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بهینه سازی روشهای افزایش کارایی جوانهزنی و سبز شدن گیاه دارویی گل راعی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 8، دوره 7، شماره 1 - شماره پیاپی 13، مرداد 1397، صفحه 95-108 اصل مقاله (969.7 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.117055 | ||
| نویسندگان | ||
| محمدرضا نعمتی خویی1؛ علی عباسی سورکی* 2؛ سینا فلاح3 | ||
| 1دانش آموخته کارشناسی ارشد علوم و تکنولوژی بذر دانشگاه شهرکرد | ||
| 2عضو هیئت علمی دانشگاه شهر کرد، ایران. | ||
| 3عضو هیئت علمی دانشگاه شهرکرد، ایران. | ||
| چکیده | ||
| به منظور تعیین بهترین روش افزایش کارایی جوانهزنی و رشد اولیه گیاهچه گیاه دارویی گل راعی (Hypericum perforatum L.) آزمایشی در دو مرحله در دانشگاه شهرکرد انجام شد. ابتدا آزمایش به صورت فاکتوریل در قالب طرح بلوکهای کامل تصادفی با چهار تکرار درآزمایشگاه علوم و تکنولوژی بذر دانشکده کشاورزی انجام گرفت. تیمارهای آزمایشی شامل پیش تیمار بذر با پلی اتیلن گلایکول 6000 در پنج سطح (صفر، 3-، 6-، 9- و 12- بار)به عنوان فاکتور اول و کاربرد هورمون جیبرلین در چهار سطح (صفر، 500، 1000 و 1500 پیپیام) به عنوان فاکتور دوم بودند. نتایج نشان داد تیمار PEG -12 bar + GA 1000 ppm با میانگین 91.5 درصد بالاترین درصد جوانهزنی را به خود اختصاص داد که با تیمارهای PEG -9 bar + GA 1500 ppm و PEG -9 bar + GA 1000 ppm به ترتیب با میانگین 88.5 و 88 درصد جوانهزنی تفاوت معنی داری نداشت. درحالیکه بذرهای شاهد با میانگین 5/53 پایینترین درصد جوانهزنی را داشتند. تیمار PEG -9 bar + GA 1000 ppm دارای بیشترین طول گیاهچه، وزن خشک گیاهچه، بنیه بذر یک و دو بود و میزان این پارامترها را به ترتیب به اندازه 1.19، 1.3، 1.96 و 2.14 برابر نسبت به شاهد افزایش داد . آزمایش دوم در قالب طرح بلوکهای کامل تصادفی با سه تکرار در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه شهرکرد انجام شد. تیمارهای استفاده شده در این آزمایش شامل بهترین تیمارهای آزمایش اول به همراه شاهد بودند. بررسی نتایج گلخانهای نشان داد تیمار PEG -12 bar + GA 1000 ppm با میانگین 82 درصد بالاترین درصد سبز شدن را به خود اختصاص داد که نسبت به شاهد با میانگین 41.33 درصد عملکرد بهتری داشت. لذا پیشنهاد میشود به منظور بهینه سازی و افزایش کارایی جوانهزنی بذر گیاه دارویی گل راعی از پیشتیمار PEG -12 bar + GA 1000 ppm و PEG -9 bar + GA 1000 ppm استفاده گردد. | ||
| کلیدواژهها | ||
| گل راعی؛ جیبرلین؛ پلی اتیلن گلایکول؛ پرایمینگ؛ بنیه بذر | ||
| عنوان مقاله [English] | ||
| Optimization of seed enhancement methods on seed germination and emergence of St. John’s wort | ||
| نویسندگان [English] | ||
| M. Nemati Khoei1؛ A. Abbasi Surki2؛ S. Fallah3 | ||
| 1M.Sc. Graduated of seed Science and Technology of Shahrekord University | ||
| 2Faculty member of Shahrekord University, Shahrekord, Iran. | ||
| 3Faculty members of Shahrekord University Shahrekord, Iran. | ||
| چکیده [English] | ||
| Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed science and Technology lab, Faculty of Agriculture. Treatments consisted osmopriming with PEG in five levels (0, -3, -6, -9 and -12 bar) as first factor and application of GA in four levels (0, 500, 1000 and 1500 ppm) as second factor. results showed that PEG -12 bar + GA 1000 ppm with an average of 91.5% ranked the highest percentage of germination which had no significant difference with PEG -9 bar + GA 1500 ppm and PEG -9 bar + GA 1000 ppm respectively with the germination percentage average 88 and 88.5%, while the control seed with an average (53.5%) had the lowest percentage of germination. As well as combination treatments PEG -9 bar + GA 1000 ppm increased seedling length, seedling dry weight and seed vigor and increased them respectively as by as 1.19, 1.3, 1.96 and 2.14 times than control. The second experiment was conducted in a randomized complete block design with three replications in the research greenhouses conditions, and treatments consisted of the best treatments of first experiment and control. In greenhouse conditions osmopriming PEG -12 bar and application of GA 1000 ppm with an average of 82% ranked the highest percentage of emergence which had better performance than the control with an average of 41.33 %. Finally to enhance seed performance of medicinal plant Hypericum perforatum L., it could be recommended osmopriming with PEG -12 bar and application of GA 1000 ppm or PEG -9 bar + GA 1000 ppm. | ||
| کلیدواژهها [English] | ||
| Hypericum perforatum L, Gibberellin, Polyethylene Glycol, priming, Seed vigor | ||
| مراجع | ||
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