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استخراج تریترپنوئیدهای ضدسرطانی (اسید بتولینیک و بتولین) از گلسنگ (Ramalina sinensis) پوستزی درخت توس (.Betula pendula Roth) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 34، شماره 4 - شماره پیاپی 90، مهر و آبان 1397، صفحه 604-616 اصل مقاله (364.06 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.120476.2245 | ||
| نویسندگان | ||
| جمیله نظری1؛ وحیده پیام نور* 2؛ محمدرضا کاوسی2؛ جهانبخش اسدی3 | ||
| 1دانشجوی دکترای جنگلشناسی و اکولوژی جنگل، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 2دانشیار، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 3دانشیار، گروه بیوشیمی، دانشکده پزشکی، دانشگاه علوم پزشکی گلستان، گرگان، ایران | ||
| چکیده | ||
| گلسنگها زیستبومهای کوچکی هستند که شامل دو موجود همزیست قارچی و جلبکی میباشند. آنها بهعنوان یکی از منابع سرشار از ترکیبهای طبیعی با خاصیت آنتیتوموری، آنتیبیوتیکی و همچنین آنتیاکسیدانی شناخته شدهاند که از برخی از آنها بهعنوان دارو و برای درمان بیماریهای خاص استفاده میشود. این پژوهش با هدف شناسایی و تعیین میزان بتولین، بتولینیک اسید و ارزیابی فعالیت آنتیاکسیدانی گلسنگ پوستزی درخت توس (.Betula pendula Roth) و همچنین قارچ همراه آن انجام شد. مشخص گردید که گلسنگ جدا شده از پوست این درخت، گلسنگهای برگی دارای ترکیبهای بیواکتیو، تحت عنوان Ramalina sinensis میباشد. قارچ همراه با گلسنگ حاوی ماده مؤثره با روش مولکولی Arthrinium arundinis شناسایی شد. این قارچ جزء قارچهای آسکومیست بوده و برای اولین بار با کد MG198621 در سایت NCBI ثبت گردید. از گلسنگ و قارچ همراه آن عصارهگیری شد و با استفاده از دستگاه HPLC میزان اسید بتولینیک و بتولین آن تعیین شد. اسید بتولینیک و بتولین جزء ترپنها بوده و بهعنوان عامل ضد سرطانی قوی شناخته شده است. در بافت گلسنگ R. sinensis (بهترتیب 2.17% و 0.075%) و در قارچ همراه آن A. arundinis (بهترتیب 1.6% و 0.025%) به میزان قابل توجهی اندازهگیری شد. به این ترتیب گلسنگ و قارچ همراه آن برای نخستین بار بهعنوان منابع جدید حاوی این دو ماده مؤثره معرفی میشوند. اندازهگیری خاصیت آنتیاکسیدانی عصاره گلسنک و قارچ همراه آن با استفاده از دو حلال متانول و اتانول آزمون شد. مشخص گردید که عصاره گلسنگ و قارچ همراه آن، علاوهبر داشتن متابولیتهای ثانویه با ارزش دارای خاصیت آنتیاکسیدان بوده که توانایی حذف رادیکالهای آزاد سمّی را دارند. این نتایج میتواند در عرصه پزشکی و صنعت اثرهای ارزندهای داشته باشد. | ||
| کلیدواژهها | ||
| اسید بتولینیک؛ بتولین؛ آنتیاکسیدان؛ توس (.Betula pendula Roth)؛ گلسنگ | ||
| عنوان مقاله [English] | ||
| Extraction of anti-cancer triterpenoids (betulinic acid and betulin) from the birch bark-inhabiting lichen (Ramalina sinensis) | ||
| نویسندگان [English] | ||
| J. Nazari1؛ V. Payamnoor2؛ M.R. Kavosi2؛ J. Asadi3 | ||
| 1Ph.D. student of Sylviculture and Forest Ecology, Faculty of Forestry, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran | ||
| 2Faculty of Forestry, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran | ||
| 3Department of Clinical Biochemistry, Golestan University of Medical Sciences, Gorgan, Iran | ||
| چکیده [English] | ||
| Lichens are small environments containing two fungus and algae symbiosis. They are one of the richest sources of natural ingredients with anti-tumor, antibiotic, and antioxidant properties, some of which are used as drugs and for the treatment of certain diseases. This research was aimed to identify and determine the amount of betolin and betulinic acid as well as evaluation of the antioxidant activity of lichen Betula pendula Roth. Roth.and its symbiotic fungus. It was found that the lichen isolated from the bark of birch contained bioactive compounds entitled Raminalina sinensis. The symbiotic fungus (Arthrinium arundinis) was detected by the molecular method. This fungus belongs to the ascomycetes, registered for the first time in the NCBI website with MG198621 code. In the extract of lichen and its symbiotic fungus, the amount of betulinic acid and betulinic acid was determined using HPLC. Betulinic acid and betulin, belonging to the terpenes, are known as a strong anticancer agent. The mentioned ingredientswere significantly found in the lichen tissue(2.17 and 0.075 percent, respectively) and in its symbiotic fungus A. arundinis (1.6 and 0.025 percent, respectively). In this way, the lichen and its symbiotic fungus are introduced for the first time as new sources containing these two active ingredients. Measurement of antioxidant properties of lichen extract and its symbiotic fungus was examined by methanol and ethanol solvents. It was found that the lichen extract and the symbiotic fungus, besides having alternative metabolites, have antioxidant properties, which are able to remove toxic free radicals. These results could have valuable effects in medicine and industry. | ||
| کلیدواژهها [English] | ||
| Betulinic acid, betulin, Antioxidant, birch (Betula pendula Roth.), lichen | ||
| مراجع | ||
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