Genetics. Sinauer, Sunderland. − Huang, Y., Ji, K., Jiang, Z. and Tang, G., 2008. Genetic structure of Buxus sinica var. parvifolia, a rare and endangered plant. Scientia Horticulturae, 116: 324-329. − Koohi Dehkordi, M.A., Rahim Malek, M., Seyyed Tabtabei, B.A., Bani Nasab, B. and Mobli, M., 2006. Assessment of genetic relationship of some Iranian and exotic olive cultivars using molecular markers. Journal of Horticultural Science and Technology, 7: 93-102. − Lu, Z., Wang, Y., Peng, Y., Korpelainen, H. and Li, C., 2006. Genetic diversity of Populus cathayana Rehd populations in southwestern China revealed by ISSR markers. Plant Science, 170: 407-412. − Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590. − Rawashdeh I.M., 2011. Genetic variability in a medicinal plant Artemisia judaica using random amplified polymorphic DNA (RAPD) markers. International Journal of Agriculture and Biology, 13: 279-282. − Rawashdeh I.M., Ghzawi A.L., Rawasgdeh, N.Q., Kheirallh, K., Al-Tawaha, A.R. and Salama, B., 2009. Genetic variation among sumac (Rhus Coriaria L.) samples collected from three locations in Jordan as revealed by AFLP markers. Advances in Environmental Biology, 3: 107-112. − Reddy, P.M., Sarla, N. and Siddiq, E.A., 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9–17. − Reed, D.H. and Frankham, R., 2003. Correlation between fitness and genetic diversity. Conservational Biology, 17: 230-237. − Rout, A. and Chrungoo, N.K., 2007. Genetic variation and species relationships in Himalayan buck wheats as revealed by SDS PAGE of endosperm proteins extracted from single seeds and RAPD based DNA fingerprints. Genetic Resources and Crop Evolution, 54: 767–777. − Shabanian, N., Havasi, A. and Mehrabi, A.A., 2016. Genetic differentiation in Persian oak (Quercus brantii) populations using genomic inter-microsatellite markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24: 66-78. − Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, M., 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739. − Yeh, F.C., Yang, R. and Boyle, T., 1999. POPGENE Version 1.31. Microsoft windows based freeware for population genetic analysis. University of Alberta. Edmonton. AB. Canada. pp. 26. − Zietkiewicz, E., Rafalski, A. and Labuda, D., 1994. Genome fingerprinting by simple sequence repeat (SSR) - anchored polymerase chain reaction amplification. Genomics, 20: 176-183. | ||
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